TBLASTN 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= SPP00000074_2.0 # Protein # Selenoprotein I (SelI) # Human
(Homo sapiens)
         (397 letters)

Database: genome.fa 
           133,062 sequences; 684,497,465 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BADN01047755.1 Thunnus orientalis DNA, contig: superscaffoldBa00...   141   1e-71
BADN01095793.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    69   4e-11

>BADN01047755.1 Thunnus orientalis DNA, contig: superscaffoldBa00001382_0012, whole
            genome shotgun sequence
          Length = 9087

 Score =  141 bits (355), Expect(4) = 1e-71,   Method: Compositional matrix adjust.
 Identities = 66/94 (70%), Positives = 73/94 (77%)
 Frame = -3

Query: 98   FVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIFGRGSTGXXXXXXX 157
            F+ + +DGVDGKQARRTNSSTPLGELFDHGLDSW+C++FV TVYSIFGRG +G       
Sbjct: 1192 FLCFCVDGVDGKQARRTNSSTPLGELFDHGLDSWACIFFVATVYSIFGRGESGVGVATLY 1013

Query: 158  XXXXXXXXXXILSHWEKYNTGILFLPWGYDISQV 191
                      ILSHWEKYNTGILFLPWGYDISQV
Sbjct: 1012 YILWVVLFSFILSHWEKYNTGILFLPWGYDISQV 911



 Score =  109 bits (273), Expect(4) = 1e-71,   Method: Compositional matrix adjust.
 Identities = 64/168 (38%), Positives = 84/168 (50%), Gaps = 39/168 (23%)
 Frame = -2

Query: 239 LLNFFRSYKNNTLKLNSVYEAMVPLFSPCLLFILSTAWILWSPSDILELHPRVFYFMVGT 298
           LL F R++++NTLK +S+YEA +P  SP LLF+LST W+++SPS+ILEL PR+FY MVGT
Sbjct: 503 LLYFRRAHRSNTLKHSSLYEAFLPFLSPVLLFVLSTIWVVYSPSNILELQPRIFYLMVGT 324

Query: 299 AFANST---------------------------------------CQLIVCQMSSTRCPT 319
           AFAN T                                       C+LIVCQMS+TRC  
Sbjct: 323 AFANVTVSLLMSHSHKHTVSHKAYISVFRC*CIFIHMCVCVFILQCKLIVCQMSNTRCQP 144

Query: 320 LNWXXXXXXXXXXXXXXXXASYVESIXXXXXXXXXXXXHIHYGVRVVK 367
           L+W                 +  E++            HIHYGV VV+
Sbjct: 143 LSWLLLPMTPVVLLAVTGVVAN-ETLLLYLWTAAVILAHIHYGVSVVR 3



 Score = 62.0 bits (149), Expect(4) = 1e-71,   Method: Compositional matrix adjust.
 Identities = 31/41 (75%), Positives = 34/41 (82%)
 Frame = -2

Query: 188 ISQVTISFVYIVTAVVGVEAWYEPFLFNFLYRDLFTAMIIG 228
           IS  TIS VY+VTAVVGVE WY+P L +FLYRDLFT MIIG
Sbjct: 827 ISFQTISLVYLVTAVVGVETWYQPVLLHFLYRDLFTFMIIG 705



 Score = 21.9 bits (45), Expect(4) = 1e-71,   Method: Compositional matrix adjust.
 Identities = 9/17 (52%), Positives = 11/17 (64%)
 Frame = -1

Query: 228 GCALCVTLPMSLLNFFR 244
            C+  VTLPMSL N  +
Sbjct: 627 ACSFTVTLPMSLYNVLK 577


>BADN01095793.1 Thunnus orientalis DNA, contig: superscaffoldBa00005213_0010, whole
            genome shotgun sequence
          Length = 2004

 Score = 68.6 bits (166), Expect = 4e-11,   Method: Compositional matrix adjust.
 Identities = 33/60 (55%), Positives = 40/60 (66%)
 Frame = -3

Query: 84   HVPDWVWIVVGILNFVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSI 143
              P W +++  +  FV  +LD +DGKQARRTNSS+PLGELFDHG DS S   FV    SI
Sbjct: 1615 QAPLWAYLLCAVGLFVYQSLDAIDGKQARRTNSSSPLGELFDHGCDSLSTG*FVFLFASI 1436


  Database: genome.fa
    Posted date:  Oct 9, 2014  7:00 PM
  Number of letters in database: 684,497,465
  Number of sequences in database:  133,062
  
Lambda     K      H
   0.327    0.141    0.463 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 133062
Number of Hits to DB: 345,756,945
Number of extensions: 5527612
Number of successful extensions: 38671
Number of sequences better than 1.0e-09: 2
Number of HSP's gapped: 38661
Number of HSP's successfully gapped: 6
Length of query: 397
Length of database: 228,165,821
Length adjustment: 123
Effective length of query: 274
Effective length of database: 211,799,195
Effective search space: 58032979430
Effective search space used: 58032979430
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 43 (21.2 bits)