TBLASTN 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= SPP00000054_2.0 # Protein # SECIS binding protein 2 (SBP2) #
Human (Homo sapiens)
         (854 letters)

Database: genome.fa 
           133,062 sequences; 684,497,465 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BADN01086679.1 Thunnus orientalis DNA, contig: superscaffoldBa00...   119   5e-26
BADN01061254.1 Thunnus orientalis DNA, contig: superscaffoldBa00...   109   7e-23

>BADN01086679.1 Thunnus orientalis DNA, contig: superscaffoldBa00004124_0004, whole
            genome shotgun sequence
          Length = 5612

 Score =  119 bits (299), Expect = 5e-26,   Method: Compositional matrix adjust.
 Identities = 55/74 (74%), Positives = 61/74 (82%)
 Frame = +2

Query: 632  YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVXXXXXXXXXXC 691
            YCSQMLSK+VD CVT LLKELVRFQDR+YQKDP+KA+ KRRLV+GLREV          C
Sbjct: 1979 YCSQMLSKDVDECVTSLLKELVRFQDRLYQKDPMKARMKRRLVMGLREVLKHLKLRKVKC 2158

Query: 692  VIISPNCEKIQSKG 705
            VIISPNCE+IQSKG
Sbjct: 2159 VIISPNCERIQSKG 2200



 Score =  103 bits (256), Expect = 6e-21,   Method: Compositional matrix adjust.
 Identities = 59/159 (37%), Positives = 82/159 (51%), Gaps = 43/159 (27%)
 Frame = +2

Query: 705  GGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ-------- 756
            GGLD+ LHTIID   EQ +PFVFAL+RKALGR +NKAVPVS+VGIF+YDGAQ        
Sbjct: 2426 GGLDEALHTIIDTCREQGVPFVFALSRKALGRCVNKAVPVSLVGIFNYDGAQVSFMLNSS 2605

Query: 757  -----------------------------------DQFHKMVELTVAARQAYKTMLENVQ 781
                                               D +HKM+EL+  AR+AY+ M+ +++
Sbjct: 2606 TH*QRVRQFL**CF*Q*KIISVFQTG*KLYPSSLQDYYHKMIELSSEARKAYEVMVSSLE 2785

Query: 782  QELVGEPRPQAPPSLPTQGPSCPAEDGPPALKEKEEPHY 820
            Q    +   +   ++     S  +E+  P   ++EEP Y
Sbjct: 2786 Q--TNQSDAEQAANIEINELSL-SEEPDPNTTQQEEPEY 2893



 Score = 57.0 bits (136), Expect(2) = 1e-08,   Method: Compositional matrix adjust.
 Identities = 27/36 (75%), Positives = 32/36 (88%)
 Frame = +1

Query: 500  QMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKI 535
            Q K  HNPLDS++PL+KKGKQRE+PKAKKPT LKK+
Sbjct: 1120 QDKIAHNPLDSTSPLVKKGKQREVPKAKKPTPLKKV 1227



 Score = 26.6 bits (57), Expect(2) = 1e-08,   Method: Compositional matrix adjust.
 Identities = 13/23 (56%), Positives = 18/23 (78%), Gaps = 2/23 (8%)
 Frame = +3

Query: 534  KIILKERQERKQR--LQENAVSP 554
            ++ILKER+ERKQR  L+E  + P
Sbjct: 1332 QVILKEREERKQRRLLEERGLLP 1400


>BADN01061254.1 Thunnus orientalis DNA, contig: superscaffoldBa00002090_0009, whole
            genome shotgun sequence
          Length = 9909

 Score =  109 bits (273), Expect = 7e-23,   Method: Compositional matrix adjust.
 Identities = 51/74 (68%), Positives = 60/74 (81%)
 Frame = +3

Query: 632  YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVXXXXXXXXXXC 691
            YC+Q+LSKE+D  VT LL+ELVRFQ+R+YQKDP KAK+KRRLV+GLREV          C
Sbjct: 1686 YCNQVLSKEIDESVTLLLQELVRFQERVYQKDPTKAKSKRRLVMGLREVTKHMKLHKIKC 1865

Query: 692  VIISPNCEKIQSKG 705
            VIISPNCEKIQ+KG
Sbjct: 1866 VIISPNCEKIQAKG 1907



 Score = 77.0 bits (188), Expect = 9e-13,   Method: Compositional matrix adjust.
 Identities = 35/52 (67%), Positives = 42/52 (80%)
 Frame = +1

Query: 705  GGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ 756
            GGLD+ L+ +I  A EQ IPFVFAL RKALGR +NK VPVSVVGIF++ GA+
Sbjct: 2086 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNFSGAE 2241



 Score = 52.4 bits (124), Expect = 3e-05,   Method: Compositional matrix adjust.
 Identities = 22/32 (68%), Positives = 29/32 (90%)
 Frame = +1

Query: 504 PHNPLDSSAPLMKKGKQREIPKAKKPTSLKKI 535
           PHN LDS+AP +K+GK+REIPK K+PT+LKK+
Sbjct: 136 PHNALDSTAPRVKRGKEREIPKVKRPTALKKV 231


  Database: genome.fa
    Posted date:  Oct 9, 2014  7:00 PM
  Number of letters in database: 684,497,465
  Number of sequences in database:  133,062
  
Lambda     K      H
   0.311    0.128    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 133062
Number of Hits to DB: 545,359,829
Number of extensions: 7105178
Number of successful extensions: 26995
Number of sequences better than 1.0e-02: 3
Number of HSP's gapped: 26957
Number of HSP's successfully gapped: 9
Length of query: 854
Length of database: 228,165,821
Length adjustment: 130
Effective length of query: 724
Effective length of database: 210,867,761
Effective search space: 152668258964
Effective search space used: 152668258964
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 46 (22.3 bits)