TBLASTN 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= sp|Q503H1|TSAP1_DANRE tRNA selenocysteine 1-associated protein
1 OS=Danio rerio GN=trnau1ap PE=2 SV=1
         (314 letters)

Database: genome.fa 
           133,062 sequences; 684,497,465 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BADN01022579.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    84   1e-16
BADN01039777.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    52   8e-06
BADN01065248.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    45   7e-04

>BADN01022579.1 Thunnus orientalis DNA, contig: superscaffoldBa00000445_0022, whole
             genome shotgun sequence
          Length = 19638

 Score = 84.3 bits (207), Expect = 1e-16,   Method: Compositional matrix adjust.
 Identities = 53/130 (40%), Positives = 64/130 (49%), Gaps = 46/130 (35%)
 Frame = +2

Query: 91    SSTFSLFVSDLTPDVDDGMLYEFFHYHFSSCCSGKIVLDANGHS-------------KCC 137
             S  +SLFV DLTP+VDDGMLYEFF+  + SC  GK+VLD+ G+S             K C
Sbjct: 12005 SQMYSLFVGDLTPEVDDGMLYEFFYNRYPSCRGGKVVLDSMGNSK*VCFNMSPHNMEKIC 12184

Query: 138   G---------------------------------FVSFESEREQKRALVDLQGATGLGKK 164
                                               FV F  ER QKRAL + QGA GLG K
Sbjct: 12185 N*YRLLSQNLSQ*SVFI*NSHTSSVCVCCCRGCGFVQFPDERLQKRALEECQGAVGLGAK 12364

Query: 165   ALRLSLASSR 174
              LRLSLA+++
Sbjct: 12365 PLRLSLAANK 12394



 Score = 52.4 bits (124), Expect(2) = 5e-15,   Method: Compositional matrix adjust.
 Identities = 22/33 (66%), Positives = 27/33 (81%)
 Frame = +3

Query: 9     LEPYMDEDFICRAFAQMGETVVKIRLIRDKITG 41
             LE YMDE FI RAF+ MGE VV +R+IR+K+TG
Sbjct: 11463 LEAYMDEKFITRAFSTMGEQVVNVRIIRNKMTG 11561



 Score = 50.8 bits (120), Expect(2) = 5e-15,   Method: Compositional matrix adjust.
 Identities = 20/30 (66%), Positives = 23/30 (76%)
 Frame = +2

Query: 45    GYGFVELADDTAVERCLRKVNGKPLPGATP 74
             GY FVE+ D+   ERCLRK+NGK LPGA P
Sbjct: 11648 GYCFVEMTDEATAERCLRKINGKALPGANP 11737



 Score = 47.8 bits (112), Expect = 1e-04,   Method: Compositional matrix adjust.
 Identities = 27/51 (52%), Positives = 31/51 (60%)
 Frame = +2

Query: 248   PDSEINVTEANETFMAQSEELYSALIGCFFQPPESWDGVTCSASSYLPEPI 298
             P  E++V EAN  FM  SEELY ALI C +QP E      C  S+ LPEPI
Sbjct: 14198 PSPELDVVEANRKFMELSEELYDALIECHWQPAEFSTEQECMTSN-LPEPI 14347


>BADN01039777.1 Thunnus orientalis DNA, contig: superscaffoldBa00001026_0023, whole
             genome shotgun sequence
          Length = 20293

 Score = 51.6 bits (122), Expect = 8e-06,   Method: Compositional matrix adjust.
 Identities = 29/78 (37%), Positives = 45/78 (57%), Gaps = 5/78 (6%)
 Frame = +1

Query: 44    AGYGFVELADDTAVERCLRKVNGKPLPGATPPKRFKLSRSNYGKQGESSTFS----LFVS 99
             AGY FVELAD+ +V+RC++++NGK +PG+ P     L ++ Y      +TF     LF+ 
Sbjct: 15706 AGYCFVELADEASVDRCVQRLNGKLVPGSNPVSSMLLLQNTY*YVF*CTTFRNI*LLFLH 15885

Query: 100   DLTPDVDDGML-YEFFHY 116
                  +D+ +L   FF Y
Sbjct: 15886 KYINSIDNLILPSSFFQY 15939


>BADN01065248.1 Thunnus orientalis DNA, contig: superscaffoldBa00002340_0009, whole
            genome shotgun sequence
          Length = 3032

 Score = 45.4 bits (106), Expect = 7e-04,   Method: Compositional matrix adjust.
 Identities = 20/67 (29%), Positives = 38/67 (56%)
 Frame = +1

Query: 3    SLWMGNLEPYMDEDFICRAFAQMGETVVKIRLIRDKITGKNAGYGFVELADDTAVERCLR 62
            ++++GNL+P +DE  +   F+  G  +   +++RD  TG + GY F+  A   A +  + 
Sbjct: 1762 NIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIE 1941

Query: 63   KVNGKPL 69
             +NG+ L
Sbjct: 1942 AMNGQYL 1962


  Database: genome.fa
    Posted date:  Oct 9, 2014  7:00 PM
  Number of letters in database: 684,497,465
  Number of sequences in database:  133,062
  
Lambda     K      H
   0.314    0.131    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 133062
Number of Hits to DB: 276,592,995
Number of extensions: 4026116
Number of successful extensions: 15323
Number of sequences better than 1.0e-02: 3
Number of HSP's gapped: 15321
Number of HSP's successfully gapped: 7
Length of query: 314
Length of database: 228,165,821
Length adjustment: 121
Effective length of query: 193
Effective length of database: 212,065,319
Effective search space: 40928606567
Effective search space used: 40928606567
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 42 (20.8 bits)