TBLASTN 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= SPP00000662_2.0 # Protein # Eukaryotic elongation factor
(eEFsec) # Zebrafish (Danio rerio)
         (576 letters)

Database: genome.fa 
           133,062 sequences; 684,497,465 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BADN01010204.1 Thunnus orientalis DNA, contig: superscaffoldBa00...   307   1e-89
BADN01010202.1 Thunnus orientalis DNA, contig: superscaffoldBa00...   150   9e-37
BADN01010203.1 Thunnus orientalis DNA, contig: superscaffoldBa00...   123   6e-28

>BADN01010204.1 Thunnus orientalis DNA, contig: superscaffoldBa00000153_0033, whole
           genome shotgun sequence
          Length = 10452

 Score =  307 bits (786), Expect = 1e-89,   Method: Compositional matrix adjust.
 Identities = 151/224 (67%), Positives = 179/224 (79%), Gaps = 6/224 (2%)
 Frame = +2

Query: 254 KVTRKVKSVQMFRKPVASAMQGDRVGVCVTQFDPKLLERGVVCTPGSLQTLYAAIISVQK 313
           +VT+KVKSVQMFRKPV+ AMQGDRVGVCVTQFDPKLLERGVVCTPGSL TLYAA++SV+K
Sbjct: 404 QVTKKVKSVQMFRKPVSGAMQGDRVGVCVTQFDPKLLERGVVCTPGSLHTLYAAVVSVRK 583

Query: 314 IEYYRGALNSRAKFHITVGHETVMARISFFNRVLP----ANENGDSKPTSQEQSANS-FS 368
           I Y++G+L +RAKFHITVGHETVMAR++FF   LP    +    D+KP  +  S  + F+
Sbjct: 584 IGYFKGSLATRAKFHITVGHETVMARVTFFG--LPPVGVSEPPSDTKPPPEPCSLETPFT 757

Query: 369 FDWEFHHLDEHLTSQAEGGKG-EPQQWALLEFERPVTCPPLCLVIGSRLDSDIHGNTCRL 427
           FD E+ + D +LT Q E   G +P+QWALLEFERPVTCP LCLVIGS+LD+DIH N CRL
Sbjct: 758 FDREYFYQDAYLTGQGEASSGPDPEQWALLEFERPVTCPSLCLVIGSKLDTDIHANACRL 937

Query: 428 AFHGKLLEGFEDKNYTETALPRLKISKDKQKEGAVERVTDDYTV 471
           AF G+LL+GFEDK Y ETALPRL+I K K KEG VERV+   TV
Sbjct: 938 AFQGRLLQGFEDKGYAETALPRLRIYKTKHKEGQVERVSGH*TV 1069



 Score = 97.8 bits (242), Expect = 1e-19,   Method: Compositional matrix adjust.
 Identities = 47/55 (85%), Positives = 50/55 (90%)
 Frame = +1

Query: 461  AVERVTDDYTVIGRNLFKKETNLQLFVGLKVTLSTGEAGSIEGGFGQSGKIKIRI 515
            +  +VTDDYTVIGRNLFKKETNLQLFVGLKVTLSTGE G IEGGFGQSGK KIR+
Sbjct: 3214 SCSQVTDDYTVIGRNLFKKETNLQLFVGLKVTLSTGETGVIEGGFGQSGKFKIRV 3378



 Score = 63.2 bits (152), Expect = 7e-09,   Method: Compositional matrix adjust.
 Identities = 32/60 (53%), Positives = 37/60 (61%)
 Frame = +3

Query: 517  EGLKEETKQLLXXXXXXXXXXXXXNESPKLEEAKPDAQPVTIHLNFKRYIYDPHKKMVQS 576
            EGL  ETKQLL                   EE K D+QPV+IHL+FKRY++DPHKKMVQS
Sbjct: 6003 EGLLPETKQLLSSTSKKKGKGGGKGGPANEEEPKADSQPVSIHLHFKRYVFDPHKKMVQS 6182


>BADN01010202.1 Thunnus orientalis DNA, contig: superscaffoldBa00000153_0031, whole
            genome shotgun sequence
          Length = 10557

 Score =  150 bits (380), Expect = 9e-37,   Method: Compositional matrix adjust.
 Identities = 70/93 (75%), Positives = 75/93 (80%)
 Frame = +2

Query: 6    KTLNFNVGVLGHVDSGKXXXXXXXXXXXXXXXFDKNPQSKERGITLDLGFSAFTVPLPEH 65
            KTLNFN+GVLGHVDSGK               FDKNPQS+ERGITLDLGFS+FTV LP H
Sbjct: 9890 KTLNFNIGVLGHVDSGKTSLARALSSTASTAAFDKNPQSRERGITLDLGFSSFTVDLPSH 10069

Query: 66   LRESCGEKQYDSLQFTLVDCPGHASLIRTIIGG 98
            LRES G++QYDSLQFTLVDCPGHASLIRTIIGG
Sbjct: 10070LRESLGQQQYDSLQFTLVDCPGHASLIRTIIGG 10168


>BADN01010203.1 Thunnus orientalis DNA, contig: superscaffoldBa00000153_0032, whole
            genome shotgun sequence
          Length = 3007

 Score =  123 bits (308), Expect = 6e-28,   Method: Compositional matrix adjust.
 Identities = 60/70 (85%), Positives = 66/70 (94%)
 Frame = +3

Query: 98   GAQIIDLMMLVVDVVKGMQTQTAECLLIGQLTCSRMVVILNKTDLLPSDKRQVAIDKMTK 157
            GAQIIDLMMLVVDVVKG+QTQTAECLLIG+LTC RMVVILNK DLLP++KRQ AI+KMTK
Sbjct: 1203 GAQIIDLMMLVVDVVKGVQTQTAECLLIGELTCPRMVVILNKIDLLPANKRQSAIEKMTK 1382

Query: 158  RMHKTLENTR 167
            R+HKTLE TR
Sbjct: 1383 RLHKTLEGTR 1412



 Score =  100 bits (249), Expect = 1e-20,   Method: Compositional matrix adjust.
 Identities = 48/62 (77%), Positives = 53/62 (85%)
 Frame = +3

Query: 197  LIDLLKAQSFLPHRDPSGSLLMAVDHCFSIRGQGTVITGTILQGSLSVNDNVEIPALKVT 256
            L  LLK Q++LP RDP G LLMAVDHCFSIRGQGTV+TGTILQGSL++ND VEIPALKV 
Sbjct: 2154 LSQLLKKQTYLPQRDPGGDLLMAVDHCFSIRGQGTVMTGTILQGSLAINDTVEIPALKVH 2333

Query: 257  RK 258
             K
Sbjct: 2334 FK 2339



 Score = 63.2 bits (152), Expect = 6e-09,   Method: Compositional matrix adjust.
 Identities = 28/42 (66%), Positives = 34/42 (80%)
 Frame = +3

Query: 167  RFKDCPIIAVAAKPGGPEAPDTDEPQGITELIDLLKAQSFLP 208
            RFK+CPIIAVAAKPGGPEA DT+EPQG+ ELI++    +  P
Sbjct: 1665 RFKECPIIAVAAKPGGPEAADTEEPQGVPELIEVKSGNTLEP 1790


  Database: genome.fa
    Posted date:  Oct 9, 2014  7:00 PM
  Number of letters in database: 684,497,465
  Number of sequences in database:  133,062
  
Lambda     K      H
   0.318    0.136    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 133062
Number of Hits to DB: 394,890,279
Number of extensions: 5397691
Number of successful extensions: 19289
Number of sequences better than 1.0e-02: 3
Number of HSP's gapped: 19280
Number of HSP's successfully gapped: 7
Length of query: 576
Length of database: 228,165,821
Length adjustment: 127
Effective length of query: 449
Effective length of database: 211,266,947
Effective search space: 94858859203
Effective search space used: 94858859203
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 45 (21.9 bits)