TBLASTN 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= SPP00000650_2.0 # Protein # Selenoprotein T (SelT) # Zebrafish
(Danio rerio)
         (210 letters)

Database: genome.fa 
           133,062 sequences; 684,497,465 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BADN01112526.1 Thunnus orientalis DNA, contig: superscaffoldBa00...   103   7e-33
BADN01025730.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    61   1e-09
BADN01025729.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    51   4e-06
BADN01112525.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    42   0.003

>BADN01112526.1 Thunnus orientalis DNA, contig: superscaffoldBa00008212_0002, whole
           genome shotgun sequence
          Length = 1914

 Score =  103 bits (258), Expect(2) = 7e-33,   Method: Compositional matrix adjust.
 Identities = 53/103 (51%), Positives = 66/103 (64%), Gaps = 23/103 (22%)
 Frame = +3

Query: 63  ISXGYSKVFQEYSRSISQLYPDIRIEGDNYPPKPIN-----------------------K 99
           IS GYSKVFQEYSR+ISQLYPDIRIEG+NYP  P+                        +
Sbjct: 36  IS*GYSKVFQEYSRAISQLYPDIRIEGENYP-HPLQQVSSDISNAPPGVL*HH*PPPPLR 212

Query: 100 YLGNFLSYFKLLAIALIVTGQNPFQMFGMNTPRIWAWGQENKI 142
            LGN +SY KL++I LIV+GQN F + G++TPR W W Q+NK+
Sbjct: 213 CLGNLISYLKLVSILLIVSGQNVFVLLGLDTPRAWTWSQDNKV 341



 Score = 58.5 bits (140), Expect(2) = 7e-33,   Method: Compositional matrix adjust.
 Identities = 25/30 (83%), Positives = 28/30 (93%)
 Frame = +1

Query: 141 KIFSCLMAFFISNMLETHFLSTGAFEITLN 170
           +IFSCLMAFF  NM+ETHFLSTGAFE+TLN
Sbjct: 406 QIFSCLMAFFFCNMMETHFLSTGAFEVTLN 495



 Score = 70.9 bits (172), Expect = 5e-13,   Method: Compositional matrix adjust.
 Identities = 28/40 (70%), Positives = 37/40 (92%)
 Frame = +2

Query: 168  TLNDIPIWSKLQSGYVPNIQELFQILDNHLKMNQADKMNF 207
            ++ D+P+WSKLQ+GYVPN+QE+FQILDNHLKMNQ D ++F
Sbjct: 1271 SVTDVPVWSKLQAGYVPNVQEIFQILDNHLKMNQVDPVSF 1390


>BADN01025730.1 Thunnus orientalis DNA, contig: superscaffoldBa00000534_0027, whole
            genome shotgun sequence
          Length = 5802

 Score = 61.2 bits (147), Expect = 1e-09,   Method: Compositional matrix adjust.
 Identities = 24/44 (54%), Positives = 33/44 (75%)
 Frame = -3

Query: 99   KYLGNFLSYFKLLAIALIVTGQNPFQMFGMNTPRIWAWGQENKI 142
            +++ +FLS FKLL I LI+ G++PF +FGM  P IW WGQ NK+
Sbjct: 1141 RHIASFLSVFKLLVIGLIIIGRDPFTLFGMQAPGIWEWGQGNKV 1010



 Score = 50.4 bits (119), Expect = 5e-06,   Method: Compositional matrix adjust.
 Identities = 21/37 (56%), Positives = 30/37 (81%)
 Frame = -2

Query: 63   ISXGYSKVFQEYSRSISQLYPDIRIEGDNYPPKPINK 99
            IS GY +VF+EY++++ Q YPDIRIEG+NY P P+ +
Sbjct: 4115 IS*GYKRVFEEYTQALYQRYPDIRIEGENYLPIPLYR 4005


>BADN01025729.1 Thunnus orientalis DNA, contig: superscaffoldBa00000534_0026, whole
            genome shotgun sequence
          Length = 6634

 Score = 50.8 bits (120), Expect = 4e-06,   Method: Compositional matrix adjust.
 Identities = 19/30 (63%), Positives = 28/30 (93%)
 Frame = -1

Query: 171  DIPIWSKLQSGYVPNIQELFQILDNHLKMN 200
            D+P+WSKL+SG++P++Q+L QILDN +KMN
Sbjct: 6319 DVPVWSKLESGHLPSMQQLVQILDNEMKMN 6230



 Score = 46.6 bits (109), Expect = 8e-05,   Method: Compositional matrix adjust.
 Identities = 19/30 (63%), Positives = 26/30 (86%)
 Frame = -1

Query: 141  KIFSCLMAFFISNMLETHFLSTGAFEITLN 170
            +I++C+M FF+SNM+E   +STGAFEITLN
Sbjct: 6628 QIYACMMVFFLSNMIENQLMSTGAFEITLN 6539


>BADN01112525.1 Thunnus orientalis DNA, contig: superscaffoldBa00008212_0001, whole
            genome shotgun sequence
          Length = 2606

 Score = 42.4 bits (98), Expect = 0.003,   Method: Compositional matrix adjust.
 Identities = 23/61 (37%), Positives = 33/61 (54%)
 Frame = +3

Query: 1    MAEYSQTGIXXXXXXXXXXXXKDIYVGRNSVTQQENTGPDINTQRQNKHTFYTGPVLKFQ 60
            MAEYSQ G+            +D+Y+GR  ++   +  PD    +  K + YTGPVL+FQ
Sbjct: 1992 MAEYSQAGLLAALLLFTALTVRDLYLGRPGLS--PDNLPDTEPGKPAKPSLYTGPVLRFQ 2165

Query: 61   Y 61
            Y
Sbjct: 2166 Y 2168


  Database: genome.fa
    Posted date:  Oct 9, 2014  7:00 PM
  Number of letters in database: 684,497,465
  Number of sequences in database:  133,062
  
Lambda     K      H
   0.321    0.137    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 133062
Number of Hits to DB: 165,945,031
Number of extensions: 2526350
Number of successful extensions: 10305
Number of sequences better than 1.0e-02: 4
Number of HSP's gapped: 10301
Number of HSP's successfully gapped: 8
Length of query: 210
Length of database: 228,165,821
Length adjustment: 116
Effective length of query: 94
Effective length of database: 212,730,629
Effective search space: 19996679126
Effective search space used: 19996679126
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 41 (20.4 bits)