TBLASTN 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= SPP00000648_2.0 # Protein # Selenoprotein S (SelS) # Zebrafish
(Danio rerio)
         (190 letters)

Database: genome.fa 
           133,062 sequences; 684,497,465 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BADN01048450.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    50   2e-14

>BADN01048450.1 Thunnus orientalis DNA, contig: superscaffoldBa00001412_0016, whole
            genome shotgun sequence
          Length = 11117

 Score = 50.1 bits (118), Expect(2) = 2e-14,   Method: Compositional matrix adjust.
 Identities = 22/29 (75%), Positives = 28/29 (96%)
 Frame = -1

Query: 110  EEEKRRQKIEMWDSMQEGKSYKGSAKVAQ 138
            E+EKRRQKIE+W+SMQ+GKSYKG+ KV+Q
Sbjct: 1496 EQEKRRQKIEIWESMQQGKSYKGATKVSQ 1410



 Score = 49.7 bits (117), Expect(2) = 2e-14,   Method: Compositional matrix adjust.
 Identities = 24/34 (70%), Positives = 28/34 (82%)
 Frame = -3

Query: 73   DVGSVVRRQEALEASRRRMQEEQDARAAEFREKQ 106
            D   V RRQEA+EA+RR+MQEE DA+AA FREKQ
Sbjct: 1701 DAVLVARRQEAMEAARRKMQEELDAKAALFREKQ 1600


  Database: genome.fa
    Posted date:  Oct 9, 2014  7:00 PM
  Number of letters in database: 684,497,465
  Number of sequences in database:  133,062
  
Lambda     K      H
   0.312    0.126    0.358 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 133062
Number of Hits to DB: 97,605,283
Number of extensions: 979088
Number of successful extensions: 4933
Number of sequences better than 1.0e-02: 3
Number of HSP's gapped: 4904
Number of HSP's successfully gapped: 4
Length of query: 190
Length of database: 228,165,821
Length adjustment: 114
Effective length of query: 76
Effective length of database: 212,996,753
Effective search space: 16187753228
Effective search space used: 16187753228
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 41 (20.4 bits)