TBLASTN 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= SPP00000644_2.0 # Protein # Selenoprotein R  (SelR) # Zebrafish
(Danio rerio)
         (104 letters)

Database: genome.fa 
           133,062 sequences; 684,497,465 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BADN01013935.1 Thunnus orientalis DNA, contig: superscaffoldBa00...   101   5e-25
BADN01040629.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    94   2e-22

>BADN01013935.1 Thunnus orientalis DNA, contig: superscaffoldBa00000232_0021, whole
           genome shotgun sequence
          Length = 10275

 Score =  101 bits (251), Expect = 5e-25,   Method: Compositional matrix adjust.
 Identities = 62/144 (43%), Positives = 72/144 (50%), Gaps = 55/144 (38%)
 Frame = +2

Query: 16  FKPGIYACSQCGHPLFSSRSKYEHSSPWPAFTETI------------------------- 50
           +  G+Y CS+C H LFSSRSKYEHSSPWPAFTETI                         
Sbjct: 725 YSSGMYICSKCDHQLFSSRSKYEHSSPWPAFTETIHEESVSKHEERPGAYKVPSEEKRFT 904

Query: 51  -------REDSV------------------TKMMETLTA-----YKVLCGKCGNGLGHEF 80
                  R  SV                  T++ + +       ++V CGKCGNGLGHEF
Sbjct: 905 FLIIESFRGSSV*TYYIHF*TSHHTLSYLNTQLKQLIVVCVLFLFQVRCGKCGNGLGHEF 1084

Query: 81  LNDGPEEGLSRFXIFSHSLKFVPK 104
           +NDGP  G SRF IFS SLKFVPK
Sbjct: 1085VNDGPGRGQSRF*IFSSSLKFVPK 1156



 Score = 75.1 bits (183), Expect = 6e-16,   Method: Compositional matrix adjust.
 Identities = 38/64 (59%), Positives = 45/64 (70%)
 Frame = +2

Query: 4   CSFFGKEFYKDHFKPGIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTKMMETLT 63
           C+F  +      +  G+Y CS+C H LFSSRSKYEHSSPWPAFTETI E+SV+K  E   
Sbjct: 689 CTF*HQPLSPILYSSGMYICSKCDHQLFSSRSKYEHSSPWPAFTETIHEESVSKHEERPG 868

Query: 64  AYKV 67
           AYKV
Sbjct: 869 AYKV 880



 Score = 70.1 bits (170), Expect = 4e-14,   Method: Compositional matrix adjust.
 Identities = 33/49 (67%), Positives = 38/49 (77%)
 Frame = +3

Query: 19   GIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTKMMETLTAYKV 67
            GIY CS+CGH LFSS SK+EHSSPWPAF+ETI +DSV+K  E     KV
Sbjct: 3195 GIYVCSECGHKLFSSLSKFEHSSPWPAFSETIHKDSVSKHPEAWGPIKV 3341



 Score = 68.9 bits (167), Expect = 1e-13,   Method: Compositional matrix adjust.
 Identities = 32/39 (82%), Positives = 34/39 (87%)
 Frame = +2

Query: 66   KVLCGKCGNGLGHEFLNDGPEEGLSRFXIFSHSLKFVPK 104
            +V CGKCGNGLGHEFLNDGP EGLSRF IFS SL F+PK
Sbjct: 3491 QVCCGKCGNGLGHEFLNDGPREGLSRF*IFSSSLTFIPK 3607


>BADN01040629.1 Thunnus orientalis DNA, contig: superscaffoldBa00001062_0012, whole
             genome shotgun sequence
          Length = 19958

 Score = 94.0 bits (232), Expect = 2e-22,   Method: Compositional matrix adjust.
 Identities = 42/52 (80%), Positives = 46/52 (88%)
 Frame = -3

Query: 16    FKPGIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTKMMETLTAYKV 67
             F  G+Y CS+C HPLFSSRSK+ HSSPWPAFT TIREDSVTKMMETLTA+KV
Sbjct: 13524 FPAGMYVCSKCNHPLFSSRSKFAHSSPWPAFTNTIREDSVTKMMETLTAFKV 13369



 Score = 74.7 bits (182), Expect = 1e-15,   Method: Compositional matrix adjust.
 Identities = 36/52 (69%), Positives = 40/52 (76%)
 Frame = -1

Query: 53    DSVTKMMETLTAYKVLCGKCGNGLGHEFLNDGPEEGLSRFXIFSHSLKFVPK 104
             D+ T         +VLCGKCGNGLGHEF+NDGPEEG+SRF IFSHSLKFVP 
Sbjct: 13283 DTGTNTFILSLHTQVLCGKCGNGLGHEFVNDGPEEGVSRF*IFSHSLKFVPN 13128


  Database: genome.fa
    Posted date:  Oct 9, 2014  7:00 PM
  Number of letters in database: 684,497,465
  Number of sequences in database:  133,062
  
Lambda     K      H
   0.323    0.138    0.452 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 133062
Number of Hits to DB: 111,920,300
Number of extensions: 1694497
Number of successful extensions: 7042
Number of sequences better than 1.0e-02: 2
Number of HSP's gapped: 7042
Number of HSP's successfully gapped: 7
Length of query: 104
Length of database: 228,165,821
Length adjustment: 78
Effective length of query: 26
Effective length of database: 217,786,985
Effective search space: 5662461610
Effective search space used: 5662461610
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 38 (19.2 bits)