TBLASTN 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= SPP00000647_2.0 # Protein # Methionine-R-sufoxide reductase 3
(SelR3) # Zebrafish (Danio rerio)
         (186 letters)

Database: genome.fa 
           133,062 sequences; 684,497,465 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BADN01030477.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    77   2e-15
BADN01055509.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    58   8e-09
BADN01030478.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    44   4e-04
BADN01013935.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    40   0.008

>BADN01030477.1 Thunnus orientalis DNA, contig: superscaffoldBa00000683_0018, whole
            genome shotgun sequence
          Length = 14015

 Score = 77.4 bits (189), Expect = 2e-15,   Method: Compositional matrix adjust.
 Identities = 38/59 (64%), Positives = 44/59 (74%), Gaps = 2/59 (3%)
 Frame = -1

Query: 130  QCGAHLGHLFDDGPRPTKKRYCINSASLSFQPKNSATEEVE--ASGAAGQSSTSKNEEL 186
            QCGAHLGHLFDDGPRPT KRYCINSASL+FQPK++A+      A G A  S + +  EL
Sbjct: 6632 QCGAHLGHLFDDGPRPTGKRYCINSASLAFQPKDTASSISSSTAEGEAVSSMSDRKTEL 6456



 Score = 65.5 bits (158), Expect = 3e-11,   Method: Compositional matrix adjust.
 Identities = 28/38 (73%), Positives = 34/38 (89%)
 Frame = -3

Query: 24    PSGTCRSKKTWPKTFPEEELRKRLTPMQYHVTQQKGTE 61
             PSG  R+KKTWP +F +EE+RKRLTPMQYHVTQ++GTE
Sbjct: 13602 PSGARRTKKTWPVSFSQEEIRKRLTPMQYHVTQERGTE 13489



 Score = 62.8 bits (151), Expect = 2e-10,   Method: Compositional matrix adjust.
 Identities = 25/33 (75%), Positives = 30/33 (90%)
 Frame = -2

Query: 98   GWPSFFDLINKDAIAQTDDFSYGMHRVETTCSQ 130
            GWPSFFDL+ +++I  +DDFSYGMHRVETTCSQ
Sbjct: 8302 GWPSFFDLMKEESITLSDDFSYGMHRVETTCSQ 8204



 Score = 48.1 bits (113), Expect = 2e-05,   Method: Compositional matrix adjust.
 Identities = 20/34 (58%), Positives = 25/34 (73%)
 Frame = -1

Query: 54    VTQQKGTESAFTGKYVDNKEEGTYHCVVCGAPLF 87
             +T      SAF G++ D+K+EGTY CVVCGAPLF
Sbjct: 13208 ITNTVFFHSAFAGEFTDHKDEGTYACVVCGAPLF 13107


>BADN01055509.1 Thunnus orientalis DNA, contig: superscaffoldBa00001764_0008, whole
             genome shotgun sequence
          Length = 16368

 Score = 58.2 bits (139), Expect = 8e-09,   Method: Compositional matrix adjust.
 Identities = 22/39 (56%), Positives = 30/39 (76%)
 Frame = +1

Query: 130   QCGAHLGHLFDDGPRPTKKRYCINSASLSFQPKNSATEE 168
             QC +HLGH+FDDGP PT +R+CINS +L+F+P+     E
Sbjct: 11617 QCDSHLGHVFDDGPDPTGQRFCINSVALTFKPREDNNSE 11733


>BADN01030478.1 Thunnus orientalis DNA, contig: superscaffoldBa00000683_0019, whole
            genome shotgun sequence
          Length = 15790

 Score = 44.3 bits (103), Expect = 4e-04,   Method: Compositional matrix adjust.
 Identities = 18/26 (69%), Positives = 23/26 (88%)
 Frame = -3

Query: 1    MSGFNLLHLVSQGQPVKLKACGLPSG 26
            MSGFNLLHLV++ QPV L++C LP+G
Sbjct: 2510 MSGFNLLHLVTKSQPVTLRSCSLPTG 2433


>BADN01013935.1 Thunnus orientalis DNA, contig: superscaffoldBa00000232_0021, whole
            genome shotgun sequence
          Length = 10275

 Score = 40.4 bits (93), Expect = 0.008,   Method: Compositional matrix adjust.
 Identities = 20/54 (37%), Positives = 32/54 (59%)
 Frame = +3

Query: 75   GTYHCVVCGAPLFMSEKKFDSGSGWPSFFDLINKDAIAQTDDFSYGMHRVETTC 128
            G Y C  CG  LF S  KF+  S WP+F + I+KD++++  + ++G  +V   C
Sbjct: 3195 GIYVCSECGHKLFSSLSKFEHSSPWPAFSETIHKDSVSKHPE-AWGPIKVNYHC 3353


  Database: genome.fa
    Posted date:  Oct 9, 2014  7:00 PM
  Number of letters in database: 684,497,465
  Number of sequences in database:  133,062
  
Lambda     K      H
   0.314    0.130    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 133062
Number of Hits to DB: 168,588,189
Number of extensions: 2538212
Number of successful extensions: 7640
Number of sequences better than 1.0e-02: 4
Number of HSP's gapped: 7638
Number of HSP's successfully gapped: 7
Length of query: 186
Length of database: 228,165,821
Length adjustment: 114
Effective length of query: 72
Effective length of database: 212,996,753
Effective search space: 15335766216
Effective search space used: 15335766216
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 41 (20.4 bits)