TBLASTN 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= tr|E9QHL7|E9QHL7_DANRE Uncharacterized protein OS=Danio rerio
GN=msrb1a PE=4 SV=1
         (148 letters)

Database: genome.fa 
           133,062 sequences; 684,497,465 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BADN01013935.1 Thunnus orientalis DNA, contig: superscaffoldBa00...   119   1e-30
BADN01040629.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    80   4e-17

>BADN01013935.1 Thunnus orientalis DNA, contig: superscaffoldBa00000232_0021, whole
           genome shotgun sequence
          Length = 10275

 Score =  119 bits (298), Expect = 1e-30,   Method: Compositional matrix adjust.
 Identities = 68/144 (47%), Positives = 81/144 (56%), Gaps = 55/144 (38%)
 Frame = +2

Query: 16  FESGMYVCAQCGYELFSSRSKYEHSSPWPAFTETIHEDSVSK------------QEERWG 63
           + SGMY+C++C ++LFSSRSKYEHSSPWPAFTETIHE+SVSK            +E+R+ 
Sbjct: 725 YSSGMYICSKCDHQLFSSRSKYEHSSPWPAFTETIHEESVSKHEERPGAYKVPSEEKRFT 904

Query: 64  -------------------------------------------AYKVRCGKCGNGLGHEF 80
                                                       ++VRCGKCGNGLGHEF
Sbjct: 905 FLIIESFRGSSV*TYYIHF*TSHHTLSYLNTQLKQLIVVCVLFLFQVRCGKCGNGLGHEF 1084

Query: 81  VNDGPKHGLSRFCIFSSSLKFIPK 104
           VNDGP  G SRF IFSSSLKF+PK
Sbjct: 1085VNDGPGRGQSRF*IFSSSLKFVPK 1156



 Score = 90.1 bits (222), Expect = 2e-20,   Method: Compositional matrix adjust.
 Identities = 43/64 (67%), Positives = 52/64 (81%)
 Frame = +2

Query: 4   CSFSGGEIYKDHFESGMYVCAQCGYELFSSRSKYEHSSPWPAFTETIHEDSVSKQEERWG 63
           C+F    +    + SGMY+C++C ++LFSSRSKYEHSSPWPAFTETIHE+SVSK EER G
Sbjct: 689 CTF*HQPLSPILYSSGMYICSKCDHQLFSSRSKYEHSSPWPAFTETIHEESVSKHEERPG 868

Query: 64  AYKV 67
           AYKV
Sbjct: 869 AYKV 880



 Score = 81.3 bits (199), Expect = 2e-17,   Method: Compositional matrix adjust.
 Identities = 36/51 (70%), Positives = 44/51 (86%)
 Frame = +3

Query: 18   SGMYVCAQCGYELFSSRSKYEHSSPWPAFTETIHEDSVSKQEERWGAYKVR 68
            SG+YVC++CG++LFSS SK+EHSSPWPAF+ETIH+DSVSK  E WG  KV 
Sbjct: 3192 SGIYVCSECGHKLFSSLSKFEHSSPWPAFSETIHKDSVSKHPEAWGPIKVN 3344



 Score = 67.8 bits (164), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 32/39 (82%), Positives = 35/39 (89%)
 Frame = +2

Query: 66   KVRCGKCGNGLGHEFVNDGPKHGLSRFCIFSSSLKFIPK 104
            +V CGKCGNGLGHEF+NDGP+ GLSRF IFSSSL FIPK
Sbjct: 3491 QVCCGKCGNGLGHEFLNDGPREGLSRF*IFSSSLTFIPK 3607


>BADN01040629.1 Thunnus orientalis DNA, contig: superscaffoldBa00001062_0012, whole
             genome shotgun sequence
          Length = 19958

 Score = 80.5 bits (197), Expect = 4e-17,   Method: Composition-based stats.
 Identities = 35/53 (66%), Positives = 42/53 (79%)
 Frame = -3

Query: 16    FESGMYVCAQCGYELFSSRSKYEHSSPWPAFTETIHEDSVSKQEERWGAYKVR 68
             F +GMYVC++C + LFSSRSK+ HSSPWPAFT TI EDSV+K  E   A+KVR
Sbjct: 13524 FPAGMYVCSKCNHPLFSSRSKFAHSSPWPAFTNTIREDSVTKMMETLTAFKVR 13366



 Score = 66.6 bits (161), Expect = 3e-12,   Method: Compositional matrix adjust.
 Identities = 30/40 (75%), Positives = 35/40 (87%)
 Frame = -1

Query: 66    KVRCGKCGNGLGHEFVNDGPKHGLSRFCIFSSSLKFIPKE 105
             +V CGKCGNGLGHEFVNDGP+ G+SRF IFS SLKF+P +
Sbjct: 13244 QVLCGKCGNGLGHEFVNDGPEEGVSRF*IFSHSLKFVPNK 13125


  Database: genome.fa
    Posted date:  Oct 9, 2014  7:00 PM
  Number of letters in database: 684,497,465
  Number of sequences in database:  133,062
  
Lambda     K      H
   0.320    0.136    0.452 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 133062
Number of Hits to DB: 180,652,006
Number of extensions: 3430519
Number of successful extensions: 13906
Number of sequences better than 1.0e-02: 2
Number of HSP's gapped: 13905
Number of HSP's successfully gapped: 6
Length of query: 148
Length of database: 228,165,821
Length adjustment: 109
Effective length of query: 39
Effective length of database: 213,662,063
Effective search space: 8332820457
Effective search space used: 8332820457
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 40 (20.0 bits)