TBLASTN 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= SPP00000642_2.0 # Protein # Selenoprotein P (SelP) # Zebrafish
(Danio rerio)
         (327 letters)

Database: genome.fa 
           133,062 sequences; 684,497,465 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BADN01078433.1 Thunnus orientalis DNA, contig: superscaffoldBa00...   109   5e-25
BADN01078432.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    84   2e-16
BADN01026358.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    74   4e-13

>BADN01078433.1 Thunnus orientalis DNA, contig: superscaffoldBa00003326_0002, whole
           genome shotgun sequence
          Length = 9594

 Score =  109 bits (273), Expect = 5e-25,   Method: Compositional matrix adjust.
 Identities = 47/71 (66%), Positives = 60/71 (84%)
 Frame = +2

Query: 74  LGDLRDKLANGGLTNISFMVVNEQDSQSRAMYWELKRRTAQDIPVYQQSPLQNDVWEILE 133
           +G LRD+L    LT++SFM+VNE+++ SRAMYWELKR+   D+PVYQQ+P Q+DVWE L+
Sbjct: 800 IGGLRDRLNRSKLTDVSFMIVNEREALSRAMYWELKRKAPPDVPVYQQAPFQSDVWEELD 979

Query: 134 GDKDDFLVYDR 144
           GDKDDFLVYDR
Sbjct: 980 GDKDDFLVYDR 1012



 Score = 79.3 bits (194), Expect = 1e-14,   Method: Compositional matrix adjust.
 Identities = 32/41 (78%), Positives = 40/41 (97%)
 Frame = +1

Query: 144  RCGYLTFHIVLPFSFLHYPYIEAAIRATYHKNMCNCSLNAN 184
            RCG LT+H+VLP+SFLHYPY+EAAIRATYHKN+CNCS+++N
Sbjct: 1531 RCGLLTYHVVLPYSFLHYPYVEAAIRATYHKNICNCSVSSN 1653


>BADN01078432.1 Thunnus orientalis DNA, contig: superscaffoldBa00003326_0001, whole
             genome shotgun sequence
          Length = 11853

 Score = 84.3 bits (207), Expect = 2e-16,   Method: Compositional matrix adjust.
 Identities = 45/75 (60%), Positives = 56/75 (74%), Gaps = 2/75 (2%)
 Frame = +2

Query: 1     MQALWPLLLSAFPALLGAS--SLFVEKESSGSRICKPAPQWEIDGKTPMKELLGNVVVVA 58
             + +LW  L +A P LL AS  SL VE  ++ SRICKPAP W I G+ PM++LLG+VVVVA
Sbjct: 11345 LSSLW--LCAALPGLLCASQVSLLVEGGNNASRICKPAPNWTIKGQAPMQKLLGSVVVVA 11518

Query: 59    LLKASXHFCLTQAAR 73
             LLKAS  FCLTQA++
Sbjct: 11519 LLKAS*QFCLTQASK 11563


>BADN01026358.1 Thunnus orientalis DNA, contig: superscaffoldBa00000553_0019, whole
            genome shotgun sequence
          Length = 20372

 Score = 74.3 bits (181), Expect = 4e-13,   Method: Compositional matrix adjust.
 Identities = 37/99 (37%), Positives = 60/99 (60%)
 Frame = -2

Query: 73   RLGDLRDKLANGGLTNISFMVVNEQDSQSRAMYWELKRRTAQDIPVYQQSPLQNDVWEIL 132
            R+  LR KL   GL ++ +MV+N Q +Q++ ++  L++  ++++ +Y+Q   Q DVW  L
Sbjct: 7984 RMDSLRQKLERRGLKDVIYMVINHQGAQAQKLHSMLEQSLSENVTLYKQLEHQPDVWRTL 7805

Query: 133  EGDKDDFLVYDRCGYLTFHIVLPFSFLHYPYIEAAIRAT 171
             G+KDDFL+YDR      HI   FSF+ + YI  A   +
Sbjct: 7804 NGEKDDFLIYDRFVTCIQHI---FSFVLFLYIRFAFNTS 7697



 Score = 46.2 bits (108), Expect = 4e-04,   Method: Compositional matrix adjust.
 Identities = 21/41 (51%), Positives = 26/41 (63%)
 Frame = -3

Query: 33   CKPAPQWEIDGKTPMKELLGNVVVVALLKASXHFCLTQAAR 73
            C+  P W+I    PM E +G V VVAL +AS  FCL QA+R
Sbjct: 8508 CQQPPAWKIGEVEPMTEAIGRVTVVALFQAS*QFCLVQASR 8386


  Database: genome.fa
    Posted date:  Oct 9, 2014  7:00 PM
  Number of letters in database: 684,497,465
  Number of sequences in database:  133,062
  
Lambda     K      H
   0.319    0.133    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 133062
Number of Hits to DB: 298,368,382
Number of extensions: 4868414
Number of successful extensions: 32023
Number of sequences better than 1.0e-02: 3
Number of HSP's gapped: 31177
Number of HSP's successfully gapped: 5
Length of query: 327
Length of database: 228,165,821
Length adjustment: 121
Effective length of query: 206
Effective length of database: 212,065,319
Effective search space: 43685455714
Effective search space used: 43685455714
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 43 (21.2 bits)