TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000138_2.0 # Protein # Thioredoxin reductase 2 (TR2) #
Human (Homo sapiens)
(524 letters)
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g
enome.fa
14,644 sequences; 2,174,243,242 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|397458791|gb|JH736710.1| Alligator mississippiensis unplaced ... 105 5e-21
gi|397461694|gb|JH733807.1| Alligator mississippiensis unplaced ... 96 1e-20
gi|397459659|gb|JH735842.1| Alligator mississippiensis unplaced ... 93 4e-17
gi|397461104|gb|JH734397.1| Alligator mississippiensis unplaced ... 52 9e-05
gi|397460178|gb|JH735323.1| Alligator mississippiensis unplaced ... 42 0.056
gi|397461636|gb|JH733865.1| Alligator mississippiensis unplaced ... 35 6.8
gi|397464324|gb|JH731177.1| Alligator mississippiensis unplaced ... 35 6.8
>gi|397458791|gb|JH736710.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-9279, whole genome shotgun sequence
Length = 567381
Score = 105 bits (262), Expect = 5e-21
Identities = 51/80 (63%), Positives = 62/80 (77%)
Frame = -1
Query: 208 SDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEH 267
SDD+F L PGKTLVVGASYVALECAGFL GIGLD T+M+RSI LRGFDQ+M++ + EH
Sbjct: 446484 SDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQEMANKIGEH 446305
Query: 268 MASHGTRFLRGCAPSRVRRL 287
M HG +F++ P +V L
Sbjct: 446304 MEEHGIKFIKQFVPIKVSLL 446245
Score = 73.2 bits (178), Expect = 3e-11
Identities = 40/107 (37%), Positives = 51/107 (47%), Gaps = 43/107 (40%)
Frame = -2
Query: 56 KEAAQLGRKVAVVDYVEPSPQGTRWG---------------------------------- 81
+EA + +KV V+DYV P+P GT WG
Sbjct: 451478 QEAVKYDKKVMVLDYVTPTPLGTSWGNFLLITDIL*RGISYYMALQFYFGVVFIDT*NIL 451299
Query: 82 ---------LGGTCVNVGCIPKKLMHQAALLGGLIQDAPNYGWEVAQ 119
LGGTCVNVGCIPKKLMHQAALLG ++D+ +GW+ +
Sbjct: 451298 CLLSLLPQGLGGTCVNVGCIPKKLMHQAALLGQALKDSHKFGWQFTE 451158
Score = 63.2 bits (152), Expect = 3e-08
Identities = 26/43 (60%), Positives = 33/43 (76%)
Frame = -2
Query: 460 VLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSE 502
++G H LGPNAGEVTQGFA IKCG + ++ T+GIHP C+E
Sbjct: 433097 IIGFHVLGPNAGEVTQGFAAAIKCGLTKEKLDSTIGIHPVCAE 432969
Score = 60.5 bits (145), Expect = 2e-07
Identities = 27/51 (52%), Positives = 32/51 (62%)
Frame = -2
Query: 117 VAQPVPHDWRKMAEAVQNHVKSLNWGHRVQLQDRKVKYFNIKASFVDEHTV 167
V V H W M EAVQN++ SLNWG+RV L+D KV Y N FV HT+
Sbjct: 449156 VFSAVKHSWETMTEAVQNYIGSLNWGYRVALRDNKVTYENAYGEFVGPHTI 449004
Score = 60.5 bits (145), Expect = 2e-07
Identities = 28/48 (58%), Positives = 38/48 (79%)
Frame = -2
Query: 340 KILVDSREATSVPHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSS 387
KI + E T+VP+IYAIGDV+E + ELTP+AI AGRLL +RL+ G++
Sbjct: 440171 KIPANDEEQTNVPYIYAIGDVLEDKLELTPVAIQAGRLLTRRLYAGAT 440028
Score = 52.4 bits (124), Expect = 5e-05
Identities = 22/36 (61%), Positives = 28/36 (77%)
Frame = -3
Query: 391 DYDNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEV 426
DY NVPTTVFTP+EYG G SEE A+ + G+ ++EV
Sbjct: 436735 DYVNVPTTVFTPMEYGACGFSEERALEKFGENNIEV 436628
Score = 42.0 bits (97), Expect = 0.073
Identities = 15/28 (53%), Positives = 23/28 (82%)
Frame = -2
Query: 425 EVYHAHYKPLEFTVAGRDASQCYVKMVC 452
+VYH+ + PLE+TV GRD ++CY K++C
Sbjct: 434801 QVYHSFFWPLEWTVPGRDNNKCYAKIIC 434718
>gi|397461694|gb|JH733807.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-4487, whole genome shotgun sequence
Length = 316803
Score = 95.5 bits (236), Expect(2) = 1e-20
Identities = 46/74 (62%), Positives = 56/74 (75%)
Frame = -2
Query: 208 SDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEH 267
SDD+ L PGKTLVVGAS VALECAGFL GIGLD ++M+RSI LRGFDQ+M+ + H
Sbjct: 47732 SDDLXSLPYCPGKTLVVGASXVALECAGFLAGIGLDVSVMVRSILLRGFDQEMAERIGAH 47553
Query: 268 MASHGTRFLRGCAP 281
M +HG +F+R P
Sbjct: 47552 METHGVKFIRRFVP 47511
Score = 68.9 bits (167), Expect = 6e-10
Identities = 31/56 (55%), Positives = 39/56 (69%)
Frame = -3
Query: 460 VLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVKLRISKRSGL 515
V+G H LGPNAGE+TQGFA IKCG + + T+GIHPTC+E KL + R+ L
Sbjct: 41782 VIGFHVLGPNAGEITQGFAAAIKCGLTKELLNETIGIHPTCAEVRGKLELQGRNSL 41615
Score = 65.1 bits (157), Expect = 8e-09
Identities = 27/36 (75%), Positives = 32/36 (88%)
Frame = -3
Query: 81 GLGGTCVNVGCIPKKLMHQAALLGGLIQDAPNYGWE 116
GLGGTCVNVGCIPKKLMHQAALLG ++D+ +GW+
Sbjct: 52402 GLGGTCVNVGCIPKKLMHQAALLGQALKDSRKFGWQ 52295
Score = 64.7 bits (156), Expect = 1e-08
Identities = 29/48 (60%), Positives = 39/48 (81%)
Frame = -1
Query: 340 KILVDSREATSVPHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSS 387
KI V E T+VP++YAIGD+++G+ ELTP+AI AGRLL +RL+ GSS
Sbjct: 44907 KIPVSDEEQTNVPYVYAIGDILDGKLELTPVAIQAGRLLARRLYAGSS 44764
Score = 59.7 bits (143), Expect = 3e-07
Identities = 24/51 (47%), Positives = 35/51 (68%)
Frame = -2
Query: 117 VAQPVPHDWRKMAEAVQNHVKSLNWGHRVQLQDRKVKYFNIKASFVDEHTV 167
V+ V H+W M EA+QN++ SLNWG+RV L+++ V Y N A F++ H V
Sbjct: 50783 VSVSVKHNWETMVEAIQNYIGSLNWGYRVSLREKTVTYLNAYAEFIEPHKV 50631
Score = 57.8 bits (138), Expect = 1e-06
Identities = 26/41 (63%), Positives = 32/41 (78%)
Frame = -1
Query: 386 SSDLMDYDNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEV 426
SS DY NVPTTVFTPLEYGC GL EE+A+ +G+ ++EV
Sbjct: 44508 SSFQCDYVNVPTTVFTPLEYGCCGLPEEKAIKEYGEHNLEV 44386
Score = 43.5 bits (101), Expect = 0.025
Identities = 21/42 (50%), Positives = 28/42 (66%), Gaps = 1/42 (2%)
Frame = -3
Query: 56 KEAAQLGRKVAVVDYVEPSPQGTRWGLGG-TCVNVGCIPKKL 96
+EAA LG+KV V+DYV P+P GT WG C+ + C+ K L
Sbjct: 53647 QEAATLGKKVMVLDYVVPTPSGTSWGKAQIICLELKCM*KDL 53522
Score = 37.0 bits (84), Expect = 2.3
Identities = 14/27 (51%), Positives = 19/27 (70%)
Frame = -1
Query: 426 VYHAHYKPLEFTVAGRDASQCYVKMVC 452
VYH + PLE+TV RD + CY K++C
Sbjct: 43080 VYHTLFWPLEWTVPNRDNNTCYAKIIC 43000
Score = 29.6 bits (65), Expect(2) = 1e-20
Identities = 17/37 (45%), Positives = 22/37 (59%)
Frame = -3
Query: 174 GKEILLSADHIIIATGGRPRYPTHIEGALEYGITSDD 210
G+E +A+ ++ATG RPRY I G EY IT D
Sbjct: 47953 GQETYHTAERFVLATGERPRY-LGIPGDKEYCITR*D 47846
>gi|397459659|gb|JH735842.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-7838, whole genome shotgun sequence
Length = 1444372
Score = 92.8 bits (229), Expect = 4e-17
Identities = 38/51 (74%), Positives = 44/51 (86%)
Frame = +1
Query: 77 GTRWGLGGTCVNVGCIPKKLMHQAALLGGLIQDAPNYGWEVAQPVPHDWRK 127
GT WGLGGTCVNVGCIPKKLMHQAALLG ++DA +YGW ++QP+ HDW K
Sbjct: 864322 GTSWGLGGTCVNVGCIPKKLMHQAALLGSALKDARHYGWNISQPIHHDW*K 864474
Score = 82.0 bits (201), Expect = 6e-14
Identities = 33/61 (54%), Positives = 50/61 (81%)
Frame = +1
Query: 259 QMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIGR 318
QMSS+V ++M SHGTRFL+ C P++V L G+LQV+W+++ GKE+T +FDTV+WA+G+
Sbjct: 876133 QMSSLVADYMESHGTRFLKKCIPTKVEELDSGKLQVSWKNADVGKEETDSFDTVIWAVGK 876312
Query: 319 V 319
+
Sbjct: 876313 L 876315
Score = 70.1 bits (170), Expect = 2e-10
Identities = 31/43 (72%), Positives = 38/43 (88%)
Frame = +3
Query: 482 KCGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCXG 524
+CGA+Y+Q+M+TVGIHPT +EEV KL I+KRSGLD TVTGC G
Sbjct: 898968 RCGATYSQMMKTVGIHPTSAEEVTKLHITKRSGLDATVTGC*G 899096
Score = 69.7 bits (169), Expect = 3e-10
Identities = 33/52 (63%), Positives = 41/52 (78%)
Frame = +1
Query: 311 TVLWAIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREATSVPHIYAIGDVVE 362
T L+ GRVPDTR+LNLE GV + +T KI+VD+ EATSVPHIYA+GD+ E
Sbjct: 886942 TSLFP*GRVPDTRTLNLETVGVKMNSETGKIIVDASEATSVPHIYAVGDITE 887097
Score = 60.5 bits (145), Expect = 2e-07
Identities = 27/30 (90%), Positives = 30/30 (100%)
Frame = +3
Query: 229 VALECAGFLTGIGLDTTIMMRSIPLRGFDQ 258
V+LECAGFLTGIGLDTT+M+RSIPLRGFDQ
Sbjct: 873474 VSLECAGFLTGIGLDTTVMVRSIPLRGFDQ 873563
Score = 60.1 bits (144), Expect = 3e-07
Identities = 29/40 (72%), Positives = 33/40 (82%)
Frame = +2
Query: 443 ASQCYVKMVCLREPPQLVLGLHFLGPNAGEVTQGFALGIK 482
A C+ +MVCLRE Q +LGLHF+GPNAGEV QGFALGIK
Sbjct: 896738 AVNCF-QMVCLREKEQRLLGLHFIGPNAGEVIQGFALGIK 896854
Score = 57.8 bits (138), Expect = 1e-06
Identities = 25/32 (78%), Positives = 30/32 (93%)
Frame = +2
Query: 395 VPTTVFTPLEYGCVGLSEEEAVARHGQEHVEV 426
VPTTVFTPLEYGCVGLSEE+AV R+G+++ EV
Sbjct: 893261 VPTTVFTPLEYGCVGLSEEKAVGRYGEDNTEV 893356
Score = 52.4 bits (124), Expect = 5e-05
Identities = 24/34 (70%), Positives = 29/34 (85%)
Frame = +1
Query: 362 EGRPELTPIAIMAGRLLVQRLFGGSSDLMDYDNV 395
+GRPELTP AI AG+LL +RLF SS+LMDYD+V
Sbjct: 892882 QGRPELTPTAIAAGKLLARRLFSQSSELMDYDSV 892983
Score = 50.4 bits (119), Expect = 2e-04
Identities = 21/29 (72%), Positives = 26/29 (89%)
Frame = +3
Query: 425 EVYHAHYKPLEFTVAGRDASQCYVKMVCL 453
+VYHA+YKPLEFTVA RDA+QCY+K+ L
Sbjct: 894891 QVYHAYYKPLEFTVAERDATQCYIKVSVL 894977
Score = 47.8 bits (112), Expect = 0.001
Identities = 19/24 (79%), Positives = 23/24 (95%)
Frame = +2
Query: 198 IEGALEYGITSDDIFWLKESPGKT 221
+ GAL+YGITSDD+FWLK+SPGKT
Sbjct: 867851 VAGALQYGITSDDLFWLKKSPGKT 867922
Score = 47.4 bits (111), Expect = 0.002
Identities = 20/23 (86%), Positives = 23/23 (100%)
Frame = +2
Query: 128 MAEAVQNHVKSLNWGHRVQLQDR 150
MA+AVQN+VKSLNWGHRVQLQD+
Sbjct: 864812 MAQAVQNYVKSLNWGHRVQLQDK 864880
Score = 46.6 bits (109), Expect = 0.003
Identities = 22/50 (44%), Positives = 29/50 (58%)
Frame = +2
Query: 149 DRKVKYFNIKASFVDEHTVCGVAKGGKEILLSADHIIIATGGRPRYPTHI 198
+RKVKYFN+K SF D HT+ G+ K GKE+ A + R PT +
Sbjct: 865955 NRKVKYFNLKGSFADAHTIRGLTKAGKEVSCLAGYYSTWFDARNSRPTSV 866104
Score = 38.1 bits (87), Expect = 1.0
Identities = 18/28 (64%), Positives = 21/28 (75%), Gaps = 4/28 (14%)
Frame = +2
Query: 58 AAQLGRKVAVVDYVEPSPQGTR----WG 81
AAQ G+KVAV+DYVEPSP+G WG
Sbjct: 863714 AAQFGKKVAVLDYVEPSPKGMNIRQAWG 863797
>gi|397461104|gb|JH734397.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-5488, whole genome shotgun sequence
Length = 134241
Score = 51.6 bits (122), Expect = 9e-05
Identities = 25/49 (51%), Positives = 32/49 (65%)
Frame = +2
Query: 460 VLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVKLR 508
V+GLH G E+ QGFA+ IK GA+ A T+ IHPT +EE+V LR
Sbjct: 104117 VVGLHMQGLGCDEMLQGFAVAIKMGATKADFDNTIAIHPTSAEELVTLR 104263
Score = 45.1 bits (105), Expect = 0.009
Identities = 24/47 (51%), Positives = 29/47 (61%), Gaps = 1/47 (2%)
Frame = +1
Query: 367 LTPIAIMAGRLLVQRLFGGSSDL-MDYDNVPTTVFTPLEYGCVGLSE 412
L +AI AGR L RLF G D +DY N+PT VF+ G VGL+E
Sbjct: 89809 LLTVAIAAGRKLAHRLFEGKKDSKLDYSNIPTVVFSHPPIGTVGLTE 89949
>gi|397460178|gb|JH735323.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-6974, whole genome shotgun sequence
Length = 389885
Score = 42.4 bits (98), Expect = 0.056
Identities = 28/61 (45%), Positives = 37/61 (60%)
Frame = +2
Query: 354 IYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSSDLMDYDNVPTTVFTPLEYGCVGLSEE 413
IYAIGDVV G P L A G L V+ + GG+ + DY+ VP+ ++T E VG SEE
Sbjct: 17348 IYAIGDVVAG-PMLAHKAEDEGILCVEGMAGGAVHI-DYNCVPSVIYTHPEVAWVGKSEE 17521
Query: 414 E 414
+
Sbjct: 17522 Q 17524
>gi|397461636|gb|JH733865.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-4591, whole genome shotgun sequence
Length = 428185
Score = 35.4 bits (80), Expect = 6.8
Identities = 19/82 (23%), Positives = 41/82 (50%)
Frame = -3
Query: 292 LQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREATSV 351
L+ T E T E+ GT+ W++G P +RS A T P ++++ + +V
Sbjct: 387851 LKATKERK*TWSEEGGTYSICTWSLGN-PSSRSFTFRNASCSTKPRSKEMQ*GVTTSPAV 387675
Query: 352 PHIYAIGDVVEGRPELTPIAIM 373
+ ++G +V G+ + ++++
Sbjct: 387674 LNTLSLGTLVGGQDSCSQLSVV 387609
>gi|397464324|gb|JH731177.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-294, whole genome shotgun sequence
Length = 222793
Score = 35.4 bits (80), Expect = 6.8
Identities = 23/68 (33%), Positives = 30/68 (44%), Gaps = 1/68 (1%)
Frame = -1
Query: 63 RKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKKLMHQAALLGGLIQDAPN-YGWEVAQPV 121
R V +P+P WG G T + P L++ A GG P+ GW AQP
Sbjct: 210373 RAVTSASAPQPAPVLAGWGGGATPWSWPKFP*ALVYPVAGAGGDSPPPPSPRGWATAQPH 210194
Query: 122 PHDWRKMA 129
PH W + A
Sbjct: 210193 PHMWEEGA 210170
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis
/genome.fa
Posted date: Oct 18, 2013 5:53 PM
Number of letters in database: 2,174,243,242
Number of sequences in database: 14,644
Lambda K H
0.320 0.137 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,455,290,189
Number of Sequences: 14644
Number of extensions: 24341177
Number of successful extensions: 97341
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 613
Number of HSP's successfully gapped in prelim test: 666
Number of HSP's that attempted gapping in prelim test: 90794
Number of HSP's gapped (non-prelim): 19984
length of query: 524
length of database: 724,747,747
effective HSP length: 134
effective length of query: 390
effective length of database: 722,785,451
effective search space: 281886325890
effective search space used: 281886325890
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 79 (35.0 bits)