TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000129_2.0 # Protein # Thioredoxin reductase 1 (TR1) #
Human (Homo sapiens)
(649 letters)
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g
enome.fa
14,644 sequences; 2,174,243,242 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|397461694|gb|JH733807.1| Alligator mississippiensis unplaced ... 135 3e-44
gi|397458791|gb|JH736710.1| Alligator mississippiensis unplaced ... 155 6e-36
gi|397459659|gb|JH735842.1| Alligator mississippiensis unplaced ... 94 2e-17
gi|397461104|gb|JH734397.1| Alligator mississippiensis unplaced ... 53 5e-05
gi|397463495|gb|JH732006.1| Alligator mississippiensis unplaced ... 47 0.003
gi|397460178|gb|JH735323.1| Alligator mississippiensis unplaced ... 37 2.4
gi|397457179|gb|JH738321.1| Alligator mississippiensis unplaced ... 35 8.9
>gi|397461694|gb|JH733807.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-4487, whole genome shotgun sequence
Length = 316803
Score = 135 bits (341), Expect(2) = 3e-44
Identities = 65/80 (81%), Positives = 73/80 (91%)
Frame = -2
Query: 326 YCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANK 385
+ + SDDL SLPYCPGKTLVVGAS VALECAGFLAGIGLDV+VMVRSILLRGFDQ+MA +
Sbjct: 47744 FFLFSDDLXSLPYCPGKTLVVGASXVALECAGFLAGIGLDVSVMVRSILLRGFDQEMAER 47565
Query: 386 IGEHMEEHGIKFIRQFVPIK 405
IG HME HG+KFIR+FVPI+
Sbjct: 47564 IGAHMETHGVKFIRRFVPIQ 47505
Score = 93.2 bits (230), Expect = 4e-17
Identities = 43/54 (79%), Positives = 52/54 (96%)
Frame = -1
Query: 461 EKTGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGSTVK 514
++ GKIPV+DEEQTNVPY+YAIGDIL+ K+ELTPVAIQAGRLLA+RLYAGS++K
Sbjct: 44919 KRNGKIPVSDEEQTNVPYVYAIGDILDGKLELTPVAIQAGRLLARRLYAGSSIK 44758
Score = 87.0 bits (214), Expect = 3e-15
Identities = 40/59 (67%), Positives = 48/59 (81%)
Frame = -3
Query: 584 RVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTTLSVTKRSGASI 642
RV+GFHVLGPNAGE+TQGFAAA+KCGLTK+ L+ TIGIHP CAEV L + R+ S+
Sbjct: 41785 RVIGFHVLGPNAGEITQGFAAAIKCGLTKELLNETIGIHPTCAEVRGKLELQGRNSLSL 41609
Score = 82.4 bits (202), Expect = 6e-14
Identities = 34/52 (65%), Positives = 44/52 (84%)
Frame = -2
Query: 240 VEETVKHDWDRMIEAVQNHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIK 291
V +VKH+W+ M+EA+QN+IGSLNWGYRV+LREK V Y NAY +FI PH++K
Sbjct: 50783 VSVSVKHNWETMVEAIQNYIGSLNWGYRVSLREKTVTYLNAYAEFIEPHKVK 50628
Score = 76.3 bits (186), Expect = 5e-12
Identities = 33/39 (84%), Positives = 37/39 (94%)
Frame = -3
Query: 204 GLGGTCVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEE 242
GLGGTCVNVGCIPKKLMHQAALLGQAL+DSR +GW+ +E
Sbjct: 52402 GLGGTCVNVGCIPKKLMHQAALLGQALKDSRKFGWQYDE 52286
Score = 69.7 bits (169), Expect = 4e-10
Identities = 29/41 (70%), Positives = 36/41 (87%)
Frame = -1
Query: 511 STVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEV 551
S+ +CDY NVPTTVFTPLEYG CGL EEKA++++GE N+EV
Sbjct: 44508 SSFQCDYVNVPTTVFTPLEYGCCGLPEEKAIKEYGEHNLEV 44386
Score = 68.6 bits (166), Expect(2) = 3e-44
Identities = 30/42 (71%), Positives = 37/42 (88%)
Frame = -3
Query: 291 KATNNKGKEKIYSAERFLIATGERPRYLGIPGDKEYCISSDD 332
+ATN KG+E ++AERF++ATGERPRYLGIPGDKEYCI+ D
Sbjct: 47971 QATNRKGQETYHTAERFVLATGERPRYLGIPGDKEYCITR*D 47846
Score = 63.5 bits (153), Expect = 3e-08
Identities = 25/34 (73%), Positives = 28/34 (82%)
Frame = -1
Query: 551 VYHSYFWPLEWTIPSRDNNKCYAKIICNTKDNER 584
VYH+ FWPLEWT+P+RDNN CYAKIICN N R
Sbjct: 43080 VYHTLFWPLEWTVPNRDNNTCYAKIICNKLANVR 42979
Score = 47.8 bits (112), Expect = 0.002
Identities = 20/34 (58%), Positives = 28/34 (82%)
Frame = -3
Query: 404 IKVEQIEAGTPGRLRVVAQSTNSEEIIEGEYNTV 437
+++E++E G PGRL+VVA+ST +I EGEYNTV
Sbjct: 47245 LQIERLEDGMPGRLKVVARSTEGSDIFEGEYNTV 47144
Score = 43.9 bits (102), Expect = 0.025
Identities = 18/26 (69%), Positives = 24/26 (92%)
Frame = -1
Query: 437 VMLAIGRDACTRKIGLETVGVKINEK 462
V++AIGRDACTR IGL+ +GV+INE+
Sbjct: 46653 VLIAIGRDACTRNIGLDKIGVRINEE 46576
Score = 35.4 bits (80), Expect = 8.9
Identities = 17/33 (51%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = -3
Query: 188 VMVLDFVTPTPLGTRWGLGG-TCVNVGCIPKKL 219
VMVLD+V PTP GT WG C+ + C+ K L
Sbjct: 53620 VMVLDYVVPTPSGTSWGKAQIICLELKCM*KDL 53522
>gi|397458791|gb|JH736710.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-9279, whole genome shotgun sequence
Length = 567381
Score = 155 bits (392), Expect = 6e-36
Identities = 75/81 (92%), Positives = 79/81 (97%)
Frame = -1
Query: 326 YCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANK 385
+ + SDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQ+MANK
Sbjct: 446496 FILFSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQEMANK 446317
Query: 386 IGEHMEEHGIKFIRQFVPIKV 406
IGEHMEEHGIKFI+QFVPIKV
Sbjct: 446316 IGEHMEEHGIKFIKQFVPIKV 446254
Score = 93.2 bits (230), Expect = 4e-17
Identities = 43/53 (81%), Positives = 50/53 (94%)
Frame = -2
Query: 462 KTGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGSTVK 514
++GKIP DEEQTNVPYIYAIGD+LEDK+ELTPVAIQAGRLL +RLYAG+T+K
Sbjct: 440180 RSGKIPANDEEQTNVPYIYAIGDVLEDKLELTPVAIQAGRLLTRRLYAGATLK 440022
Score = 92.4 bits (228), Expect = 6e-17
Identities = 41/46 (89%), Positives = 46/46 (100%)
Frame = -2
Query: 583 ERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEV 628
ER++GFHVLGPNAGEVTQGFAAA+KCGLTK++LDSTIGIHPVCAEV
Sbjct: 433103 ERIIGFHVLGPNAGEVTQGFAAAIKCGLTKEKLDSTIGIHPVCAEV 432966
Score = 90.1 bits (222), Expect(2) = 5e-32
Identities = 39/57 (68%), Positives = 45/57 (78%)
Frame = -2
Query: 240 VEETVKHDWDRMIEAVQNHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIKATNNK 296
V VKH W+ M EAVQN+IGSLNWGYRVALR+ KV YENAYG+F+GPH IK N +
Sbjct: 449156 VFSAVKHSWETMTEAVQNYIGSLNWGYRVALRDNKVTYENAYGEFVGPHTIKVLNKQ 448986
Score = 82.8 bits (203), Expect = 5e-14
Identities = 46/98 (46%), Positives = 50/98 (51%), Gaps = 43/98 (43%)
Frame = -2
Query: 188 VMVLDFVTPTPLGTRWG------------------------------------------- 204
VMVLD+VTPTPLGT WG
Sbjct: 451451 VMVLDYVTPTPLGTSWGNFLLITDIL*RGISYYMALQFYFGVVFIDT*NILCLLSLLPQG 451272
Query: 205 LGGTCVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEE 242
LGGTCVNVGCIPKKLMHQAALLGQAL+DS +GW+ E
Sbjct: 451271 LGGTCVNVGCIPKKLMHQAALLGQALKDSHKFGWQFTE 451158
Score = 73.6 bits (179), Expect(2) = 5e-32
Identities = 32/38 (84%), Positives = 36/38 (94%)
Frame = -1
Query: 292 ATNNKGKEKIYSAERFLIATGERPRYLGIPGDKEYCIS 329
ATN KGKEK+Y+AE+F+IATGERPRYL IPGDKEYCIS
Sbjct: 448878 ATNTKGKEKLYTAEKFIIATGERPRYLNIPGDKEYCIS 448765
Score = 72.4 bits (176), Expect = 7e-11
Identities = 31/38 (81%), Positives = 35/38 (92%)
Frame = -3
Query: 514 KCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEV 551
+CDY NVPTTVFTP+EYGACG SEE+A+EKFGE NIEV
Sbjct: 436741 QCDYVNVPTTVFTPMEYGACGFSEERALEKFGENNIEV 436628
Score = 72.0 bits (175), Expect = 9e-11
Identities = 27/33 (81%), Positives = 31/33 (93%)
Frame = -2
Query: 550 EVYHSYFWPLEWTIPSRDNNKCYAKIICNTKDN 582
+VYHS+FWPLEWT+P RDNNKCYAKIICN +DN
Sbjct: 434801 QVYHSFFWPLEWTVPGRDNNKCYAKIICNIQDN 434703
Score = 52.4 bits (124), Expect = 7e-05
Identities = 22/36 (61%), Positives = 31/36 (86%)
Frame = -1
Query: 133 HGPTLKAYQEGRLQKLLKMNGPEDLPKSYDYDLIII 168
H +++A++EG+LQKLL+MNGP L +SYDYDLI+I
Sbjct: 456519 HFASIQAHEEGKLQKLLEMNGPNHLQESYDYDLIVI 456412
Score = 50.8 bits (120), Expect = 2e-04
Identities = 22/35 (62%), Positives = 29/35 (82%)
Frame = -1
Query: 406 VEQIEAGTPGRLRVVAQSTNSEEIIEGEYNTVMLA 440
VEQIE GTPG+L+V+A+ST + IEGEYNTV ++
Sbjct: 444171 VEQIEEGTPGKLKVIAKSTTGSQTIEGEYNTVSIS 444067
Score = 49.3 bits (116), Expect = 6e-04
Identities = 23/30 (76%), Positives = 27/30 (90%)
Frame = -2
Query: 437 VMLAIGRDACTRKIGLETVGVKINEKTGKI 466
V+LA+GRDACTRKIGL+ VGVKIN+K G I
Sbjct: 442073 VLLAVGRDACTRKIGLDKVGVKINKK*GYI 441984
Score = 42.0 bits (97), Expect = 0.095
Identities = 19/38 (50%), Positives = 28/38 (73%)
Frame = -3
Query: 101 TEDGRALEGTLSELAAETDLPVVFVKQRKIGGHGPTLK 138
TE+G+ L+ L +L ET +PVVFV+Q++IG + TLK
Sbjct: 464035 TENGQKLQEALYQLTLETSVPVVFVQQQQIGNYSKTLK 463922
Score = 37.0 bits (84), Expect = 3.1
Identities = 15/22 (68%), Positives = 20/22 (90%)
Frame = -2
Query: 628 VFTTLSVTKRSGASILQAGCXG 649
+FTTLSVTKRSG +++Q+GC G
Sbjct: 427742 IFTTLSVTKRSGGNVVQSGC*G 427677
>gi|397459659|gb|JH735842.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-7838, whole genome shotgun sequence
Length = 1444372
Score = 94.0 bits (232), Expect = 2e-17
Identities = 38/50 (76%), Positives = 44/50 (88%)
Frame = +1
Query: 199 LGTRWGLGGTCVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEETVKHDW 248
LGT WGLGGTCVNVGCIPKKLMHQAALLG AL+D+R+YGW + + + HDW
Sbjct: 864319 LGTSWGLGGTCVNVGCIPKKLMHQAALLGSALKDARHYGWNISQPIHHDW 864468
Score = 56.6 bits (135), Expect = 4e-06
Identities = 28/46 (60%), Positives = 34/46 (73%)
Frame = +1
Query: 442 GRDACTRKIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILE 487
GR TR + LETVGVK+N +TGKI V E T+VP+IYA+GDI E
Sbjct: 886960 GRVPDTRTLNLETVGVKMNSETGKIIVDASEATSVPHIYAVGDITE 887097
Score = 56.2 bits (134), Expect = 5e-06
Identities = 30/48 (62%), Positives = 34/48 (70%)
Frame = +3
Query: 333 LFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQ 380
+FS Y L+ + V+LECAGFL GIGLD TVMVRSI LRGFDQ
Sbjct: 873420 VFSRNYVTYGPLIGTSPDVSLECAGFLTGIGLDTTVMVRSIPLRGFDQ 873563
Score = 55.5 bits (132), Expect = 8e-06
Identities = 27/45 (60%), Positives = 32/45 (71%)
Frame = +2
Query: 520 VPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIP 564
VPTTVFTPLEYG GLSEEKAV ++GE+N EV + + W P
Sbjct: 893261 VPTTVFTPLEYGCVGLSEEKAVGRYGEDNTEVQKRFL--IGWKEP 893389
Score = 46.2 bits (108), Expect = 0.005
Identities = 21/43 (48%), Positives = 25/43 (58%)
Frame = +3
Query: 607 KCGLTKKQLDSTIGIHPVCAEVFTTLSVTKRSGASILQAGCXG 649
+CG T Q+ T+GIHP AE T L +TKRSG GC G
Sbjct: 898968 RCGATYSQMMKTVGIHPTSAEEVTKLHITKRSGLDATVTGC*G 899096
Score = 42.4 bits (98), Expect = 0.073
Identities = 16/34 (47%), Positives = 25/34 (73%)
Frame = +2
Query: 574 KIICNTKDNERVVGFHVLGPNAGEVTQGFAAALK 607
+++C + +R++G H +GPNAGEV QGFA +K
Sbjct: 896753 QMVCLREKEQRLLGLHFIGPNAGEVIQGFALGIK 896854
Score = 40.4 bits (93), Expect = 0.28
Identities = 17/62 (27%), Positives = 42/62 (67%)
Frame = +1
Query: 382 MANKIGEHMEEHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQSTNSEEIIEGEYNTVMLAI 441
M++ + ++ME HG +F+++ +P KVE++++ G+L+V ++ + + ++TV+ A+
Sbjct: 876136 MSSLVADYMESHGTRFLKKCIPTKVEELDS---GKLQVSWKNADVGKEETDSFDTVIWAV 876306
Query: 442 GR 443
G+
Sbjct: 876307 GK 876312
Score = 37.7 bits (86), Expect = 1.8
Identities = 13/26 (50%), Positives = 20/26 (76%)
Frame = +3
Query: 550 EVYHSYFWPLEWTIPSRDNNKCYAKI 575
+VYH+Y+ PLE+T+ RD +CY K+
Sbjct: 894891 QVYHAYYKPLEFTVAERDATQCYIKV 894968
Score = 37.4 bits (85), Expect = 2.4
Identities = 17/34 (50%), Positives = 26/34 (76%)
Frame = +1
Query: 487 EDKVELTPVAIQAGRLLAQRLYAGSTVKCDYENV 520
+ + ELTP AI AG+LLA+RL++ S+ DY++V
Sbjct: 892882 QGRPELTPTAIAAGKLLARRLFSQSSELMDYDSV 892983
Score = 35.8 bits (81), Expect = 6.8
Identities = 14/23 (60%), Positives = 20/23 (86%)
Frame = +2
Query: 251 MIEAVQNHIGSLNWGYRVALREK 273
M +AVQN++ SLNWG+RV L++K
Sbjct: 864812 MAQAVQNYVKSLNWGHRVQLQDK 864880
>gi|397461104|gb|JH734397.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-5488, whole genome shotgun sequence
Length = 134241
Score = 52.8 bits (125), Expect = 5e-05
Identities = 25/49 (51%), Positives = 30/49 (61%)
Frame = +2
Query: 584 RVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTTL 632
+VVG H+ G E+ QGFA A+K G TK D+TI IHP AE TL
Sbjct: 104114 KVVGLHMQGLGCDEMLQGFAVAIKMGATKADFDNTIAIHPTSAEELVTL 104260
Score = 45.8 bits (107), Expect = 0.007
Identities = 26/52 (50%), Positives = 31/52 (59%), Gaps = 1/52 (1%)
Frame = +1
Query: 490 VELTPVAIQAGRLLAQRLYAGST-VKCDYENVPTTVFTPLEYGACGLSEEKA 540
V L VAI AGR LA RL+ G K DY N+PT VF+ G GL+E K+
Sbjct: 89803 VSLLTVAIAAGRKLAHRLFEGKKDSKLDYSNIPTVVFSHPPIGTVGLTEGKS 89958
>gi|397463495|gb|JH732006.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-1615, whole genome shotgun sequence
Length = 401117
Score = 47.0 bits (110), Expect = 0.003
Identities = 25/63 (39%), Positives = 37/63 (58%), Gaps = 2/63 (3%)
Frame = -3
Query: 54 DSRALL--QAYIDGHSVVIFSRSTCTRCTEVKKLFKSLCVPYFVLELDQTEDGRALEGTL 111
DS + L Q I + VVIFS++TC+ C KKLF+ + V Y +ELD +G L+ L
Sbjct: 44265 DSNSFLNVQETISDNCVVIFSKTTCSYCKMAKKLFQDMNVNYTAVELDMQRNGSQLQEVL 44086
Query: 112 SEL 114
++
Sbjct: 44085 QQM 44077
>gi|397460178|gb|JH735323.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-6974, whole genome shotgun sequence
Length = 389885
Score = 37.4 bits (85), Expect = 2.4
Identities = 17/43 (39%), Positives = 29/43 (67%)
Frame = +2
Query: 435 NTVMLAIGRDACTRKIGLETVGVKINEKTGKIPVTDEEQTNVP 477
+ +++ IGR T+ +GLE +GV+++ K G+IPV QT +P
Sbjct: 16694 DVLLVCIGRRPFTKNLGLEDLGVELDNK-GRIPVNTRFQTKIP 16819
>gi|397457179|gb|JH738321.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-11991, whole genome shotgun sequence
Length = 437663
Score = 35.4 bits (80), Expect = 8.9
Identities = 23/66 (34%), Positives = 30/66 (45%), Gaps = 1/66 (1%)
Frame = -3
Query: 508 YAGSTVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIPSRD 567
Y G+ KC Y+N P TVF Y + + K+ G E I + PL IP
Sbjct: 364854 YKGNKTKCRYQNSPATVFDDNLYAL--MLQSKSFS*LGMEAIRLLKCSL*PLGEKIPCTS 364681
Query: 568 NNK-CY 572
N+K CY
Sbjct: 364680 NSKVCY 364663
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis
/genome.fa
Posted date: Oct 18, 2013 5:53 PM
Number of letters in database: 2,174,243,242
Number of sequences in database: 14,644
Lambda K H
0.318 0.137 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,706,465,670
Number of Sequences: 14644
Number of extensions: 26456195
Number of successful extensions: 111237
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 493
Number of HSP's successfully gapped in prelim test: 360
Number of HSP's that attempted gapping in prelim test: 106022
Number of HSP's gapped (non-prelim): 16314
length of query: 649
length of database: 724,747,747
effective HSP length: 136
effective length of query: 513
effective length of database: 722,756,163
effective search space: 370773911619
effective search space used: 370773911619
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 80 (35.4 bits)