TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000124_2.0 # Protein # Selenoprotein W1 (SelW1) # Human (Homo sapiens) (87 letters) Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g enome.fa 14,644 sequences; 2,174,243,242 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|397461887|gb|JH733614.1| Alligator mississippiensis unplaced ... 39 0.030 >gi|397461887|gb|JH733614.1| Alligator mississippiensis unplaced genomic scaffold scaffold-4174, whole genome shotgun sequence Length = 1203201 Score = 39.3 bits (90), Expect = 0.030 Identities = 17/23 (73%), Positives = 20/23 (86%) Frame = +1 Query: 39 EGTPQATGFFEVMVAGKLIHSKK 61 EGT +ATG+FEV V GKL+HSKK Sbjct: 545692 EGTREATGWFEVSVGGKLVHSKK 545760 Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis /genome.fa Posted date: Oct 18, 2013 5:53 PM Number of letters in database: 2,174,243,242 Number of sequences in database: 14,644 Lambda K H 0.318 0.140 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 135,592,621 Number of Sequences: 14644 Number of extensions: 1163708 Number of successful extensions: 3177 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 153 Number of HSP's successfully gapped in prelim test: 23 Number of HSP's that attempted gapping in prelim test: 3000 Number of HSP's gapped (non-prelim): 279 length of query: 87 length of database: 724,747,747 effective HSP length: 62 effective length of query: 25 effective length of database: 723,839,819 effective search space: 18095995475 effective search space used: 18095995475 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 68 (30.8 bits)