TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000123_2.0 # Protein # Selenoprotein V (SelV) # Human (Homo sapiens) (346 letters) Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g enome.fa 14,644 sequences; 2,174,243,242 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|397456032|gb|JH739468.1| Alligator mississippiensis unplaced ... 38 0.76 gi|397463659|gb|JH731842.1| Alligator mississippiensis unplaced ... 35 4.9 >gi|397456032|gb|JH739468.1| Alligator mississippiensis unplaced genomic scaffold scaffold-13950, whole genome shotgun sequence Length = 220046 Score = 37.7 bits (86), Expect = 0.76 Identities = 17/27 (62%), Positives = 17/27 (62%) Frame = -2 Query: 179 PSVSSEAGPAPGPLPTRTPLAANSPGP 205 PS S A PAP P P RTPL NSP P Sbjct: 96430 PSPSMAASPAPAPGPLRTPLTLNSPAP 96350 >gi|397463659|gb|JH731842.1| Alligator mississippiensis unplaced genomic scaffold scaffold-1353, whole genome shotgun sequence Length = 235689 Score = 35.0 bits (79), Expect = 4.9 Identities = 16/37 (43%), Positives = 22/37 (59%) Frame = -2 Query: 183 SEAGPAPGPLPTRTPLAANSPGPTLDFTFRADPSAIG 219 +EAG PGP P+R PL + P P+ DP+A+G Sbjct: 144581 AEAGSWPGPDPSRQPLPTSWPAPSRGSCRNPDPAALG 144471 Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis /genome.fa Posted date: Oct 18, 2013 5:53 PM Number of letters in database: 2,174,243,242 Number of sequences in database: 14,644 Lambda K H 0.316 0.133 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 492,221,114 Number of Sequences: 14644 Number of extensions: 6459644 Number of successful extensions: 91862 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 1868 Number of HSP's successfully gapped in prelim test: 762 Number of HSP's that attempted gapping in prelim test: 74848 Number of HSP's gapped (non-prelim): 35518 length of query: 346 length of database: 724,747,747 effective HSP length: 130 effective length of query: 216 effective length of database: 722,844,027 effective search space: 156134309832 effective search space used: 156134309832 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 76 (33.9 bits)