TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000106_2.0 # Protein # Selenoprotein T (SelT) # Human (Homo sapiens) (195 letters) Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g enome.fa 14,644 sequences; 2,174,243,242 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|397463993|gb|JH731508.1| Alligator mississippiensis unplaced ... 95 1e-18 >gi|397463993|gb|JH731508.1| Alligator mississippiensis unplaced genomic scaffold scaffold-851, whole genome shotgun sequence Length = 1392873 Score = 95.1 bits (235), Expect = 1e-18 Identities = 44/53 (83%), Positives = 48/53 (90%) Frame = -2 Query: 83 RHIASFLSVFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENKVYACMMVFFL 135 RHIASFLS+FKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENKV+ + F+ Sbjct: 547238 RHIASFLSIFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENKVHDLLFFSFV 547080 Score = 86.3 bits (212), Expect = 6e-16 Identities = 39/41 (95%), Positives = 41/41 (100%) Frame = -1 Query: 155 DVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMDSIPHHRS 195 DVPVWSKLESGHLPSMQQLVQILDNEMKLNVHM+S+PHHRS Sbjct: 544389 DVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMESMPHHRS 544267 Score = 78.6 bits (192), Expect = 1e-13 Identities = 36/39 (92%), Positives = 38/39 (97%) Frame = -2 Query: 47 VSXGYRRVFEEYMRVISQRYPDIRIEGENYLPQPIYRHI 85 VS GYRRVFEEYMRVISQRYPDIRIEGENYLPQPIYR++ Sbjct: 548285 VS*GYRRVFEEYMRVISQRYPDIRIEGENYLPQPIYRYV 548169 Score = 63.2 bits (152), Expect = 6e-09 Identities = 29/29 (100%), Positives = 29/29 (100%) Frame = -2 Query: 126 VYACMMVFFLSNMIENQCMSTGAFEITLN 154 VYACMMVFFLSNMIENQCMSTGAFEITLN Sbjct: 545123 VYACMMVFFLSNMIENQCMSTGAFEITLN 545037 Score = 47.8 bits (112), Expect = 2e-04 Identities = 24/40 (60%), Positives = 32/40 (80%), Gaps = 1/40 (2%) Frame = -3 Query: 9 VAASAMVRSEASANLGGVPSKRLKMQYATGPLLKFQI-CV 47 VAA+A + + A+ GG+P+K+L+M YATGPLLKFQI CV Sbjct: 552298 VAAAAGLSAAAAGEPGGLPAKKLRMAYATGPLLKFQIWCV 552179 Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis /genome.fa Posted date: Oct 18, 2013 5:53 PM Number of letters in database: 2,174,243,242 Number of sequences in database: 14,644 Lambda K H 0.325 0.137 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 510,385,916 Number of Sequences: 14644 Number of extensions: 7609545 Number of successful extensions: 48623 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 705 Number of HSP's successfully gapped in prelim test: 462 Number of HSP's that attempted gapping in prelim test: 45285 Number of HSP's gapped (non-prelim): 9533 length of query: 195 length of database: 724,747,747 effective HSP length: 122 effective length of query: 73 effective length of database: 722,961,179 effective search space: 52776166067 effective search space used: 52776166067 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 72 (32.3 bits)