Command line: [exonerate -m p2g --showtargetgff -q ../Sel.tortu/SELP.fa -t gi|397460716|gb|JH734785.1|subseq.fa -E] Hostname: [ws101145] ** (process:3330): WARNING **: Exhaustively generating suboptimal alignments will be VERY SLOW C4 Alignment: ------------ Query: SPP00002140_2.0 # Protein # Selenoprotein P (SelP) # Softshell turtle (Pelodiscus sinensis) Target: gi|397460716|gb|JH734785.1|:subseq(44689,103000) Alligator mississippiensis unplaced genomic scaffold scaffold-6128, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 691 Query range: 14 -> 201 Target range: 52289 -> 17448 15 : ValAlaGluAsnLysThrArgIleCysGlnProAlaProGlnTrpAlaIleAsnGlyThr : 34 :!!|||||| !! !|||||||||||||||||||||! !||| !|||||||||||| LeuAlaGluValThrAspArgIleCysGlnProAlaProLeuTrpLysIleAsnGlyThr 52289 : CTGGCGGAAGTCACCGACCGCATCTGCCAGCCAGCTCCGCTGTGGAAGATCAATGGGACG : 52232 35 : AlaProMetLysArgGluLeuGlyArgValThrValValAlaLeuLeuLysAlaSerUnk : 54 !.!|||||| ! ..!! !||||||!:!|||||||||||||||||||||||||||||| ValProMetAspGluAlaLeuGlyGlnValThrValValAlaLeuLeuLysAlaSer*** 52231 : GTCCCGATGGACGAGGCGCTGGGGCAGGTGACGGTGGTGGCGCTTTTGAAGGCCAGCTGA : 52172 55 : GlnPheCysLeuLysGlnAlaHis{Se} >>>> Target Intron 1 >>>> {r} : 63 |||||||||||||||||||||:!!{||} 119 bp {|} GlnPheCysLeuLysGlnAlaAsn{Se}++ ++{r} 52171 : CAGTTCTGTCTCAAGCAAGCTAAC{AG}gt.........................ag{C} : 52024 64 : LeuGlyGlyLeuArgAlaLysLeuSerArgAspGlyLeuThrAspIleSerTyrMetIle : 83 |||||||||||||||! !||||||||||||.!.|||:!!.!!:!!:!!|||||||||||| LeuGlyGlyLeuArgGluLysLeuSerArgGlnGlyMetAlaAsnValSerTyrMetIle 52023 : CTTGGCGGCTTGCGCGAGAAGCTGTCCCGGCAAGGGATGGCCAACGTCAGCTACATGATC : 51966 84 : ValAsnGluLysAlaAlaLeuSerArgAlaMetTyrTrpGlnLeuLysArgGlnAlaPro : 103 ||||||||||||||| !!|||||||||||||||||||||:!!|||||||||||||||||| ValAsnGluLysAlaProLeuSerArgAlaMetTyrTrpGluLeuLysArgGlnAlaPro 51965 : GTGAATGAGAAGGCGCCGCTGTCCCGGGCCATGTACTGGGAGCTGAAGCGCCAGGCCCCC : 51906 104 : LysGlyValProValTyrGlnGlnGluLeuLeuGluProAspValTrpGlnIleLeuAsp : 123 :!!|||||||||||||||||||||! !:!!|||!!:|||||||||||||||||||||||| GluGlyValProValTyrGlnGlnGlyIleLeuAspProAspValTrpGlnIleLeuAsp 51905 : GAGGGGGTGCCCGTGTACCAGCAGGGGATCCTGGACCCCGACGTCTGGCAGATCCTGGAT : 51846 124 : GlyAspLysAspAspPheLeuIleTyrAsp{Ar} >>>> Target Intron 2 >>> : 134 ||||||||||||||||||||||||||||||{!:} 1676 bp GlyAspLysAspAspPheLeuIleTyrAsp{Ly}++ 51845 : GGGGATAAGGACGACTTCCTCATCTACGAC{AA}gt........................ : 51809 135 : > {g}CysGlyArgLeuAlaPheHisIleProLeuProTyrSerPheLeuHisLeuArg : 152 {!}||||||||||||!.!||||||||| !!|||||||||||||||||||||!!!! ! ++{s}CysGlyArgLeuValPheHisIleSerLeuProTyrSerPheLeuHisPhePro 51808 : .ag{G}TGCGGCCGCTTGGTCTTCCACATCTCGCTGCCCTACAGCTTCCTGCACTTCCCC : 50083 153 : TyrValGluSerAlaValArgValThrHisAlaLysAspPheCysGlyAsnCysSerPhe : 172 ||||||||||||||||||!.! !!|||:!!.!!||||||!:!||||||||||||! ! ! TyrValGluSerAlaValHisPheThrTyrThrLysAspTyrCysGlyAsnCysIleHis 50082 : TACGTGGAGTCCGCCGTGCACTTCACGTACACCAAGGATTACTGCGGGAACTGCATCCAC : 50023 173 : TyrSerAsnSerThrGlnGluVal >>>> Target Intron 3 >>>> ArgAsnH : 183 ||||||||||||||||||:!!! ! 11488 bp ! ! | TyrSerAsnSerThrGlnGlnGlu++ ++ProLeuH 50022 : TACTCCAACAGCACCCAGCAAGAGgt.........................agCCCCTGC : 38502 184 : isSerArgAlaArgAlaLeuThrValAlaSer{G} >>>> Target Intron 4 >> : 194 || !!:!|||! !!.!! ! !:!!||||||{|} 20997 bp isLeuGlnAlaProGlyGlnGluIleAlaSer{G}-+ 38501 : ACCTGCAGGCACCAGGCCAGGAAATTGCTAGT{G}tt....................... : 38466 195 : >> {ly}CysAlaProArgIleThrArg : 201 {||}||| ! ! !:!:.!!||| ++{ly}CysGlnGluAlaValAlaArg 38465 : ..ag{GC}TGCCAGGAGGCAGTGGCAAGG : 17449 vulgar: SPP00002140_2.0 14 201 . gi|397460716|gb|JH734785.1|:subseq(44689,103000) 52289 17448 - 691 M 48 144 S 0 2 5 0 2 I 0 115 3 0 2 S 1 1 M 70 210 S 0 2 5 0 2 I 0 1672 3 0 2 S 1 1 M 46 138 5 0 2 I 0 11484 3 0 2 M 13 39 S 0 1 5 0 2 I 0 20993 3 0 2 S 1 2 M 7 21 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-20 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local gene 17449 52289 691 - . gene_id 1 ; sequence SPP00002140_2.0 ; gene_orientation + gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local cds 52144 52289 . - . gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local exon 52144 52289 . - . insertions 0 ; deletions 0 gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local splice5 52142 52143 . - . intron_id 1 ; splice_site "GT" gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local intron 52025 52143 . - . intron_id 1 gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local splice3 52025 52026 . - . intron_id 0 ; splice_site "AG" gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local cds 51812 52024 . - . gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local exon 51812 52024 . - . insertions 0 ; deletions 0 gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local splice5 51810 51811 . - . intron_id 2 ; splice_site "GT" gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local intron 50136 51811 . - . intron_id 2 gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local splice3 50136 50137 . - . intron_id 1 ; splice_site "AG" gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local cds 49997 50135 . - . gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local exon 49997 50135 . - . insertions 0 ; deletions 0 gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local splice5 49995 49996 . - . intron_id 3 ; splice_site "GT" gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local intron 38509 49996 . - . intron_id 3 gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local splice3 38509 38510 . - . intron_id 2 ; splice_site "AG" gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local cds 38469 38508 . - . gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local exon 38469 38508 . - . insertions 0 ; deletions 0 gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local splice5 38467 38468 . - . intron_id 4 ; splice_site "TT" gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local intron 17472 38468 . - . intron_id 4 gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local splice3 17472 17473 . - . intron_id 3 ; splice_site "AG" gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local cds 17449 17471 . - . gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local exon 17449 17471 . - . insertions 0 ; deletions 0 gi|397460716|gb|JH734785.1|:subseq(44689,103000) exonerate:protein2genome:local similarity 17449 52289 691 - . alignment_id 1 ; Query SPP00002140_2.0 ; Align 52290 15 144 ; Align 52024 64 210 ; Align 50135 135 138 ; Align 38509 181 39 ; Align 17470 195 21 # --- END OF GFF DUMP --- # -- completed exonerate analysis