TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00002140_2.0 # Protein # Selenoprotein P (SelP) # Softshell turtle (Pelodiscus sinensis) (201 letters) Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g enome.fa 14,644 sequences; 2,174,243,242 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|397460716|gb|JH734785.1| Alligator mississippiensis unplaced ... 129 3e-45 gi|397460395|gb|JH735106.1| Alligator mississippiensis unplaced ... 75 1e-12 gi|397456242|gb|JH739258.1| Alligator mississippiensis unplaced ... 33 8.8 gi|397458990|gb|JH736511.1| Alligator mississippiensis unplaced ... 33 8.8 >gi|397460716|gb|JH734785.1| Alligator mississippiensis unplaced genomic scaffold scaffold-6128, whole genome shotgun sequence Length = 359352 Score = 129 bits (323), Expect(2) = 3e-45 Identities = 59/72 (81%), Positives = 67/72 (93%) Frame = -3 Query: 63 SLGGLRAKLSRDGLTDISYMIVNEKAALSRAMYWQLKRQAPKGVPVYQQELLEPDVWQIL 122 SLGGLR KLSR G+ ++SYMIVNEKA LSRAMYW+LKRQAP+GVPVYQQ +L+PDVWQIL Sbjct: 96715 SLGGLREKLSRQGMANVSYMIVNEKAPLSRAMYWELKRQAPEGVPVYQQGILDPDVWQIL 96536 Query: 123 DGDKDDFLIYDR 134 DGDKDDFLIYD+ Sbjct: 96535 DGDKDDFLIYDK 96500 Score = 78.6 bits (192), Expect = 1e-13 Identities = 34/46 (73%), Positives = 37/46 (80%) Frame = -2 Query: 134 RCGRLAFHIPLPYSFLHLRYVESAVRVTHAKDFCGNCSFYSNSTQE 179 RCGRL FHI LPYSFLH YVESAV T+ KD+CGNC YSNSTQ+ Sbjct: 94826 RCGRLVFHISLPYSFLHFPYVESAVHFTYTKDYCGNCIHYSNSTQQ 94689 Score = 76.3 bits (186), Expect(2) = 3e-45 Identities = 41/62 (66%), Positives = 45/62 (72%) Frame = -1 Query: 1 MATLLGLVVATPEDVAENKTRICQPAPQWAINGTAPMKRELGRVTVVALLKASXQFCLKQ 60 +A LLGLV +AE RICQPAP W INGT PM LG+VTVVALLKAS QFCLKQ Sbjct: 97011 VAALLGLVATV---LAEVTDRICQPAPLWKINGTVPMDEALGQVTVVALLKAS*QFCLKQ 96841 Query: 61 AH 62 A+ Sbjct: 96840 AN 96835 >gi|397460395|gb|JH735106.1| Alligator mississippiensis unplaced genomic scaffold scaffold-6630, whole genome shotgun sequence Length = 852651 Score = 75.5 bits (184), Expect = 1e-12 Identities = 36/72 (50%), Positives = 49/72 (68%) Frame = -2 Query: 63 SLGGLRAKLSRDGLTDISYMIVNEKAALSRAMYWQLKRQAPKGVPVYQQELLEPDVWQIL 122 SL LR KL DGL +ISYM+VN + S+ L+++ + + VYQQ+ +PDVW L Sbjct: 228485 SLEDLRVKLENDGLVNISYMVVNHQEITSQEKIHLLRKEVSEHIVVYQQDENQPDVWTTL 228306 Query: 123 DGDKDDFLIYDR 134 +G+KDDFLIYDR Sbjct: 228305 NGNKDDFLIYDR 228270 Score = 53.1 bits (126), Expect = 6e-06 Identities = 18/39 (46%), Positives = 29/39 (74%) Frame = -3 Query: 134 RCGRLAFHIPLPYSFLHLRYVESAVRVTHAKDFCGNCSF 172 RCGRL +H+ LPY++L YVE ++++ + + CGNCS+ Sbjct: 227680 RCGRLVYHLELPYTYLSFPYVEESIKIAYCEQNCGNCSY 227564 Score = 42.4 bits (98), Expect = 0.011 Identities = 21/39 (53%), Positives = 24/39 (61%) Frame = -3 Query: 23 CQPAPQWAINGTAPMKRELGRVTVVALLKASXQFCLKQA 61 C+ P+W I G PM G VTVVALL+AS CL QA Sbjct: 230497 CKEPPEWYIAGQDPMLNSRGSVTVVALLQAS*HLCLLQA 230381 >gi|397456242|gb|JH739258.1| Alligator mississippiensis unplaced genomic scaffold scaffold-13578, whole genome shotgun sequence Length = 606661 Score = 32.7 bits (73), Expect = 8.8 Identities = 17/40 (42%), Positives = 23/40 (57%) Frame = +2 Query: 143 PLPYSFLHLRYVESAVRVTHAKDFCGNCSFYSNSTQEVRN 182 PLP S LH S+V THA F G+ F ++T+ +RN Sbjct: 44057 PLPLSLLHYCIFYSSVAPTHASKF*GSGIFKLDNTKSMRN 44176 >gi|397458990|gb|JH736511.1| Alligator mississippiensis unplaced genomic scaffold scaffold-8948, whole genome shotgun sequence Length = 255580 Score = 32.7 bits (73), Expect = 8.8 Identities = 22/65 (33%), Positives = 30/65 (46%) Frame = +2 Query: 134 RCGRLAFHIPLPYSFLHLRYVESAVRVTHAKDFCGNCSFYSNSTQEVRNHSRARALTVAS 193 RCG HIP ++ LH +++S + + K +CG CS VR H R T A Sbjct: 37454 RCG----HIPFSWAHLHEMFMQS*LINSTVKCYCGICS--------VRPHCGERG*TTAV 37597 Query: 194 GCAPR 198 C R Sbjct: 37598 VCTAR 37612 Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis /genome.fa Posted date: Oct 18, 2013 5:53 PM Number of letters in database: 2,174,243,242 Number of sequences in database: 14,644 Lambda K H 0.323 0.135 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 612,512,525 Number of Sequences: 14644 Number of extensions: 10196892 Number of successful extensions: 38279 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 745 Number of HSP's successfully gapped in prelim test: 405 Number of HSP's that attempted gapping in prelim test: 35524 Number of HSP's gapped (non-prelim): 7637 length of query: 201 length of database: 724,747,747 effective HSP length: 123 effective length of query: 78 effective length of database: 722,946,535 effective search space: 56389829730 effective search space used: 56389829730 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 73 (32.7 bits)