TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000082_2.0 # Protein # Selenoprotein P (SelP) # Human (Homo sapiens) (381 letters) Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g enome.fa 14,644 sequences; 2,174,243,242 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|397460395|gb|JH735106.1| Alligator mississippiensis unplaced ... 102 2e-20 gi|397460716|gb|JH734785.1| Alligator mississippiensis unplaced ... 75 8e-18 gi|397459915|gb|JH735586.1| Alligator mississippiensis unplaced ... 34 9.7 >gi|397460395|gb|JH735106.1| Alligator mississippiensis unplaced genomic scaffold scaffold-6630, whole genome shotgun sequence Length = 852651 Score = 102 bits (255), Expect = 2e-20 Identities = 50/71 (70%), Positives = 59/71 (83%) Frame = -2 Query: 69 LEDLRVKLKKEGYSNISYIVVNHQGISSRLKYTHLKNKVSEHIPVYQQEENQTDVWTLLN 128 LEDLRVKL+ +G NISY+VVNHQ I+S+ K L+ +VSEHI VYQQ+ENQ DVWT LN Sbjct: 228482 LEDLRVKLENDGLVNISYMVVNHQEITSQEKIHLLRKEVSEHIVVYQQDENQPDVWTTLN 228303 Query: 129 GSKDDFLIYDR 139 G+KDDFLIYDR Sbjct: 228302 GNKDDFLIYDR 228270 Score = 84.0 bits (206), Expect = 1e-14 Identities = 42/70 (60%), Positives = 47/70 (67%) Frame = -3 Query: 1 MWRSXXXXXXXXXXPSGGTESQDQSSLCKQPPAWSIRDQDPMLNSNGSVTVVALLQASXY 60 MW P GGTESQ S+ CK+PP W I QDPMLNS GSVTVVALLQAS + Sbjct: 230578 MWAGLGLALVLCLLPEGGTESQGSSAHCKEPPEWYIAGQDPMLNSRGSVTVVALLQAS*H 230399 Query: 61 LCILQASKLE 70 LC+LQAS+ E Sbjct: 230398 LCLLQASR*E 230369 Score = 74.3 bits (181), Expect = 8e-12 Identities = 30/38 (78%), Positives = 36/38 (94%) Frame = -3 Query: 139 RCGRLVYHLGLPFSFLTFPYVEEAIKIAYCEKKCGNCS 176 RCGRLVYHL LP+++L+FPYVEE+IKIAYCE+ CGNCS Sbjct: 227680 RCGRLVYHLELPYTYLSFPYVEESIKIAYCEQNCGNCS 227567 Score = 58.2 bits (139), Expect = 6e-07 Identities = 36/73 (49%), Positives = 39/73 (53%) Frame = -1 Query: 281 CINQLLCKLPTDSELAPRSXCCHCRHLIFEKTGSAITXQCKENLPSLCSXQGLRAEENIT 340 C NQL SE S C H RHL+FE+ G A T C+ LP C QG ENIT Sbjct: 224682 CKNQLT*NFLKASESTSSS*C*H*RHLLFEEVGKAAT*HCRGTLPDTCR*QGQLLLENIT 224503 Query: 341 ESCQXRLPPAAXQ 353 ES Q RL PAA Q Sbjct: 224502 ES*Q*RLLPAA*Q 224464 >gi|397460716|gb|JH734785.1| Alligator mississippiensis unplaced genomic scaffold scaffold-6128, whole genome shotgun sequence Length = 359352 Score = 74.7 bits (182), Expect(2) = 8e-18 Identities = 35/71 (49%), Positives = 47/71 (66%) Frame = -3 Query: 69 LEDLRVKLKKEGYSNISYIVVNHQGISSRLKYTHLKNKVSEHIPVYQQEENQTDVWTLLN 128 L LR KL ++G +N+SY++VN + SR Y LK + E +PVYQQ DVW +L+ Sbjct: 96712 LGGLREKLSRQGMANVSYMIVNEKAPLSRAMYWELKRQAPEGVPVYQQGILDPDVWQILD 96533 Query: 129 GSKDDFLIYDR 139 G KDDFLIYD+ Sbjct: 96532 GDKDDFLIYDK 96500 Score = 58.9 bits (141), Expect = 4e-07 Identities = 23/37 (62%), Positives = 28/37 (75%) Frame = -2 Query: 139 RCGRLVYHLGLPFSFLTFPYVEEAIKIAYCEKKCGNC 175 RCGRLV+H+ LP+SFL FPYVE A+ Y + CGNC Sbjct: 94826 RCGRLVFHISLPYSFLHFPYVESAVHFTYTKDYCGNC 94716 Score = 40.0 bits (92), Expect(2) = 8e-18 Identities = 19/42 (45%), Positives = 26/42 (61%) Frame = -1 Query: 27 LCKQPPAWSIRDQDPMLNSNGSVTVVALLQASXYLCILQASK 68 +C+ P W I PM + G VTVVALL+AS C+ QA++ Sbjct: 96957 ICQPAPLWKINGTVPMDEALGQVTVVALLKAS*QFCLKQANR 96832 >gi|397459915|gb|JH735586.1| Alligator mississippiensis unplaced genomic scaffold scaffold-7408, whole genome shotgun sequence Length = 721727 Score = 34.3 bits (77), Expect = 9.7 Identities = 21/51 (41%), Positives = 25/51 (49%), Gaps = 1/51 (1%) Frame = +2 Query: 98 LKYTHLKNKVSEHIPVYQQEENQTDVWTLLNG-SKDDFLIYDRCGRLVYHL 147 LK+ +SEHI VY Q V LNG +K DF Y + G L HL Sbjct: 462893 LKFAKSIKFISEHIDVYAQAHTHFGVILCLNGLTKIDFSFYKKTGTLSSHL 463045 Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis /genome.fa Posted date: Oct 18, 2013 5:53 PM Number of letters in database: 2,174,243,242 Number of sequences in database: 14,644 Lambda K H 0.315 0.130 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 1,013,393,195 Number of Sequences: 14644 Number of extensions: 16022485 Number of successful extensions: 72656 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 590 Number of HSP's successfully gapped in prelim test: 439 Number of HSP's that attempted gapping in prelim test: 68449 Number of HSP's gapped (non-prelim): 12662 length of query: 381 length of database: 724,747,747 effective HSP length: 131 effective length of query: 250 effective length of database: 722,829,383 effective search space: 180707345750 effective search space used: 180707345750 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 77 (34.3 bits)