Command line: [exonerate -m p2g --showtargetgff -q ../Sel.human/SELO.fa -t gi|397461378|gb|JH734123.1|subseq.fa] Hostname: [ws101062] C4 Alignment: ------------ Query: SPP00000081_2.0 # Protein # Selenoprotein O (SelO) # Human (Homo sapiens) Target: gi|397461378|gb|JH734123.1|:subseq(1630000,100000) Alligator mississippiensis unplaced genomic scaffold scaffold-5035, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 2090 Query range: 103 -> 627 Target range: 70748 -> 50896 104 : AlaLeuLeuGlyLeuGlyAlaProProAlaArgGluAlaGluAlaGluAlaAlaLeuPhe : 123 ||||||||||||||| !! !!||| !!!.! !||||||||| !||||||||||||! ! AlaLeuLeuGlyLeuArgProProAlaGlySerGluAlaGluAsnGluAlaAlaLeuCys 70748 : GCGCTGCTGGGGCTGCGGCCGCCGGCGGGGTCCGAGGCCGAGAACGAGGCCGCGCTGTGC : 70691 124 : PheSerGlyAsnAlaLeuLeuProGlyAlaGluProAlaAlaHisCysTyrCysGlyHis : 143 |||||||||||| !|||! !||||||||||||||||||||||||||||||||||||||| PheSerGlyAsnArgLeuProProGlyAlaGluProAlaAlaHisCysTyrCysGlyHis 70690 : TTCAGCGGCAACCGCCTGCCGCCCGGCGCCGAGCCCGCCGCGCACTGCTACTGCGGCCAC : 70631 144 : GlnPheGlyGlnPheAlaGlyGlnLeuGlyAspGlyAlaAlaMetTyrLeuGlyGluVal : 163 |||||||||...|||||||||||||||||||||||||||||||||||||||||||||||| GlnPheGlySerPheAlaGlyGlnLeuGlyAspGlyAlaAlaMetTyrLeuGlyGluVal 70630 : CAGTTCGGCAGCTTCGCGGGCCAGCTGGGCGACGGCGCCGCCATGTACCTGGGCGAGGTG : 70571 164 : CysThrAlaThrGlyGluArgTrpGluLeuGlnLeuLysGlyAlaGlyProThrProPhe : 183 ! !!.!!|||..!|||||||||:!!||||||||||||||||||! !||||||||| LeuGlyProAlaGlyArgArgTrpGluValGlnLeuLysGlyAlaGlyLeuThrProPhe 70570 : CTGGGCCCGGCCGGGCGGCGCTGGGAGGTGCAGCTCAAGGGCGCCGGCCTCACGCCCTTC : 70511 184 : Ser{Ar} >>>> Target Intron 1 >>>> {g}GlnAlaAspGlyArgLysVal : 192 |||{||} 4704 bp {|}||||||||||||||||||||| Ser{Ar}++ ++{g}GlnAlaAspGlyArgLysVal 70510 : TCC{CG}gt.........................ag{G}CAAGCTGATGGTCGTAAGGTC : 65780 193 : LeuArgSerSerIleArgGluPheLeuCysSerGluAlaMetPheHisLeuGlyValPro : 212 ||||||||||||||||||||||||||||||||||||||||||||||||||||||:!!||| LeuArgSerSerIleArgGluPheLeuCysSerGluAlaMetPheHisLeuGlyIlePro 65779 : CTGCGGTCAAGTATAAGAGAATTCCTGTGCAGTGAGGCTATGTTTCACCTTGGAATACCC : 65720 213 : ThrThrArgAlaGlyAlaCysValThrSerGluSerThrValValArgAspValPheTyr : 232 |||||||||||||||.!!||||||||||||!!:|||! !|||:!!||||||||||||||| ThrThrArgAlaGlyThrCysValThrSerAspSerLysValIleArgAspValPheTyr 65719 : ACAACAAGGGCTGGAACATGTGTAACATCTGACTCGAAAGTTATTCGGGATGTATTCTAT : 65660 233 : AspGlyAsnProLysTyrGluGlnCysThrValValLeuArgValAlaSerThrPheIle : 252 |||||||||||||||:!!|||::!||||||||||||||||||:!!||||||||||||||| AspGlyAsnProLysHisGluArgCysThrValValLeuArgIleAlaSerThrPheIle 65659 : GATGGAAATCCAAAACATGAAAGATGCACAGTTGTCCTGAGAATAGCTTCCACTTTTATA : 65600 253 : {Ar} >>>> Target Intron 2 >>>> {g}PheGlySerPheGluIlePheLys : 261 {||} 944 bp {|}|||||||||||||||||||||||| {Ar}++ ++{g}PheGlySerPheGluIlePheLys 65599 : {AG}gt.........................ag{G}TTTGGATCATTTGAGATTTTTAAA : 64629 262 : SerAlaAspGluHisThrGlyArgAlaGlyProSerValGlyArgAsnAspIleArgVal : 281 !! !|||||||||||||||||| !||||||||||||..!|||||||||||||||..! ProMetAspGluHisThrGlyArgLysGlyProSerValAsnArgAsnAspIleArgThr 64628 : CCTATGGATGAACATACAGGACGGAAGGGTCCCAGTGTCAACCGAAATGATATTCGAACA : 64569 282 : GlnLeuLeuAspTyrValIleSerSerPheTyrProGluIleGlnAlaAlaHisAlaSer : 301 |||:!!|||:!!||||||||||||!!!|||||||||||||||||| !|||:!! !!..! GlnMetLeuAsnTyrValIleSerThrPheTyrProGluIleGlnGlnAlaTyrProAsp 64568 : CAGATGCTTAACTACGTGATCAGTACTTTCTATCCAGAAATCCAGCAGGCTTATCCTGAC : 64509 302 : AspSerValGlnArgAsnAlaAlaPhePheArgGlu >>>> Target Intron 3 > : 314 :!!!!!:!!||||||||||||||||||||||||||| 6245 bp AsnThrIleGlnArgAsnAlaAlaPhePheArgGlu++ 64508 : AACACTATTCAGAGGAATGCTGCTTTCTTCAGAGAGgt...................... : 64468 315 : >>> ValThrArgArgThrAlaArgMetValAlaGluTrpGlnCysValGlyPheCysH : 332 :!!|||!:!||||||||||||:!!||||||||||||||||||||||||||||||| ++IleThrLysArgThrAlaArgLeuValAlaGluTrpGlnCysValGlyPheCysH 64467 : ...agATAACAAAGCGGACAGCAAGGTTGGTTGCTGAATGGCAGTGTGTTGGATTTTGCC : 58171 333 : isGlyValLeuAsnThrAspAsnMetSerIleLeuGlyLeuThrIleAspTyrGlyProP : 352 ||||||||||||||||||||||||||||||||:!!||||||||||||||||||||||||| isGlyValLeuAsnThrAspAsnMetSerIleValGlyLeuThrIleAspTyrGlyProP 58170 : ATGGTGTGCTGAACACAGATAATATGAGCATCGTAGGACTTACTATTGACTATGGCCCTT : 58111 353 : heGlyPheLeuAsp{Ar} >>>> Target Intron 4 >>>> {g}TyrAspProA : 361 ||||||||:!!|||{||} 757 bp {|}|||||||||! heGlyPheMetAsp{Ar}++ ++{g}TyrAspProG 58110 : TTGGGTTTATGGAC{AG}gt.........................ag{A}TATGACCCTG : 57327 362 : spHisValCysAsnAlaSerAspAsnThrGlyArgTyrAlaTyrSerLysGlnProGluV : 381 !:|||:!!||||||!.!|||||||||||||||||||||||||||!:!||||||||||||: luHisIleCysAsnGlySerAspAsnThrGlyArgTyrAlaTyrAsnLysGlnProGluI 57326 : AACACATTTGCAATGGTTCTGATAATACAGGACGCTACGCTTATAACAAGCAGCCAGAAA : 57267 382 : alCysArgTrpAsnLeuArgLysLeuAlaGluAlaLeuGlnProGluLeuProLeuGluL : 401 !!|||!:!||||||||| !!|||||||||||||||||| ||||||||||||||||||: leCysLysTrpAsnLeuGlyLysLeuAlaGluAlaLeuValProGluLeuProLeuGluI 57266 : TTTGCAAATGGAATCTAGGGAAGCTTGCTGAAGCTTTAGTCCCTGAATTGCCCTTGGAGA : 57207 402 : euGlyGluAlaIleLeuAlaGluGluPheAspAlaGluPheGlnArgHisTyrLeuGlnL : 421 !!.!!||| !||||||! !||||||!:!:!!!.!||||||:!!!:!||||||||||||| leSerGluLeuIleLeuGluGluGluTyrAsnValGluPheGluLysHisTyrLeuGlnL 57206 : TCAGCGAACTCATTCTGGAAGAGGAGTACAATGTAGAATTTGAGAAGCATTATTTGCAGA : 57147 422 : ysMetArgArgLysLeuGlyLeuValGlnValGluLeuGluGluAspGlyAlaLeuValS : 441 ||||||||!:!||||||||||||:!!|||:!!|||||||||||||||..! !||||||| ysMetArgLysLysLeuGlyLeuIleGlnLeuGluLeuGluGluAspAsnLysLeuValS 57146 : AGATGAGAAAGAAGCTAGGACTCATTCAGCTGGAATTAGAAGAAGATAATAAATTGGTAT : 57087 442 : erLysLeuLeuGluThrMetHisLeuThr{G} >>>> Target Intron 5 >>>> : 451 ||:!!||||||||||||||||||||||||{|} 227 bp erGluLeuLeuGluThrMetHisLeuThr{G}++ + 57086 : CTGAACTCCTAGAAACTATGCATCTCACA{G}gt.........................a : 56831 452 : {ly}AlaAspPheThrAsnThrPheTyrLeuLeuSerSerPheProValGluLeuGluS : 470 {||}|||||||||||||||! !||| ||||||||||||||| !!|||!!:|||!!:! +{ly}AlaAspPheThrAsnIlePheArgLeuLeuSerSerPheSerValAspLeuAspP 56830 : g{GT}GCAGACTTCACAAATATTTTCCGGTTGCTGAGTTCGTTCTCAGTAGATTTGGATT : 56773 471 : erProGlyLeuAlaGluPheLeuAlaArgLeuMetGluGlnCysAlaSerLeuGluGluL : 490 ! ! !!!!! !|||||||||! !!:!:!! .!.||||||||||||:!!||||||| heLeuLysPheGluGluPheLeuGluLysIleAlaAsnGlnCysAlaSerMetGluGluL 56772 : TTTTAAAATTTGAGGAATTTCTAGAAAAAATTGCCAATCAGTGTGCTTCCATGGAAGAAT : 56713 491 : euArgLeuAlaPheArgProGlnMetAspPro{Ar} >>>> Target Intron 6 > : 501 ||!:!:!!||||||!:!|||||||||||||||{||} 2277 bp euLysValAlaPheLysProGlnMetAspPro{Ar}++ 56712 : TGAAAGTCGCTTTCAAGCCCCAGATGGATCCA{AG}gt...................... : 56676 502 : >>> {g}GlnLeuSerMetMetLeuMetLeuAlaGlnSerAsnProGlnLeuPheAlaL : 519 {|}|||||||||||||||||||||||||||||||||||||||||||||||||||| ++{g}GlnLeuSerMetMetLeuMetLeuAlaGlnSerAsnProGlnLeuPheAlaL 56675 : ...ag{A}CAACTTTCCATGATGCTGATGTTGGCTCAATCTAATCCTCAACTGTTTGCTT : 54349 520 : euMetGlyThrArgAlaGlyIleAlaArgGluLeuGluArgValGluGlnGlnSerArgL : 539 ||!!:||||||!:!|||..!||| !..!||||||||||||:!!|||||| ! |||!:!| euIleGlyThrLysAlaAsnIleAsnGluGluLeuGluArgIleGluGlnTyrSerLysL 54348 : TAATTGGAACAAAAGCTAATATAAATGAAGAATTAGAACGCATAGAACAATATTCTAAAT : 54289 540 : euGluGlnLeuSerAlaAlaGluLeuGlnSerArgAsnGlnGlyHisTrpAlaAspTrpL : 559 ||:!!||||||!!!||||||!!:|||! |||||||||.!. !!||||||.!!!!:|||| euGlnGlnLeuThrAlaAlaAspLeuLeuSerArgAsnAsnArgHisTrpThrGluTrpL 54288 : TGCAGCAGTTAACAGCAGCTGACCTACTCAGCAGAAATAACAGACATTGGACAGAATGGT : 54229 560 : euGlnAlaTyr{Ar} >>>> Target Intron 7 >>>> {g}AlaArgLeuAspL : 568 ||:!! !|||{||} 2567 bp {|}|||||||||!!:| euGluLysTyr{Ar}++ ++{g}AlaArgLeuGluL 54228 : TGGAGAAATAC{AG}gt.........................ag{A}GCCCGCTTAGAAA : 51635 569 : ysAspLeuGluGlyAlaGlyAspAlaAlaAlaTrpGlnAlaGluHisValArgValMetH : 588 ||!!::!!|||..! !..!! !!.!! !.!!|||.!.||||||!.!|||!:!||||||: ysGluIleGluAsnIleAsnAlaValAspThrTrpAsnAlaGluArgValLysValMetA 51634 : AAGAAATAGAAAACATTAATGCTGTTGATACTTGGAATGCTGAACGTGTGAAAGTTATGA : 51575 589 : isAlaAsnAsnProLysTyrValLeuArgAsnTyrIleAlaGlnAsnAlaIleGluAlaA : 608 !!:!!|||||||||||||||:!!||||||||||||||||||||||||||||||||||||| snSerAsnAsnProLysTyrIleLeuArgAsnTyrIleAlaGlnAsnAlaIleGluAlaA 51574 : ATTCAAACAATCCAAAATATATTTTGAGAAACTACATCGCTCAAAATGCCATAGAAGCAG : 51515 609 : laGluArgGlyAspPheSerGlu >>>> Target Intron 8 >>>> ValArgAr : 618 |||||!.!|||||||||!!!||| 559 bp ||||||!: laGluAsnGlyAspPheThrGlu++ ++ValArgLy 51514 : CTGAAAATGGTGACTTCACAGAGgt.........................agGTAAGAAA : 50926 619 : gValLeuLysLeuLeuGluThrProTyr : 627 !|||||||||||||||||| !|||!:! sValLeuLysLeuLeuGluAspProPhe 50925 : GGTGTTGAAGCTGTTGGAGGATCCATTC : 50897 vulgar: SPP00000081_2.0 103 627 . gi|397461378|gb|JH734123.1|:subseq(1630000,100000) 70748 50896 - 2090 M 81 243 S 0 2 5 0 2 I 0 4700 3 0 2 S 1 1 M 67 201 S 0 2 5 0 2 I 0 940 3 0 2 S 1 1 M 60 180 5 0 2 I 0 6241 3 0 2 M 43 129 S 0 2 5 0 2 I 0 753 3 0 2 S 1 1 M 93 279 S 0 1 5 0 2 I 0 223 3 0 2 S 1 2 M 49 147 S 0 2 5 0 2 I 0 2273 3 0 2 S 1 1 M 61 183 S 0 2 5 0 2 I 0 2563 3 0 2 S 1 1 M 52 156 5 0 2 I 0 555 3 0 2 M 12 36 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-15 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local gene 50897 70748 2090 - . gene_id 1 ; sequence SPP00000081_2.0 ; gene_orientation + gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local cds 70504 70748 . - . gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local exon 70504 70748 . - . insertions 0 ; deletions 0 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice5 70502 70503 . - . intron_id 1 ; splice_site "GT" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local intron 65800 70503 . - . intron_id 1 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice3 65800 65801 . - . intron_id 0 ; splice_site "AG" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local cds 65596 65799 . - . gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local exon 65596 65799 . - . insertions 0 ; deletions 0 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice5 65594 65595 . - . intron_id 2 ; splice_site "GT" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local intron 64652 65595 . - . intron_id 2 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice3 64652 64653 . - . intron_id 1 ; splice_site "AG" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local cds 64471 64651 . - . gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local exon 64471 64651 . - . insertions 0 ; deletions 0 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice5 64469 64470 . - . intron_id 3 ; splice_site "GT" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local intron 58226 64470 . - . intron_id 3 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice3 58226 58227 . - . intron_id 2 ; splice_site "AG" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local cds 58095 58225 . - . gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local exon 58095 58225 . - . insertions 0 ; deletions 0 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice5 58093 58094 . - . intron_id 4 ; splice_site "GT" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local intron 57338 58094 . - . intron_id 4 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice3 57338 57339 . - . intron_id 3 ; splice_site "AG" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local cds 57057 57337 . - . gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local exon 57057 57337 . - . insertions 0 ; deletions 0 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice5 57055 57056 . - . intron_id 5 ; splice_site "GT" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local intron 56830 57056 . - . intron_id 5 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice3 56830 56831 . - . intron_id 4 ; splice_site "AG" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local cds 56679 56829 . - . gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local exon 56679 56829 . - . insertions 0 ; deletions 0 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice5 56677 56678 . - . intron_id 6 ; splice_site "GT" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local intron 54402 56678 . - . intron_id 6 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice3 54402 54403 . - . intron_id 5 ; splice_site "AG" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local cds 54216 54401 . - . gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local exon 54216 54401 . - . insertions 0 ; deletions 0 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice5 54214 54215 . - . intron_id 7 ; splice_site "GT" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local intron 51649 54215 . - . intron_id 7 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice3 51649 51650 . - . intron_id 6 ; splice_site "AG" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local cds 51492 51648 . - . gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local exon 51492 51648 . - . insertions 0 ; deletions 0 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice5 51490 51491 . - . intron_id 8 ; splice_site "GT" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local intron 50933 51491 . - . intron_id 8 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local splice3 50933 50934 . - . intron_id 7 ; splice_site "AG" gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local cds 50897 50932 . - . gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local exon 50897 50932 . - . insertions 0 ; deletions 0 gi|397461378|gb|JH734123.1|:subseq(1630000,100000) exonerate:protein2genome:local similarity 50897 70748 2090 - . alignment_id 1 ; Query SPP00000081_2.0 ; Align 70749 104 243 ; Align 65799 186 201 ; Align 64651 254 180 ; Align 58226 314 129 ; Align 57337 358 279 ; Align 56828 452 147 ; Align 54401 502 183 ; Align 51648 564 156 ; Align 50933 616 36 # --- END OF GFF DUMP --- # -- completed exonerate analysis