TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000081_2.0 # Protein # Selenoprotein O (SelO) # Human
(Homo sapiens)
(669 letters)
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g
enome.fa
14,644 sequences; 2,174,243,242 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|397461378|gb|JH734123.1| Alligator mississippiensis unplaced ... 135 7e-30
gi|397463838|gb|JH731663.1| Alligator mississippiensis unplaced ... 54 1e-10
gi|397458860|gb|JH736641.1| Alligator mississippiensis unplaced ... 35 9.3
>gi|397461378|gb|JH734123.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-5035, whole genome shotgun sequence
Length = 1871636
Score = 135 bits (340), Expect = 7e-30
Identities = 61/98 (62%), Positives = 75/98 (76%)
Frame = -2
Query: 354 FLDRYDPDHVCNASDNTGRYAYSKQPEVCRWNLRKXXXXXXXXXXXXXXXXILAEEFDAE 413
F+ RYDP+H+CN SDNTGRYAY+KQPE+C+WNL K IL EE++ E
Sbjct: 1687348 FVCRYDPEHICNGSDNTGRYAYNKQPEICKWNLGKLAEALVPELPLEISELILEEEYNVE 1687169
Query: 414 FQRHYLQKMRRKLGLVQVELEEDGALVSKLLETMHLTG 451
F++HYLQKMR+KLGL+Q+ELEED LVS+LLETMHLTG
Sbjct: 1687168 FEKHYLQKMRKKLGLIQLELEEDNKLVSELLETMHLTG 1687055
Score = 131 bits (329), Expect = 1e-28
Identities = 61/69 (88%), Positives = 67/69 (97%)
Frame = -3
Query: 185 RQADGRKVLRSSIREFLCSEAMFHLGVPTTRAGACVTSESTVVRDVFYDGNPKYEQCTVV 244
RQADGRKVLRSSIREFLCSEAMFHLG+PTTRAG CVTS+S V+RDVFYDGNPK+E+CTVV
Sbjct: 1695801 RQADGRKVLRSSIREFLCSEAMFHLGIPTTRAGTCVTSDSKVIRDVFYDGNPKHERCTVV 1695622
Query: 245 LRVASTFIR 253
LR+ASTFIR
Sbjct: 1695621 LRIASTFIR 1695595
Score = 114 bits (285), Expect(2) = 9e-27
Identities = 53/64 (82%), Positives = 54/64 (84%)
Frame = -3
Query: 122 LFFSGNALLPGAEPAAHCYCGHQFGQFAGQLGDGAAMYLGEVCTATGERWELQLKGAGPT 181
L FSGN L PGAEPAAHCYCGHQFG FAGQLGDGAAMYLGEV G RWE+QLKGAG T
Sbjct: 1700694 LCFSGNRLPPGAEPAAHCYCGHQFGSFAGQLGDGAAMYLGEVLGPAGRRWEVQLKGAGLT 1700515
Query: 182 PFSR 185
PFSR
Sbjct: 1700514 PFSR 1700503
Score = 103 bits (257), Expect = 3e-20
Identities = 49/66 (74%), Positives = 56/66 (84%)
Frame = -2
Query: 253 RFGSFEIFKSADEHTGRAGPSVGRNDIRVQLLDYVISSFYPEIQAAHASDSVQRNAAFFR 312
RFGSFEIFK DEHTGR GPSV RNDIR Q+L+YVIS+FYPEIQ A+ +++QRNAAFFR
Sbjct: 1694653 RFGSFEIFKPMDEHTGRKGPSVNRNDIRTQMLNYVISTFYPEIQQAYPDNTIQRNAAFFR 1694474
Query: 313 EVTRRT 318
EV R T
Sbjct: 1694473 EVGRPT 1694456
Score = 96.3 bits (238), Expect = 4e-18
Identities = 47/72 (65%), Positives = 57/72 (79%)
Frame = -1
Query: 501 RQLSMMLMLAQSNPQLFALMGTRAGIARELERVEQQSRLEQLSAAELQSRNQGHWADWLQ 560
RQLSMMLMLAQSNPQLFAL+GT+A I ELER+EQ S+L+QL+AA+L SRN HW +WL+
Sbjct: 1684403 RQLSMMLMLAQSNPQLFALIGTKANINEELERIEQYSKLQQLTAADLLSRNNRHWTEWLE 1684224
Query: 561 AYRARLDKDLEG 572
YR L +G
Sbjct: 1684223 KYR*DLLNHFQG 1684188
Score = 94.7 bits (234), Expect = 1e-17
Identities = 40/47 (85%), Positives = 46/47 (97%)
Frame = -1
Query: 311 FREVTRRTARMVAEWQCVGFCHGVLNTDNMSILGLTIDYGPFGFLDR 357
F ++T+RTAR+VAEWQCVGFCHGVLNTDNMSI+GLTIDYGPFGF+DR
Sbjct: 1688234 FFQITKRTARLVAEWQCVGFCHGVLNTDNMSIVGLTIDYGPFGFMDR 1688094
Score = 82.8 bits (203), Expect = 5e-14
Identities = 37/56 (66%), Positives = 46/56 (82%)
Frame = -3
Query: 562 YRARLDKDLEGAGDAAAWQAEHVRVMHANNPKYVLRNYIAQNAIEAAERGDFSEVR 617
+RARL+K++E W AE V+VM++NNPKY+LRNYIAQNAIEAAE GDF+EVR
Sbjct: 1681653 FRARLEKEIENINAVDTWNAERVKVMNSNNPKYILRNYIAQNAIEAAENGDFTEVR 1681486
Score = 68.9 bits (167), Expect = 8e-10
Identities = 32/53 (60%), Positives = 41/53 (77%)
Frame = -1
Query: 449 LTGADFTNTFYLLSSFPVELESPGLAEFLARLMEQCASLEELRLAFRPQMDPR 501
+ GADFTN F LLSSF V+L+ EFL ++ QCAS+EEL++AF+PQMDPR
Sbjct: 1686836 ILGADFTNIFRLLSSFSVDLDFLKFEEFLEKIANQCASMEELKVAFKPQMDPR 1686678
Score = 31.6 bits (70), Expect(2) = 9e-27
Identities = 27/78 (34%), Positives = 30/78 (38%), Gaps = 11/78 (14%)
Frame = -1
Query: 28 APRSTLS-GAAMEPAPRWLAGLRFDNRALXXXXXXXXXXXXXXXXXXXXXVPGACFTRVQ 86
AP S+ S G E WL LRFDN AL VPGACF+RV
Sbjct: 1700909 APGSSSSEGRDAEAGAGWLGALRFDNLAL------RSLPLDPCDDPAPRPVPGACFSRVX 1700748
Query: 87 ----------PTPLRQPR 94
P P +PR
Sbjct: 1700747 RCWGCGRRRGPRPRTRPR 1700694
>gi|397463838|gb|JH731663.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-1088, whole genome shotgun sequence
Length = 657272
Score = 53.9 bits (128), Expect(2) = 1e-10
Identities = 24/32 (75%), Positives = 28/32 (87%)
Frame = -3
Query: 185 RQADGRKVLRSSIREFLCSEAMFHLGVPTTRA 216
R DGR VLRSS+REFLCSEAM +LG+PT+RA
Sbjct: 108780 RDGDGRAVLRSSVREFLCSEAMHYLGIPTSRA 108685
Score = 50.8 bits (120), Expect = 2e-04
Identities = 20/28 (71%), Positives = 24/28 (85%)
Frame = -2
Query: 333 GVLNTDNMSILGLTIDYGPFGFLDRYDP 360
GV NTDN S+L +TIDYGPFGF++ YDP
Sbjct: 90535 GVCNTDNFSLLSITIDYGPFGFMESYDP 90452
Score = 37.7 bits (86), Expect(2) = 1e-10
Identities = 15/18 (83%), Positives = 17/18 (94%)
Frame = -1
Query: 168 GERWELQLKGAGPTPFSR 185
GERWELQLKG+G TP+SR
Sbjct: 108926 GERWELQLKGSGKTPYSR 108873
Score = 37.7 bits (86), Expect = 1.9
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = -3
Query: 591 NPKYVLRNYIAQNAIEAAERGDFSEVR 617
NP+YVLRN++A++AI AE DFS V+
Sbjct: 65649 NPRYVLRNWMAESAIRKAEMNDFSGVK 65569
>gi|397458860|gb|JH736641.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-9165, whole genome shotgun sequence
Length = 621014
Score = 35.4 bits (80), Expect = 9.3
Identities = 26/81 (32%), Positives = 35/81 (43%), Gaps = 5/81 (6%)
Frame = +2
Query: 130 LPGAEPAAHCYCGHQFGQFAGQLGDGAAMYLGEV-CTA----TGERWELQLKGAGPTPFS 184
+P + P +C +LG G M+LG+V C A TG++W GA P P S
Sbjct: 496679 IPSSLPGCPLHCPQSPPLAESRLGRGICMWLGQVHCIAASCSTGQKWVRNSYGALPVPVS 496858
Query: 185 RQADGRKVLRSSIREFLCSEA 205
A S E LC E+
Sbjct: 496859 --AGSHTASAGSSWEILCRES 496915
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis
/genome.fa
Posted date: Oct 18, 2013 5:53 PM
Number of letters in database: 2,174,243,242
Number of sequences in database: 14,644
Lambda K H
0.322 0.135 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,616,934,072
Number of Sequences: 14644
Number of extensions: 23980738
Number of successful extensions: 118359
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 575
Number of HSP's successfully gapped in prelim test: 426
Number of HSP's that attempted gapping in prelim test: 111847
Number of HSP's gapped (non-prelim): 21121
length of query: 669
length of database: 724,747,747
effective HSP length: 137
effective length of query: 532
effective length of database: 722,741,519
effective search space: 384498488108
effective search space used: 384498488108
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 80 (35.4 bits)