Command line: [exonerate -m p2g --showtargetgff -q Sel.tortu/SELNTORTU.fa -t gi|397456876|gb|JH738624.1|subseq.fa -E] Hostname: [ws101145] ** (process:2923): WARNING **: Exhaustively generating suboptimal alignments will be VERY SLOW C4 Alignment: ------------ Query: SPP00002135_2.0 # Protein # Selenoprotein N (SelN) # Softshell turtle (Pelodiscus sinensis) Target: gi|397456876|gb|JH738624.1|:subseq(580000,100000) Alligator mississippiensis unplaced genomic scaffold scaffold-12508, whole genome shotgun sequence Model: protein2genome:local Raw score: 2257 Query range: 0 -> 496 Target range: 1122 -> 70900 1 : AsnGln >>>> Target Intron 1 >>>> GluThrAlaLeuLysSerLeuGlyA : 11 |||||| 56468 bp |||||||||||||||:!!||||||| AsnGln++ ++GluThrAlaLeuLysAlaLeuGlyA 1123 : AACCAGgt.........................agGAGACAGCTTTGAAGGCTCTGGGAA : 57621 12 : snGluGlyLeuPheLeuPheSerSerLeuAspThrAspLysAspLeuTyrIleSerProG : 31 ||||||||!!!||||||||||||||||||||||||:!!!!.|||||||||:!:||||||| snGluGlyPhePheLeuPheSerSerLeuAspThrAsnAsnAspLeuTyrLeuSerProG 57622 : ATGAGGGCTTTTTTCTCTTCTCCTCCCTGGACACTAACAATGATCTGTACCTGAGCCCTG : 57681 32 : luGluPheLysProIleAlaGluLysLeuThr{G} >>>> Target Intron 2 >> : 42 ||||||||||||||||||||||||||||||||{|} 841 bp luGluPheLysProIleAlaGluLysLeuThr{G}++ 57682 : AGGAGTTCAAGCCAATAGCTGAGAAGTTAACG{G}gt....................... : 57717 43 : >> {ly}ValThrProValSerAspPheGluGluGluGluThrLeuAspProAsnGlyG : 60 {||}|||||||||||||||!!:!:!||||||||||||||||||||||||!:!|||| ++{ly}ValThrProValSerGluTyrGluGluGluGluThrLeuAspProSerGlyG 57718 : ..ag{GT}GTTACTCCTGTCTCTGAATACGAAGAGGAAGAGACGCTGGATCCCAGTGGGG : 58609 61 : luThrLeuSerIleValAlaLysPheGlnProLeuLeuLeuGluThrMetThrLysSerL : 80 ||! !||||||:!!!.!|||||||||||||||||||||:!!||||||||||||||||||| luMetLeuSerValAlaAlaLysPheGlnProLeuLeuMetGluThrMetThrLysSerL 58610 : AGATGCTGTCTGTCGCTGCTAAATTCCAGCCCTTGCTCATGGAAACGATGACGAAGAGCA : 58669 81 : ysAspGlyPheLeuGly >>>> Target Intron 3 >>>> IleSerHisValAl : 90 ||||||||||||||||| 639 bp |||||||||||||| ysAspGlyPheLeuGly++ ++IleSerHisValAl 58670 : AAGACGGTTTTCTAGGAgt.........................agATTTCTCACGTTGC : 59338 91 : aLeuSerGlyLeuArgAsnTrpThrAlaProAlaThrProValSerValMetSerAlaLy : 110 ||||||||||||||||||||||||||||||||||.!!||||||!:!||||||! !|||!: aLeuSerGlyLeuArgAsnTrpThrAlaProAlaAlaProValAsnValMetPheAlaAr 59339 : TCTGTCTGGGCTCCGGAACTGGACGGCGCCAGCGGCCCCCGTGAACGTGATGTTTGCCAG : 59398 111 : sGlnPheLysAlaPheLeuProProLysAsnLysLeuGluLeuGlyAspProTrpTrpIl : 130 !||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| gGlnPheLysAlaPheLeuProProLysAsnLysLeuGluLeuGlyAspProTrpTrpIl 59399 : GCAGTTCAAAGCTTTTCTCCCTCCAAAGAATAAGCTGGAACTCGGCGATCCCTGGTGGAT : 59458 131 : eIleProSerGluLeuAsnIlePheThrGlyTyrLeuSerAsnAsnArgPheSerLysSe : 150 ||||||||||||||||||||||||||||||||||||||||||||||||||||! ! ! eIleProSerGluLeuAsnIlePheThrGlyTyrLeuSerAsnAsnArgPheTyrProPr 59459 : TATCCCTAGCGAACTGAACATCTTCACCGGGTACCTTTCTAACAACCGGTTTTACCCTCC : 59518 151 : rGlnAspLeuProArgVal >>>> Target Intron 4 >>>> IleLeuIleHis : 160 ! ! ! !!:!! ! 325 bp |||:!!|||||| oAlaProLysGlyLysGlu++ ++IleIleIleHis 59519 : AGCCCCCAAGGGCAAAGAGgt.........................agATCATCATCCAC : 59873 161 : LysLeuLeuSerMetPheHisProArgProPheValLysThrArgPheAlaProGlnGly : 180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| LysLeuLeuSerMetPheHisProArgProPheValLysThrArgPheAlaProGlnGly 59874 : AAACTCTTGAGCATGTTCCATCCACGCCCGTTTGTAAAGACCCGCTTTGCGCCTCAAGGC : 59933 181 : AlaValAlaCysValGlnAlaIleSerGluPheTyrTyrLeuIleAlaLeu{Ar} >>> : 198 ||||||||||||:!!||||||! !||||||||||||||| !:!!|||!!!{||} AlaValAlaCysIleGlnAlaSerSerGluPheTyrTyrThrValAlaPhe{Ar}++ 59934 : GCTGTGGCCTGCATCCAGGCAAGCAGCGAGTTTTACTATACCGTCGCATTC{AG}gt... : 59991 199 : > Target Intron 5 >>>> {g}IleHisAlaGluPheGlnLeuAsnGluProPro : 209 1222 bp {|}||||||||||||||||||||||||||||||||| ++{g}IleHisAlaGluPheGlnLeuAsnGluProPro 59992 : ......................ag{G}ATTCATGCTGAGTTCCAGTTGAATGAGCCTCCA : 61242 210 : AspPheProPheTrpPheSerProAlaGlnPheThrGlyHisIleValLeuSerArgAsp : 229 ||||||||||||||||||||||||!.!|||||||||||||||:!!:!!||||||!:!||| AspPheProPheTrpPheSerProGlyGlnPheThrGlyHisValIleLeuSerLysAsp 61243 : GATTTCCCTTTCTGGTTCTCCCCTGGCCAGTTCACAGGACACGTCATCCTCTCCAAAGAT : 61302 230 : SerSerHisValArgGluPheLysLeuPheValProAsnLys{Ar} >>>> Target : 244 |||||||||||||||||||||||||||||||||||||||!!.{||} 224 SerSerHisValArgGluPheLysLeuPheValProAsnAsn{Ar}++ 61303 : TCTTCCCATGTTCGGGAGTTCAAGCTCTTTGTCCCAAACAAC{AG}gt............ : 61351 245 : Intron 6 >>>> {g}SerLeuAsnValAspMetGluTrpLeuTyrGlyAlaSerGlu : 258 3 bp {|}|||||||||||||||||||||||||||||||||||||||||| ++{g}SerLeuAsnValAspMetGluTrpLeuTyrGlyAlaSerGlu 61352 : .............ag{G}TCCCTAAACGTTGACATGGAATGGCTATACGGGGCGAGTGAA : 63632 259 : SerSerSerMetGluValAspIleGlyTyrLeuProGln >>>> Target Intron : 272 ||||||!:!|||||||||||||||||||||||||||||| 1041 bp SerSerAsnMetGluValAspIleGlyTyrLeuProGln++ 63633 : AGCAGCAACATGGAGGTGGACATTGGTTACCTCCCGCAGgt................... : 63676 273 : 7 >>>> MetGluLeuGluSerSerGlyProSerIleProSerValIleHisAspGluA : 289 |||||||||||||||! !|||||||||||||||||||||||||||||||||| ++MetGluLeuGluSerMetGlyProSerIleProSerValIleHisAspGluA 63677 : ......agATGGAGTTAGAATCGATGGGGCCATCCATCCCTTCTGTGATTCACGATGAAA : 64766 290 : snGlyAsnValLeuAspSerArgAspProAlaGlyGluProValGlnPheValPheGluG : 309 |||||||||||:!!|||||||||||||||.!!|||||||||:!!|||||||||||||||| snGlyAsnValIleAspSerArgAspProThrGlyGluProIleGlnPheValPheGluG 64767 : ATGGAAATGTGATTGACAGCAGAGACCCCACGGGGGAGCCCATCCAGTTTGTGTTTGAAG : 64826 310 : luIleArgTrpGlnGlnGluMetSerTrpGluGluAlaAlaGlnLysLeuGluValAlaM : 329 |||||! !|||||||||:!!!!:!!!|||||||||||||||||||||||||||||||||| luIleThrTrpGlnGlnLysIleThrTrpGluGluAlaAlaGlnLysLeuGluValAlaM 64827 : AGATAACCTGGCAGCAGAAAATCACTTGGGAGGAAGCGGCTCAGAAGCTGGAAGTTGCCA : 64886 330 : etTyrProPheLysLys >>>> Target Intron 8 >>>> ValSerTyrLeuPr : 339 ||||||||||||||||| 1465 bp |||||||||||||| etTyrProPheLysLys++ ++ValSerTyrLeuPr 64887 : TGTATCCGTTTAAGAAAgt.........................agGTTTCGTACCTGCC : 66381 340 : oPheThrGlnAlaPheGluArgAlaLysAlaGluLysLysLeuValHisSerIleLeuLe : 359 ||||||||||||||||||||||||||||||||||!!.||||||||||||||||||||||| oPheThrGlnAlaPheGluArgAlaLysAlaGluAsnLysLeuValHisSerIleLeuLe 66382 : CTTTACACAAGCCTTTGAGAGAGCAAAAGCTGAGAACAAGCTGGTGCACTCCATCCTGCT : 66441 360 : uTrpGlyAlaLeuAspAspGlnSerCysUnk{G} >>>> Target Intron 9 >>> : 370 |||||||||||||||||||||||||||| {|} 498 bp uTrpGlyAlaLeuAspAspGlnSerCys***{G}++ 66442 : GTGGGGCGCGCTGGACGACCAGTCCTGCTGA{G}gt........................ : 66477 371 : > {ly}SerGlyArgThrLeuArgGluThrValLeuGluSerSerProValLeuAlaLe : 388 {||}||||||||||||||||||||||||||||||||||||||||||:!!|||||||| ++{ly}SerGlyArgThrLeuArgGluThrValLeuGluSerSerProIleLeuAlaLe 66478 : .ag{GT}TCGGGGCGGACTCTCCGGGAGACCGTCCTGGAAAGTTCGCCCATCCTCGCTTT : 67026 389 : uLeuAsnGluSerPheValSerSerTrpSerLeuValLysGluLeuGluGluLeuGln : 408 |||||||||||||||||||||||||||||||||||||||||||||||||!!:|||||| uLeuAsnGluSerPheValSerSerTrpSerLeuValLysGluLeuGluAspLeuGln++ 67027 : GCTGAATGAGAGCTTTGTCAGCAGCTGGTCACTAGTGAAGGAGCTAGAGGACTTGCAGgt : 67086 409 : >>>> Target Intron 10 >>>> AlaArgArgGlnAspLysPheSerSerGlnLe : 418 1368 bp .!!!:!|||:!!:!!:!!|||! !!!!:!!|| ++ThrLysArgGluAsnGluPheTyrArgLysLe 67087 : ..........................agACCAAGAGAGAGAATGAATTCTACAGGAAGCT : 68484 419 : uAlaAlaLeuHisLeuGluLysTyrAsnPheProValGluMetMetIleCysLeuProAs : 438 ||||! !||||||||||||||||||||||||||||||||||||||||||||||||||||| uAlaGluLeuHisLeuGluLysTyrAsnPheProValGluMetMetIleCysLeuProAs 68485 : AGCGGAGCTGCACCTAGAGAAATACAACTTCCCTGTGGAGATGATGATCTGCCTTCCCAA : 68544 439 : nGlyThrVal >>>> Target Intron 11 >>>> IleHisHisIleAsnAlaAs : 448 |||||||||| 2180 bp |||||||||||||||||||| nGlyThrVal++ ++IleHisHisIleAsnAlaAs 68545 : TGGAACAGTGgt..........................agATTCACCACATCAATGCCAA : 70754 449 : nTyrPheLeuAspIleThrSerMetLysProGluGluValGluSerSerIlePheSerPh : 468 |||||||||||||||||||||||||||||||||||||||||||||||||:!!|||||||| nTyrPheLeuAspIleThrSerMetLysProGluGluValGluSerSerValPheSerPh 70755 : CTACTTCCTGGATATTACTTCTATGAAGCCCGAAGAAGTTGAGAGCAGCGTCTTCAGTTT : 70814 469 : eSerSerAsnPheGluAspProSerThrAlaThrTyrLeuGlnPheLeuLysGluGlyLe : 488 ||||!!!|||||||||||||||||||||||||||||||||!:!||||||||||||||||| eSerThrAsnPheGluAspProSerThrAlaThrTyrLeuArgPheLeuLysGluGlyLe 70815 : CTCAACCAATTTTGAAGATCCTTCTACTGCGACCTACCTCCGGTTCCTGAAGGAGGGACT : 70874 489 : uGlnArgAlaLysProPheLeuGln : 496 |!!.||||||||||||||||||||| uHisArgAlaLysProPheLeuGln 70875 : GCACAGAGCAAAACCCTTCTTGCAG : 70900 vulgar: SPP00002135_2.0 0 496 . gi|397456876|gb|JH738624.1|:subseq(580000,100000) 1122 70900 + 2257 M 2 6 5 0 2 I 0 56464 3 0 2 M 39 117 S 0 1 5 0 2 I 0 837 3 0 2 S 1 2 M 43 129 5 0 2 I 0 635 3 0 2 M 71 213 5 0 2 I 0 321 3 0 2 M 41 123 S 0 2 5 0 2 I 0 1218 3 0 2 S 1 1 M 45 135 S 0 2 5 0 2 I 0 2239 3 0 2 S 1 1 M 27 81 5 0 2 I 0 1037 3 0 2 M 63 189 5 0 2 I 0 1461 3 0 2 M 35 105 S 0 1 5 0 2 I 0 494 3 0 2 S 1 2 M 37 111 5 0 2 I 0 1364 3 0 2 M 34 102 5 0 2 I 0 2176 3 0 2 M 55 165 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-21 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local gene 1123 70900 2257 + . gene_id 1 ; sequence SPP00002135_2.0 ; gene_orientation + gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local cds 1123 1128 . + . gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local exon 1123 1128 . + . insertions 0 ; deletions 0 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice5 1129 1130 . + . intron_id 1 ; splice_site "GT" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local intron 1129 57596 . + . intron_id 1 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice3 57595 57596 . + . intron_id 0 ; splice_site "AG" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local cds 57597 57714 . + . gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local exon 57597 57714 . + . insertions 0 ; deletions 0 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice5 57715 57716 . + . intron_id 2 ; splice_site "GT" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local intron 57715 58555 . + . intron_id 2 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice3 58554 58555 . + . intron_id 1 ; splice_site "AG" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local cds 58556 58686 . + . gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local exon 58556 58686 . + . insertions 0 ; deletions 0 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice5 58687 58688 . + . intron_id 3 ; splice_site "GT" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local intron 58687 59325 . + . intron_id 3 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice3 59324 59325 . + . intron_id 2 ; splice_site "AG" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local cds 59326 59538 . + . gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local exon 59326 59538 . + . insertions 0 ; deletions 0 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice5 59539 59540 . + . intron_id 4 ; splice_site "GT" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local intron 59539 59863 . + . intron_id 4 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice3 59862 59863 . + . intron_id 3 ; splice_site "AG" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local cds 59864 59988 . + . gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local exon 59864 59988 . + . insertions 0 ; deletions 0 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice5 59989 59990 . + . intron_id 5 ; splice_site "GT" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local intron 59989 61210 . + . intron_id 5 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice3 61209 61210 . + . intron_id 4 ; splice_site "AG" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local cds 61211 61348 . + . gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local exon 61211 61348 . + . insertions 0 ; deletions 0 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice5 61349 61350 . + . intron_id 6 ; splice_site "GT" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local intron 61349 63591 . + . intron_id 6 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice3 63590 63591 . + . intron_id 5 ; splice_site "AG" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local cds 63592 63673 . + . gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local exon 63592 63673 . + . insertions 0 ; deletions 0 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice5 63674 63675 . + . intron_id 7 ; splice_site "GT" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local intron 63674 64714 . + . intron_id 7 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice3 64713 64714 . + . intron_id 6 ; splice_site "AG" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local cds 64715 64903 . + . gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local exon 64715 64903 . + . insertions 0 ; deletions 0 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice5 64904 64905 . + . intron_id 8 ; splice_site "GT" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local intron 64904 66368 . + . intron_id 8 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice3 66367 66368 . + . intron_id 7 ; splice_site "AG" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local cds 66369 66474 . + . gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local exon 66369 66474 . + . insertions 0 ; deletions 0 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice5 66475 66476 . + . intron_id 9 ; splice_site "GT" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local intron 66475 66972 . + . intron_id 9 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice3 66971 66972 . + . intron_id 8 ; splice_site "AG" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local cds 66973 67085 . + . gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local exon 66973 67085 . + . insertions 0 ; deletions 0 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice5 67086 67087 . + . intron_id 10 ; splice_site "GT" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local intron 67086 68453 . + . intron_id 10 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice3 68452 68453 . + . intron_id 9 ; splice_site "AG" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local cds 68454 68555 . + . gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local exon 68454 68555 . + . insertions 0 ; deletions 0 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice5 68556 68557 . + . intron_id 11 ; splice_site "GT" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local intron 68556 70735 . + . intron_id 11 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local splice3 70734 70735 . + . intron_id 10 ; splice_site "AG" gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local cds 70736 70900 . + . gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local exon 70736 70900 . + . insertions 0 ; deletions 0 gi|397456876|gb|JH738624.1|:subseq(580000,100000) exonerate:protein2genome:local similarity 1123 70900 2257 + . alignment_id 1 ; Query SPP00002135_2.0 ; Align 1123 1 6 ; Align 57597 3 117 ; Align 58558 43 129 ; Align 59326 86 213 ; Align 59864 157 123 ; Align 61212 199 135 ; Align 63593 245 81 ; Align 64715 272 189 ; Align 66369 335 105 ; Align 66975 371 111 ; Align 68454 408 102 ; Align 70736 442 165 # --- END OF GFF DUMP --- # -- completed exonerate analysis