TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00002135_2.0 # Protein # Selenoprotein N (SelN) # Softshell
turtle (Pelodiscus sinensis)
         (496 letters)

Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g
enome.fa 
           14,644 sequences; 2,174,243,242 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|397456876|gb|JH738624.1| Alligator mississippiensis unplaced ...   127   2e-27

>gi|397456876|gb|JH738624.1| Alligator mississippiensis unplaced genomic
              scaffold scaffold-12508, whole genome shotgun sequence
          Length = 927267

 Score =  127 bits (318), Expect = 2e-27
 Identities = 59/64 (92%), Positives = 61/64 (95%)
 Frame = +2

Query: 84     LGISHVALSGLRNWTAPATPVSVMSAKQFKAFLPPKNKLELGDPWWIIPSELNIFTGYLS 143
              L ISHVALSGLRNWTAPA PV+VM A+QFKAFLPPKNKLELGDPWWIIPSELNIFTGYLS
Sbjct: 639320 LQISHVALSGLRNWTAPAAPVNVMFARQFKAFLPPKNKLELGDPWWIIPSELNIFTGYLS 639499

Query: 144    NNRF 147
              NNRF
Sbjct: 639500 NNRF 639511



 Score =  125 bits (315), Expect = 4e-27
 Identities = 58/68 (85%), Positives = 63/68 (92%)
 Frame = +3

Query: 272    MELESSGPSIPSVIHDENGNVLDSRDPAGEPVQFVFEEIRWQQEMSWEEAAQKLEVAMYP 331
              MELES GPSIPSVIHDENGNV+DSRDP GEP+QFVFEEI WQQ+++WEEAAQKLEVAMYP
Sbjct: 644715 MELESMGPSIPSVIHDENGNVIDSRDPTGEPIQFVFEEITWQQKITWEEAAQKLEVAMYP 644894

Query: 332    FKKVSYLP 339
              FKKVS  P
Sbjct: 644895 FKKVSKAP 644918



 Score = 98.6 bits (244), Expect = 6e-19
 Identities = 42/47 (89%), Positives = 45/47 (95%)
 Frame = +1

Query: 198    RIHAEFQLNEPPDFPFWFSPAQFTGHIVLSRDSSHVREFKLFVPNKR 244
              RIHAEFQLNEPPDFPFWFSP QFTGH++LS+DSSHVREFKLFVPN R
Sbjct: 641209 RIHAEFQLNEPPDFPFWFSPGQFTGHVILSKDSSHVREFKLFVPNNR 641349



 Score = 83.6 bits (205), Expect = 2e-14
 Identities = 38/46 (82%), Positives = 44/46 (95%)
 Frame = +2

Query: 42     GVTPVSDFEEEETLDPNGETLSIVAKFQPLLLETMTKSKDGFLGIS 87
              GVTPVS++EEEETLDP+GE LS+ AKFQPLL+ETMTKSKDGFLG+S
Sbjct: 638555 GVTPVSEYEEEETLDPSGEMLSVAAKFQPLLMETMTKSKDGFLGVS 638692



 Score = 80.5 bits (197), Expect = 2e-13
 Identities = 36/42 (85%), Positives = 39/42 (92%)
 Frame = +3

Query: 157    ILIHKLLSMFHPRPFVKTRFAPQGAVACVQAISEFYYLIALR 198
              I+IHKLLSMFHPRPFVKTRFAPQGAVAC+QA SEFYY +A R
Sbjct: 639864 IIIHKLLSMFHPRPFVKTRFAPQGAVACIQASSEFYYTVAFR 639989



 Score = 76.6 bits (187), Expect = 2e-12
 Identities = 38/55 (69%), Positives = 39/55 (70%)
 Frame = +3

Query: 442    IHHINANYFLDITSMKPXXXXXXXXXXXXXXXDPSTATYLQFLKEGLQRAKPFLQ 496
              IHHINANYFLDITSMKP               DPSTATYL+FLKEGL RAKPFLQ
Sbjct: 650736 IHHINANYFLDITSMKPEEVESSVFSFSTNFEDPSTATYLRFLKEGLHRAKPFLQ 650900



 Score = 74.7 bits (182), Expect = 9e-12
 Identities = 35/40 (87%), Positives = 38/40 (95%)
 Frame = +1

Query: 3      ETALKSLGNEGLFLFSSLDTDKDLYISPEEFKPIAEKLTG 42
              ETALK+LGNEG FLFSSLDT+ DLY+SPEEFKPIAEKLTG
Sbjct: 637597 ETALKALGNEGFFLFSSLDTNNDLYLSPEEFKPIAEKLTG 637716



 Score = 73.9 bits (180), Expect = 2e-11
 Identities = 37/41 (90%), Positives = 39/41 (95%)
 Frame = +1

Query: 367    SCXGSGRTLRETVLESSPVLALLNESFVSSWSLVKELEELQ 407
              S  GSGRTLRETVLESSP+LALLNESFVSSWSLVKELE+LQ
Sbjct: 646963 SLAGSGRTLRETVLESSPILALLNESFVSSWSLVKELEDLQ 647085



 Score = 72.8 bits (177), Expect = 4e-11
 Identities = 35/40 (87%), Positives = 36/40 (90%)
 Frame = +1

Query: 331    PFKKVSYLPFTQAFERAKAEKKLVHSILLWGALDDQSCXG 370
              P  +VSYLPFTQAFERAKAE KLVHSILLWGALDDQSC G
Sbjct: 646357 PCFQVSYLPFTQAFERAKAENKLVHSILLWGALDDQSC*G 646476



 Score = 62.4 bits (150), Expect = 5e-08
 Identities = 27/37 (72%), Positives = 33/37 (89%)
 Frame = +1

Query: 406    LQARRQDKFSSQLAALHLEKYNFPVEMMICLPNGTVI 442
              LQ +R+++F  +LA LHLEKYNFPVEMMICLPNGTV+
Sbjct: 648448 LQTKRENEFYRKLAELHLEKYNFPVEMMICLPNGTVV 648558



 Score = 61.6 bits (148), Expect = 8e-08
 Identities = 28/35 (80%), Positives = 32/35 (91%)
 Frame = +3

Query: 238    LFVPNKRSLNVDMEWLYGASESSSMEVDIGYLPQM 272
              + V + RSLNVDMEWLYGASESS+MEVDIGYLPQ+
Sbjct: 643572 VLVSSSRSLNVDMEWLYGASESSNMEVDIGYLPQV 643676


  Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis
  /genome.fa
    Posted date:  Oct 18, 2013  5:53 PM
  Number of letters in database: 2,174,243,242
  Number of sequences in database:  14,644
  
Lambda     K      H
   0.318    0.134    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,293,974,864
Number of Sequences: 14644
Number of extensions: 20382497
Number of successful extensions: 78502
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 503
Number of HSP's successfully gapped in prelim test: 424
Number of HSP's that attempted gapping in prelim test: 74644
Number of HSP's gapped (non-prelim): 11759
length of query: 496
length of database: 724,747,747
effective HSP length: 134
effective length of query: 362
effective length of database: 722,785,451
effective search space: 261648333262
effective search space used: 261648333262
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 78 (34.7 bits)