TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00002131_2.0 # Protein # Selenoprotein K (SelK) # Softshell turtle (Pelodiscus sinensis) (94 letters) Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g enome.fa 14,644 sequences; 2,174,243,242 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|397459294|gb|JH736207.1| Alligator mississippiensis unplaced ... 55 5e-07 gi|397460884|gb|JH734617.1| Alligator mississippiensis unplaced ... 32 4.8 >gi|397459294|gb|JH736207.1| Alligator mississippiensis unplaced genomic scaffold scaffold-8455, whole genome shotgun sequence Length = 104849 Score = 55.1 bits (131), Expect = 5e-07 Identities = 25/30 (83%), Positives = 26/30 (86%) Frame = +3 Query: 7 GQVLDTQSRAPWSLSFITDFFWGIADFVVL 36 GQVL Q+RAPWSLS ITDFFW IADFVVL Sbjct: 55098 GQVLGNQNRAPWSLSSITDFFWAIADFVVL 55187 Score = 43.1 bits (100), Expect = 0.002 Identities = 19/28 (67%), Positives = 24/28 (85%) Frame = +1 Query: 34 VVLFFQSIVQPNLRRRGYTSSSNSRYDD 61 ++ FQSI+QP+LRR+G TSSS SRYDD Sbjct: 56686 IISSFQSIIQPDLRRKGNTSSSYSRYDD 56769 >gi|397460884|gb|JH734617.1| Alligator mississippiensis unplaced genomic scaffold scaffold-5851, whole genome shotgun sequence Length = 11861 Score = 32.0 bits (71), Expect = 4.8 Identities = 14/29 (48%), Positives = 19/29 (65%) Frame = +2 Query: 2 VYISNGQVLDTQSRAPWSLSFITDFFWGI 30 V+ S Q+L T +RAP S IT+F WG+ Sbjct: 1814 VHFSGNQILFTVARAPTQKSGITEFSWGV 1900 Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis /genome.fa Posted date: Oct 18, 2013 5:53 PM Number of letters in database: 2,174,243,242 Number of sequences in database: 14,644 Lambda K H 0.326 0.138 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 174,401,757 Number of Sequences: 14644 Number of extensions: 1751739 Number of successful extensions: 10317 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 279 Number of HSP's successfully gapped in prelim test: 76 Number of HSP's that attempted gapping in prelim test: 9847 Number of HSP's gapped (non-prelim): 963 length of query: 94 length of database: 724,747,747 effective HSP length: 69 effective length of query: 25 effective length of database: 723,737,311 effective search space: 18093432775 effective search space used: 18093432775 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 68 (30.8 bits)