TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000076_2.0 # Protein # Selenoprotein K (SelK) # Human (Homo sapiens) (94 letters) Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g enome.fa 14,644 sequences; 2,174,243,242 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|397459294|gb|JH736207.1| Alligator mississippiensis unplaced ... 48 8e-05 >gi|397459294|gb|JH736207.1| Alligator mississippiensis unplaced genomic scaffold scaffold-8455, whole genome shotgun sequence Length = 104849 Score = 47.8 bits (112), Expect = 8e-05 Identities = 20/30 (66%), Positives = 26/30 (86%) Frame = +3 Query: 7 GQVLDSRSQSPWRLSLITDFFWGIAEFVVL 36 GQVL +++++PW LS ITDFFW IA+FVVL Sbjct: 55098 GQVLGNQNRAPWSLSSITDFFWAIADFVVL 55187 Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis /genome.fa Posted date: Oct 18, 2013 5:53 PM Number of letters in database: 2,174,243,242 Number of sequences in database: 14,644 Lambda K H 0.325 0.138 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 169,721,582 Number of Sequences: 14644 Number of extensions: 1556611 Number of successful extensions: 11302 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 322 Number of HSP's successfully gapped in prelim test: 64 Number of HSP's that attempted gapping in prelim test: 10757 Number of HSP's gapped (non-prelim): 1145 length of query: 94 length of database: 724,747,747 effective HSP length: 69 effective length of query: 25 effective length of database: 723,737,311 effective search space: 18093432775 effective search space used: 18093432775 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 68 (30.8 bits)