Command line: [exonerate -m p2g --showtargetgff -q ../Sel.human/SELI.fa -t gi|397463253|gb|JH732248.1|subseq.fa] Hostname: [ws101062] C4 Alignment: ------------ Query: SPP00000074_2.0 # Protein # Selenoprotein I (SelI) # Human (Homo sapiens) Target: gi|397463253|gb|JH732248.1|:subseq(0,80000) Alligator mississippiensis unplaced genomic scaffold scaffold-1979, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 1582 Query range: 18 -> 397 Target range: 44683 -> 22986 19 : LysTyrSerAlaValAspThrAsnProLeuSerLeuTyrValMetHisProPheTrpAsn : 38 :!!|||||||||||||||!:!||||||||||||||||||||||||||||||||||||||| GlnTyrSerAlaValAspSerAsnProLeuSerLeuTyrValMetHisProPheTrpAsn 44683 : CAGTACAGTGCCGTGGACAGCAACCCGCTCTCCCTGTACGTCATGCATCCGTTCTGGAAC : 44626 39 : ThrIleValLys >>>> Target Intron 1 >>>> ValPheProThrTrpLeuA : 49 |||:!:|||||| 331 bp :!!|||||||||||||||| ThrValValLys++ ++IlePheProThrTrpLeuA 44625 : ACGGTGGTGAAGgt.........................agATTTTCCCCACTTGGCTGG : 44262 50 : laProAsnLeuIleThrPheSerGlyPheLeuLeuValValPheAsnPheLeuLeuMetA : 69 ||||||||||||||||||||!!!||||||||||||:!!||||||||||||!!!||||||| laProAsnLeuIleThrPheCysGlyPheLeuLeuLeuValPheAsnPhePheLeuMetA 44261 : CCCCAAATCTGATAACCTTTTGTGGCTTCCTTCTGCTTGTCTTCAACTTCTTCCTCATGG : 44202 70 : laTyrPheAspProAspPheTyrAlaSer{A} >>>> Target Intron 2 >>>> : 79 |||||||||||||||||||||||||||||{|} 7707 bp laTyrPheAspProAspPheTyrAlaSer{A}++ + 44201 : CATACTTTGACCCCGATTTTTATGCTTCT{G}gt.........................a : 36466 80 : {la}ProGlyHisLysHisValProAspTrpValTrpIleValValGlyIleLeuAsnP : 98 {||}|||! !||| !|||||||||:!! ! :!!||||||:!!||||||:!!||||||| +{la}ProAspHisValHisValProAsnArgIleTrpIleMetValGlyLeuLeuAsnP 36465 : g{CA}CCTGATCATGTGCACGTTCCAAATCGAATATGGATCATGGTGGGTCTCCTCAACT : 36408 99 : heValAlaTyrThrLeu{A} >>>> Target Intron 3 >>>> {sp}GlyValA : 107 ||:!!||||||||||||{|} 592 bp {||}||||||| heMetAlaTyrThrLeu{A}++ ++{sp}GlyValA 36407 : TCATGGCATATACGCTA{G}gt.........................ag{AT}GGTGTCG : 35789 108 : spGlyLysGlnAlaArgArgThrAsnSerSerThrProLeuGlyGluLeuPheAspHisG : 127 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| spGlyLysGlnAlaArgArgThrAsnSerSerThrProLeuGlyGluLeuPheAspHisG 35788 : ATGGGAAGCAAGCTCGCCGAACCAACTCCAGCACCCCACTGGGTGAGCTCTTTGACCACG : 35729 128 : lyLeuAspSerTrpSerCysValTyrPheValValThrValTyrSerIlePheGlyArgG : 147 ||||||||||||||:!!|||:!!||||||||||||||||||||||||! !|||||||||| lyLeuAspSerTrpAlaCysMetTyrPheValValThrValTyrSerThrPheGlyArgG 35728 : GCCTGGACAGCTGGGCCTGCATGTACTTCGTCGTGACTGTTTACTCCACCTTCGGCCGGG : 35669 148 : lySerThrGlyValSerValPheValLeuTyrLeuLeuLeuTrpValValLeuPheSerP : 167 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| lySerThrGlyValSerValPheValLeuTyrLeuLeuLeuTrpValValLeuPheSerP 35668 : GATCCACTGGAGTCAGCGTCTTCGTTCTCTATCTCCTTTTATGGGTGGTTTTATTTTCAT : 35609 168 : heIleLeuSerHisTrpGluLysTyrAsnThrGlyIleLeuPheLeuProTrpGlyTyrA : 187 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| heIleLeuSerHisTrpGluLysTyrAsnThrGlyIleLeuPheLeuProTrpGlyTyrA 35608 : TCATCCTCTCCCACTGGGAGAAGTATAACACAGGGATTCTCTTCCTGCCCTGGGGATATG : 35549 188 : spIleSerGlnVal >>>> Target Intron 4 >>>> ThrIleSerPheValTy : 197 |||||||||||:!! 1946 bp |||||||||.!!||||| spIleSerGlnMet++ ++ThrIleSerIleValTy 35548 : ACATCAGCCAGATGgt.........................agACCATTTCCATTGTCTA : 33573 198 : rIleValThrAlaValValGlyValGluAlaTrpTyrGluProPheLeuPheAsnPheLe : 217 |||||||||||||:!!||||||||||||||||||:!!! !|||||||||||||||||||| rIleValThrAlaIleValGlyValGluAlaTrpHisAlaProPheLeuPheAsnPheLe 33572 : CATAGTGACAGCGATTGTGGGAGTTGAGGCCTGGCACGCGCCTTTCCTGTTTAATTTCTT : 33513 218 : uTyrArgAspLeuPheThrAlaMetIleIle{G} >>>> Target Intron 5 >>> : 228 |||||||||||||||||||.!!||||||:!!{|} 3191 bp uTyrArgAspLeuPheThrThrMetIleLeu{G}++ 33512 : ATATAGAGACCTATTCACTACAATGATTCTT{G}gt........................ : 33477 229 : > {ly}CysAlaLeuCysValThrLeuProMetSerLeuLeuAsnPhePhe{Ar} >> : 244 {||}||||||||| ||||||||||||||||||:!!! !||||||!:!{!:} ++{ly}CysAlaLeuThrValThrLeuProMetSerValTyrAsnPheTyr{Ly}++ 33476 : .ag{GT}TGTGCACTCACAGTGACTCTGCCAATGAGCGTGTACAACTTTTAC{AA}gt.. : 30237 245 : >> Target Intron 6 >>>> {g}SerTyrLysAsnAsnThrLeuLysLeuAsnSe : 255 1908 bp {!}:!!||||||!:!||||||||||||! !:!!|| ++{s}AlaTyrLysSerAsnThrLeuLysHisHisSe 30236 : .......................ag{G}GCCTATAAAAGTAACACCCTGAAGCACCACTC : 28300 256 : rValTyrGluAlaMetValProLeuPheSerProCysLeuLeuPheIleLeuSerThrAl : 275 |:!!|||||| !|||:!!|||!!!.!!|||||| ||||||||| !|||!!!|||.! rLeuTyrGluIleMetLeuProPheLeuSerProValLeuLeuPheAlaLeuCysThrTh 28299 : ACTCTATGAAATAATGCTGCCATTTCTCTCACCGGTGTTACTGTTTGCTCTGTGCACTAC : 28240 276 : aTrpIleLeuTrpSerProSerAspIleLeuGluLeuHisProArgValPheTyrPheMe : 295 !||||||!!! !|||||||||||||||||||||:!!|||||||||:!!||||||||||| rTrpIlePheValSerProSerAspIleLeuGluValHisProArgLeuPheTyrPheMe 28239 : CTGGATTTTCGTGTCGCCATCGGACATCCTGGAGGTCCACCCTCGGCTCTTCTACTTCAT : 28180 296 : tValGlyThrAlaPheAlaAsnSerThr >>>> Target Intron 7 >>>> Cys : 305 ||||||||||||||||||||||! !:!! 624 bp ||| tValGlyThrAlaPheAlaAsnIleSer++ ++Cys 28179 : GGTTGGAACAGCCTTTGCTAACATTTCAgt.........................agTGC : 27526 306 : GlnLeuIleValCysGlnMetSerSerThrArgCysProThrLeuAsnTrpLeuLeuVal : 325 ||||||||||||||||||||||||!:!|||||||||! ! !!|||||||||:!!|||:!! GlnLeuIleValCysGlnMetSerAsnThrArgCysGlnProLeuAsnTrpMetLeuLeu 27525 : CAGCTGATTGTGTGTCAGATGAGCAACACCCGATGCCAGCCTCTGAACTGGATGCTGCTG : 27466 326 : ProLeuPheLeuValValLeuValValAsnLeuGlyValAlaSerTyrValGluSerIle : 345 |||||| :!!||||||!!!|||||| !! !|||:!!||| !! !! !|||!!!||| ProLeuAlaValValValPheValValLeuTyrGlyLeuAlaProAsnAsnGluThrIle 27465 : CCACTAGCGGTGGTTGTTTTCGTGGTGCTCTATGGATTAGCACCAAATAATGAAACAATT : 27406 346 : LeuLeuTyrThrLeuThrThrAlaPheThrLeuAlaHisIleHisTyrGlyValArgVal : 365 ||||||||||||||||||.!!!.!.!!|||:!!||||||||||||||||||||| !!||| LeuLeuTyrThrLeuThrAlaValIleThrValAlaHisIleHisTyrGlyValCysVal 27405 : CTCCTGTACACATTAACAGCAGTCATCACCGTGGCGCACATTCACTACGGAGTGTGTGTG : 27346 366 : >>>> Target Intron 8 >>>> ValLysGlnLeuSerSerHisPheGlnIleT : 376 4261 bp |||!!.|||||||||!.!||||||.!.||| ++ ++ValAsnGlnLeuSerLysHisPheAsnIleA 27345 : gt.........................agGTGAACCAGCTAAGCAAGCATTTCAATATCC : 23052 377 : yrProPheSerLeuArgLysProAsnSerAspUnkLeuGlyMetGluGluLysAsnIleG : 396 ||||||||||||!:!|||! !!:!!!!||| |||! !:!!||||||:!!!!.|||| rgProPheSerLeuLysLysArgSerThrAsp***LeuGluValGluGluGlnLysIleG 23051 : GACCCTTCTCACTAAAGAAGCGCAGTACAGATTGACTAGAAGTGGAAGAACAGAAAATCG : 22992 397 : lyLeu : 397 ||||| lyLeu 22991 : GCCTG : 22987 vulgar: SPP00000074_2.0 18 397 . gi|397463253|gb|JH732248.1|:subseq(0,80000) 44683 22986 - 1582 M 24 72 5 0 2 I 0 327 3 0 2 M 36 108 S 0 1 5 0 2 I 0 7703 3 0 2 S 1 2 M 24 72 S 0 1 5 0 2 I 0 588 3 0 2 S 1 2 M 87 261 5 0 2 I 0 1942 3 0 2 M 36 108 S 0 1 5 0 2 I 0 3187 3 0 2 S 1 2 M 15 45 S 0 2 5 0 2 I 0 1904 3 0 2 S 1 1 M 60 180 5 0 2 I 0 620 3 0 2 M 61 183 5 0 2 I 0 4257 3 0 2 M 32 96 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-15 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local gene 22987 44683 1582 - . gene_id 1 ; sequence SPP00000074_2.0 ; gene_orientation + gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local cds 44612 44683 . - . gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local exon 44612 44683 . - . insertions 0 ; deletions 0 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice5 44610 44611 . - . intron_id 1 ; splice_site "GT" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local intron 44281 44611 . - . intron_id 1 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice3 44281 44282 . - . intron_id 0 ; splice_site "AG" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local cds 44172 44280 . - . gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local exon 44172 44280 . - . insertions 0 ; deletions 0 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice5 44170 44171 . - . intron_id 2 ; splice_site "GT" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local intron 36465 44171 . - . intron_id 2 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice3 36465 36466 . - . intron_id 1 ; splice_site "AG" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local cds 36390 36464 . - . gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local exon 36390 36464 . - . insertions 0 ; deletions 0 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice5 36388 36389 . - . intron_id 3 ; splice_site "GT" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local intron 35798 36389 . - . intron_id 3 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice3 35798 35799 . - . intron_id 2 ; splice_site "AG" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local cds 35535 35797 . - . gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local exon 35535 35797 . - . insertions 0 ; deletions 0 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice5 35533 35534 . - . intron_id 4 ; splice_site "GT" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local intron 33589 35534 . - . intron_id 4 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice3 33589 33590 . - . intron_id 3 ; splice_site "AG" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local cds 33480 33588 . - . gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local exon 33480 33588 . - . insertions 0 ; deletions 0 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice5 33478 33479 . - . intron_id 5 ; splice_site "GT" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local intron 30289 33479 . - . intron_id 5 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice3 30289 30290 . - . intron_id 4 ; splice_site "AG" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local cds 30240 30288 . - . gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local exon 30240 30288 . - . insertions 0 ; deletions 0 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice5 30238 30239 . - . intron_id 6 ; splice_site "GT" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local intron 28332 30239 . - . intron_id 6 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice3 28332 28333 . - . intron_id 5 ; splice_site "AG" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local cds 28151 28331 . - . gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local exon 28151 28331 . - . insertions 0 ; deletions 0 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice5 28149 28150 . - . intron_id 7 ; splice_site "GT" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local intron 27527 28150 . - . intron_id 7 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice3 27527 27528 . - . intron_id 6 ; splice_site "AG" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local cds 27344 27526 . - . gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local exon 27344 27526 . - . insertions 0 ; deletions 0 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice5 27342 27343 . - . intron_id 8 ; splice_site "GT" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local intron 23083 27343 . - . intron_id 8 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local splice3 23083 23084 . - . intron_id 7 ; splice_site "AG" gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local cds 22987 23082 . - . gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local exon 22987 23082 . - . insertions 0 ; deletions 0 gi|397463253|gb|JH732248.1|:subseq(0,80000) exonerate:protein2genome:local similarity 22987 44683 1582 - . alignment_id 1 ; Query SPP00000074_2.0 ; Align 44684 19 72 ; Align 44281 43 108 ; Align 36463 80 72 ; Align 35796 105 261 ; Align 33589 192 108 ; Align 30287 229 45 ; Align 28331 245 180 ; Align 27527 305 183 ; Align 23083 366 96 # --- END OF GFF DUMP --- # -- completed exonerate analysis