TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000074_2.0 # Protein # Selenoprotein I (SelI) # Human
(Homo sapiens)
(397 letters)
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g
enome.fa
14,644 sequences; 2,174,243,242 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|397463253|gb|JH732248.1| Alligator mississippiensis unplaced ... 147 6e-34
gi|397457187|gb|JH738313.1| Alligator mississippiensis unplaced ... 109 2e-22
gi|397461419|gb|JH734082.1| Alligator mississippiensis unplaced ... 65 5e-09
gi|397463844|gb|JH731657.1| Alligator mississippiensis unplaced ... 64 1e-08
gi|397457775|gb|JH737726.1| Alligator mississippiensis unplaced ... 37 1.2
gi|397460674|gb|JH734827.1| Alligator mississippiensis unplaced ... 37 2.1
gi|397460935|gb|JH734566.1| Alligator mississippiensis unplaced ... 36 3.5
gi|397461083|gb|JH734418.1| Alligator mississippiensis unplaced ... 36 3.5
gi|397456498|gb|JH739002.1| Alligator mississippiensis unplaced ... 35 4.6
gi|397460765|gb|JH734736.1| Alligator mississippiensis unplaced ... 35 4.6
gi|397457091|gb|JH738409.1| Alligator mississippiensis unplaced ... 35 6.0
gi|397455923|gb|JH739577.1| Alligator mississippiensis unplaced ... 35 7.9
gi|397460709|gb|JH734792.1| Alligator mississippiensis unplaced ... 35 7.9
>gi|397463253|gb|JH732248.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-1979, whole genome shotgun sequence
Length = 214378
Score = 147 bits (372), Expect = 6e-34
Identities = 82/174 (47%), Positives = 101/174 (58%), Gaps = 13/174 (7%)
Frame = -3
Query: 87 DWVWIVVGILNFVAYTL-DGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIFG 145
+W+ + G+ ++ L DGVDGKQARRTNSSTPLGELFDHGLDSW+C+YFVVTVYS FG
Sbjct: 35852 EWLAVPGGVQMCISACLPDGVDGKQARRTNSSTPLGELFDHGLDSWACMYFVVTVYSTFG 35673
Query: 146 RGSTGXXXXXXXXXXXXXXXXXILSHWEKYNTGILFLPWGYDISQVTISFVYIV------ 199
RGSTG ILSHWEKYNTGILFLPWGYDISQ+ F ++
Sbjct: 35672 RGSTGVSVFVLYLLLWVVLFSFILSHWEKYNTGILFLPWGYDISQMVSMFFLLLNLK*SF 35493
Query: 200 ----TAVVGVEAW--YEPFLFNFLYRDLFTAMIIGCALCVTLPMSLLNFFRSYK 247
+ G+ W Y L L+ + I C +L + LL F ++
Sbjct: 35492 RIQ*ASTGGLPKWCVYGYMLLWHLFSLARIQLFIKCPSPASLDLGLLCLFLDFR 35331
Score = 91.3 bits (225), Expect = 7e-17
Identities = 42/66 (63%), Positives = 53/66 (80%)
Frame = -1
Query: 244 RSYKNNTLKLNSVYEAMVPLFSPCLLFILSTAWILWSPSDILELHPRVFYFMVGTAFANS 303
R+YK+NTLK +S+YE M+P SP LLF L T WI SPSDILE+HPR+FYFMVGTAFAN
Sbjct: 28333 RAYKSNTLKHHSLYEIMLPFLSPVLLFALCTTWIFVSPSDILEVHPRLFYFMVGTAFANI 28154
Query: 304 TCQLIV 309
+ + ++
Sbjct: 28153 SVRALL 28136
Score = 73.9 bits (180), Expect = 1e-11
Identities = 32/36 (88%), Positives = 34/36 (94%)
Frame = -2
Query: 43 VFPTWLAPNLITFSGFLLVVFNFLLMAYFDPDFYAS 78
+FPTWLAPNLITF GFLL+VFNF LMAYFDPDFYAS
Sbjct: 44280 IFPTWLAPNLITFCGFLLLVFNFFLMAYFDPDFYAS 44173
Score = 69.7 bits (169), Expect = 2e-10
Identities = 33/42 (78%), Positives = 36/42 (85%)
Frame = -2
Query: 192 TISFVYIVTAVVGVEAWYEPFLFNFLYRDLFTAMIIGCALCV 233
TIS VYIVTA+VGVEAW+ PFLFNFLYRDLFT MI+G L V
Sbjct: 33588 TISIVYIVTAIVGVEAWHAPFLFNFLYRDLFTTMILGKKLHV 33463
Score = 53.5 bits (127), Expect = 2e-05
Identities = 22/25 (88%), Positives = 25/25 (100%)
Frame = -1
Query: 19 KYSAVDTNPLSLYVMHPFWNTIVKV 43
+YSAVD+NPLSLYVMHPFWNT+VKV
Sbjct: 44683 QYSAVDSNPLSLYVMHPFWNTVVKV 44609
Score = 47.8 bits (112), Expect = 9e-04
Identities = 26/64 (40%), Positives = 29/64 (45%)
Frame = -1
Query: 305 CQLIVCQMSSTRCPTLNWXXXXXXXXXXXXXXXXASYVESIXXXXXXXXXXXXHIHYGVR 364
CQLIVCQMS+TRC LNW A E+I HIHYGV
Sbjct: 27526 CQLIVCQMSNTRCQPLNWMLLPLAVVVFVVLYGLAPNNETILLYTLTAVITVAHIHYGVC 27347
Query: 365 VVKQ 368
VV +
Sbjct: 27346 VVSE 27335
Score = 45.4 bits (106), Expect = 0.004
Identities = 18/27 (66%), Positives = 24/27 (88%)
Frame = -2
Query: 77 ASAPGHKHVPDWVWIVVGILNFVAYTL 103
+SAP H HVP+ +WI+VG+LNF+AYTL
Sbjct: 36471 SSAPDHVHVPNRIWIMVGLLNFMAYTL 36391
Score = 39.7 bits (91), Expect = 0.24
Identities = 19/32 (59%), Positives = 24/32 (75%)
Frame = -2
Query: 366 VKQLSSHFQIYPFSLRKPNSDXLGMEEKNIGL 397
V QLS HF I PFSL+K ++D L +EE+ IGL
Sbjct: 23082 VNQLSKHFNIRPFSLKKRSTD*LEVEEQKIGL 22987
>gi|397457187|gb|JH738313.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-11980, whole genome shotgun sequence
Length = 660312
Score = 109 bits (273), Expect = 2e-22
Identities = 53/107 (49%), Positives = 70/107 (65%), Gaps = 3/107 (2%)
Frame = -3
Query: 88 WVWIVVGILNFVAYTL-DGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIFGR 146
W+++V+ ++ F+ D +DGK ARRT+ STPLGELFDHGLDSW+ F + +S+ R
Sbjct: 617110 WLFLVMPLIFFLFSPFPDSIDGKHARRTHCSTPLGELFDHGLDSWATSIFTLNFFSLCSR 616931
Query: 147 GS--TGXXXXXXXXXXXXXXXXXILSHWEKYNTGILFLPWGYDISQV 191
G+ TG ++SHWEKYNTG+LFLPWGYDISQV
Sbjct: 616930 GNEQTGFLLHKMFLSLSIALLNFMVSHWEKYNTGVLFLPWGYDISQV 616790
Score = 65.5 bits (158), Expect = 4e-09
Identities = 33/76 (43%), Positives = 46/76 (60%)
Frame = -2
Query: 227 IGCALCVTLPMSLLNFFRSYKNNTLKLNSVYEAMVPLFSPCLLFILSTAWILWSPSDILE 286
+G C +L +L FR+Y TLK +S Y+ VPL SP LLF L + W+ SP DIL
Sbjct: 581423 LGNVFC-SLKQIVLFIFRAYLKKTLKRDSFYDGCVPLVSPILLFTLISIWVTLSPCDILA 581247
Query: 287 LHPRVFYFMVGTAFAN 302
R+F++M+G F+N
Sbjct: 581246 KQTRMFFWMLGVTFSN 581199
Score = 51.6 bits (122), Expect = 6e-05
Identities = 22/64 (34%), Positives = 40/64 (62%)
Frame = -3
Query: 15 FDKYKYSAVDTNPLSLYVMHPFWNTIVKVFPTWLAPNLITFSGFLLVVFNFLLMAYFDPD 74
F YK L ++ P + +++ P W+APNL+TFSGF++++ N+ L++++D D
Sbjct: 623818 FKDYKMVGYKNGFLMPNLILPLF*IFLQIVPQWVAPNLLTFSGFVVLLINYFLLSFYDWD 623639
Query: 75 FYAS 78
+ AS
Sbjct: 623638 YTAS 623627
Score = 42.7 bits (99), Expect = 0.029
Identities = 18/25 (72%), Positives = 22/25 (88%)
Frame = -1
Query: 19 KYSAVDTNPLSLYVMHPFWNTIVKV 43
+YSAVDTNPLS++VM P W+ IVKV
Sbjct: 627327 QYSAVDTNPLSVHVMQPMWDQIVKV 627253
Score = 37.0 bits (84), Expect = 1.6
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = -1
Query: 196 VYIVTAVVGVEAWYEPFLFNFLYRDLFTAMIIG 228
+Y++TA GVE W+ P LF + D+ +IIG
Sbjct: 606063 LYLLTAATGVEIWHRPILFGYYIVDILILLIIG 605965
>gi|397461419|gb|JH734082.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-4968, whole genome shotgun sequence
Length = 1845914
Score = 65.1 bits (157), Expect = 5e-09
Identities = 30/47 (63%), Positives = 34/47 (72%)
Frame = +3
Query: 86 PDWVWIVVGILNFVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWS 132
P W +I F+ +LD +DGKQARRTNSSTPLGELFDHG DS S
Sbjct: 1389954 PPWTYIACACGLFIYQSLDAIDGKQARRTNSSTPLGELFDHGCDSLS 1390094
Score = 45.1 bits (105), Expect = 0.006
Identities = 22/67 (32%), Positives = 37/67 (55%)
Frame = +1
Query: 7 VSPEQLAGFDKYKYSAVDTNPLSLYVMHPFWNTIVKVFPTWLAPNLITFSGFLLVVFNFL 66
+S QL +++KY + + L +M +W +V P W+APNLIT G L+ + L
Sbjct: 1377187 LSKHQLKRLEEHKYQSSGRSLLEP-LMQGYWEWLVGRVPAWIAPNLITIVGLLINICTTL 1377363
Query: 67 LMAYFDP 73
++ Y+ P
Sbjct: 1377364 ILVYYCP 1377384
>gi|397463844|gb|JH731657.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-1080, whole genome shotgun sequence
Length = 304818
Score = 63.9 bits (154), Expect = 1e-08
Identities = 29/47 (61%), Positives = 37/47 (78%)
Frame = -1
Query: 86 PDWVWIVVGILNFVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWS 132
P WV+++ + F+ +LD +DGKQARRTNSS+PLGELFDHG DS S
Sbjct: 117234 PFWVFLLCAVGLFLYQSLDAIDGKQARRTNSSSPLGELFDHGCDSVS 117094
Score = 41.2 bits (95), Expect = 0.084
Identities = 26/68 (38%), Positives = 35/68 (51%), Gaps = 4/68 (5%)
Frame = -3
Query: 5 EYVSPEQLAGFDKYKYSAVDTN----PLSLYVMHPFWNTIVKVFPTWLAPNLITFSGFLL 60
E +S QL +++ YSA + PL +Y W +V+ P WLAPN IT G LL
Sbjct: 131494 EPLSLAQLRRLEQHSYSAAGRSLLEPPLQVY-----WGWLVQRTPAWLAPNAITMGGLLL 131330
Query: 61 VVFNFLLM 68
V LL+
Sbjct: 131329 NVLPTLLL 131306
>gi|397457775|gb|JH737726.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-10983, whole genome shotgun sequence
Length = 879121
Score = 37.4 bits (85), Expect = 1.2
Identities = 24/73 (32%), Positives = 32/73 (43%), Gaps = 2/73 (2%)
Frame = +1
Query: 218 YRD--LFTAMIIGCALCVTLPMSLLNFFRSYKNNTLKLNSVYEAMVPLFSPCLLFILSTA 275
YRD LF ++ C L L M L F NN K + E + CLL ST
Sbjct: 809677 YRDFFLFHVSLVRCFLATILTMLLDCIFLFQFNNLSKGCTTSE*LGVACQVCLLDRFSTM 809856
Query: 276 WILWSPSDILELH 288
W +W+P ++ H
Sbjct: 809857 WAVWAPQSLMSTH 809895
>gi|397460674|gb|JH734827.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-6199, whole genome shotgun sequence
Length = 148872
Score = 36.6 bits (83), Expect = 2.1
Identities = 25/68 (36%), Positives = 31/68 (45%)
Frame = +1
Query: 197 YIVTAVVGVEAWYEPFLFNFLYRDLFTAMIIGCALCVTLPMSLLNFFRSYKNNTLKLNSV 256
YI+ GV P +F+ L LF A + C L + L + L NF SY NTL S
Sbjct: 13153 YILQGTDGVSV--PPLIFDTLVNFLFIAFCVPC*LNLKLCLGLFNFLSSYLCNTLIFFSS 13326
Query: 257 YEAMVPLF 264
PLF
Sbjct: 13327 TMTKFPLF 13350
>gi|397460935|gb|JH734566.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-5761, whole genome shotgun sequence
Length = 1232591
Score = 35.8 bits (81), Expect = 3.5
Identities = 27/114 (23%), Positives = 49/114 (42%), Gaps = 10/114 (8%)
Frame = -3
Query: 210 EPFLFNFLYRDLFTAMIIGCALCVTLPMSLLNFFRSYKN----------NTLKLNSVYEA 259
+P + NF+Y ++ +C P N++RS N N N
Sbjct: 432876 KPPIINFIYEKIY--------ICYYFPRMKSNYWRSALNLSPSHCYSRENKSSCNPFTLC 432721
Query: 260 MVPLFSPCLLFILSTAWILWSPSDILELHPRVFYFMVGTAFANSTCQLIVCQMS 313
++P SPC F LS + +PS+ +EL ++F+F V + S ++ ++
Sbjct: 432720 LLPTSSPCSPFPLSLL-LDSAPSEHMELRSKIFFF*VDIKHSCSNLHIVAVDLA 432562
>gi|397461083|gb|JH734418.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-5522, whole genome shotgun sequence
Length = 842648
Score = 35.8 bits (81), Expect = 3.5
Identities = 20/62 (32%), Positives = 33/62 (53%)
Frame = -1
Query: 195 FVYIVTAVVGVEAWYEPFLFNFLYRDLFTAMIIGCALCVTLPMSLLNFFRSYKNNTLKLN 254
+ ++V V ++ WY NFLY+ F A LCV++P+ +L RSY ++ +
Sbjct: 275648 YFFLVHQEVFIQVWY--LSLNFLYKSTFAAF*TMKLLCVSIPIQIL---RSYSDSHFHFH 275484
Query: 255 SV 256
SV
Sbjct: 275483 SV 275478
>gi|397456498|gb|JH739002.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-13157, whole genome shotgun sequence
Length = 646375
Score = 35.4 bits (80), Expect = 4.6
Identities = 24/68 (35%), Positives = 33/68 (48%), Gaps = 5/68 (7%)
Frame = -1
Query: 214 FNFLYRDLFTAMIIGCALCVTLPMSLLNFFRSYKNNTLKLNSVYEAMVPLF-----SPCL 268
F+FLY LF I P SLL+ F Y + + S+ +VP++ SPC
Sbjct: 221530 FSFLYHTLFLHRI---------PTSLLHLFSPY----MHIVSILHTLVPVYFRPIPSPCF 221390
Query: 269 LFILSTAW 276
FI+S AW
Sbjct: 221389 HFIVSYAW 221366
>gi|397460765|gb|JH734736.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-6043, whole genome shotgun sequence
Length = 204872
Score = 35.4 bits (80), Expect = 4.6
Identities = 23/71 (32%), Positives = 33/71 (46%)
Frame = -1
Query: 211 PFLFNFLYRDLFTAMIIGCALCVTLPMSLLNFFRSYKNNTLKLNSVYEAMVPLFSPCLLF 270
PF+ N L + L + + C L + L L +FF +Y +NTL L + + PL LL
Sbjct: 78401 PFVANILIKTLLVTLHVPCQLQLQLYFGLPDFFPAYLSNTLILFPSFLSKCPLLVSFLLV 78222
Query: 271 ILSTAWILWSP 281
T I P
Sbjct: 78221 FKFTEEISAKP 78189
>gi|397457091|gb|JH738409.1| Alligator mississippiensis unplaced
genomic scaffold scaffold-12134, whole genome shotgun
sequence
Length = 115974
Score = 35.0 bits (79), Expect = 6.0
Identities = 32/109 (29%), Positives = 44/109 (40%), Gaps = 13/109 (11%)
Frame = +2
Query: 209 YEPFLFNFLYRDLFTAMIIGCALCVTLPMSLLNFFRSYKNNTLKLNSVYEAMVPLFSPCL 268
+ P N L L + I C L + L + L +F + +NTL L Y + PLF L
Sbjct: 341 HPPLATNILIETLLVTLRIPCWLQLQLLLGLPDFIPACLSNTLILLHSYFSKFPLFISFL 520
Query: 269 LFILSTAWILWSPS-------------DILELHPRVFYFMVGTAFANST 304
I T L PS D L LH + +F V A +S+
Sbjct: 521 FVI*FTEQFLAKPSWSSAIPAILPVRWDSLFLHSQQVFFEVQPALLDSS 667
>gi|397455923|gb|JH739577.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-14123, whole genome shotgun sequence
Length = 337447
Score = 34.7 bits (78), Expect = 7.9
Identities = 27/87 (31%), Positives = 37/87 (42%), Gaps = 19/87 (21%)
Frame = -3
Query: 228 GCALCVTLPMSLLNFFRSYKNNTLKLNSVYEAMVPLFSP--CLLFILSTAWI-------- 277
G +CV + S+LNF N+ K + + P FSP CL ILS +I
Sbjct: 143177 GQFVCVCIICSILNFLALEDNSQSKFKTPWAPCSPSFSPRSCLCIILS*PFILGTFCKSL 142998
Query: 278 ---------LWSPSDILELHPRVFYFM 295
L+ PS +L LHP F+
Sbjct: 142997 LHTQELSLFLFFPSVVLSLHPHTGLFV 142917
>gi|397460709|gb|JH734792.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-6140, whole genome shotgun sequence
Length = 868369
Score = 34.7 bits (78), Expect = 7.9
Identities = 20/54 (37%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Frame = +1
Query: 231 LCVTLPMSLLNFFRSYKNNTLKLNSVYEAMVPLFSPCLLFILSTAWI-LWSPSD 283
L T+P++ FR YK LK+ + EA P FS LF++ W+ +SP D
Sbjct: 570628 LTSTIPLAY-GLFRQYKKE-LKVRGLEEARYPRFSKACLFVMGVKWVSAFSPGD 570783
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis
/genome.fa
Posted date: Oct 18, 2013 5:53 PM
Number of letters in database: 2,174,243,242
Number of sequences in database: 14,644
Lambda K H
0.327 0.141 0.463
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,161,424,374
Number of Sequences: 14644
Number of extensions: 18876921
Number of successful extensions: 131082
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 948
Number of HSP's successfully gapped in prelim test: 839
Number of HSP's that attempted gapping in prelim test: 119291
Number of HSP's gapped (non-prelim): 34291
length of query: 397
length of database: 724,747,747
effective HSP length: 132
effective length of query: 265
effective length of database: 722,814,739
effective search space: 191545905835
effective search space used: 191545905835
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 77 (34.3 bits)