TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000071_2.0 # Protein # Selenoprotein H (SelH) # Human (Homo sapiens) (122 letters) Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g enome.fa 14,644 sequences; 2,174,243,242 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|397463590|gb|JH731911.1| Alligator mississippiensis unplaced ... 63 3e-09 gi|397460563|gb|JH734938.1| Alligator mississippiensis unplaced ... 34 1.3 gi|397462872|gb|JH732629.1| Alligator mississippiensis unplaced ... 32 3.7 gi|397460904|gb|JH734597.1| Alligator mississippiensis unplaced ... 32 4.8 gi|397458122|gb|JH737379.1| Alligator mississippiensis unplaced ... 32 6.3 >gi|397463590|gb|JH731911.1| Alligator mississippiensis unplaced genomic scaffold scaffold-1455, whole genome shotgun sequence Length = 452107 Score = 62.8 bits (151), Expect = 3e-09 Identities = 30/46 (65%), Positives = 37/46 (80%) Frame = +1 Query: 45 RVYGRNAAALSQALRLEAPELPVKVNPTKPRRGSFEVTLLRPDGSS 90 RVYGRNA+ALS+ALR L V++NP KPRR SFEV+L++ DGSS Sbjct: 227293 RVYGRNASALSEALRHAVDNLAVEINPEKPRRNSFEVSLVKEDGSS 227430 Score = 55.5 bits (132), Expect = 4e-07 Identities = 24/31 (77%), Positives = 28/31 (90%) Frame = +3 Query: 92 ELWTGIKKGPPRKLKFPEPQEVVEELKKYLS 122 ELW+GIKKGPPRKLKFPEP++VVE LK L+ Sbjct: 227751 ELWSGIKKGPPRKLKFPEPEKVVEALKSSLA 227843 >gi|397460563|gb|JH734938.1| Alligator mississippiensis unplaced genomic scaffold scaffold-6379, whole genome shotgun sequence Length = 348239 Score = 33.9 bits (76), Expect = 1.3 Identities = 23/71 (32%), Positives = 29/71 (40%) Frame = +3 Query: 41 CTSXRVYGRNAAALSQALRLEAPELPVKVNPTKPRRGSFEVTLLRPDGSSAELWTGIKKG 100 C+ R G +A + RL A LP T E+ LL DG A W G +G Sbjct: 65757 CSELRALGSSAFSSGATCRLYASCLPGSSRAT-------ELCLLLQDGQWAHSWHGAPEG 65915 Query: 101 PPRKLKFPEPQ 111 K + P PQ Sbjct: 65916 KTAKWRSPSPQ 65948 >gi|397462872|gb|JH732629.1| Alligator mississippiensis unplaced genomic scaffold scaffold-2560, whole genome shotgun sequence Length = 573371 Score = 32.3 bits (72), Expect = 3.7 Identities = 33/96 (34%), Positives = 41/96 (42%), Gaps = 16/96 (16%) Frame = +1 Query: 28 GEGMEEATVV----IEHCTSXRVYGRNAAALSQALRLEAPELPVKVNPTKPRRGSFEVTL 83 GEG ++ V HC S RV GR AA L AL L P P +PT P RG++ +T Sbjct: 64669 GEGEQDTWVSSPSPAPHCPS-RVGGREAA-LDLALCLSFPFAPTSSHPTFP*RGNWALTR 64842 Query: 84 LRPDGSS------------AELWTGIKKGPPRKLKF 107 P S E+W K + LKF Sbjct: 64843 PLPGPHSRSRAESQDIYREGEVWREEKLMVQKPLKF 64950 >gi|397460904|gb|JH734597.1| Alligator mississippiensis unplaced genomic scaffold scaffold-5811, whole genome shotgun sequence Length = 304871 Score = 32.0 bits (71), Expect = 4.8 Identities = 23/52 (44%), Positives = 28/52 (53%), Gaps = 5/52 (9%) Frame = -2 Query: 60 LEAPELPVKVNPTKPRRGSFEVTLLRPDGSSAELWTGI-----KKGPPRKLK 106 L++P+ PV +N TKPR + TL RP G A LW G G RKLK Sbjct: 107458 LQSPKCPVGMN-TKPRGPAQIPTLHRPCGDHA*LWKGALLAARPGGGSRKLK 107306 >gi|397458122|gb|JH737379.1| Alligator mississippiensis unplaced genomic scaffold scaffold-10406, whole genome shotgun sequence Length = 1825572 Score = 31.6 bits (70), Expect = 6.3 Identities = 19/40 (47%), Positives = 21/40 (52%) Frame = +3 Query: 74 PRRGSFEVTLLRPDGSSAELWTGIKKGPPRKLKFPEPQEV 113 PRRGS LLRP G +A PPR L +P P EV Sbjct: 988302 PRRGSEAGLLLRPAGGAARTRA---PAPPRPLPWPLPPEV 988412 Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis /genome.fa Posted date: Oct 18, 2013 5:53 PM Number of letters in database: 2,174,243,242 Number of sequences in database: 14,644 Lambda K H 0.312 0.132 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 266,998,775 Number of Sequences: 14644 Number of extensions: 3730020 Number of successful extensions: 11131 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 454 Number of HSP's successfully gapped in prelim test: 230 Number of HSP's that attempted gapping in prelim test: 10259 Number of HSP's gapped (non-prelim): 1973 length of query: 122 length of database: 724,747,747 effective HSP length: 97 effective length of query: 25 effective length of database: 723,327,279 effective search space: 18083181975 effective search space used: 18083181975 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 68 (30.8 bits)