TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000067_2.0 # Protein # 15 kDa selenoprotein (Sel15) #
Human (Homo sapiens)
(162 letters)
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g
enome.fa
14,644 sequences; 2,174,243,242 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|397456477|gb|JH739023.1| Alligator mississippiensis unplaced ... 87 2e-16
>gi|397456477|gb|JH739023.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-13193, whole genome shotgun sequence
Length = 2201043
Score = 87.4 bits (215), Expect = 2e-16
Identities = 43/52 (82%), Positives = 46/52 (88%)
Frame = +3
Query: 111 KLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLERI 162
K+ ++YVRGSDPVLKLLDD GNIAEELSILKWNTDSVEEFLSEKLERI
Sbjct: 665694 KVIPFFSLQYVRGSDPVLKLLDDTGNIAEELSILKWNTDSVEEFLSEKLERI 665849
Score = 71.2 bits (173), Expect = 1e-11
Identities = 34/60 (56%), Positives = 40/60 (66%)
Frame = +3
Query: 26 VSAFGAEFSSEACRELGFXXXXXXXXXXXXGQFNLLQLDPDCRGCCQEEAQFETKKLYAG 85
+S +G + SSEACRELGF GQF+L QLDP CR CCQEEAQFET+K+ G
Sbjct: 615420 LSVYGTKLSSEACRELGFSSNLLCSSCSLLGQFSLNQLDPFCRQCCQEEAQFETRKVCFG 615599
Score = 47.4 bits (111), Expect = 2e-04
Identities = 22/27 (81%), Positives = 24/27 (88%)
Frame = +2
Query: 76 QFETKKLYAGAILEVCGXKLGRFPQVQ 102
QF T +LYAGA+LEVCG KLGRFPQVQ
Sbjct: 643025 QFFTLQLYAGAVLEVCG*KLGRFPQVQ 643105
Score = 43.1 bits (100), Expect = 0.004
Identities = 25/48 (52%), Positives = 28/48 (58%)
Frame = +3
Query: 98 FPQVQAFVRSDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILK 145
FP AFVRSDKPKLFRGLQIK P + N + EE S+ K
Sbjct: 663183 FPLTLAFVRSDKPKLFRGLQIKVSNDFSPFIL----NSSY*EEFSVHK 663314
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis
/genome.fa
Posted date: Oct 18, 2013 5:53 PM
Number of letters in database: 2,174,243,242
Number of sequences in database: 14,644
Lambda K H
0.322 0.140 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 388,450,190
Number of Sequences: 14644
Number of extensions: 4530048
Number of successful extensions: 14758
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 240
Number of HSP's successfully gapped in prelim test: 77
Number of HSP's that attempted gapping in prelim test: 14275
Number of HSP's gapped (non-prelim): 1102
length of query: 162
length of database: 724,747,747
effective HSP length: 119
effective length of query: 43
effective length of database: 723,005,111
effective search space: 31089219773
effective search space used: 31089219773
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 70 (31.6 bits)