TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000067_2.0 # Protein # 15 kDa selenoprotein (Sel15) # Human (Homo sapiens) (162 letters) Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g enome.fa 14,644 sequences; 2,174,243,242 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|397456477|gb|JH739023.1| Alligator mississippiensis unplaced ... 87 2e-16 >gi|397456477|gb|JH739023.1| Alligator mississippiensis unplaced genomic scaffold scaffold-13193, whole genome shotgun sequence Length = 2201043 Score = 87.4 bits (215), Expect = 2e-16 Identities = 43/52 (82%), Positives = 46/52 (88%) Frame = +3 Query: 111 KLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLERI 162 K+ ++YVRGSDPVLKLLDD GNIAEELSILKWNTDSVEEFLSEKLERI Sbjct: 665694 KVIPFFSLQYVRGSDPVLKLLDDTGNIAEELSILKWNTDSVEEFLSEKLERI 665849 Score = 71.2 bits (173), Expect = 1e-11 Identities = 34/60 (56%), Positives = 40/60 (66%) Frame = +3 Query: 26 VSAFGAEFSSEACRELGFXXXXXXXXXXXXGQFNLLQLDPDCRGCCQEEAQFETKKLYAG 85 +S +G + SSEACRELGF GQF+L QLDP CR CCQEEAQFET+K+ G Sbjct: 615420 LSVYGTKLSSEACRELGFSSNLLCSSCSLLGQFSLNQLDPFCRQCCQEEAQFETRKVCFG 615599 Score = 47.4 bits (111), Expect = 2e-04 Identities = 22/27 (81%), Positives = 24/27 (88%) Frame = +2 Query: 76 QFETKKLYAGAILEVCGXKLGRFPQVQ 102 QF T +LYAGA+LEVCG KLGRFPQVQ Sbjct: 643025 QFFTLQLYAGAVLEVCG*KLGRFPQVQ 643105 Score = 43.1 bits (100), Expect = 0.004 Identities = 25/48 (52%), Positives = 28/48 (58%) Frame = +3 Query: 98 FPQVQAFVRSDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILK 145 FP AFVRSDKPKLFRGLQIK P + N + EE S+ K Sbjct: 663183 FPLTLAFVRSDKPKLFRGLQIKVSNDFSPFIL----NSSY*EEFSVHK 663314 Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis /genome.fa Posted date: Oct 18, 2013 5:53 PM Number of letters in database: 2,174,243,242 Number of sequences in database: 14,644 Lambda K H 0.322 0.140 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 388,450,190 Number of Sequences: 14644 Number of extensions: 4530048 Number of successful extensions: 14758 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 240 Number of HSP's successfully gapped in prelim test: 77 Number of HSP's that attempted gapping in prelim test: 14275 Number of HSP's gapped (non-prelim): 1102 length of query: 162 length of database: 724,747,747 effective HSP length: 119 effective length of query: 43 effective length of database: 723,005,111 effective search space: 31089219773 effective search space used: 31089219773 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 70 (31.6 bits)