C4 Alignment: ------------ Query: SPP00000064_2.0 # Protein # Selenophosphate synthetase 2 (SPS2) # Human (Homo sapiens) Target: gi|397458164|gb|JH737337.1|:subseq(347813,73300) Alligator mississippiensis unplaced genomic scaffold scaffold-10337, whole genome shotgun sequence Model: protein2genome:local Raw score: 1587 Query range: 1 -> 444 Target range: 14401 -> 53565 2 : AlaGluAlaSerAlaThrGlyAlaCysGlyGluAlaMetAlaAla{A} >>>> Targe : 17 ::!..!|||:!!!.!.!!||||||...|||..!||| !||||||{!} 5 SerArgAlaAlaGlyAlaGlyAlaAlaGlyArgAlaGluAlaAla{V}+- 14402 : AGCCGAGCGGCGGGGGCGGGGGCCGCGGGCAGGGCCGAGGCAGCA{G}gg.......... : 14450 18 : t Intron 1 >>>> {la}GluGlySerSerGlyProAlaGlyLeuThrLeuGlyArg : 30 945 bp {.!}||||||!.!||||||! ! !..!|||!:!! ! !! ! ++{al}GluGlyLysSerGlyHisLeuAsnLeuSerProHisLeu 14451 : ...............ag{TA}GAAGGCAAGTCAGGGCACCTTAACCTCAGCCCCCACCTT : 20431 31 : Ser >>>> Target Intron 2 >>>> PheSerAsnTyrArgProPheGluProG : 41 ..! 29554 bp ! !!..!||||||||||||||||||: Gln++ -+ProProGlyTyrArgProPheGluProG 20432 : CAGgt.........................cgCCTCCGGGCTACCGGCCCTTCGAGCCTG : 50018 42 : lnAlaLeuGlyLeuSerProSerTrpArgLeuThrGlyPheSerGlyMetLysGlyUnkG : 61 !!||||||||||||||||||.!!||||||||||||||||||.!!! !:!!:::||| | luAlaLeuGlyLeuSerProGlyTrpArgLeuThrGlyPheGlyGluLeuArgGly***G 50019 : AGGCTCTAGGGCTGAGCCCGGGATGGCGCCTCACCGGCTTCGGGGAGCTGCGCGGCTGAG : 50078 62 : lyCysLysValProGlnGluAlaLeuLeuLysLeuLeuAlaGlyLeuThrArgProAspV : 81 ||||||||||||||||||||.!!||||||!:!||||||||||||||| ! !! !! !! lyCysLysValProGlnGluThrLeuLeuArgLeuLeuAlaGlyLeuGlyGlyAlaArgA 50079 : GCTGCAAAGTCCCGCAGGAGACGCTGCTCAGGCTCCTGGCGGGACTCGGCGGAGCCCGCG : 50138 82 : alArgProProLeuGlyArgGlyLeuValGlyGlyGlnGluGluAlaSer{G} >>>> : 98 .! ! ! ! ! ! !..! ! !|||..!... ||| !{ } laAlaGlyGluGlyProValSerGluProGlySerSerProProAlaLeu{G}++ 50139 : CGGCCGGGGAGGGTCCCGTGTCCGAGCCCGGCTCCTCCCCCCCCGCCCTC{G}gt..... : 50192 99 : Target Intron 3 >>>> {ln}GluAlaGlyLeuProAlaGlyAlaGlyProSerP : 110 87 bp { }! !! !||| !|||!.!:!!||| !.!! -+{ly}GlyGlnAspLeuValAlaAlaSerGlyGlyGlyC 50193 : ....................tg{GC}GGCCAGGACTTGGTAGCAGCCTCCGGTGGCGGCT : 50312 111 : roThrPheProAlaLeuGlyIleGlyMetAspSerCysValIleProLeuArgHisGlyG : 130 ! !!!. !! !!:!!||||||||||||||||||||||||||||||||||||||||||| ysPheLeuSerProValGlyIleGlyMetAspSerCysValIleProLeuArgHisGlyG 50313 : GTTTCTTGTCCCCTGTAGGAATCGGGATGGACTCGTGCGTCATTCCCCTGCGGCACGGGG : 50372 131 : lyLeuSerLeuValGlnThrThrAspPhePheTyrProLeuValGluAspProTyrMetM : 150 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| lyLeuSerLeuValGlnThrThrAspPhePheTyrProLeuValGluAspProTyrMetM 50373 : GCCTCTCTCTGGTGCAGACCACGGATTTCTTCTACCCTCTCGTGGAGGATCCTTACATGA : 50432 151 : et >>>> Target Intron 4 >>>> GlyArgIleAlaCysAlaAsnValLeuSe : 160 || 202 bp ||||||||||||||||||||||||||||| et++ ++GlyArgIleAlaCysAlaAsnValLeuSe 50433 : TGgt.........................agGGTCGCATCGCTTGTGCCAACGTGTTGAG : 50664 161 : rAspLeuTyrAlaMetGlyIleThrGluCysAspAsnMetLeuMetLeuLeuSerValSe : 180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| rAspLeuTyrAlaMetGlyIleThrGluCysAspAsnMetLeuMetLeuLeuSerValSe 50665 : CGATCTCTACGCCATGGGCATCACAGAGTGCGACAACATGCTGATGCTGCTCAGTGTCAG : 50724 181 : rGlnSerMetSerGluGlu >>>> Target Intron 5 >>>> GluArgGluLys : 190 ||||!.!|||!!!!!:||| 1029 bp |||||||||||| rGlnLysMetThrAspGlu++ ++GluArgGluLys 50725 : CCAGAAGATGACTGATGAGgt.........................agGAACGTGAGAAG : 51783 191 : ValThrProLeuMetValLysGlyPheArgAspAlaAlaGluGluGlyGlyThrAlaVal : 210 :!!! !||||||!!::!!||||||||||||||||||||||||!!:|||||||||:!!||| IleMetProLeuIleIleLysGlyPheArgAspAlaAlaGluAspGlyGlyThrSerVal 51784 : ATCATGCCACTGATCATCAAAGGTTTCCGGGATGCTGCTGAGGATGGCGGCACATCGGTG : 51843 211 : ThrGlyGlyGlnThrValValAsnProTrpIleIleIleGlyGlyValAlaThrValVal : 230 ||||||||||||||||||:!!|||||||||||||||:!!||||||||||||||||||||| ThrGlyGlyGlnThrValMetAsnProTrpIleIleValGlyGlyValAlaThrValVal 51844 : ACAGGTGGTCAGACTGTGATGAACCCCTGGATCATTGTTGGGGGTGTTGCCACTGTGGTC : 51903 231 : CysGlnProAsnGluPheIle{Me} >>>> Target Intron 6 >>>> {t}Pro : 239 |||||||||||||||||||||{||} 197 bp {|}||| CysGlnProAsnGluPheIle{Me}++ ++{t}Pro 51904 : TGCCAGCCCAATGAGTTCATC{AT}gt.........................ag{G}CCA : 52127 240 : AspSerAlaValValGlyAspValLeuValLeuThrLysProLeuGlyThrGlnValAla : 259 |||!:!|||||| !||||||||||||||||||||||||||||||||||||||||||||| AspAsnAlaValProGlyAspValLeuValLeuThrLysProLeuGlyThrGlnValAla 52128 : GACAATGCAGTTCCTGGCGATGTGCTGGTTCTCACGAAGCCCCTGGGCACACAGGTGGCT : 52187 260 : ValAsnAlaHisGlnTrpLeuAspAsn >>>> Target Intron 7 >>>> ProG : 270 |||!.!||||||||||||||||||||| 80 bp |||| ValThrAlaHisGlnTrpLeuAspAsn++ ++ProG 52188 : GTCACCGCTCACCAGTGGCTGGATAATgt.........................agCCTG : 52300 271 : luArgTrpAsnLysValLysMetValValSerArgGluGluValGluLeuAlaTyrGlnG : 290 ||||||||||||||:!!|||:!!|||||||||!:!|||!!:||||||||||||||||||| luArgTrpAsnLysIleLysLeuValValSerLysGluAspValGluLeuAlaTyrGlnG 52301 : AGCGGTGGAACAAGATCAAGCTGGTGGTCTCCAAGGAGGATGTGGAGCTAGCCTACCAGG : 52360 291 : luAlaMetPheAsnMetAlaThrLeuAsnArgThr{A} >>>> Target Intron 8 : 302 |||||||||||!:!||||||! !||||||||||||{|} 279 bp luAlaMetPheSerMetAlaMetLeuAsnArgThr{A}++ 52361 : AAGCCATGTTCAGCATGGCCATGCTGAACCGAACA{G}gt.................... : 52399 303 : >>>> {la}AlaGlyLeuMetHisThrPheAsnAlaHisAlaAlaThrAspIleThrG : 319 {||}|||.!!||||||||||||||||||||||||||||||||||||||||||| ++{la}AlaSerLeuMetHisThrPheAsnAlaHisAlaAlaThrAspIleThrG 52400 : .....ag{CG}GCCAGCCTGATGCACACTTTCAACGCTCACGCAGCCACGGACATCACGG : 52726 320 : lyPheGlyIleLeuGlyHisSerGlnAsnLeuAlaLysGlnGlnArgAsnGluValSerP : 339 ||||||||||||||||||||:!!||||||||||||!:!|||||||||:!!|||||||||| lyPheGlyIleLeuGlyHisAlaGlnAsnLeuAlaArgGlnGlnArgHisGluValSerP 52727 : GCTTCGGGATCCTGGGCCATGCGCAGAACCTAGCCAGGCAGCAGCGCCATGAAGTTTCTT : 52786 340 : heValIleHisAsnLeuProIleIleAlaLysMetAlaAlaValSerLysAlaSerGly< : 359 |||||||||||||||||||||||||||||||||||||||||||||||||||||!!!||| heValIleHisAsnLeuProIleIleAlaLysMetAlaAlaValSerLysAlaCysGlyA 52787 : TTGTCATCCACAATCTGCCCATCATTGCCAAGATGGCTGCAGTCAGCAAGGCCTGCGGGA : 52846 360 : ->ArgPheGlyLeuLeuGlnGlyThrSerAlaGluThrSer{G} >>>> Target In : 372 |||! !||||||||||||||||||||| !!|||||||||{|} 459 snArgSerGlyLeuLeuGlnGlyThrSerProGluThrSer{G}++ 52847 : ACCGCTCCGGGCTGCTTCAGGGCACGTCTCCGGAGACCTCA{G}gt.............. : 52891 373 : tron 9 >>>> {ly}GlyLeuLeuIleCysLeuProArgGluGlnAlaAlaArgPheC : 387 bp {||}||||||||||||||||||||||||||||||||||||||||||| ++{ly}GlyLeuLeuIleCysLeuProArgGluGlnAlaAlaArgPheC 52892 : ...........ag{GG}GGGCTTCTGATCTGCCTACCCCGGGAGCAGGCCGCACGCTTCT : 53392 388 : ysSerGluIleLysSerSerLysTyrGlyGluGlyHisGlnAlaTrpIleValGlyIleV : 407 ||:!!|||||||||||| !!|||:!!||||||||||||||||||||||||:!!||||||| ysAlaGluIleLysSerProLysHisGlyGluGlyHisGlnAlaTrpIleIleGlyIleV 53393 : GTGCTGAGATCAAGTCCCCCAAGCATGGTGAGGGCCACCAGGCCTGGATCATCGGCATCG : 53452 408 : alGluLysGlyAsnArgThrAlaArgIleIleAspLysProArgValIleGluValLeuP : 427 ||||||||||||||!:!|||||||||||||||||||||||||||:!!|||||||||:!!| alGluLysGlyAsnGlnThrAlaArgIleIleAspLysProArgIleIleGluValMetP 53453 : TGGAAAAGGGCAACCAGACTGCACGCATCATTGACAAGCCCCGCATCATCGAGGTGATGC : 53512 428 : roArgGlyAlaThrAlaAlaValLeuAlaProAspSerSerAsnAlaSerSer : 444 |||||||||||.!!|||.!!!.!! ! !! !!|||!:!!!! !!.!:!!||| roArgGlyAlaAlaAlaThrAlaSerProThrAspAsnThrAlaGlyAlaSer 53513 : CTCGGGGGGCTGCAGCCACTGCCTCACCCACCGACAACACAGCTGGGGCCAGC : 53565 vulgar: SPP00000064_2.0 1 444 . gi|397458164|gb|JH737337.1|:subseq(347813,73300) 14401 53565 + 1587 M 15 45 S 0 1 5 0 2 I 0 5941 3 0 2 S 1 2 M 14 42 5 0 2 I 0 29550 3 0 2 M 66 198 S 0 1 5 0 2 I 0 83 3 0 2 S 1 2 M 52 156 5 0 2 I 0 198 3 0 2 M 36 108 5 0 2 I 0 1025 3 0 2 M 51 153 S 0 2 5 0 2 I 0 193 3 0 2 S 1 1 M 30 90 5 0 2 I 0 76 3 0 2 M 33 99 S 0 1 5 0 2 I 0 275 3 0 2 S 1 2 M 56 168 G 0 3 M 13 39 S 0 1 5 0 2 I 0 455 3 0 2 S 1 2 M 72 216 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-15 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local gene 14402 53565 1587 + . gene_id 1 ; sequence SPP00000064_2.0 ; gene_orientation + gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local cds 14402 14447 . + . gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local exon 14402 14447 . + . insertions 0 ; deletions 0 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice5 14448 14449 . + . intron_id 1 ; splice_site "GG" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local intron 14448 20392 . + . intron_id 1 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice3 20391 20392 . + . intron_id 0 ; splice_site "AG" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local cds 20393 20436 . + . gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local exon 20393 20436 . + . insertions 0 ; deletions 0 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice5 20437 20438 . + . intron_id 2 ; splice_site "GT" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local intron 20437 49990 . + . intron_id 2 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice3 49989 49990 . + . intron_id 1 ; splice_site "CG" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local cds 49991 50189 . + . gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local exon 49991 50189 . + . insertions 0 ; deletions 0 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice5 50190 50191 . + . intron_id 3 ; splice_site "GT" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local intron 50190 50276 . + . intron_id 3 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice3 50275 50276 . + . intron_id 2 ; splice_site "TG" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local cds 50277 50434 . + . gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local exon 50277 50434 . + . insertions 0 ; deletions 0 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice5 50435 50436 . + . intron_id 4 ; splice_site "GT" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local intron 50435 50636 . + . intron_id 4 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice3 50635 50636 . + . intron_id 3 ; splice_site "AG" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local cds 50637 50744 . + . gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local exon 50637 50744 . + . insertions 0 ; deletions 0 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice5 50745 50746 . + . intron_id 5 ; splice_site "GT" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local intron 50745 51773 . + . intron_id 5 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice3 51772 51773 . + . intron_id 4 ; splice_site "AG" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local cds 51774 51928 . + . gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local exon 51774 51928 . + . insertions 0 ; deletions 0 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice5 51929 51930 . + . intron_id 6 ; splice_site "GT" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local intron 51929 52125 . + . intron_id 6 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice3 52124 52125 . + . intron_id 5 ; splice_site "AG" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local cds 52126 52216 . + . gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local exon 52126 52216 . + . insertions 0 ; deletions 0 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice5 52217 52218 . + . intron_id 7 ; splice_site "GT" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local intron 52217 52296 . + . intron_id 7 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice3 52295 52296 . + . intron_id 6 ; splice_site "AG" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local cds 52297 52396 . + . gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local exon 52297 52396 . + . insertions 0 ; deletions 0 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice5 52397 52398 . + . intron_id 8 ; splice_site "GT" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local intron 52397 52675 . + . intron_id 8 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice3 52674 52675 . + . intron_id 7 ; splice_site "AG" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local cds 52676 52888 . + . gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local exon 52676 52888 . + . insertions 3 ; deletions 0 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice5 52889 52890 . + . intron_id 9 ; splice_site "GT" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local intron 52889 53347 . + . intron_id 9 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local splice3 53346 53347 . + . intron_id 8 ; splice_site "AG" gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local cds 53348 53565 . + . gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local exon 53348 53565 . + . insertions 0 ; deletions 0 gi|397458164|gb|JH737337.1|:subseq(347813,73300) exonerate:protein2genome:local similarity 14402 53565 1587 + . alignment_id 1 ; Query SPP00000064_2.0 ; Align 14402 2 45 ; Align 20395 18 42 ; Align 49991 32 198 ; Align 50279 99 156 ; Align 50637 151 108 ; Align 51774 187 153 ; Align 52127 239 90 ; Align 52297 269 99 ; Align 52678 303 168 ; Align 52849 359 39 ; Align 53350 373 216 # --- END OF GFF DUMP --- # -- completed exonerate analysis