TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000064_2.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Human (Homo sapiens)
(483 letters)
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g
enome.fa
14,644 sequences; 2,174,243,242 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|397458164|gb|JH737337.1| Alligator mississippiensis unplaced ... 140 1e-31
gi|397458686|gb|JH736815.1| Alligator mississippiensis unplaced ... 124 1e-26
gi|397459433|gb|JH736068.1| Alligator mississippiensis unplaced ... 39 0.55
gi|397461907|gb|JH733594.1| Alligator mississippiensis unplaced ... 37 1.6
gi|397462332|gb|JH733169.1| Alligator mississippiensis unplaced ... 36 3.6
gi|397458889|gb|JH736612.1| Alligator mississippiensis unplaced ... 36 4.7
gi|397455769|gb|JH739731.1| Alligator mississippiensis unplaced ... 35 7.9
>gi|397458164|gb|JH737337.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-10337, whole genome shotgun sequence
Length = 421118
Score = 140 bits (354), Expect = 1e-31
Identities = 81/145 (55%), Positives = 90/145 (62%), Gaps = 29/145 (20%)
Frame = +1
Query: 35 YRPFEPQALGLSPSWRLTGFSGMKGXGCKVPQEALLKLLAGL------------------ 76
YRPFEP+ALGLSP WRLTGF ++G GCKVPQE LL+LLAGL
Sbjct: 397813 YRPFEPEALGLSPGWRLTGFGELRG*GCKVPQETLLRLLAGLGGARAAGEGPVSEPGSSP 397992
Query: 77 ----TRPDVRPPLG-----RGLVGGQEEASQEAG--LPAGAGPSPTFPALGIGMDSCVIP 125
RP VR +G RG + EA+ G L A +G +GIGMDSCVIP
Sbjct: 397993 PALGERPAVRAGVGGWHRFRGGCWAEPEAAGVGGQDLVAASGGGCFLSPVGIGMDSCVIP 398172
Query: 126 LRHGGLSLVQTTDFFYPLVEDPYMM 150
LRHGGLSLVQTTDFFYPLVEDPYMM
Sbjct: 398173 LRHGGLSLVQTTDFFYPLVEDPYMM 398247
Score = 123 bits (309), Expect = 2e-26
Identities = 64/73 (87%), Positives = 68/73 (93%), Gaps = 1/73 (1%)
Frame = +3
Query: 301 TAAGLMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSFVIHNLPIIAKMAAVSKASG-R 359
+AA LMHTFNAHAATDITGFGILGH+QNLA+QQR+EVSFVIHNLPIIAKMAAVSKA G R
Sbjct: 400485 SAASLMHTFNAHAATDITGFGILGHAQNLARQQRHEVSFVIHNLPIIAKMAAVSKACGNR 400664
Query: 360 FGLLQGTSAETSG 372
GLLQGTS ETSG
Sbjct: 400665 SGLLQGTSPETSG 400703
Score = 117 bits (292), Expect = 2e-24
Identities = 58/85 (68%), Positives = 66/85 (77%)
Frame = +3
Query: 348 AKMAAVSKASGRFGLLQGTSAETSGGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIV 407
A AV + R L + GGLLICLPREQAARFC+EIKS K+GEGHQAWI+GIV
Sbjct: 401088 AGKGAVLRLKAREAFLLTWHPISVGGLLICLPREQAARFCAEIKSPKHGEGHQAWIIGIV 401267
Query: 408 EKGNRTARIIDKPRVIEVLPRGATA 432
EKGN+TARIIDKPR+IEV+PRGA A
Sbjct: 401268 EKGNQTARIIDKPRIIEVMPRGAAA 401342
Score = 65.9 bits (159), Expect = 4e-09
Identities = 30/51 (58%), Positives = 34/51 (66%)
Frame = +2
Query: 188 REKVTPLMVKGFRDXXXXXXXXXXXXXXXXNPWIIIGGVATVVCQPNEFIM 238
REK+ PL++KGFRD NPWII+GGVATVVCQPNEFIM
Sbjct: 399590 REKIMPLIIKGFRDAAEDGGTSVTGGQTVMNPWIIVGGVATVVCQPNEFIM 399742
Score = 61.6 bits (148), Expect(2) = 2e-19
Identities = 27/33 (81%), Positives = 32/33 (96%)
Frame = +3
Query: 269 PERWNKVKMVVSREEVELAYQEAMFNMATLNRT 301
PERWNK+K+VVS+E+VELAYQEAMF+MA LNRT
Sbjct: 400110 PERWNKIKLVVSKEDVELAYQEAMFSMAMLNRT 400208
Score = 58.9 bits (141), Expect(2) = 2e-19
Identities = 27/30 (90%), Positives = 28/30 (93%)
Frame = +1
Query: 239 PDSAVVGDVLVLTKPLGTQVAVNAHQWLDN 268
PD+AV GDVLVLTKPLGTQVAV AHQWLDN
Sbjct: 399940 PDNAVPGDVLVLTKPLGTQVAVTAHQWLDN 400029
Score = 49.7 bits (117), Expect = 3e-04
Identities = 22/22 (100%), Positives = 22/22 (100%)
Frame = +2
Query: 151 GRIACANVLSDLYAMGITECDN 172
GRIACANVLSDLYAMGITECDN
Sbjct: 398450 GRIACANVLSDLYAMGITECDN 398515
>gi|397458686|gb|JH736815.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-9464, whole genome shotgun sequence
Length = 1028860
Score = 124 bits (310), Expect = 1e-26
Identities = 62/72 (86%), Positives = 66/72 (91%), Gaps = 1/72 (1%)
Frame = -3
Query: 302 AAGLMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSFVIHNLPIIAKMAAVSKASGR-F 360
AAGLMHTFNAHAATDITGFGILGH+QNLAKQQRNEVSFVIHNLP++AKMAAVSKA G F
Sbjct: 30041 AAGLMHTFNAHAATDITGFGILGHAQNLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMF 29862
Query: 361 GLLQGTSAETSG 372
GL+ GT ETSG
Sbjct: 29861 GLMHGTCPETSG 29826
Score = 115 bits (289), Expect = 4e-24
Identities = 55/64 (85%), Positives = 59/64 (92%)
Frame = -3
Query: 371 SGGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGA 430
SGGLLICLPREQAARFC+EIKS KYGEGHQAWI+GIVEKGNRTARIIDKPR+IEV P+ A
Sbjct: 21416 SGGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVA 21237
Query: 431 TAAV 434
T V
Sbjct: 21236 TQNV 21225
Score = 71.2 bits (173), Expect = 1e-10
Identities = 30/35 (85%), Positives = 34/35 (97%)
Frame = -2
Query: 116 GIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMM 150
GIGMD+CVIPLRHGGLSLVQTTD+ YP+V+DPYMM
Sbjct: 46149 GIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM 46045
Score = 60.1 bits (144), Expect = 2e-07
Identities = 26/51 (50%), Positives = 33/51 (64%)
Frame = -3
Query: 188 REKVTPLMVKGFRDXXXXXXXXXXXXXXXXNPWIIIGGVATVVCQPNEFIM 238
R+KV PL+++GF+D NPWI++GGVAT VCQPNEFIM
Sbjct: 36542 RDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM 36390
Score = 58.5 bits (140), Expect = 7e-07
Identities = 25/33 (75%), Positives = 31/33 (93%)
Frame = -2
Query: 269 PERWNKVKMVVSREEVELAYQEAMFNMATLNRT 301
PE+WNK+K+VV++E+VELAYQEAM NMA LNRT
Sbjct: 31659 PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRT 31561
Score = 54.3 bits (129), Expect = 1e-05
Identities = 25/29 (86%), Positives = 26/29 (89%)
Frame = -1
Query: 239 PDSAVVGDVLVLTKPLGTQVAVNAHQWLD 267
PD+AV GDVLVLTKPLGTQVAV HQWLD
Sbjct: 35572 PDNAVPGDVLVLTKPLGTQVAVAVHQWLD 35486
Score = 49.3 bits (116), Expect = 4e-04
Identities = 23/34 (67%), Positives = 25/34 (73%)
Frame = -1
Query: 139 FFYPLVEDPYMMGRIACANVLSDLYAMGITECDN 172
FFY + GR ACANVLSDLYAMG+TECDN
Sbjct: 37606 FFY------FFQGRXACANVLSDLYAMGVTECDN 37523
Score = 47.0 bits (110), Expect = 0.002
Identities = 23/47 (48%), Positives = 30/47 (63%)
Frame = -2
Query: 30 RSFSNYRPFEPQALGLSPSWRLTGFSGMKGXGCKVPQEALLKLLAGL 76
R+ S F P++ L S+RLT F+ +KG GCKVPQ+ L KLL L
Sbjct: 48855 RTMSVRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESL 48715
>gi|397459433|gb|JH736068.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-8225, whole genome shotgun sequence
Length = 2510647
Score = 38.9 bits (89), Expect = 0.55
Identities = 25/53 (47%), Positives = 29/53 (54%), Gaps = 1/53 (1%)
Frame = +3
Query: 67 EALLKLLAGLTRPDVRPPLGRGLVGGQEEASQE-AGLPAGAGPSPTFPALGIG 118
+A+L AGL+R V GRG EE + E AG P G GPSPT P G G
Sbjct: 400512 DAILPAPAGLSRVGVVRLPGRGRPAATEEGAGECAGPPRGRGPSPTGPGGGAG 400670
>gi|397461907|gb|JH733594.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-4143, whole genome shotgun sequence
Length = 1134123
Score = 37.4 bits (85), Expect = 1.6
Identities = 26/75 (34%), Positives = 36/75 (48%), Gaps = 5/75 (6%)
Frame = -1
Query: 75 GLTR-PDVRPPLGRGLVGGQEEASQEAGLPAGAGPSPTFPALGI----GMDSCVIPLRHG 129
G TR P V PL +G G A + G G +P FP+L + G D+CV P+RH
Sbjct: 709650 GTTRAPGVCSPLSQGPRG----APLKGGEGQGGNNNPAFPSLFL*VVWGSDACVCPIRHA 709483
Query: 130 GLSLVQTTDFFYPLV 144
G Q+ +P +
Sbjct: 709482 GPVASQSLSLSHPSI 709438
>gi|397462332|gb|JH733169.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-3480, whole genome shotgun sequence
Length = 518331
Score = 36.2 bits (82), Expect = 3.6
Identities = 31/90 (34%), Positives = 37/90 (41%), Gaps = 9/90 (10%)
Frame = +2
Query: 30 RSFSNYRPFE-------PQALGLSPSWRLTGFSGMKGXGCKVPQEALLKLLAGLTRPDVR 82
RS+S RP P GL P L G S G V + + AGL++ R
Sbjct: 131555 RSWSGRRPLPAPGSGHGPAHRGLRPPAALAGISCRSGGSRGVSRHCPCR--AGLSQAPPR 131728
Query: 83 PPLGRGLVGGQEE--ASQEAGLPAGAGPSP 110
PP G GG A AG G+GPSP
Sbjct: 131729 PPPSPGCGGGARSLPAGYTAGRTPGSGPSP 131818
>gi|397458889|gb|JH736612.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-9115, whole genome shotgun sequence
Length = 231482
Score = 35.8 bits (81), Expect = 4.7
Identities = 28/78 (35%), Positives = 33/78 (42%), Gaps = 3/78 (3%)
Frame = +3
Query: 40 PQALGLSPSWRLTGFS---GMKGXGCKVPQEALLKLLAGLTRPDVRPPLGRGLVGGQEEA 96
P L + W TG M G + PQEA + T P G GL GG+EE
Sbjct: 92550 PLVLVATHRWWRTGVGVGLPMGSPGNRWPQEAAATCMETRTHPLA---FGPGLAGGKEEC 92720
Query: 97 SQEAGLPAGAGPSPTFPA 114
AGL GP P +PA
Sbjct: 92721 ---AGLAGAPGPRPNWPA 92765
>gi|397455769|gb|JH739731.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-14377, whole genome shotgun sequence
Length = 626350
Score = 35.0 bits (79), Expect = 7.9
Identities = 17/51 (33%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
Frame = -1
Query: 238 MPDSAVVGDVL--VLTKPLGTQVAVNAHQWLDNPERWNKVKMVVSREEVEL 286
+P +V+G VL + LG V ++ DN + W KVKM+ RE++++
Sbjct: 304723 VPQGSVLGPVLFNIFISDLGEGVESTLSKFTDNTKLWGKVKMLEGREQIQV 304571
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis
/genome.fa
Posted date: Oct 18, 2013 5:53 PM
Number of letters in database: 2,174,243,242
Number of sequences in database: 14,644
Lambda K H
0.320 0.136 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,098,621,944
Number of Sequences: 14644
Number of extensions: 17523554
Number of successful extensions: 93527
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 916
Number of HSP's successfully gapped in prelim test: 1324
Number of HSP's that attempted gapping in prelim test: 82510
Number of HSP's gapped (non-prelim): 30692
length of query: 483
length of database: 724,747,747
effective HSP length: 134
effective length of query: 349
effective length of database: 722,785,451
effective search space: 252252122399
effective search space used: 252252122399
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 78 (34.7 bits)