Command line: [exonerate -m p2g --showtargetgff -q ../Sel.human/SECS.fa -t gi|397463559|gb|JH731942.1|subseq.fa -E] Hostname: [ws101066] ** (process:3698): WARNING **: Exhaustively generating suboptimal alignments will be VERY SLOW C4 Alignment: ------------ Query: SPP00000065_2.0 # Protein # Selenocysteine synthase (SecS) # Human (Homo sapiens) Target: gi|397463559|gb|JH731942.1|:subseq(600000,155000) Alligator mississippiensis unplaced genomic scaffold scaffold-1514, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 2006 Query range: 0 -> 470 Target range: 115575 -> 66275 1 : MetAsnArgGluSerPheAlaAlaGlyGluArgLeuValSerProAlaTyrValArgG : 20 |||||| !!|||!:!|||!.!|||.!!||||||||||||:!! !!||||||||||||| MetAsnGlyGluAsnPheGlyAlaSerGluArgLeuValAlaAlaAlaTyrValArgG 115575 : ATGAACGGCGAGAACTTCGGTGCCAGCGAGAGGCTGGTGGCTGCGGCCTACGTGCGGC : 115518 21 : lnGlyCysGluAlaArgArgSerHisGluHisLeuIleArgLeuLeuLeuGluLys : 39 |||||...:!!!.!||||||:!!||||||! !! !:!:||| !|||||||||:!! lnGlyAlaGlnGlyArgArgAlaHisGluLeuArgLeuArgAlaLeuLeuGluGln++ 115517 : AGGGGGCGCAGGGCCGCCGCGCGCACGAGCTCCGCCTGCGGGCGCTGCTAGAGCAGgt : 115461 40 : >>>> Target Intron 1 >>>> GlyLysCysProGluAsnGlyTrpAspGluS : 49 6776 bp |||||||||||||||:!!||||||||||||| ++GlyLysCysProGluAspGlyTrpAspGluS 115460 : .........................agGGTAAGTGTCCCGAAGATGGATGGGATGAAA : 108655 50 : erThrLeuGluLeuPheLeuHisGluLeuAlaIleMetAspSerAsnAsnPheLeuGl : 68 |||||:!!|||||||||||||||||||||||||||||||||||||||||||||||||| erThrIleGluLeuPheLeuHisGluLeuAlaIleMetAspSerAsnAsnPheLeuGl 108654 : GTACCATTGAACTCTTTCTTCATGAACTTGCCATTATGGATAGCAACAATTTTCTGGG : 108598 69 : yAsnCysGlyValGlyGluArgGluGlyArgValAlaSerAlaLeuValAlaArgArg : 87 ||||||||||||||||||||||||||||||||||||||||!.!||||||||||||||| yAsnCysGlyValGlyGluArgGluGlyArgValAlaSerGlyLeuValAlaArgArg 108597 : GAATTGTGGTGTGGGTGAGAGGGAAGGAAGAGTGGCATCAGGACTTGTTGCCCGTCGG : 108541 88 : HisTyr{Ar} >>>> Target Intron 2 >>>> {g}PheIleHisGlyIleG : 96 ||||||{||} 3953 bp {|}!!.||||||||||||| HisTyr{Ar}++ ++{g}LeuIleHisGlyIleG 108540 : CATTAC{AG}gt.........................ag{G}TTGATCCATGGAATCG : 104561 97 : lyArgSerGlyAspIleSerAlaValGlnProLysAlaAlaGlySerSerLeuLeuAs : 115 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| lyArgSerGlyAspIleSerAlaValGlnProLysAlaAlaGlySerSerLeuLeuAs 104560 : GAAGGTCAGGTGATATTTCTGCTGTCCAGCCTAAAGCAGCAGGTTCTAGCCTTTTGAA : 104504 116 : nLysIleThrAsnSerLeuValLeuAspIleIleLysLeuAla{G} >>>> Targe : 130 ||||:!!||||||! !:!!||||||||||||||||||||||||{|} nLysLeuThrAsnLeuIleValLeuAspIleIleLysLeuAla{G}++ 104503 : CAAACTTACTAATTTGATAGTTCTGGACATTATAAAGCTGGCT{G}gt.......... : 104456 131 : t Intron 3 >>>> {ly}ValHisThrValAlaAsnCysPheValValProMetA : 143 94 bp {||}|||!..||||||.!!!:!||||||||||||||||||| ++{ly}ValArgThrValThrSerCysPheValValProMetA 104455 : ...............ag{GT}GTCCGGACAGTGACCAGTTGTTTTGTGGTTCCTATGG : 104326 144 : laThrGlyMetSerLeuThrLeuCysPheLeuThrLeuArgHisLysArgProLysAl : 162 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| laThrGlyMetSerLeuThrLeuCysPheLeuThrLeuArgHisLysArgProLysAl 104325 : CAACCGGCATGAGTCTAACCCTTTGTTTCTTAACACTACGGCACAAGAGACCAAAGGC : 104269 163 : aLysTyrIleIleTrpProArgIleAspGlnLysSerCysPheLysSerMetIleThr : 181 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| aLysTyrIleIleTrpProArgIleAspGlnLysSerCysPheLysSerMetIleThr 104268 : AAAATATATTATTTGGCCACGTATAGACCAGAAATCTTGCTTTAAATCTATGATAACT : 104212 182 : Ala{G} >>>> Target Intron 4 >>>> {ly}PheGluProValValIleG : 190 |||{|} 5835 bp {||}||||||||||||||||||| Ala{G}++ ++{ly}PheGluProValValIleG 104211 : GCA{G}gt.........................ag{GT}TTTGAGCCTGTGGTGATAG : 98350 191 : luAsnValLeuGluGlyAspGluLeuArgThrAspLeuLysAlaValGluAlaLysVa : 209 |||||:!!||||||..!|||||||||||||||||||||:!!!.!||||||||||||:! luAsnIleLeuGluAsnAspGluLeuArgThrAspLeuGlnGlyValGluAlaLysIl 98349 : AGAATATATTAGAAAATGATGAGCTGCGCACAGACCTCCAGGGTGTGGAAGCTAAAAT : 98293 210 : lGlnGluLeuGlyProAspCysIleLeuCysIleHisSerThrThrSerCysPheAla : 228 !|||||||||||| !!!!: !:!!||||||:!!|||||||||||||||||||||||| eGlnGluLeuGlyAlaGluAsnValLeuCysValHisSerThrThrSerCysPheAla 98292 : CCAGGAGCTTGGGGCTGAAAATGTCCTTTGTGTTCATTCTACAACTTCCTGCTTTGCC : 98236 229 : ProArgValProAsp{Ar} >>>> Target Intron 5 >>>> {g}LeuGluG : 237 |||||||||||||||{||} 6036 bp {|}||||||| ProArgValProAsp{Ar}++ ++{g}LeuGluG 98235 : CCAAGGGTACCTGAC{AG}gt.........................ag{A}CTGGAAG : 92173 238 : luLeuAlaValIleCysAlaAsnTyrAspIleProHisIleValAsnAsnAlaTyrGl : 256 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| luLeuAlaValIleCysAlaAsnTyrAspIleProHisIleValAsnAsnAlaTyrGl 92172 : AACTGGCTGTGATTTGTGCTAATTATGACATTCCCCATATTGTCAACAATGCATATGG : 92116 257 : yValGlnSerSerLysCysMetHisLeuIleGlnGln >>>> Target Intron : 269 ||||||||||||||||||||||||||||||||||||| 3161 bp yValGlnSerSerLysCysMetHisLeuIleGlnGln++ 92115 : AGTTCAGTCTTCAAAATGTATGCATCTCATCCAGCAGgt................... : 92075 270 : 6 >>>> GlyAlaArgValGlyArgIleAspAlaPheValGlnSerLeuAspLysAs : 285 ||||||||||||||||||||||||||||||:!!||||||||||||||||| ++GlyAlaArgValGlyArgIleAspAlaPheIleGlnSerLeuAspLysAs 92074 : ......agGGTGCTCGAGTAGGTAGAATAGATGCTTTCATCCAGAGCCTGGACAAAAA : 88868 286 : nPheMetValProValGlyGlyAlaIleIleAlaGlyPheAsnAspSerPheIleGln : 304 |||||||||||||||||||||||||||||||||||||||||||||||||||||||:!! nPheMetValProValGlyGlyAlaIleIleAlaGlyPheAsnAspSerPheIleLys 88867 : TTTTATGGTTCCAGTAGGTGGAGCTATCATTGCTGGCTTCAATGATTCCTTCATAAAG : 88811 305 : GluIleSerLysMetTyrPro{G} >>>> Target Intron 7 >>>> {ly}A : 313 |||||||||||||||||||||{|} 126 bp {||}| GluIleSerLysMetTyrPro{G}++ ++{ly}A 88810 : GAGATCAGTAAAATGTATCCA{G}gt.........................ag{GA}A : 88658 314 : rgAlaSerAlaSerProSerLeuAspValLeuIleThrLeuLeuSerLeuGlySerAs : 332 |||||||||||||||||||||||||||||!!!|||||||||||||||||||||:!!|| rgAlaSerAlaSerProSerLeuAspValPheIleThrLeuLeuSerLeuGlyAlaAs 88657 : GAGCATCAGCATCTCCTTCCTTAGATGTCTTTATTACTCTACTGTCACTAGGTGCAAA : 88601 333 : nGlyTyrLysLysLeuLeuLysGluArgLys >>>> Target Intron 8 >>>> : 343 |||||||::!:!!|||||||||:!!|||||| 19806 bp nGlyTyrArgGlnLeuLeuLysGlnArgLys++ 88600 : TGGCTACCGACAGCTACTAAAACAAAGAAAGgt......................... : 88566 344 : GluMetPheSerTyrLeuSerAsnGlnIleLysLysLeuSerGluAlaTyrAsnGl : 361 ||||||||||||||||||!!!!:!:!!:!:|||||||||:!!||| !:!!||||| ++GluMetPheSerTyrLeuThrSerGluLeuLysLysLeuAlaGluIleHisAsnGl 88565 : agGAAATGTTTTCGTATCTTACAAGCGAGCTGAAGAAGTTGGCAGAAATCCACAATGA : 68708 362 : uArgLeuLeuHisThrProHisAsnProIleSerLeu{A} >>>> Target Intr : 374 |||||||||| !!||||||||||||||||||||||||{|} 617 bp uArgLeuLeuAspThrProHisAsnProIleSerLeu{A}++ 68707 : GAGACTCTTGGATACACCACACAACCCTATTTCATTA{G}gt................ : 68666 375 : on 9 >>>> {la}MetThrLeuLysThrLeuAspGluHisArgAspLysAlaValT : 389 {||}|||:!!||||||!.!||||||||||||!!!|||! ||||||| ++{la}MetSerLeuLysAsnLeuAspGluHisSerAspThrAlaValT 68665 : .........ag{CC}ATGTCGCTAAAGAACCTAGATGAACACAGTGACACTGCTGTTA : 68007 390 : hrGlnLeuGlySerMetLeuPheThrArgGlnValSerGlyAla{Ar} >>>> Tar : 404 ||||||||||||||||||||||||||||||||||||||||||||{||} hrGlnLeuGlySerMetLeuPheThrArgGlnValSerGlyAla{Ar}++ 68006 : CACAACTTGGATCTATGCTTTTTACAAGACAAGTTTCTGGAGCT{AG}gt........ : 67958 405 : get Intron 10 >>>> {g}ValValProLeuGlySerMetGlnThrValSerGl : 416 1486 bp {|}|||||||||! !..!! !..!|||||||||!:!.. ++{g}ValValProArgAsnPheGlnGlnThrValAsnAs 67957 : ..................ag{G}GTGGTTCCCCGAAATTTTCAGCAAACAGTGAATAA : 66440 417 : yTyrThrPheArgGlyPheMetSerHisThrAsnAsnTyrProCysAlaTyrLeuAsn : 435 !|||||||||!:!|||||||||||||||.!!||||||||||||||||||||||||||| nTyrThrPheLysGlyPheMetSerHisAlaAsnAsnTyrProCysAlaTyrLeuAsn 66439 : CTATACATTCAAAGGCTTTATGTCTCATGCAAATAACTACCCCTGTGCTTACCTCAAT : 66383 436 : AlaAlaSerAlaIleGlyMetLysMetGlnAspValAspLeuPheIleLysArgLeuA : 455 ||||||||||||||||||||||||! !||||||||||||:!!|||:!!|||||||||: AlaAlaSerAlaIleGlyMetLysLysGlnAspValAspMetPheLeuLysArgLeuA 66382 : GCTGCTTCAGCAATTGGCATGAAAAAGCAAGATGTGGACATGTTCCTTAAAAGACTCA : 66323 456 : spArgCysLeuLysAlaValArgLysGluArgSerLysGluSerAsp : 470 !!!:!|||||||||.!! !! !!||||||! !!:!|||! !!.!||| snLysCysLeuLysThrPheGlyLysGluThrAsnLysGlyLysAsp 66322 : ATAAGTGTTTGAAGACTTTTGGAAAAGAAACAAACAAAGGAAAAGAC : 66276 vulgar: SPP00000065_2.0 0 470 . gi|397463559|gb|JH731942.1|:subseq(600000,155000) 115575 66275 - 2006 M 38 114 5 0 2 I 0 6772 3 0 2 M 51 153 S 0 2 5 0 2 I 0 3949 3 0 2 S 1 1 M 39 117 S 0 1 5 0 2 I 0 90 3 0 2 S 1 2 M 52 156 S 0 1 5 0 2 I 0 5831 3 0 2 S 1 2 M 50 150 S 0 2 5 0 2 I 0 6032 3 0 2 S 1 1 M 34 102 5 0 2 I 0 3157 3 0 2 M 43 129 S 0 1 5 0 2 I 0 122 3 0 2 S 1 2 M 30 90 5 0 2 I 0 19802 3 0 2 M 31 93 S 0 1 5 0 2 I 0 613 3 0 2 S 1 2 M 29 87 S 0 2 5 0 2 I 0 1482 3 0 2 S 1 1 M 66 198 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-25 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local gene 66276 115575 2006 - . gene_id 1 ; sequence SPP00000065_2.0 ; gene_orientation + gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local cds 115462 115575 . - . gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local exon 115462 115575 . - . insertions 0 ; deletions 0 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice5 115460 115461 . - . intron_id 1 ; splice_site "GT" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local intron 108686 115461 . - . intron_id 1 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice3 108686 108687 . - . intron_id 0 ; splice_site "AG" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local cds 108531 108685 . - . gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local exon 108531 108685 . - . insertions 0 ; deletions 0 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice5 108529 108530 . - . intron_id 2 ; splice_site "GT" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local intron 104578 108530 . - . intron_id 2 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice3 104578 104579 . - . intron_id 1 ; splice_site "AG" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local cds 104459 104577 . - . gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local exon 104459 104577 . - . insertions 0 ; deletions 0 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice5 104457 104458 . - . intron_id 3 ; splice_site "GT" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local intron 104365 104458 . - . intron_id 3 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice3 104365 104366 . - . intron_id 2 ; splice_site "AG" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local cds 104206 104364 . - . gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local exon 104206 104364 . - . insertions 0 ; deletions 0 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice5 104204 104205 . - . intron_id 4 ; splice_site "GT" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local intron 98371 104205 . - . intron_id 4 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice3 98371 98372 . - . intron_id 3 ; splice_site "AG" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local cds 98217 98370 . - . gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local exon 98217 98370 . - . insertions 0 ; deletions 0 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice5 98215 98216 . - . intron_id 5 ; splice_site "GT" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local intron 92181 98216 . - . intron_id 5 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice3 92181 92182 . - . intron_id 4 ; splice_site "AG" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local cds 92078 92180 . - . gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local exon 92078 92180 . - . insertions 0 ; deletions 0 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice5 92076 92077 . - . intron_id 6 ; splice_site "GT" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local intron 88917 92077 . - . intron_id 6 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice3 88917 88918 . - . intron_id 5 ; splice_site "AG" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local cds 88787 88916 . - . gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local exon 88787 88916 . - . insertions 0 ; deletions 0 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice5 88785 88786 . - . intron_id 7 ; splice_site "GT" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local intron 88661 88786 . - . intron_id 7 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice3 88661 88662 . - . intron_id 6 ; splice_site "AG" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local cds 88569 88660 . - . gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local exon 88569 88660 . - . insertions 0 ; deletions 0 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice5 88567 88568 . - . intron_id 8 ; splice_site "GT" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local intron 68763 88568 . - . intron_id 8 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice3 68763 68764 . - . intron_id 7 ; splice_site "AG" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local cds 68669 68762 . - . gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local exon 68669 68762 . - . insertions 0 ; deletions 0 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice5 68667 68668 . - . intron_id 9 ; splice_site "GT" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local intron 68052 68668 . - . intron_id 9 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice3 68052 68053 . - . intron_id 8 ; splice_site "AG" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local cds 67961 68051 . - . gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local exon 67961 68051 . - . insertions 0 ; deletions 0 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice5 67959 67960 . - . intron_id 10 ; splice_site "GT" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local intron 66475 67960 . - . intron_id 10 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local splice3 66475 66476 . - . intron_id 9 ; splice_site "AG" gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local cds 66276 66474 . - . gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local exon 66276 66474 . - . insertions 0 ; deletions 0 gi|397463559|gb|JH731942.1|:subseq(600000,155000) exonerate:protein2genome:local similarity 66276 115575 2006 - . alignment_id 1 ; Query SPP00000065_2.0 ; Align 115576 1 114 ; Align 108686 39 153 ; Align 104577 91 117 ; Align 104363 131 156 ; Align 98369 184 150 ; Align 92180 235 102 ; Align 88917 269 129 ; Align 88659 313 90 ; Align 68763 343 93 ; Align 68050 375 87 ; Align 66474 405 198 # --- END OF GFF DUMP --- # -- completed exonerate analysis