Command line: [exonerate -m p2g --showtargetgff -q ../Sel.human/SECP43.fa -t gi|397461042|gb|JH734459.1|subseq.fa -E] Hostname: [ws101066] ** (process:3891): WARNING **: Exhaustively generating suboptimal alignments will be VERY SLOW C4 Alignment: ------------ Query: SPP00000146_2.0 # Protein # tRNA Sec 1 associated protein 1 (SECp43) # Human (Homo sapiens) Target: gi|397461042|gb|JH734459.1|:subseq(152395,112049) Alligator mississippiensis unplaced genomic scaffold scaffold-5587, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 1399 Query range: 0 -> 287 Target range: 62489 -> 49999 1 : MetAlaAlaSerLeuTrpMetGlyAsp >>>> Target Intron 1 >>>> LeuG : 11 ||||||||||||||||||||||||||| 413 bp |||| MetAlaAlaSerLeuTrpMetGlyAsp++ ++LeuG 62489 : ATGGCCGCCAGCCTCTGGATGGGAGACgt.........................agCTGG : 62046 12 : luProTyrMetAspGluAsnPheIleSerArgAlaPheAlaThrMetGlyGluThrValM : 31 |||||||||||||||||||||||:!!||||||||||||||||||||||||||| !|||: luProTyrMetAspGluAsnPheValSerArgAlaPheAlaThrMetGlyGluLeuValL 62045 : AGCCGTACATGGACGAGAACTTTGTTTCGAGAGCCTTCGCCACCATGGGAGAGCTTGTGC : 61986 32 : etSerValLysIleIleArgAsnArgLeuThr{Gl} >>>> Target Intron 2 > : 42 !!||||||||||||||||||||||||||||||{||} 1086 bp euSerValLysIleIleArgAsnArgLeuThr{Gl}++ 61985 : TGAGCGTGAAAATCATCCGCAATCGATTGACG{GG}gt...................... : 61949 43 : >>> {y}IleProAlaGlyTyrCysPheValGluPheAlaAspLeuAlaThrAlaGluL : 60 {|}|||||||||||||||||||||||||||||||||||||||||||||||||||| ++{y}IleProAlaGlyTyrCysPheValGluPheAlaAspLeuAlaThrAlaGluL 61948 : ...ag{A}ATTCCTGCGGGCTATTGCTTTGTGGAATTTGCAGATCTGGCTACTGCAGAGA : 60813 61 : ysCysLeuHisLysIleAsnGlyLysProLeuProGlyAlaThrPro >>>> Target : 76 ||||||||||||||||||||||||||||||||||||||||||||||| 24 ysCysLeuHisLysIleAsnGlyLysProLeuProGlyAlaThrPro++ 60812 : AATGTTTACACAAAATCAATGGAAAACCACTTCCTGGTGCCACACCAgt........... : 60763 77 : Intron 3 >>>> AlaLysArgPheLysLeuAsnTyrAlaThrTyrGlyLysGlnPr : 90 64 bp |||||||||||||||||||||||||||||||||||||||||||| ++AlaLysArgPheLysLeuAsnTyrAlaThrTyrGlyLysGlnPr 60762 : ..............agGCAAAACGATTTAAACTGAATTATGCAACATACGGAAAACAACC : 58259 91 : oAspAsn{Se} >>>> Target Intron 4 >>>> {r}ProGluTyrSerLeuPh : 99 |||||||{||} 1177 bp {|}||||||||||||||||| oAspAsn{Se}++ ++{r}ProGluTyrSerLeuPh 58258 : TGATAAC{AG}gt.........................ag{T}CCGGAATACTCGCTTTT : 57055 100 : eValGlyAspLeuThrProAspValAspAspGlyMetLeuTyrGluPhePheValLysVa : 119 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| eValGlyAspLeuThrProAspValAspAspGlyMetLeuTyrGluPhePheValLysVa 57054 : TGTGGGAGATTTGACCCCTGATGTGGATGATGGCATGCTATATGAGTTTTTTGTTAAAGT : 56995 120 : lTyrProSerCysArgGlyGlyLysValValLeuAspGlnThrGlyValSer{Ly} >> : 137 ||||||||||||||||||||||||||||||||||||||||:!!|||||||||{||} lTyrProSerCysArgGlyGlyLysValValLeuAspGlnSerGlyValSer{Ly}++ 56994 : TTACCCATCATGTAGAGGTGGTAAAGTTGTCTTGGACCAATCAGGAGTTTCC{AA}gt.. : 56937 138 : >> Target Intron 5 >>>> {s}GlyTyrGlyPheValLysPheThrAspGluLe : 148 1975 bp {|}|||||||||||||||||||||||||||||||| ++{s}GlyTyrGlyPheValLysPheThrAspGluLe 56936 : .......................ag{A}GGCTATGGGTTTGTGAAATTCACAGATGAACT : 54933 149 : uGluGlnLysArgAlaLeuThrGluCysGlnGlyAlaValGlyLeuGlySerLysProVa : 168 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||:! uGluGlnLysArgAlaLeuThrGluCysGlnGlyAlaValGlyLeuGlySerLysProIl 54932 : GGAACAGAAGAGAGCCCTGACAGAATGCCAAGGAGCTGTGGGGTTGGGCTCCAAACCTAT : 54873 169 : lArgLeuSerValAlaIleProLys{Al} >>>> Target Intron 6 >>>> {a : 177 !||||||||||||||||||||||||{||} 2233 bp {| eArgLeuSerValAlaIleProLys{Al}++ ++{a 54872 : ACGTTTGAGCGTGGCCATACCAAAA{GC}gt.........................ag{G : 52611 178 : }SerArgValLysProValGluTyrSerGlnMetTyrSerTyrSerTyrAsnGlnTyrTy : 197 }!:!||||||||| !!!.!||||||!:!|||||||||!:!|||!:!|||||||||||||| }AsnArgValLysThrAlaGluTyrAsnGlnMetTyrAsnTyrAsnTyrAsnGlnTyrTy 52610 : }AACCGTGTGAAGACAGCCGAGTACAATCAGATGTACAACTATAATTATAACCAATATTA : 52553 198 : rGlnGlnTyrGlnAsnTyrTyrAlaGlnTrpGlyTyrAspGlnAsnThrGlySerTyrSe : 217 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| rGlnGlnTyrGlnAsnTyrTyrAlaGlnTrpGlyTyrAspGlnAsnThrGlySerTyrSe 52552 : CCAACAATATCAGAACTACTATGCGCAGTGGGGATATGACCAGAACACAGGCAGTTACAG : 52493 218 : rTyrSerTyrProGlnTyrGlyTyrThrGlnSerThrMetGln >>>> Target Int : 232 ||||||||||||||||||||||||||||||||||||||||||| 609 b rTyrSerTyrProGlnTyrGlyTyrThrGlnSerThrMetGln++ 52492 : CTACAGCTACCCCCAGTATGGGTACACGCAGAGCACTATGCAGgt............... : 52446 233 : ron 7 >>>> ThrTyrGluGluValGlyAspAspAlaLeuGlu{A} >>>> Targe : 243 p ||||||||||||||||||!!:||||||||||||{|} 1 ++ThrTyrGluGluValGlyGluAspAlaLeuGlu{A}++ 52445 : ..........agACATATGAAGAAGTTGGTGAGGATGCATTGGAA{G}gt.......... : 51803 244 : t Intron 8 >>>> {sp}ProMetProGlnLeuAspValThrGluAlaAsnLysGlu : 256 672 bp {||}||| |||||||||||||||!:!||||||||||||:!! ++{sp}ProAlaProGlnLeuAspValSerGluAlaAsnLysGln 51802 : ...............ag{AT}CCAGCTCCTCAGTTAGATGTGAGTGAAGCAAACAAGCAG : 50095 257 : PheMetGluGlnSerGluGluLeuTyrAspAlaLeuMetAspCysHisTrpGlnProLeu : 276 |||:!:||||||||||||||||||||||||||||||! |||! !||||||||||||||| PheValGluGlnSerGluGluLeuTyrAspAlaLeuThrAspSerHisTrpGlnProLeu 50094 : TTTGTAGAACAGAGTGAAGAGCTTTATGATGCCTTGACTGACTCTCACTGGCAGCCTTTG : 50035 277 : AspThrValSerSerGluIleProAlaMetMet : 287 |||||||||||||||||||||||||||!!::!: AspThrValSerSerGluIleProAlaIleLeu 50034 : GACACCGTCTCTTCAGAGATTCCAGCCATCTTA : 50000 vulgar: SPP00000146_2.0 0 287 . gi|397461042|gb|JH734459.1|:subseq(152395,112049) 62489 49999 - 1399 M 9 27 5 0 2 I 0 409 3 0 2 M 32 96 S 0 2 5 0 2 I 0 1082 3 0 2 S 1 1 M 33 99 5 0 2 I 0 2460 3 0 2 M 17 51 S 0 2 5 0 2 I 0 1173 3 0 2 S 1 1 M 43 129 S 0 2 5 0 2 I 0 1971 3 0 2 S 1 1 M 39 117 S 0 2 5 0 2 I 0 2229 3 0 2 S 1 1 M 54 162 5 0 2 I 0 605 3 0 2 M 11 33 S 0 1 5 0 2 I 0 1668 3 0 2 S 1 2 M 44 132 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-25 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local gene 50000 62489 1399 - . gene_id 1 ; sequence SPP00000146_2.0 ; gene_orientation + gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local cds 62463 62489 . - . gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local exon 62463 62489 . - . insertions 0 ; deletions 0 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice5 62461 62462 . - . intron_id 1 ; splice_site "GT" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local intron 62050 62462 . - . intron_id 1 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice3 62050 62051 . - . intron_id 0 ; splice_site "AG" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local cds 61952 62049 . - . gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local exon 61952 62049 . - . insertions 0 ; deletions 0 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice5 61950 61951 . - . intron_id 2 ; splice_site "GT" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local intron 60866 61951 . - . intron_id 2 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice3 60866 60867 . - . intron_id 1 ; splice_site "AG" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local cds 60766 60865 . - . gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local exon 60766 60865 . - . insertions 0 ; deletions 0 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice5 60764 60765 . - . intron_id 3 ; splice_site "GT" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local intron 58302 60765 . - . intron_id 3 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice3 58302 58303 . - . intron_id 2 ; splice_site "AG" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local cds 58249 58301 . - . gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local exon 58249 58301 . - . insertions 0 ; deletions 0 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice5 58247 58248 . - . intron_id 4 ; splice_site "GT" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local intron 57072 58248 . - . intron_id 4 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice3 57072 57073 . - . intron_id 3 ; splice_site "AG" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local cds 56940 57071 . - . gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local exon 56940 57071 . - . insertions 0 ; deletions 0 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice5 56938 56939 . - . intron_id 5 ; splice_site "GT" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local intron 54965 56939 . - . intron_id 5 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice3 54965 54966 . - . intron_id 4 ; splice_site "AG" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local cds 54845 54964 . - . gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local exon 54845 54964 . - . insertions 0 ; deletions 0 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice5 54843 54844 . - . intron_id 6 ; splice_site "GT" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local intron 52612 54844 . - . intron_id 6 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice3 52612 52613 . - . intron_id 5 ; splice_site "AG" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local cds 52449 52611 . - . gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local exon 52449 52611 . - . insertions 0 ; deletions 0 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice5 52447 52448 . - . intron_id 7 ; splice_site "GT" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local intron 51840 52448 . - . intron_id 7 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice3 51840 51841 . - . intron_id 6 ; splice_site "AG" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local cds 51806 51839 . - . gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local exon 51806 51839 . - . insertions 0 ; deletions 0 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice5 51804 51805 . - . intron_id 8 ; splice_site "GT" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local intron 50134 51805 . - . intron_id 8 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local splice3 50134 50135 . - . intron_id 7 ; splice_site "AG" gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local cds 50000 50133 . - . gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local exon 50000 50133 . - . insertions 0 ; deletions 0 gi|397461042|gb|JH734459.1|:subseq(152395,112049) exonerate:protein2genome:local similarity 50000 62489 1399 - . alignment_id 1 ; Query SPP00000146_2.0 ; Align 62490 1 27 ; Align 62050 10 96 ; Align 60865 43 99 ; Align 58302 76 51 ; Align 57071 94 129 ; Align 54964 138 117 ; Align 52611 178 162 ; Align 51840 232 33 ; Align 50132 244 132 # --- END OF GFF DUMP --- # -- completed exonerate analysis