Command line: [exonerate -m p2g --showtargetgff -q ../Sel.human/PSTK.fa -t gi|397460868|gb|JH734633.1|subseq.fa -E] Hostname: [ws101067] C4 Alignment: ------------ Query: SPP00000052_2.0 # Protein # Phosphoseryl-tRNA kinase (PSTK) # Human (Homo sapiens) Target: gi|397460868|gb|JH734633.1|:subseq(120000,100000) Alligator mississippiensis unplaced genomic scaffold scaffold-5878, whole genome shotgun sequence Model: protein2genome:local Raw score: 725 Query range: 15 -> 336 Target range: 49708 -> 95000 16 : ArgLysArgGlyLeuCysValLeuCysGlyLeuProAlaAlaGlyLysSerThrPheAla : 35 ||| ! !|||||||||||||||||||||||||||||||||||||||!!!|||.!..!! ArgGlyLeuGlyLeuCysValLeuCysGlyLeuProAlaAlaGlyLysThrThrLeuThr 49709 : CGGGGCCTGGGTCTGTGCGTGTTGTGCGGGCTGCCCGCAGCGGGCAAGACCACGCTAACG : 49766 36 : ArgAlaLeuAlaHisArgLeuGlnGlnGluGlnGlyTrpAlaIleGlyValValAlaTyr : 55 !:! !||| !:!! !|||::!! !! !|||! ! ! ! !:!!:!!||| GlnArgLeu------LysGlnGlnArgGlyTrpAspTrpAspCysPheArgLeuSerTyr 49767 : CAGCGCCTG------AAGCAGCAGAGAGGCTGGGACTGGGATTGCTTCCGCCTTTCCTAC : 49820 56 : AspAspValMetPro<->AspAlaPheLeuAlaGlyAlaArgAlaArgProAla >>>> : 73 ||||||:!!!!:||| !!:|||||| ! !..!!.!! !!.! ! ! ! AspAspLeuIleProLeuGluAlaPheSerGlnSerValProGlyAlaGlyLeu+- 49821 : GATGACCTCATTCCGCTGGAGGCCTTCAGCCAATCAGTGCCGGGGGCGGGGCTTga.... : 49879 74 : Target Intron 1 >>>> ProSerGlnTrpLysLeuLeuArgGlnGluLeuLeuL : 85 1712 bp !|||!::||||||! !! !||||||||||||||| ++ValSerArgTrpLysSerTyrArgGlnGluLeuLeuV 49880 : .....................agGTGTCGCGCTGGAAATCATACCGGCAGGAACTGCTGG : 51625 86 : ysTyrLeuGluTyrPheLeuMetAlaValIleAsnGlyCysGlnMetSerValProProA : 105 !|||||||||:!!||||||..!|||:!!:!!|||||| !! :!!|||!.!||| !!! alTyrLeuGluHisPheLeuGlnAlaLeuLeuAsnGlyAspProLeuSerAlaProAlaS 51626 : TATACCTTGAACATTTCCTGCAGGCTCTTCTTAATGGGGATCCCCTGTCTGCCCCAGCAA : 51685 106 : snArgThrGluAlaMetTrpGluAspPheIleThrCysLeuLysAspGlnAspLeuIleP : 125 :!||||||||||||! |||||| !|||:!!:!!|||!!!!:!!!:|||! ||||||! erArgThrGluAlaThrTrpGluArgPheValSerCysPheArgGluGlnGlyLeuIleS 51686 : GCAGGACAGAAGCCACCTGGGAACGTTTTGTTTCCTGCTTCAGAGAACAAGGATTAATAT : 51745 126 : heSerAlaAlaPheGluAlaGlnSerCysTyrLeuLeuThrLysThrAlaValSerArgP : 145 !|||! !!.! !!!:|||:!!|||||| !! !:!!! !!!..!!.!!!.!||||||| erSerAspValProAspAlaLysSerCysArgTyrValIleAsnAlaThrAlaSerArgP 51746 : CTTCAGACGTACCTGATGCTAAATCTTGTCGTTATGTGATCAATGCCACCGCTTCCAGAC : 51805 146 : roLeuPheLeuValLeuAspAspAsnPheTyrTyrGlnSerMetArgTyrGluValTyrG : 165 |||||! !!!!:!!|||||||||||||||||||||||||||||||||||||||||||||| roLeuCysPheIleLeuAspAspAsnPheTyrTyrGlnSerMetArgTyrGluValTyrG 51806 : CACTGTGTTTCATTTTGGATGACAACTTTTATTATCAAAGCATGAGATACGAAGTATACC : 51865 166 : lnLeuAlaArgLys{T} >>>> Target Intron 2 >>>> {yr}SerLeuGlyP : 174 ||||||||||||||{|} 295 bp {||}|||||||||| lnLeuAlaArgLys{T}++ ++{yr}SerLeuGlyP 51866 : AGCTAGCTCGCAAA{T}gt.........................ag{AT}TCATTAGGCT : 52187 175 : heCysGlnLeuPheLeuAspCysProLeuGluThrCysLeuGlnArgAsnGlyGlnArgP : 194 |||||||||||||||||||||||||||||||| !||||||||||||||| !! !||| heCysGlnLeuPheLeuAspCysProLeuGluCysCysLeuGlnArgAsnArgValArgS 52188 : TCTGCCAGTTATTTTTAGACTGTCCACTTGAATGCTGCTTACAGAGGAATCGCGTGAGAA : 52247 195 : roGlnAlaLeuProProGluThrIleHisLeuMetGlyArgLysLeuGluLysProAsnP : 214 !||| !!|||||| !|||! !||| !||||||!.!!:!|||:!!||| !||||||! erGlnProLeuProAspGluIleIleCysLeuMetAlaLysLysIleGluAlaProAsnL 52248 : GTCAGCCCTTACCTGACGAGATCATATGTCTAATGGCAAAAAAGATAGAAGCACCAAATC : 52307 215 : roGluLysAsnAlaTrpGluHisAsnSerLeuThrIleProSerProAlaCysAlaSerG : 234 !!!:||||||.!!||||||!!.||||||||||||:!: !.!!! !! !! !.!!|||| euAspLysAsnThrTrpGluGlnAsnSerLeuThrLeuLysGlyLeuGluTyrThrSerG 52308 : TTGATAAAAATACCTGGGAACAAAATAGTCTCACTCTGAAAGGGCTTGAATACACTTCAG : 52367 235 : luAla{Se} >>>> Target Intron 3 >>>> {r}LeuGluValThrAspLeuL : 243 ||! !{!:} 422 bp {!}|||:!!|||! !..!|||| luAsp{As}++ ++{n}LeuGlnValIleSerLeuL 52368 : AAGAT{AA}gt.........................ag{T}TTACAGGTAATCAGCCTGT : 52816 244 : euLeuThrAlaLeuGluAsnProValLysTyrAlaGluAspAsnMetGluGlnLys >> : 262 ||:!!|||||||||:!!|||||||||||| ! !|||!!:|||!!:||||||||| euValThrAlaLeuLysAsnProValLysGlnIleGluGluAsnIleGluGlnLys++ 52817 : TGGTTACTGCTTTGAAAAATCCAGTGAAACAAATTGAAGAAAATATTGAGCAAAAGgt.. : 52875 263 : >> Target Intron 4 >>>> AspThrAspArgIleIleCysSerThrAsnIleLe : 273 564 bp ! .!!|||||| !||||||:!!.!!!:!||||| ++AlaAlaAspArgAlaIleCysAlaAlaSerIleLe 52876 : .......................agGCAGCAGATCGAGCAATCTGTGCAGCTAGTATCCT : 53470 274 : uHisLysThrAspGlnThrLeuArgArgIleValSerGlnThrMetLysGluAlaLys{G : 293 ||||:!!.!!|||||||||!!!|||||||||:!!|||||||||||||||!!:||||||{| uHisGlnAlaAspGlnThrPheArgArgIleIleSerGlnThrMetLysAspAlaLys{G 53471 : CCATCAAGCTGATCAGACTTTTAGACGAATTATTTCTCAAACAATGAAGGATGCAAAA{G : 53530 294 : } >>>> Target Intron 5 >>>> {ly}AsnGlnGluAla >>>> Target : 298 } 11254 bp {||}!!.|||||| ! 884 }++ ++{ly}LysGlnGluLys+- 53531 : }gt.........................ag{GT}AAGCAGGAAAAGga............ : 64801 299 : Intron 6 >>>> PheSerGluMetThrPheLysGlnArgTrpValArgAlaAsnHis : 312 3 bp !!!! ! ||||||...!!.!!! |||! ! !|||||| ++LysThrValTyrThrPheSerHisSerLysValLeu***AsnHis 64802 : .............agAAGACAGTATATACATTTTCCCACAGCAAAGTACTGTAGAATCAT : 73684 313 : AlaAlaIle{T} >>>> Target Intron 7 >>>> {rp}ArgIleIleLeuGly : 321 :!! !:!:{|} 10503 bp {||}!.! ! ! ! SerTyrLeu{T}++ ++{rp}HisTrpCysAspGln 73685 : TCATACCTG{T}gt.........................ag{GG}CACTGGTGCGACCAG : 84214 322 : AsnGluHis{Il} >>>> Target Intron 8 >>>> {e}LysCysArgSerAla : 330 :::! |||{ } 10739 bp {!}|||||||||!!! ! SerGlyHis{Ar}++ ++{g}LysCysArgCysTyr 84215 : TCAGGCCAT{CG}gt.........................ag{C}AAATGCAGATGCTAC : 94980 331 : LysValGlyTrpLeuGln : 336 |||||| !||||||::! LysValLeuTrpLeuArg 94981 : AAAGTCTTATGGCTCAGA : 95000 vulgar: SPP00000052_2.0 15 336 . gi|397460868|gb|JH734633.1|:subseq(120000,100000) 49708 95000 + 725 M 23 69 G 2 0 M 20 60 G 0 3 M 12 36 5 0 2 I 0 1708 3 0 2 M 97 291 S 0 1 5 0 2 I 0 291 3 0 2 S 1 2 M 65 195 S 0 2 5 0 2 I 0 418 3 0 2 S 1 1 M 25 75 5 0 2 I 0 560 3 0 2 M 31 93 S 0 1 5 0 2 I 0 11250 3 0 2 S 1 2 M 4 12 5 0 2 I 0 8839 3 0 2 M 18 54 S 0 1 5 0 2 I 0 10499 3 0 2 S 1 2 M 8 24 S 0 2 5 0 2 I 0 10735 3 0 2 S 1 1 M 11 33 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-25 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local gene 49709 95000 725 + . gene_id 1 ; sequence SPP00000052_2.0 ; gene_orientation + gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local cds 49709 49876 . + . gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local exon 49709 49876 . + . insertions 3 ; deletions 2 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice5 49877 49878 . + . intron_id 1 ; splice_site "GA" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local intron 49877 51588 . + . intron_id 1 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice3 51587 51588 . + . intron_id 0 ; splice_site "AG" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local cds 51589 51880 . + . gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local exon 51589 51880 . + . insertions 0 ; deletions 0 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice5 51881 51882 . + . intron_id 2 ; splice_site "GT" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local intron 51881 52175 . + . intron_id 2 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice3 52174 52175 . + . intron_id 1 ; splice_site "AG" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local cds 52176 52374 . + . gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local exon 52176 52374 . + . insertions 0 ; deletions 0 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice5 52375 52376 . + . intron_id 3 ; splice_site "GT" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local intron 52375 52796 . + . intron_id 3 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice3 52795 52796 . + . intron_id 2 ; splice_site "AG" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local cds 52797 52872 . + . gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local exon 52797 52872 . + . insertions 0 ; deletions 0 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice5 52873 52874 . + . intron_id 4 ; splice_site "GT" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local intron 52873 53436 . + . intron_id 4 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice3 53435 53436 . + . intron_id 3 ; splice_site "AG" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local cds 53437 53530 . + . gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local exon 53437 53530 . + . insertions 0 ; deletions 0 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice5 53531 53532 . + . intron_id 5 ; splice_site "GT" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local intron 53531 64784 . + . intron_id 5 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice3 64783 64784 . + . intron_id 4 ; splice_site "AG" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local cds 64785 64798 . + . gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local exon 64785 64798 . + . insertions 0 ; deletions 0 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice5 64799 64800 . + . intron_id 6 ; splice_site "GA" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local intron 64799 73641 . + . intron_id 6 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice3 73640 73641 . + . intron_id 5 ; splice_site "AG" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local cds 73642 73696 . + . gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local exon 73642 73696 . + . insertions 0 ; deletions 0 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice5 73697 73698 . + . intron_id 7 ; splice_site "GT" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local intron 73697 84199 . + . intron_id 7 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice3 84198 84199 . + . intron_id 6 ; splice_site "AG" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local cds 84200 84227 . + . gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local exon 84200 84227 . + . insertions 0 ; deletions 0 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice5 84228 84229 . + . intron_id 8 ; splice_site "GT" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local intron 84228 94966 . + . intron_id 8 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local splice3 94965 94966 . + . intron_id 7 ; splice_site "AG" gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local cds 94967 95000 . + . gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local exon 94967 95000 . + . insertions 0 ; deletions 0 gi|397460868|gb|JH734633.1|:subseq(120000,100000) exonerate:protein2genome:local similarity 49709 95000 725 + . alignment_id 1 ; Query SPP00000052_2.0 ; Align 49709 16 69 ; Align 49778 41 60 ; Align 49841 61 36 ; Align 51589 73 291 ; Align 52178 171 195 ; Align 52798 237 75 ; Align 53437 262 93 ; Align 64787 294 12 ; Align 73642 298 54 ; Align 84202 317 24 ; Align 94968 326 33 # --- END OF GFF DUMP --- # -- completed exonerate analysis