TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000052_2.0 # Protein # Phosphoseryl-tRNA kinase (PSTK) #
Human (Homo sapiens)
(348 letters)
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g
enome.fa
14,644 sequences; 2,174,243,242 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|397460868|gb|JH734633.1| Alligator mississippiensis unplaced ... 121 5e-26
gi|397458528|gb|JH736973.1| Alligator mississippiensis unplaced ... 37 1.00
gi|397458369|gb|JH737132.1| Alligator mississippiensis unplaced ... 36 2.2
gi|397462881|gb|JH732620.1| Alligator mississippiensis unplaced ... 35 6.5
gi|397455976|gb|JH739524.1| Alligator mississippiensis unplaced ... 34 8.5
>gi|397460868|gb|JH734633.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-5878, whole genome shotgun sequence
Length = 648398
Score = 121 bits (303), Expect = 5e-26
Identities = 57/96 (59%), Positives = 71/96 (73%)
Frame = +1
Query: 74 SQWKLLRQELLKYLEYFLMAVINGCQMSVPPNRTEAMWEDFITCLKDQDLIFSAAFEAQS 133
S+WK RQELL YLE+FL A++NG +S P +RTEA WE F++C ++Q LI S +A+S
Sbjct: 171592 SRWKSYRQELLVYLEHFLQALLNGDPLSAPASRTEATWERFVSCFREQGLISSDVPDAKS 171771
Query: 134 CYLLTKTAVSRPLFLVLDDNFYYQSMRYEVYQLARK 169
C + SRPL +LDDNFYYQSMRYEVYQLARK
Sbjct: 171772 CRYVINATASRPLCFILDDNFYYQSMRYEVYQLARK 171879
Score = 89.4 bits (220), Expect = 2e-16
Identities = 46/83 (55%), Positives = 51/83 (61%)
Frame = +2
Query: 171 SLGFCQLFLDCPLETCLQRNGQRPQALPPETIHLMGRKLEKPNPEKNAWEHNSLTIPSPA 230
SLGFCQLFLDCPLE CLQRN R Q LP E I LM +K+E PN +KN WE NSLT+
Sbjct: 172178 SLGFCQLFLDCPLECCLQRNRVRSQPLPDEIICLMAKKIEAPNLDKNTWEQNSLTLKGLE 172357
Query: 231 CASEASLEVTDLLLTALENPVKY 253
SE V L +N V Y
Sbjct: 172358 YTSEDKYVVVLKFLYTYQNLVNY 172426
Score = 53.9 bits (128), Expect = 1e-05
Identities = 30/64 (46%), Positives = 40/64 (62%)
Frame = +1
Query: 257 NMEQKDTDRIICSTNILHKTDQTLRRIVSQTMKEAKGNQEAFSEMTFKQRWVRANHAAIW 316
N+EQ DR IC+ +ILH+ DQT RRI+SQTMK+AKGN + V A+ +W
Sbjct: 173425 NLEQA-ADRAICAASILHQADQTFRRIISQTMKDAKGN----CSN*YLAEAVSASFPEMW 173589
Query: 317 RIIL 320
+ IL
Sbjct: 173590 KAIL 173601
Score = 48.1 bits (113), Expect = 6e-04
Identities = 25/47 (53%), Positives = 33/47 (70%), Gaps = 2/47 (4%)
Frame = +2
Query: 16 RKRGLCVLCGLPAAGKSTFARALAHRLQQEQGWAIGV--VAYDDVMP 60
R GLCVLCGLPAAGK+T L RL+Q++GW ++YDD++P
Sbjct: 169709 RGLGLCVLCGLPAAGKTT----LTQRLKQQRGWDWDCFRLSYDDLIP 169837
Score = 37.4 bits (85), Expect = 1.00
Identities = 17/26 (65%), Positives = 22/26 (84%)
Frame = +1
Query: 236 SLEVTDLLLTALENPVKYAEDNMEQK 261
SL+V LL+TAL+NPVK E+N+EQK
Sbjct: 172795 SLQVISLLVTALKNPVKQIEENIEQK 172872
>gi|397458528|gb|JH736973.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-9731, whole genome shotgun sequence
Length = 1638992
Score = 37.4 bits (85), Expect = 1.00
Identities = 33/131 (25%), Positives = 58/131 (44%), Gaps = 15/131 (11%)
Frame = +1
Query: 113 DFITCLKDQDLIFSAAFEAQSCYLLTKTAVSRPLFLVLDDNFYYQSMRYEVYQLARKYSL 172
DF+T + L E C+ + +S+P+ ++ N + +++ V+ L + L
Sbjct: 418459 DFVTLMTHVHLGVHYDSEIPFCFCAAERVISQPVGVL---NIFTP*VQHSVFVLVVLHPL 418629
Query: 173 GFCQLFLDCPLETCLQR--NGQRPQALPP---------ETIH----LMGRKLEKPNPEKN 217
FC LF P++ CLQ QR P T+H + +E+P+PE
Sbjct: 418630 VFCPLF*--PVQVCLQSFPTLQRMHFTPQFSIIHKVRQNTLHAHIQVTDEDIEEPSPEGR 418803
Query: 218 AWEHNSLTIPS 228
+ H S +IP+
Sbjct: 418804 HYPHPSTSIPT 418836
>gi|397458369|gb|JH737132.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-9989, whole genome shotgun sequence
Length = 133886
Score = 36.2 bits (82), Expect = 2.2
Identities = 22/53 (41%), Positives = 25/53 (47%)
Frame = -2
Query: 177 LFLDCPLETCLQRNGQRPQALPPETIHLMGRKLEKPNPEKNAWEHNSLTIPSP 229
L+L CPL L R G LP H GRK P+ AW NS +PSP
Sbjct: 42862 LWLQCPLG--LHRPGA*GWVLPGGHYHRCGRKYWHPHSSGTAWPTNSSPLPSP 42710
>gi|397462881|gb|JH732620.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-2545, whole genome shotgun sequence
Length = 627355
Score = 34.7 bits (78), Expect = 6.5
Identities = 29/79 (36%), Positives = 36/79 (45%)
Frame = +2
Query: 20 LCVLCGLPAAGKSTFARALAHRLQQEQGWAIGVVAYDDVMPDAFLAGARARPAPSQWKLL 79
L VLCGLP +GKS A L L E GW + VVA + AG RA L
Sbjct: 314588 LVVLCGLPGSGKSRRAEELRAALGAE-GWPVHVVAEGE-------AGGRA--------AL 314719
Query: 80 RQELLKYLEYFLMAVINGC 98
R E + L + V++ C
Sbjct: 314720 RAEAERRLGRRAVVVVDAC 314776
>gi|397455976|gb|JH739524.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-14042, whole genome shotgun sequence
Length = 173304
Score = 34.3 bits (77), Expect = 8.5
Identities = 18/61 (29%), Positives = 31/61 (50%)
Frame = +1
Query: 91 LMAVINGCQMSVPPNRTEAMWEDFITCLKDQDLIFSAAFEAQSCYLLTKTAVSRPLFLVL 150
L G + + P T+A+W + L L+ + A+ ++ LLT T+V +P +L L
Sbjct: 86266 LYCYTTGISIYLGPRATQAVWGAVVGQLAPPTLLIATAWSPRALGLLTATSVQQPPWLTL 86445
Query: 151 D 151
D
Sbjct: 86446 D 86448
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis
/genome.fa
Posted date: Oct 18, 2013 5:53 PM
Number of letters in database: 2,174,243,242
Number of sequences in database: 14,644
Lambda K H
0.321 0.134 0.417
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,121,320,402
Number of Sequences: 14644
Number of extensions: 20054762
Number of successful extensions: 96343
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 1198
Number of HSP's successfully gapped in prelim test: 1146
Number of HSP's that attempted gapping in prelim test: 84735
Number of HSP's gapped (non-prelim): 31943
length of query: 348
length of database: 724,747,747
effective HSP length: 130
effective length of query: 218
effective length of database: 722,844,027
effective search space: 157579997886
effective search space used: 157579997886
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 76 (33.9 bits)