TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000038_2.0 # Protein # Glutathione peroxidase 6 (GPx6) # Human (Homo sapiens) (221 letters) Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g enome.fa 14,644 sequences; 2,174,243,242 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|397463514|gb|JH731987.1| Alligator mississippiensis unplaced ... 112 1e-23 gi|397464367|gb|JH731134.1| Alligator mississippiensis unplaced ... 103 4e-21 gi|397459705|gb|JH735796.1| Alligator mississippiensis unplaced ... 98 3e-19 gi|397461646|gb|JH733855.1| Alligator mississippiensis unplaced ... 55 2e-06 gi|397460984|gb|JH734517.1| Alligator mississippiensis unplaced ... 50 5e-05 gi|397462837|gb|JH732664.1| Alligator mississippiensis unplaced ... 40 5e-04 gi|397458980|gb|JH736521.1| Alligator mississippiensis unplaced ... 34 4.9 gi|397459392|gb|JH736109.1| Alligator mississippiensis unplaced ... 33 8.4 >gi|397463514|gb|JH731987.1| Alligator mississippiensis unplaced genomic scaffold scaffold-1586, whole genome shotgun sequence Length = 466719 Score = 112 bits (280), Expect = 1e-23 Identities = 57/124 (45%), Positives = 77/124 (62%), Gaps = 1/124 (0%) Frame = +3 Query: 99 FPCNQFGKQEPGTNSEILLGLKYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTF-LKNSCP 157 FP G +E G +E L + C S V + G+ G +F L+NSCP Sbjct: 210225 FPALPEG*RERGEGTEGLHLPQGPCTPSSHV------QAGETGGHGGDACPSFPLQNSCP 210386 Query: 158 PTSDLLGSSSQLFWEPMKVHDIRWNFEKFLVGPDGVPVMHWFHQAPVSTVKSDILEYLKQ 217 P + G +LFWEP+K+HDI+WNFEKFLVGPDGVPVM W+H+A ++TVK+DI+EY+K Sbjct: 210387 PVVENFGDPKKLFWEPLKIHDIKWNFEKFLVGPDGVPVMRWYHRANIATVKNDIMEYMKA 210566 Query: 218 FNTH 221 + H Sbjct: 210567 QHPH 210578 Score = 95.1 bits (235), Expect = 2e-18 Identities = 55/126 (43%), Positives = 64/126 (50%), Gaps = 46/126 (36%) Frame = +1 Query: 79 YPELNALQEELKNFGVIVLAFPCNQFGKQEPGTNSEILLGLK------------------ 120 + ELNALQ EL +G+++L FP NQFGKQEPG N+EIL LK Sbjct: 209929 HAELNALQSELGPYGLVILGFPSNQFGKQEPGQNAEILPALK*VPEPGQPGPGWGLPRTG 210108 Query: 121 ----------------------------YVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFL 152 YV PG GF P+FQLF+KGDVNGEKEQKV+TFL Sbjct: 210109 PYMGPAPTCTGRGWKGARHPLLPFHFFRYVRPGGGFAPNFQLFQKGDVNGEKEQKVYTFL 210288 Query: 153 KNSCPP 158 K P Sbjct: 210289 KVRAHP 210306 Score = 72.8 bits (177), Expect = 1e-11 Identities = 32/56 (57%), Positives = 43/56 (76%) Frame = +1 Query: 24 KPQNRKVDCNKGVTGTIYEYGALTLNGEEYIQFKQFAGKHVLFVNVAAYXGLAAQY 79 +P +V+C V GT+Y+YGALT++GEEY+ FK++AGK +LFVNVA Y GL QY Sbjct: 209293 RPAVFQVNCYSNVGGTVYDYGALTIDGEEYVPFKRYAGKMILFVNVATY*GLTMQY 209460 >gi|397464367|gb|JH731134.1| Alligator mississippiensis unplaced genomic scaffold scaffold-234, whole genome shotgun sequence Length = 432364 Score = 103 bits (258), Expect = 4e-21 Identities = 51/114 (44%), Positives = 70/114 (61%), Gaps = 4/114 (3%) Frame = +1 Query: 108 EPGTNSEILLGLKYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLKNSCPPTSD----LL 163 E TN EILL LKYV PG+G+ P+F +FEK DVNG +FTFLK + P D L+ Sbjct: 176959 ENATNDEILLSLKYVRPGNGYEPNFTMFEKCDVNGANAHPLFTFLKEALPFPHDDPVALM 177138 Query: 164 GSSSQLFWEPMKVHDIRWNFEKFLVGPDGVPVMHWFHQAPVSTVKSDILEYLKQ 217 + + W P+ +DI WNFEKFL+GPDG+P + + ++ DI + L+Q Sbjct: 177139 TNPQYIIWSPVCRNDISWNFEKFLIGPDGIPFKRYSRRFETIKIQDDIEKLLQQ 177300 Score = 50.8 bits (120), Expect = 4e-05 Identities = 29/67 (43%), Positives = 38/67 (56%), Gaps = 1/67 (1%) Frame = +3 Query: 45 ALTLNGEEYIQFKQFAGKHVLFVNVAAYXGLAAQ-YPELNALQEELKNFGVIVLAFPCNQ 103 A L G E + G+ +L NVA+ G A+ + +LN LQE G++VL FPCNQ Sbjct: 173979 ARPLGGGEPLALGSLLGRVLLVSNVASL*GTTARDFTQLNELQERYGPRGLLVLGFPCNQ 174158 Query: 104 FGKQEPG 110 FG Q PG Sbjct: 174159 FGHQVPG 174179 >gi|397459705|gb|JH735796.1| Alligator mississippiensis unplaced genomic scaffold scaffold-7762, whole genome shotgun sequence Length = 1008463 Score = 97.8 bits (242), Expect = 3e-19 Identities = 58/128 (45%), Positives = 70/128 (54%), Gaps = 4/128 (3%) Frame = -3 Query: 93 GVIVLAFPCNQFGKQEPGTNSEILLGLKYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFL 152 GV VL+ P QE TN EIL LKYV PG GF P+F LF+K VNG+ VF +L Sbjct: 890453 GVFVLSCPL-----QENCTNEEILNSLKYVRPGGGFEPNFTLFQKCQVNGQDTHPVFAYL 890289 Query: 153 KNSCPPTSD----LLGSSSQLFWEPMKVHDIRWNFEKFLVGPDGVPVMHWFHQAPVSTVK 208 K P D LL L W P++ D+ WNFEKFLVGP+G P + + S V+ Sbjct: 890288 KAHLPAPVDNPAALLTDPRFLVWSPVQRADLTWNFEKFLVGPEGEPFKRYSPKFQTSHVE 890109 Query: 209 SDILEYLK 216 DI LK Sbjct: 890108 PDIQRLLK 890085 Score = 53.1 bits (126), Expect = 8e-06 Identities = 31/76 (40%), Positives = 43/76 (56%), Gaps = 1/76 (1%) Frame = -3 Query: 36 VTGTIYEYGALTLNGEEYIQFKQFAGKHVLFVNVAAYXGLAAQ-YPELNALQEELKNFGV 94 + + Y+ A+TL GE+ + F F G+ VL NVA+ G + Y +LN LQ + Sbjct: 892058 IAKSFYDLSAITLQGEK-VDFNNFRGRVVLIENVASL*GTTVRDYTQLNQLQARYPR-RL 891885 Query: 95 IVLAFPCNQFGKQEPG 110 +VL FPCNQFG Q G Sbjct: 891884 VVLGFPCNQFGYQVSG 891837 >gi|397461646|gb|JH733855.1| Alligator mississippiensis unplaced genomic scaffold scaffold-4572, whole genome shotgun sequence Length = 115602 Score = 55.5 bits (132), Expect = 2e-06 Identities = 36/93 (38%), Positives = 47/93 (50%), Gaps = 1/93 (1%) Frame = +2 Query: 65 LFVNVAAYXGLA-AQYPELNALQEELKNFGVIVLAFPCNQFGKQEPGTNSEILLGLKYVC 123 L VNVA+ G + Y L LQ +L + VLAFPCNQFG+QEP T+ EI + Sbjct: 12230 LVVNVASECGYTDSHYKALQQLQRDLGPYHFNVLAFPCNQFGQQEPDTDKEIENFAR--- 12400 Query: 124 PGSGFVPSFQLFEKGDVNGEKEQKVFTFLKNSC 156 + +F LF K V+G F +L C Sbjct: 12401 --KTYGATFPLFSKTAVSGAGANAAFKYLIGEC 12493 >gi|397460984|gb|JH734517.1| Alligator mississippiensis unplaced genomic scaffold scaffold-5685, whole genome shotgun sequence Length = 643167 Score = 50.4 bits (119), Expect = 5e-05 Identities = 37/96 (38%), Positives = 50/96 (52%), Gaps = 5/96 (5%) Frame = -3 Query: 62 KHVLFVNVAAYXGLAAQYPELN--ALQEELKNFG---VIVLAFPCNQFGKQEPGTNSEIL 116 K L VNVA+Y Q+ + N ALQE + FG VLAFPCNQFG+ EP ++ EI Sbjct: 563947 KASLVVNVASY----CQHTDKNYIALQELHREFGPSHFTVLAFPCNQFGESEPSSSQEIE 563780 Query: 117 LGLKYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFL 152 K + +F +F K + G + + F FL Sbjct: 563779 SFAK-----GNYGVTFPIFHKIKILGSEAEPAFKFL 563687 >gi|397462837|gb|JH732664.1| Alligator mississippiensis unplaced genomic scaffold scaffold-2624, whole genome shotgun sequence Length = 587981 Score = 39.7 bits (91), Expect(2) = 5e-04 Identities = 28/91 (30%), Positives = 46/91 (50%), Gaps = 6/91 (6%) Frame = -1 Query: 37 TGTIYEYGALTLNGEEYIQFKQFAGKHVLFVNVAAYXGLA-AQYPELNALQEELKNFGVI 95 T +IYE ++G ++ +++ G + N+A+ G Y +L + + Sbjct: 271811 TKSIYESHVKDIDGS-HVSLEKYWGYACIITNIASK*GKTDINYTQLVDMYAI*TERCLR 271635 Query: 96 VLAFPCNQFGKQEPGTNSEI-----LLGLKY 121 +LAFPCNQF KQ PG N++I G+KY Sbjct: 271634 ILAFPCNQFKKQ*PGNNAQIKAFVASYGMKY 271542 Score = 26.6 bits (57), Expect(2) = 5e-04 Identities = 17/58 (29%), Positives = 27/58 (46%) Frame = -2 Query: 135 FEKGDVNGEKEQKVFTFLKNSCPPTSDLLGSSSQLFWEPMKVHDIRWNFEKFLVGPDG 192 F K DV G+ ++ +L+ P LG++ I+WNF KFL+ +G Sbjct: 271531 FSKTDVYGDNTLPLWKWLQEQ-PKGRGTLGNA------------IKWNFTKFLIDNEG 271397 >gi|397458980|gb|JH736521.1| Alligator mississippiensis unplaced genomic scaffold scaffold-8967, whole genome shotgun sequence Length = 861763 Score = 33.9 bits (76), Expect = 4.9 Identities = 19/51 (37%), Positives = 26/51 (50%), Gaps = 1/51 (1%) Frame = -1 Query: 61 GKHVLFVNVAAYXGLA-AQYPELNALQEELKNFGVIVLAFPCNQFGKQEPG 110 G+ + NVA+ G Y + + G+ +LAFPCNQFGKQ G Sbjct: 287896 GQVCIITNVASK*GKTDVNYTQFVDMYARYAERGLRILAFPCNQFGKQVGG 287744 >gi|397459392|gb|JH736109.1| Alligator mississippiensis unplaced genomic scaffold scaffold-8285, whole genome shotgun sequence Length = 876837 Score = 33.1 bits (74), Expect = 8.4 Identities = 15/27 (55%), Positives = 17/27 (62%) Frame = +3 Query: 109 PGTNSEILLGLKYVCPGSGFVPSFQLF 135 PGTNSE+L GL C F+PS Q F Sbjct: 467547 PGTNSELLFGLGDCCNSEHFLPSVQKF 467627 Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis /genome.fa Posted date: Oct 18, 2013 5:53 PM Number of letters in database: 2,174,243,242 Number of sequences in database: 14,644 Lambda K H 0.322 0.139 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 641,851,359 Number of Sequences: 14644 Number of extensions: 10363099 Number of successful extensions: 40023 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 730 Number of HSP's successfully gapped in prelim test: 367 Number of HSP's that attempted gapping in prelim test: 37414 Number of HSP's gapped (non-prelim): 7639 length of query: 221 length of database: 724,747,747 effective HSP length: 124 effective length of query: 97 effective length of database: 722,931,891 effective search space: 70124393427 effective search space used: 70124393427 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 73 (32.7 bits)