TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000021_2.0 # Protein # Glutathione peroxidase 3 (GPx3) # Human (Homo sapiens) (226 letters) Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g enome.fa 14,644 sequences; 2,174,243,242 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|397463514|gb|JH731987.1| Alligator mississippiensis unplaced ... 115 1e-24 gi|397464367|gb|JH731134.1| Alligator mississippiensis unplaced ... 101 3e-20 gi|397459705|gb|JH735796.1| Alligator mississippiensis unplaced ... 97 5e-19 gi|397460984|gb|JH734517.1| Alligator mississippiensis unplaced ... 57 6e-07 gi|397461646|gb|JH733855.1| Alligator mississippiensis unplaced ... 57 6e-07 gi|397462837|gb|JH732664.1| Alligator mississippiensis unplaced ... 46 1e-05 gi|397458980|gb|JH736521.1| Alligator mississippiensis unplaced ... 39 0.16 gi|397457772|gb|JH737729.1| Alligator mississippiensis unplaced ... 35 3.0 gi|397460449|gb|JH735052.1| Alligator mississippiensis unplaced ... 33 6.7 >gi|397463514|gb|JH731987.1| Alligator mississippiensis unplaced genomic scaffold scaffold-1586, whole genome shotgun sequence Length = 466719 Score = 115 bits (288), Expect = 1e-24 Identities = 64/126 (50%), Positives = 69/126 (54%), Gaps = 46/126 (36%) Frame = +1 Query: 79 YIELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPTLK------------------ 120 + ELNALQ EL P+GLVILGFP NQFGKQEPG+N+EILP LK Sbjct: 209929 HAELNALQSELGPYGLVILGFPSNQFGKQEPGQNAEILPALK*VPEPGQPGPGWGLPRTG 210108 Query: 121 ----------------------------YVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFL 152 YVRPGGGF PNFQLF+KGDVNGEKEQK YTFL Sbjct: 210109 PYMGPAPTCTGRGWKGARHPLLPFHFFRYVRPGGGFAPNFQLFQKGDVNGEKEQKVYTFL 210288 Query: 153 KNSCPP 158 K P Sbjct: 210289 KVRAHP 210306 Score = 112 bits (281), Expect = 9e-24 Identities = 58/129 (44%), Positives = 78/129 (60%), Gaps = 1/129 (0%) Frame = +3 Query: 91 PFGLVILGFPCNQFGKQEPGENSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYT 150 P G + FP G +E GE +E L P G P+ + + G+ G + Sbjct: 210201 PGGRLRPQFPALPEG*RERGEGTEGLHL-----PQGPCTPSSHV-QAGETGGHGGDACPS 210362 Query: 151 F-LKNSCPPTSELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTTVSNVKM 209 F L+NSCPP E G +LFWEP+K+HDI+WNFEKFLVGPDG+P+MRW+HR ++ VK Sbjct: 210363 FPLQNSCPPVVENFGDPKKLFWEPLKIHDIKWNFEKFLVGPDGVPVMRWYHRANIATVKN 210542 Query: 210 DILSYMRRQ 218 DI+ YM+ Q Sbjct: 210543 DIMEYMKAQ 210569 Score = 83.2 bits (204), Expect = 7e-15 Identities = 34/52 (65%), Positives = 46/52 (88%) Frame = +1 Query: 29 KMDCHGGISGTIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASYXGLTGQYI 80 +++C+ + GT+Y+YGALTIDGEEY+PFK+YAGK +LFVNVA+Y GLT QY+ Sbjct: 209308 QVNCYSNVGGTVYDYGALTIDGEEYVPFKRYAGKMILFVNVATY*GLTMQYV 209463 >gi|397464367|gb|JH731134.1| Alligator mississippiensis unplaced genomic scaffold scaffold-234, whole genome shotgun sequence Length = 432364 Score = 101 bits (251), Expect = 3e-20 Identities = 50/108 (46%), Positives = 64/108 (59%), Gaps = 4/108 (3%) Frame = +1 Query: 108 EPGENSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTSE----LL 163 E N EIL +LKYVRPG G+ PNF +FEK DVNG +TFLK + P + L+ Sbjct: 176959 ENATNDEILLSLKYVRPGNGYEPNFTMFEKCDVNGANAHPLFTFLKEALPFPHDDPVALM 177138 Query: 164 GTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTTVSNVKMDI 211 + W P+ +DI WNFEKFL+GPDGIP R+ R ++ DI Sbjct: 177139 TNPQYIIWSPVCRNDISWNFEKFLIGPDGIPFKRYSRRFETIKIQDDI 177282 Score = 58.5 bits (140), Expect = 2e-07 Identities = 31/67 (46%), Positives = 40/67 (59%), Gaps = 1/67 (1%) Frame = +3 Query: 45 ALTIDGEEYIPFKQYAGKYVLFVNVASYXGLTGQ-YIELNALQEELAPFGLVILGFPCNQ 103 A + G E + G+ +L NVAS G T + + +LN LQE P GL++LGFPCNQ Sbjct: 173979 ARPLGGGEPLALGSLLGRVLLVSNVASL*GTTARDFTQLNELQERYGPRGLLVLGFPCNQ 174158 Query: 104 FGKQEPG 110 FG Q PG Sbjct: 174159 FGHQVPG 174179 >gi|397459705|gb|JH735796.1| Alligator mississippiensis unplaced genomic scaffold scaffold-7762, whole genome shotgun sequence Length = 1008463 Score = 97.1 bits (240), Expect = 5e-19 Identities = 54/123 (43%), Positives = 70/123 (56%), Gaps = 4/123 (3%) Frame = -3 Query: 93 GLVILGFPCNQFGKQEPGENSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFL 152 G+ +L P QE N EIL +LKYVRPGGGF PNF LF+K VNG+ + +L Sbjct: 890453 GVFVLSCPL-----QENCTNEEILNSLKYVRPGGGFEPNFTLFQKCQVNGQDTHPVFAYL 890289 Query: 153 KNSCPP----TSELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTTVSNVK 208 K P + LL L W P++ D+ WNFEKFLVGP+G P R+ + S+V+ Sbjct: 890288 KAHLPAPVDNPAALLTDPRFLVWSPVQRADLTWNFEKFLVGPEGEPFKRYSPKFQTSHVE 890109 Query: 209 MDI 211 DI Sbjct: 890108 PDI 890100 Score = 59.7 bits (143), Expect = 9e-08 Identities = 35/76 (46%), Positives = 47/76 (61%), Gaps = 1/76 (1%) Frame = -3 Query: 36 ISGTIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASYXGLTGQ-YIELNALQEELAPFGL 94 I+ + Y+ A+T+ GE+ + F + G+ VL NVAS G T + Y +LN LQ P L Sbjct: 892058 IAKSFYDLSAITLQGEK-VDFNNFRGRVVLIENVASL*GTTVRDYTQLNQLQARY-PRRL 891885 Query: 95 VILGFPCNQFGKQEPG 110 V+LGFPCNQFG Q G Sbjct: 891884 VVLGFPCNQFGYQVSG 891837 >gi|397460984|gb|JH734517.1| Alligator mississippiensis unplaced genomic scaffold scaffold-5685, whole genome shotgun sequence Length = 643167 Score = 57.0 bits (136), Expect = 6e-07 Identities = 35/92 (38%), Positives = 45/92 (48%), Gaps = 1/92 (1%) Frame = -3 Query: 62 KYVLFVNVASYXGLTGQ-YIELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPTLK 120 K L VNVASY T + YI L L E P +L FPCNQFG+ EP + EI K Sbjct: 563947 KASLVVNVASYCQHTDKNYIALQELHREFGPSHFTVLAFPCNQFGESEPSSSQEIESFAK 563768 Query: 121 YVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFL 152 G + F +F K + G + + + FL Sbjct: 563767 -----GNYGVTFPIFHKIKILGSEAEPAFKFL 563687 Score = 37.0 bits (84), Expect = 0.60 Identities = 18/55 (32%), Positives = 29/55 (52%) Frame = -2 Query: 163 LGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTTVSNVKMDILSYMRR 217 L SD + + K + RWNF K+LV P G + W + N+K ++ S +R+ Sbjct: 561719 LKLSDAILTDSSK-KEPRWNFWKYLVSPGGKVVKFWRPEEPIENIKPEVASLIRQ 561558 >gi|397461646|gb|JH733855.1| Alligator mississippiensis unplaced genomic scaffold scaffold-4572, whole genome shotgun sequence Length = 115602 Score = 57.0 bits (136), Expect = 6e-07 Identities = 37/93 (39%), Positives = 46/93 (49%), Gaps = 1/93 (1%) Frame = +2 Query: 65 LFVNVASYXGLT-GQYIELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPTLKYVR 123 L VNVAS G T Y L LQ +L P+ +L FPCNQFG+QEP + EI + R Sbjct: 12230 LVVNVASECGYTDSHYKALQQLQRDLGPYHFNVLAFPCNQFGQQEPDTDKEI---ENFAR 12400 Query: 124 PGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSC 156 G F LF K V+G + +L C Sbjct: 12401 KTYG--ATFPLFSKTAVSGAGANAAFKYLIGEC 12493 >gi|397462837|gb|JH732664.1| Alligator mississippiensis unplaced genomic scaffold scaffold-2624, whole genome shotgun sequence Length = 587981 Score = 46.2 bits (108), Expect(2) = 1e-05 Identities = 31/81 (38%), Positives = 44/81 (54%), Gaps = 4/81 (4%) Frame = -1 Query: 39 TIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASYXGLTG----QYIELNALQEELAPFGL 94 +IYE IDG ++ ++Y G + N+AS G T Q +++ A+ E L Sbjct: 271805 SIYESHVKDIDGS-HVSLEKYWGYACIITNIASK*GKTDINYTQLVDMYAI*TERC---L 271638 Query: 95 VILGFPCNQFGKQEPGENSEI 115 IL FPCNQF KQ PG N++I Sbjct: 271637 RILAFPCNQFKKQ*PGNNAQI 271575 Score = 25.8 bits (55), Expect(2) = 1e-05 Identities = 17/58 (29%), Positives = 25/58 (43%) Frame = -2 Query: 135 FEKGDVNGEKEQKFYTFLKNSCPPTSELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDG 192 F K DV G+ + +L+ P LG + I+WNF KFL+ +G Sbjct: 271531 FSKTDVYGDNTLPLWKWLQEQ-PKGRGTLGNA------------IKWNFTKFLIDNEG 271397 >gi|397458980|gb|JH736521.1| Alligator mississippiensis unplaced genomic scaffold scaffold-8967, whole genome shotgun sequence Length = 861763 Score = 38.9 bits (89), Expect = 0.16 Identities = 23/51 (45%), Positives = 27/51 (52%), Gaps = 1/51 (1%) Frame = -1 Query: 61 GKYVLFVNVASYXGLTG-QYIELNALQEELAPFGLVILGFPCNQFGKQEPG 110 G+ + NVAS G T Y + + A GL IL FPCNQFGKQ G Sbjct: 287896 GQVCIITNVASK*GKTDVNYTQFVDMYARYAERGLRILAFPCNQFGKQVGG 287744 >gi|397457772|gb|JH737729.1| Alligator mississippiensis unplaced genomic scaffold scaffold-10989, whole genome shotgun sequence Length = 519662 Score = 34.7 bits (78), Expect = 3.0 Identities = 14/33 (42%), Positives = 20/33 (60%) Frame = +3 Query: 130 PNFQLFEKGDVNGEKEQKFYTFLKNSCPPTSEL 162 PNF +E G V G+K+ FY FL + PP ++ Sbjct: 58356 PNFSXYENGSVLGQKKTIFYPFLSSLLPPCCKM 58454 >gi|397460449|gb|JH735052.1| Alligator mississippiensis unplaced genomic scaffold scaffold-6543, whole genome shotgun sequence Length = 659660 Score = 33.5 bits (75), Expect = 6.7 Identities = 12/19 (63%), Positives = 16/19 (84%) Frame = +2 Query: 98 GFPCNQFGKQEPGENSEIL 116 GFPCN+FG++ P ENS +L Sbjct: 593387 GFPCNEFGQEGPWENSRLL 593443 Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis /genome.fa Posted date: Oct 18, 2013 5:53 PM Number of letters in database: 2,174,243,242 Number of sequences in database: 14,644 Lambda K H 0.320 0.140 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 583,311,608 Number of Sequences: 14644 Number of extensions: 8969429 Number of successful extensions: 31451 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 426 Number of HSP's successfully gapped in prelim test: 274 Number of HSP's that attempted gapping in prelim test: 29926 Number of HSP's gapped (non-prelim): 4385 length of query: 226 length of database: 724,747,747 effective HSP length: 125 effective length of query: 101 effective length of database: 722,917,247 effective search space: 73014641947 effective search space used: 73014641947 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 74 (33.1 bits)