TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000019_2.0 # Protein # Glutathione peroxidase 2 (GPx2) # Human (Homo sapiens) (190 letters) Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g enome.fa 14,644 sequences; 2,174,243,242 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|397459705|gb|JH735796.1| Alligator mississippiensis unplaced ... 188 8e-47 gi|397464367|gb|JH731134.1| Alligator mississippiensis unplaced ... 169 4e-41 gi|397463514|gb|JH731987.1| Alligator mississippiensis unplaced ... 63 4e-19 gi|397460984|gb|JH734517.1| Alligator mississippiensis unplaced ... 59 1e-07 gi|397461646|gb|JH733855.1| Alligator mississippiensis unplaced ... 52 1e-05 gi|397462837|gb|JH732664.1| Alligator mississippiensis unplaced ... 47 3e-04 gi|397458980|gb|JH736521.1| Alligator mississippiensis unplaced ... 45 0.001 gi|397459862|gb|JH735639.1| Alligator mississippiensis unplaced ... 36 0.69 >gi|397459705|gb|JH735796.1| Alligator mississippiensis unplaced genomic scaffold scaffold-7762, whole genome shotgun sequence Length = 1008463 Score = 188 bits (478), Expect = 8e-47 Identities = 82/116 (70%), Positives = 101/116 (87%) Frame = -3 Query: 74 QENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSL 133 QENC NEEILNSLKYVRPGGG++P FTL QKC+VNGQ+ HPVFAYLK LP P D+P +L Sbjct: 890426 QENCTNEEILNSLKYVRPGGGFEPNFTLFQKCQVNGQDTHPVFAYLKAHLPAPVDNPAAL 890247 Query: 134 MTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVA 189 +TDP+ ++WSPV+R+D+ WNFEKFL+GPEGEPF+RYS F T ++EPDI+RLLK+A Sbjct: 890246 LTDPRFLVWSPVQRADLTWNFEKFLVGPEGEPFKRYSPKFQTSHVEPDIQRLLKLA 890079 Score = 125 bits (313), Expect = 1e-27 Identities = 61/74 (82%), Positives = 66/74 (89%) Frame = -3 Query: 1 MAFIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLXGTTTRDFTQLNELQCRFPRR 60 M IAKSFYDLSAI+L GEKVDFN FRGR VLIENVASL GTT RD+TQLN+LQ R+PRR Sbjct: 892067 MTPIAKSFYDLSAITLQGEKVDFNNFRGRVVLIENVASL*GTTVRDYTQLNQLQARYPRR 891888 Query: 61 LVVLGFPCNQFGHQ 74 LVVLGFPCNQFG+Q Sbjct: 891887 LVVLGFPCNQFGYQ 891846 >gi|397464367|gb|JH731134.1| Alligator mississippiensis unplaced genomic scaffold scaffold-234, whole genome shotgun sequence Length = 432364 Score = 169 bits (429), Expect = 4e-41 Identities = 73/113 (64%), Positives = 95/113 (84%) Frame = +1 Query: 75 ENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLM 134 EN N+EIL SLKYVRPG GY+P FT+ +KC+VNG N HP+F +LK+ LP+P+DDP +LM Sbjct: 176959 ENATNDEILLSLKYVRPGNGYEPNFTMFEKCDVNGANAHPLFTFLKEALPFPHDDPVALM 177138 Query: 135 TDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLK 187 T+P+ IIWSPV R+D++WNFEKFLIGP+G PF+RYSR F TI I+ DI++LL+ Sbjct: 177139 TNPQYIIWSPVCRNDISWNFEKFLIGPDGIPFKRYSRRFETIKIQDDIEKLLQ 177297 Score = 78.2 bits (191), Expect = 2e-13 Identities = 39/58 (67%), Positives = 45/58 (77%), Gaps = 1/58 (1%) Frame = +3 Query: 18 GEKVDFNTFRGRAVLIENVASLXGTTTRDFTQLNELQCRF-PRRLVVLGFPCNQFGHQ 74 GE + + GR +L+ NVASL GTT RDFTQLNELQ R+ PR L+VLGFPCNQFGHQ Sbjct: 173997 GEPLALGSLLGRVLLVSNVASL*GTTARDFTQLNELQERYGPRGLLVLGFPCNQFGHQ 174170 >gi|397463514|gb|JH731987.1| Alligator mississippiensis unplaced genomic scaffold scaffold-1586, whole genome shotgun sequence Length = 466719 Score = 63.2 bits (152), Expect(2) = 4e-19 Identities = 43/119 (36%), Positives = 51/119 (42%), Gaps = 47/119 (39%) Frame = +1 Query: 49 QLNELQCRF-PRRLVVLGFPCNQFGHQENCQNEEILNSLK-------------------- 87 +LN LQ P LV+LGFP NQFG QE QN EIL +LK Sbjct: 209935 ELNALQSELGPYGLVILGFPSNQFGKQEPGQNAEILPALK*VPEPGQPGPGWGLPRTGPY 210114 Query: 88 --------------------------YVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLK 120 YVRPGGG+ P F L QK +VNG+ E V+ +LK Sbjct: 210115 MGPAPTCTGRGWKGARHPLLPFHFFRYVRPGGGFAPNFQLFQKGDVNGEKEQKVYTFLK 210291 Score = 53.9 bits (128), Expect(2) = 4e-19 Identities = 21/52 (40%), Positives = 31/52 (59%) Frame = +3 Query: 136 DPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLK 187 DPK + W P++ D+ WNFEKFL+GP+G P R+ ++ DI +K Sbjct: 210408 DPKKLFWEPLKIHDIKWNFEKFLVGPDGVPVMRWYHRANIATVKNDIMEYMK 210563 >gi|397460984|gb|JH734517.1| Alligator mississippiensis unplaced genomic scaffold scaffold-5685, whole genome shotgun sequence Length = 643167 Score = 58.9 bits (141), Expect = 1e-07 Identities = 35/95 (36%), Positives = 47/95 (49%), Gaps = 1/95 (1%) Frame = -3 Query: 29 RAVLIENVASLXGTTTRDFTQLNELQCRF-PRRLVVLGFPCNQFGHQENCQNEEILNSLK 87 +A L+ NVAS T +++ L EL F P VL FPCNQFG E ++EI + K Sbjct: 563947 KASLVVNVASYCQHTDKNYIALQELHREFGPSHFTVLAFPCNQFGESEPSSSQEIESFAK 563768 Query: 88 YVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDK 122 G Y TF + K ++ G P F +L K Sbjct: 563767 -----GNYGVTFPIFHKIKILGSEAEPAFKFLVGK 563678 >gi|397461646|gb|JH733855.1| Alligator mississippiensis unplaced genomic scaffold scaffold-4572, whole genome shotgun sequence Length = 115602 Score = 52.0 bits (123), Expect = 1e-05 Identities = 35/89 (39%), Positives = 43/89 (48%), Gaps = 1/89 (1%) Frame = +2 Query: 32 LIENVASLXGTTTRDFTQLNELQCRF-PRRLVVLGFPCNQFGHQENCQNEEILNSLKYVR 90 L+ NVAS G T + L +LQ P VL FPCNQFG QE ++EI N + Sbjct: 12230 LVVNVASECGYTDSHYKALQQLQRDLGPYHFNVLAFPCNQFGQQEPDTDKEIENFARKT- 12406 Query: 91 PGGGYQPTFTLVQKCEVNGQNEHPVFAYL 119 Y TF L K V+G + F YL Sbjct: 12407 ----YGATFPLFSKTAVSGAGANAAFKYL 12481 >gi|397462837|gb|JH732664.1| Alligator mississippiensis unplaced genomic scaffold scaffold-2624, whole genome shotgun sequence Length = 587981 Score = 47.4 bits (111), Expect = 3e-04 Identities = 29/78 (37%), Positives = 40/78 (51%), Gaps = 1/78 (1%) Frame = -1 Query: 6 KSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLXGTTTRDFTQLNELQCRFPRR-LVVL 64 KS Y+ +DG V + G A +I N+AS G T ++TQL ++ R L +L Sbjct: 271808 KSIYESHVKDIDGSHVSLEKYWGYACIITNIASK*GKTDINYTQLVDMYAI*TERCLRIL 271629 Query: 65 GFPCNQFGHQENCQNEEI 82 FPCNQF Q N +I Sbjct: 271628 AFPCNQFKKQ*PGNNAQI 271575 >gi|397458980|gb|JH736521.1| Alligator mississippiensis unplaced genomic scaffold scaffold-8967, whole genome shotgun sequence Length = 861763 Score = 45.1 bits (105), Expect = 0.001 Identities = 23/49 (46%), Positives = 31/49 (63%), Gaps = 1/49 (2%) Frame = -1 Query: 27 RGRAVLIENVASLXGTTTRDFTQLNELQCRFPRR-LVVLGFPCNQFGHQ 74 RG+ +I NVAS G T ++TQ ++ R+ R L +L FPCNQFG Q Sbjct: 287899 RGQVCIITNVASK*GKTDVNYTQFVDMYARYAERGLRILAFPCNQFGKQ 287753 >gi|397459862|gb|JH735639.1| Alligator mississippiensis unplaced genomic scaffold scaffold-7502, whole genome shotgun sequence Length = 939111 Score = 36.2 bits (82), Expect = 0.69 Identities = 19/68 (27%), Positives = 34/68 (50%), Gaps = 7/68 (10%) Frame = +2 Query: 52 ELQCRFPRRLVVLGFPCNQFG-------HQENCQNEEILNSLKYVRPGGGYQPTFTLVQK 104 E++C FP V+L +Q G + Q+ ++ + K + G+QP F +++K Sbjct: 567980 EIKC-FPSYRVILSLSLSQMGTASIGEIKSNSIQHTQLSDGFKLQQANEGFQPVFYILEK 568156 Query: 105 CEVNGQNE 112 C +GQ E Sbjct: 568157 CPYHGQTE 568180 Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis /genome.fa Posted date: Oct 18, 2013 5:53 PM Number of letters in database: 2,174,243,242 Number of sequences in database: 14,644 Lambda K H 0.323 0.141 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 513,038,884 Number of Sequences: 14644 Number of extensions: 8688302 Number of successful extensions: 27394 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 590 Number of HSP's successfully gapped in prelim test: 305 Number of HSP's that attempted gapping in prelim test: 25672 Number of HSP's gapped (non-prelim): 4646 length of query: 190 length of database: 724,747,747 effective HSP length: 122 effective length of query: 68 effective length of database: 722,961,179 effective search space: 49161360172 effective search space used: 49161360172 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 72 (32.3 bits)