TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000017_2.0 # Protein # Glutathione peroxidase 1 (GPx1) # Human (Homo sapiens) (203 letters) Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g enome.fa 14,644 sequences; 2,174,243,242 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|397464367|gb|JH731134.1| Alligator mississippiensis unplaced ... 189 4e-47 gi|397459705|gb|JH735796.1| Alligator mississippiensis unplaced ... 171 1e-41 gi|397463514|gb|JH731987.1| Alligator mississippiensis unplaced ... 74 1e-23 gi|397461646|gb|JH733855.1| Alligator mississippiensis unplaced ... 67 3e-10 gi|397460984|gb|JH734517.1| Alligator mississippiensis unplaced ... 67 6e-10 gi|397458980|gb|JH736521.1| Alligator mississippiensis unplaced ... 53 8e-06 gi|397462837|gb|JH732664.1| Alligator mississippiensis unplaced ... 43 8e-05 >gi|397464367|gb|JH731134.1| Alligator mississippiensis unplaced genomic scaffold scaffold-234, whole genome shotgun sequence Length = 432364 Score = 189 bits (481), Expect = 4e-47 Identities = 86/119 (72%), Positives = 100/119 (84%) Frame = +1 Query: 85 ENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALM 144 ENA N+EIL SLKYVRPG G+EPNF +FEKC+VNGA AHPLF FL+EALP P DD ALM Sbjct: 176959 ENATNDEILLSLKYVRPGNGYEPNFTMFEKCDVNGANAHPLFTFLKEALPFPHDDPVALM 177138 Query: 145 TDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLSQGPSCA 203 T+P+ I WSPVCRND++WNFEKFL+GPDG+P +RYSRRF+TI I+ DIE LL Q PS A Sbjct: 177139 TNPQYIIWSPVCRNDISWNFEKFLIGPDGIPFKRYSRRFETIKIQDDIEKLLQQIPSNA 177315 Score = 108 bits (270), Expect = 1e-22 Identities = 50/64 (78%), Positives = 57/64 (89%) Frame = +3 Query: 21 ARPLAGGEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQ 80 ARPL GGEP++LGSL G+VLL+ NVASL GTT RD+TQ+NELQ R GPRGL+VLGFPCNQ Sbjct: 173979 ARPLGGGEPLALGSLLGRVLLVSNVASL*GTTARDFTQLNELQERYGPRGLLVLGFPCNQ 174158 Query: 81 FGHQ 84 FGHQ Sbjct: 174159 FGHQ 174170 >gi|397459705|gb|JH735796.1| Alligator mississippiensis unplaced genomic scaffold scaffold-7762, whole genome shotgun sequence Length = 1008463 Score = 171 bits (434), Expect = 1e-41 Identities = 80/128 (62%), Positives = 97/128 (75%) Frame = -3 Query: 69 RGLVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAF 128 +G+ VL P QEN NEEILNSLKYVRPGGGFEPNF LF+KC+VNG HP+FA+ Sbjct: 890456 QGVFVLSCPL-----QENCTNEEILNSLKYVRPGGGFEPNFTLFQKCQVNGQDTHPVFAY 890292 Query: 129 LREALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDI 188 L+ LPAP D+ AL+TDP+ + WSPV R D+ WNFEKFLVGP+G P +RYS +FQT + Sbjct: 890291 LKAHLPAPVDNPAALLTDPRFLVWSPVQRADLTWNFEKFLVGPEGEPFKRYSPKFQTSHV 890112 Query: 189 EPDIEALL 196 EPDI+ LL Sbjct: 890111 EPDIQRLL 890088 Score = 85.5 bits (210), Expect = 1e-15 Identities = 47/71 (66%), Positives = 54/71 (76%) Frame = -3 Query: 14 QSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVV 73 +S Y SA L G E V + RG+V+LIENVASL GTTVRDYTQ+N+LQ R PR LVV Sbjct: 892052 KSFYDLSAITLQG-EKVDFNNFRGRVVLIENVASL*GTTVRDYTQLNQLQARY-PRRLVV 891879 Query: 74 LGFPCNQFGHQ 84 LGFPCNQFG+Q Sbjct: 891878 LGFPCNQFGYQ 891846 >gi|397463514|gb|JH731987.1| Alligator mississippiensis unplaced genomic scaffold scaffold-1586, whole genome shotgun sequence Length = 466719 Score = 74.3 bits (181), Expect(2) = 1e-23 Identities = 44/121 (36%), Positives = 55/121 (45%), Gaps = 46/121 (38%) Frame = +1 Query: 56 YTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLK------------------ 97 + ++N LQ LGP GLV+LGFP NQFG QE +N EIL +LK Sbjct: 209929 HAELNALQSELGPYGLVILGFPSNQFGKQEPGQNAEILPALK*VPEPGQPGPGWGLPRTG 210108 Query: 98 ----------------------------YVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFL 129 YVRPGGGF PNF LF+K +VNG ++ FL Sbjct: 210109 PYMGPAPTCTGRGWKGARHPLLPFHFFRYVRPGGGFAPNFQLFQKGDVNGEKEQKVYTFL 210288 Query: 130 R 130 + Sbjct: 210289 K 210291 Score = 58.5 bits (140), Expect(2) = 1e-23 Identities = 27/56 (48%), Positives = 37/56 (66%), Gaps = 1/56 (1%) Frame = +3 Query: 146 DPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDI-EALLSQGP 200 DPK + W P+ +D+ WNFEKFLVGPDGVP+ R+ R ++ DI E + +Q P Sbjct: 210408 DPKKLFWEPLKIHDIKWNFEKFLVGPDGVPVMRWYHRANIATVKNDIMEYMKAQHP 210575 >gi|397461646|gb|JH733855.1| Alligator mississippiensis unplaced genomic scaffold scaffold-4572, whole genome shotgun sequence Length = 115602 Score = 67.4 bits (163), Expect = 3e-10 Identities = 40/92 (43%), Positives = 51/92 (55%) Frame = +2 Query: 38 KVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLK 97 +V L+ NVAS G T Y + +LQR LGP VL FPCNQFG QE ++EI N + Sbjct: 12221 QVSLVVNVASECGYTDSHYKALQQLQRDLGPYHFNVLAFPCNQFGQQEPDTDKEIENFAR 12400 Query: 98 YVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFL 129 + F LF K V+GAGA+ F +L Sbjct: 12401 KT-----YGATFPLFSKTAVSGAGANAAFKYL 12481 >gi|397460984|gb|JH734517.1| Alligator mississippiensis unplaced genomic scaffold scaffold-5685, whole genome shotgun sequence Length = 643167 Score = 66.6 bits (161), Expect = 6e-10 Identities = 36/92 (39%), Positives = 49/92 (53%) Frame = -3 Query: 38 KVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLK 97 K L+ NVAS T ++Y + EL R GP VL FPCNQFG E + ++EI + K Sbjct: 563947 KASLVVNVASYCQHTDKNYIALQELHREFGPSHFTVLAFPCNQFGESEPSSSQEIESFAK 563768 Query: 98 YVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFL 129 G + F +F K ++ G+ A P F FL Sbjct: 563767 -----GNYGVTFPIFHKIKILGSEAEPAFKFL 563687 >gi|397458980|gb|JH736521.1| Alligator mississippiensis unplaced genomic scaffold scaffold-8967, whole genome shotgun sequence Length = 861763 Score = 52.8 bits (125), Expect = 8e-06 Identities = 28/54 (51%), Positives = 34/54 (62%) Frame = -1 Query: 31 SLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQ 84 SL RG+V +I NVAS G T +YTQ ++ R RGL +L FPCNQFG Q Sbjct: 287914 SLPLPRGQVCIITNVASK*GKTDVNYTQFVDMYARYAERGLRILAFPCNQFGKQ 287753 >gi|397462837|gb|JH732664.1| Alligator mississippiensis unplaced genomic scaffold scaffold-2624, whole genome shotgun sequence Length = 587981 Score = 43.1 bits (100), Expect(2) = 8e-05 Identities = 28/79 (35%), Positives = 40/79 (50%) Frame = -1 Query: 14 QSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVV 73 +S+Y + + G VSL G +I N+AS G T +YTQ+ ++ R L + Sbjct: 271808 KSIYESHVKDIDGSH-VSLEKYWGYACIITNIASK*GKTDINYTQLVDMYAI*TERCLRI 271632 Query: 74 LGFPCNQFGHQENAKNEEI 92 L FPCNQF Q N +I Sbjct: 271631 LAFPCNQFKKQ*PGNNAQI 271575 Score = 25.4 bits (54), Expect(2) = 8e-05 Identities = 18/62 (29%), Positives = 25/62 (40%) Frame = -2 Query: 112 FEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGP 171 F K +V G PL+ +L+E P T N + WNF KFL+ Sbjct: 271531 FSKTDVYGDNTLPLWKWLQEQ---PKGRGTL--------------GNAIKWNFTKFLIDN 271403 Query: 172 DG 173 +G Sbjct: 271402 EG 271397 Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis /genome.fa Posted date: Oct 18, 2013 5:53 PM Number of letters in database: 2,174,243,242 Number of sequences in database: 14,644 Lambda K H 0.321 0.139 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 527,827,930 Number of Sequences: 14644 Number of extensions: 8696968 Number of successful extensions: 30962 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 589 Number of HSP's successfully gapped in prelim test: 346 Number of HSP's that attempted gapping in prelim test: 28988 Number of HSP's gapped (non-prelim): 5254 length of query: 203 length of database: 724,747,747 effective HSP length: 123 effective length of query: 80 effective length of database: 722,946,535 effective search space: 57835722800 effective search space used: 57835722800 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 73 (32.7 bits)