TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000017_2.0 # Protein # Glutathione peroxidase 1 (GPx1) #
Human (Homo sapiens)
         (203 letters)

Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g
enome.fa 
           14,644 sequences; 2,174,243,242 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|397464367|gb|JH731134.1| Alligator mississippiensis unplaced ...   189   4e-47
gi|397459705|gb|JH735796.1| Alligator mississippiensis unplaced ...   171   1e-41
gi|397463514|gb|JH731987.1| Alligator mississippiensis unplaced ...    74   1e-23
gi|397461646|gb|JH733855.1| Alligator mississippiensis unplaced ...    67   3e-10
gi|397460984|gb|JH734517.1| Alligator mississippiensis unplaced ...    67   6e-10
gi|397458980|gb|JH736521.1| Alligator mississippiensis unplaced ...    53   8e-06
gi|397462837|gb|JH732664.1| Alligator mississippiensis unplaced ...    43   8e-05

>gi|397464367|gb|JH731134.1| Alligator mississippiensis unplaced genomic
              scaffold scaffold-234, whole genome shotgun sequence
          Length = 432364

 Score =  189 bits (481), Expect = 4e-47
 Identities = 86/119 (72%), Positives = 100/119 (84%)
 Frame = +1

Query: 85     ENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALM 144
              ENA N+EIL SLKYVRPG G+EPNF +FEKC+VNGA AHPLF FL+EALP P DD  ALM
Sbjct: 176959 ENATNDEILLSLKYVRPGNGYEPNFTMFEKCDVNGANAHPLFTFLKEALPFPHDDPVALM 177138

Query: 145    TDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLSQGPSCA 203
              T+P+ I WSPVCRND++WNFEKFL+GPDG+P +RYSRRF+TI I+ DIE LL Q PS A
Sbjct: 177139 TNPQYIIWSPVCRNDISWNFEKFLIGPDGIPFKRYSRRFETIKIQDDIEKLLQQIPSNA 177315



 Score =  108 bits (270), Expect = 1e-22
 Identities = 50/64 (78%), Positives = 57/64 (89%)
 Frame = +3

Query: 21     ARPLAGGEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQ 80
              ARPL GGEP++LGSL G+VLL+ NVASL GTT RD+TQ+NELQ R GPRGL+VLGFPCNQ
Sbjct: 173979 ARPLGGGEPLALGSLLGRVLLVSNVASL*GTTARDFTQLNELQERYGPRGLLVLGFPCNQ 174158

Query: 81     FGHQ 84
              FGHQ
Sbjct: 174159 FGHQ 174170


>gi|397459705|gb|JH735796.1| Alligator mississippiensis unplaced genomic
              scaffold scaffold-7762, whole genome shotgun sequence
          Length = 1008463

 Score =  171 bits (434), Expect = 1e-41
 Identities = 80/128 (62%), Positives = 97/128 (75%)
 Frame = -3

Query: 69     RGLVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAF 128
              +G+ VL  P      QEN  NEEILNSLKYVRPGGGFEPNF LF+KC+VNG   HP+FA+
Sbjct: 890456 QGVFVLSCPL-----QENCTNEEILNSLKYVRPGGGFEPNFTLFQKCQVNGQDTHPVFAY 890292

Query: 129    LREALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDI 188
              L+  LPAP D+  AL+TDP+ + WSPV R D+ WNFEKFLVGP+G P +RYS +FQT  +
Sbjct: 890291 LKAHLPAPVDNPAALLTDPRFLVWSPVQRADLTWNFEKFLVGPEGEPFKRYSPKFQTSHV 890112

Query: 189    EPDIEALL 196
              EPDI+ LL
Sbjct: 890111 EPDIQRLL 890088



 Score = 85.5 bits (210), Expect = 1e-15
 Identities = 47/71 (66%), Positives = 54/71 (76%)
 Frame = -3

Query: 14     QSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVV 73
              +S Y  SA  L G E V   + RG+V+LIENVASL GTTVRDYTQ+N+LQ R  PR LVV
Sbjct: 892052 KSFYDLSAITLQG-EKVDFNNFRGRVVLIENVASL*GTTVRDYTQLNQLQARY-PRRLVV 891879

Query: 74     LGFPCNQFGHQ 84
              LGFPCNQFG+Q
Sbjct: 891878 LGFPCNQFGYQ 891846


>gi|397463514|gb|JH731987.1| Alligator mississippiensis unplaced genomic
              scaffold scaffold-1586, whole genome shotgun sequence
          Length = 466719

 Score = 74.3 bits (181), Expect(2) = 1e-23
 Identities = 44/121 (36%), Positives = 55/121 (45%), Gaps = 46/121 (38%)
 Frame = +1

Query: 56     YTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLK------------------ 97
              + ++N LQ  LGP GLV+LGFP NQFG QE  +N EIL +LK                  
Sbjct: 209929 HAELNALQSELGPYGLVILGFPSNQFGKQEPGQNAEILPALK*VPEPGQPGPGWGLPRTG 210108

Query: 98     ----------------------------YVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFL 129
                                          YVRPGGGF PNF LF+K +VNG     ++ FL
Sbjct: 210109 PYMGPAPTCTGRGWKGARHPLLPFHFFRYVRPGGGFAPNFQLFQKGDVNGEKEQKVYTFL 210288

Query: 130    R 130
              +
Sbjct: 210289 K 210291



 Score = 58.5 bits (140), Expect(2) = 1e-23
 Identities = 27/56 (48%), Positives = 37/56 (66%), Gaps = 1/56 (1%)
 Frame = +3

Query: 146    DPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDI-EALLSQGP 200
              DPK + W P+  +D+ WNFEKFLVGPDGVP+ R+  R     ++ DI E + +Q P
Sbjct: 210408 DPKKLFWEPLKIHDIKWNFEKFLVGPDGVPVMRWYHRANIATVKNDIMEYMKAQHP 210575


>gi|397461646|gb|JH733855.1| Alligator mississippiensis unplaced genomic
             scaffold scaffold-4572, whole genome shotgun sequence
          Length = 115602

 Score = 67.4 bits (163), Expect = 3e-10
 Identities = 40/92 (43%), Positives = 51/92 (55%)
 Frame = +2

Query: 38    KVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLK 97
             +V L+ NVAS  G T   Y  + +LQR LGP    VL FPCNQFG QE   ++EI N  +
Sbjct: 12221 QVSLVVNVASECGYTDSHYKALQQLQRDLGPYHFNVLAFPCNQFGQQEPDTDKEIENFAR 12400

Query: 98    YVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFL 129
                    +   F LF K  V+GAGA+  F +L
Sbjct: 12401 KT-----YGATFPLFSKTAVSGAGANAAFKYL 12481


>gi|397460984|gb|JH734517.1| Alligator mississippiensis unplaced genomic
              scaffold scaffold-5685, whole genome shotgun sequence
          Length = 643167

 Score = 66.6 bits (161), Expect = 6e-10
 Identities = 36/92 (39%), Positives = 49/92 (53%)
 Frame = -3

Query: 38     KVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLK 97
              K  L+ NVAS    T ++Y  + EL R  GP    VL FPCNQFG  E + ++EI +  K
Sbjct: 563947 KASLVVNVASYCQHTDKNYIALQELHREFGPSHFTVLAFPCNQFGESEPSSSQEIESFAK 563768

Query: 98     YVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFL 129
                   G +   F +F K ++ G+ A P F FL
Sbjct: 563767 -----GNYGVTFPIFHKIKILGSEAEPAFKFL 563687


>gi|397458980|gb|JH736521.1| Alligator mississippiensis unplaced genomic
              scaffold scaffold-8967, whole genome shotgun sequence
          Length = 861763

 Score = 52.8 bits (125), Expect = 8e-06
 Identities = 28/54 (51%), Positives = 34/54 (62%)
 Frame = -1

Query: 31     SLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQ 84
              SL   RG+V +I NVAS  G T  +YTQ  ++  R   RGL +L FPCNQFG Q
Sbjct: 287914 SLPLPRGQVCIITNVASK*GKTDVNYTQFVDMYARYAERGLRILAFPCNQFGKQ 287753


>gi|397462837|gb|JH732664.1| Alligator mississippiensis unplaced genomic
              scaffold scaffold-2624, whole genome shotgun sequence
          Length = 587981

 Score = 43.1 bits (100), Expect(2) = 8e-05
 Identities = 28/79 (35%), Positives = 40/79 (50%)
 Frame = -1

Query: 14     QSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVV 73
              +S+Y    + + G   VSL    G   +I N+AS  G T  +YTQ+ ++      R L +
Sbjct: 271808 KSIYESHVKDIDGSH-VSLEKYWGYACIITNIASK*GKTDINYTQLVDMYAI*TERCLRI 271632

Query: 74     LGFPCNQFGHQENAKNEEI 92
              L FPCNQF  Q    N +I
Sbjct: 271631 LAFPCNQFKKQ*PGNNAQI 271575



 Score = 25.4 bits (54), Expect(2) = 8e-05
 Identities = 18/62 (29%), Positives = 25/62 (40%)
 Frame = -2

Query: 112    FEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGP 171
              F K +V G    PL+ +L+E    P    T                N + WNF KFL+  
Sbjct: 271531 FSKTDVYGDNTLPLWKWLQEQ---PKGRGTL--------------GNAIKWNFTKFLIDN 271403

Query: 172    DG 173
              +G
Sbjct: 271402 EG 271397


  Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis
  /genome.fa
    Posted date:  Oct 18, 2013  5:53 PM
  Number of letters in database: 2,174,243,242
  Number of sequences in database:  14,644
  
Lambda     K      H
   0.321    0.139    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 527,827,930
Number of Sequences: 14644
Number of extensions: 8696968
Number of successful extensions: 30962
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 589
Number of HSP's successfully gapped in prelim test: 346
Number of HSP's that attempted gapping in prelim test: 28988
Number of HSP's gapped (non-prelim): 5254
length of query: 203
length of database: 724,747,747
effective HSP length: 123
effective length of query: 80
effective length of database: 722,946,535
effective search space: 57835722800
effective search space used: 57835722800
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 73 (32.7 bits)