Command line: [exonerate -m p2g --showtargetgff -q ../Sel.human/EEFSEC.fa -t gi|397462090|gb|JH733411.1|subseq.fa -E] Hostname: [ws101065] C4 Alignment: ------------ Query: SPP00000144_2.0 # Protein # Eukaryotic elongation factor (eEFSec) # Human (Homo sapiens) Target: gi|397462090|gb|JH733411.1|:subseq(110221,245320) Alligator mississippiensis unplaced genomic scaffold scaffold-3859, whole genome shotgun sequence Model: protein2genome:local Raw score: 105 Query range: 179 -> 438 Target range: 8247 -> 244565 180 : ProIleIleProValAlaAlaLysProGlyGlyProGluAlaProGluThrGluAlaP : 199 ||| !||| !!:!! !! ! ||||||..! !.!. !||| !.!!!:|||| ProProIleSerMetProLeuCysProGlySerCysHisMetProThrAsnAspAlaP 8248 : CCTCCAATTTCCATGCCACTCTGTCCAGGCTCTTGTCACATGCCCACCAATGATGCAC : 8305 200 : roGln{G} >>>> Target Intron 1 >>>> {ly}IleProGluLeuIleGl : 207 ||:!!{|} 10742 bp {||}! !|||!!::!!.!!:! roLys{G}++ ++{ly}ThrProAspIlePheGl 8306 : CTAAG{G}gt.........................ag{GA}ACACCTGATATCTTTCA : 19071 208 : uLeuLeuThrSer >>>> Target Intron 2 >>>> GlnIleSerIleProT : 217 !||||||! !!:! 20938 bp !!.:!:..! ! !! nLeuLeuArgAsn++ ++HisValGlnTyrSerM 19072 : ACTGCTGAGAAATgt.........................agCACGTGCAATACAGCA : 40039 218 : hrArgAspPro{Se} >>>> Target Intron 3 >>>> {r}GlyProPheLe : 225 !|||||| !{!.} 4473 bp {!}||| !!|||! etArgAspSer{Ly}++ ++{s}GlySerPheSe 40040 : TGCGTGATAGC{AA}gt.........................ag{A}GGCTCCTTCTC : 44536 226 : uMetSerValAspHisCysPheSer >>>> Target Intron 4 >>>> IleL : 235 !! ||| !..!:!!|||!:!!.! 9363 bp :!:| rLysSerTyrSerTyrCysTyrLys++ -+LeuL 44537 : AAAATCTTACTCCTACTGTTATAAAgt.........................cgCTGA : 53929 236 : ysGlyGlnGlyThrValMetThrGlyThrIleLeuSerGlySerIleSerLeuGlyAs : 254 ||..!!:!|||!.!||| ! !! !:!!:!:! !||| !:!!! !! !:!!||||| ysSerArgGlyAsnValTrpProGlnSerLeuSerSerGlnAlaThrTyrValGlyAs 53930 : AGTCACGGGGGAACGTGTGGCCTCAGTCCCTGTCAAGTCAGGCCACATATGTAGGTGA : 53986 255 : pSerValGluIleProAlaLeuLysValValLysLysVal >>>> Target Intr : 268 | !:!! !! !||| !|||!:! ! ! !! ! 11175 b pHisMet***AlaProGlnLeuArgLysAsn***PheGln++ 53987 : CCACATGTAGGCACCACAATTAAGAAAAAATTAATTTCAAgt................ : 54030 269 : on 5 >>>> LysSerMetGlnMetPheHisMetProIleThrSerAlaMetGlnGl : 283 p !|||:!! :!: !|||||| !!|||:!!:!! !|||!!. ++ProSerValCysLeuProHisMetSerIleSerAlaAsnMetHisHi 54031 : .........agCCAAGCGTGTGCTTACCTCATATGTCCATCTCTGCAAATATGCATCA : 65248 284 : yAspArgLeuGlyIleCysValThrGlnPheAspProLysLeuLeuGlu{Ar} >>> : 300 ! ! !!||| ! !|||:!! ! .!.! !!!!.||||||! {||} sPro***LeuThrAlaCysLeuGlyAlaMetGlyAlaAsnLeuLeuGly{Ar}++ 65249 : TCCCTGACTTACAGCATGCCTCGGCGCTATGGGGGCCAACCTTCTTGGC{AG}gt... : 65303 301 : > Target Intron 6 >>>> {g} >>>> Target Intron 7 >>>> Gl : 301 17134 bp {|} 2391 bp || ++{g}++ ++Gl 65304 : ......................ag{G}gt.........................agGG : 84827 302 : yLeuValCysAlaProGlu{S} >>>> Target Intron 8 >>>> {er}Leu : 309 |||| !|||..!||| !{|} 18110 bp {||}:!! yLeuProCysCysProLeu{S}-+ ++{er}Ile 84828 : CCTGCCCTGCTGCCCCCTA{A}tt.........................ag{GT}ATA : 102961 310 : HisThrVal{Hi} >>>> Target Intron 9 >>>> {s}AlaAlaLeuIleS : 318 ||| !:!!{ } 7573 bp { }..! !! !:!!| HisGlyMet{Gl}++ ++{y}CysProSerValS 102962 : CATGGAATG{GG}gt.........................ag{A}TGTCCCAGTGTAT : 110561 319 : erValGluLysIleProTyrPheArgGlyProLeuGlnThrLysAlaLysPheHisIl : 337 || !..!|||:!!||||||.!. ! !! !||| ! ||| !! !!:! ! ! erTyrArgLysValProTyrLeuSerHisLeuLeuTyrThrCysMetThrTyrLysTh 110562 : CTTACAGAAAAGTACCATATCTGTCTCACCTTTTATATACCTGCATGACATACAAGAC : 110618 338 : eThrValGlyHis{G} >>>> Target Intron 10 >>>> {lu}ThrValMe : 345 !|||||| !! ! {.} 15290 bp {..}|||:!!:! rThrValTrpLys{A}-+ ++{rg}ThrMetLe 110619 : AACGGTCTGGAAA{C}at..........................ag{GC}ACAATGCT : 125932 346 : tGlyArgLeuMetPhePheSerProAlaProAspAsnPheAspGlnGluPro >>>> : 363 :! ! ! !|||||||||!:! !!||| !! !!!.. ! !|||:!! ! uGluLeuAlaMetPhePheAsnThrAlaThrHisThrHisIleGlnGlnLys++ 125933 : TGAGTTGGCAATGTTTTTCAACACGGCCACACACACACACATCCAGCAAAAGgt.... : 125988 364 : Target Intron 11 >>>> IleLeuAspSerPheAsnPheSerGlnGluTyrL : 374 5657 bp !:!! !.! !! !||| !!.!.... !| ++GluValMetLysHisIlePheProAspSerIleL 125989 : ......................agGAAGTTATGAAACACATTTTCCCTGACAGCATTC : 131676 375 : euPheGlnGluGlnTyrLeuSerLys{A} >>>> Target Intron 12 >>>> : 383 ||!::::!:!!...! ||| !|||{|} 29224 bp euTrpArgLysSerSerLeuHisLys{A}++ + 131677 : TCTGGAGAAAAAGTTCACTTCATAAA{G}gt..........................a : 160926 384 : {sp}LeuThrPro{Al} >>>> Target Intron 13 >>>> {a}ValThrA : 390 {||} !.!!|||{ } 39314 bp {!} !.!!| +{sp}ThrAlaPro{Tr}++ ++{p}LysAlaA 160927 : g{AT}ACTGCCCCA{TG}gt..........................ag{G}AAAGCTG : 200262 391 : spAsnAspGluAlaAspLysLysAlaGlyGlnAlaThrGluGlyHisCysProArgGl : 409 || !|||:!! ! !:! ||||||:!!||| !..!! !||| !||||||:: spPheAspLysLeuArgArgLeuAlaGlyGluAlaLeuArgAspHisAspProArgAr 200263 : ACTTTGACAAGCTCAGGAGGCTTGCTGGTGAGGCCCTAAGGGATCACGACCCAAGGAG : 200319 410 : n >>>> Target Intron 14 >>>> GlnTrpAlaLeu >>>> Target I : 414 ! 28071 bp :!! ! !:!! 765 g+- ++LysHisGluMet++ 200320 : Ggg..........................agAAACATGAGATGgt............. : 228407 415 : ntron 15 >>>> ValGluPheGluLysProVal >>>> Target Intron 1 : 421 5 bp |||:!! !!:!!||||||:!! 8431 bp -+ValLysValLysLysProMet++ 228408 : .............ggGTTAAAGTTAAAAAACCCATGgt.................... : 236083 422 : 6 >>>> ThrCysProArgLeuCysLeuValIleGlySerArgLeuAspAlaAspIl : 437 !|||||||||||||||||| !:!: !! ! !!|||..!|||..!:! -+LeuCysProArgLeuCysLeuHisLeuCysValCysLeuSerAlaSerLe 236084 : ......tgCTGTGTCCACGTCTGTGTCTGCATCTGTGTGTTTGTCTGTCTGCATCTCT : 244560 438 : eHis : 438 :||| uHis 244561 : GCAC : 244565 vulgar: SPP00000144_2.0 179 438 . gi|397462090|gb|JH733411.1|:subseq(110221,245320) 8247 244565 + 105 M 21 63 S 0 1 5 0 2 I 0 10738 3 0 2 S 1 2 M 10 30 5 0 2 I 0 20934 3 0 2 M 9 27 S 0 2 5 0 2 I 0 4469 3 0 2 S 1 1 M 12 36 5 0 2 I 0 9359 3 0 2 M 34 102 5 0 2 I 0 11171 3 0 2 M 32 96 S 0 2 5 0 2 I 0 17130 3 0 2 S 1 1 5 0 2 I 0 2387 3 0 2 M 7 21 S 0 1 5 0 2 I 0 18106 3 0 2 S 1 2 M 4 12 S 0 2 5 0 2 I 0 7569 3 0 2 S 1 1 M 28 84 S 0 1 5 0 2 I 0 15286 3 0 2 S 1 2 M 20 60 5 0 2 I 0 5653 3 0 2 M 20 60 S 0 1 5 0 2 I 0 29220 3 0 2 S 1 2 M 3 9 S 0 2 5 0 2 I 0 39310 3 0 2 S 1 1 M 22 66 5 0 2 I 0 28067 3 0 2 M 4 12 5 0 2 I 0 7651 3 0 2 M 7 21 5 0 2 I 0 8427 3 0 2 M 18 54 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-25 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local gene 8248 244565 105 + . gene_id 1 ; sequence SPP00000144_2.0 ; gene_orientation + gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 8248 8311 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 8248 8311 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 8312 8313 . + . intron_id 1 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 8312 19053 . + . intron_id 1 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 19052 19053 . + . intron_id 0 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 19054 19085 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 19054 19085 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 19086 19087 . + . intron_id 2 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 19086 40023 . + . intron_id 2 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 40022 40023 . + . intron_id 1 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 40024 40052 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 40024 40052 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 40053 40054 . + . intron_id 3 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 40053 44525 . + . intron_id 3 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 44524 44525 . + . intron_id 2 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 44526 44562 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 44526 44562 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 44563 44564 . + . intron_id 4 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 44563 53925 . + . intron_id 4 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 53924 53925 . + . intron_id 3 ; splice_site "CG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 53926 54027 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 53926 54027 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 54028 54029 . + . intron_id 5 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 54028 65202 . + . intron_id 5 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 65201 65202 . + . intron_id 4 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 65203 65300 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 65203 65300 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 65301 65302 . + . intron_id 6 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 65301 82434 . + . intron_id 6 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 82433 82434 . + . intron_id 5 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 82435 82435 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 82435 82435 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 82436 82437 . + . intron_id 7 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 82436 84826 . + . intron_id 7 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 84825 84826 . + . intron_id 6 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 84827 84848 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 84827 84848 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 84849 84850 . + . intron_id 8 ; splice_site "TT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 84849 102958 . + . intron_id 8 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 102957 102958 . + . intron_id 7 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 102959 102974 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 102959 102974 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 102975 102976 . + . intron_id 9 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 102975 110547 . + . intron_id 9 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 110546 110547 . + . intron_id 8 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 110548 110633 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 110548 110633 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 110634 110635 . + . intron_id 10 ; splice_site "AT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 110634 125923 . + . intron_id 10 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 125922 125923 . + . intron_id 9 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 125924 125985 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 125924 125985 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 125986 125987 . + . intron_id 11 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 125986 131642 . + . intron_id 11 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 131641 131642 . + . intron_id 10 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 131643 131703 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 131643 131703 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 131704 131705 . + . intron_id 12 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 131704 160927 . + . intron_id 12 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 160926 160927 . + . intron_id 11 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 160928 160940 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 160928 160940 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 160941 160942 . + . intron_id 13 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 160941 200254 . + . intron_id 13 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 200253 200254 . + . intron_id 12 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 200255 200321 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 200255 200321 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 200322 200323 . + . intron_id 14 ; splice_site "GG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 200322 228392 . + . intron_id 14 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 228391 228392 . + . intron_id 13 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 228393 228404 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 228393 228404 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 228405 228406 . + . intron_id 15 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 228405 236059 . + . intron_id 15 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 236058 236059 . + . intron_id 14 ; splice_site "GG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 236060 236080 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 236060 236080 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 236081 236082 . + . intron_id 16 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 236081 244511 . + . intron_id 16 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 244510 244511 . + . intron_id 15 ; splice_site "TG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 244512 244565 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 244512 244565 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local similarity 8248 244565 105 + . alignment_id 1 ; Query SPP00000144_2.0 ; Align 8248 180 63 ; Align 19056 202 30 ; Align 40024 212 27 ; Align 44527 222 36 ; Align 53926 234 102 ; Align 65203 268 96 ; Align 84827 301 21 ; Align 102961 309 12 ; Align 110549 314 84 ; Align 125926 343 60 ; Align 131643 363 60 ; Align 160930 384 9 ; Align 200256 388 66 ; Align 228393 410 12 ; Align 236060 414 21 ; Align 244512 421 54 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000144_2.0 # Protein # Eukaryotic elongation factor (eEFSec) # Human (Homo sapiens) Target: gi|397462090|gb|JH733411.1|:subseq(110221,245320) Alligator mississippiensis unplaced genomic scaffold scaffold-3859, whole genome shotgun sequence Model: protein2genome:local Raw score: 101 Query range: 65 -> 374 Target range: 1138 -> 244186 66 : SerSerLeuProGluPheGln{Al} >>>> Target Intron 1 >>>> {a}A : 74 |||||||||||||||!::|||{!.} 10974 bp {!} SerSerLeuProGluTrpGln{Va}++ ++{l}T 1139 : AGTTCTCTTCCTGAGTGGCAG{GT}gt.........................ag{G}T : 12137 75 : laProGluAlaGluProGluProGlyGluProLeuLeuGlnValThrLeuValAspCy : 93 ! !.!. !:!! ! !|||.!!...||| !||| ..! !!!!:!! || yrIleHisTyrLysArgLeuProSerSerProGluLeuCysThrGlyPheMetMetCy 12138 : ATATTCATTACAAAAGGCTGCCAAGCTCTCCCGAGCTGTGCACTGGTTTCATGATGTG : 12194 94 : sPro >>>> Target Intron 2 >>>> GlyHisAlaSerLeuIleArgThrI : 103 |||| 11111 bp ||||||:!!.!!! !!!: !.!!. sPro++ ++GlyHisSerGlyCysMetAlaAlaP 12195 : CCCGgt.........................agGGTCATTCTGGTTGCATGGCAGCGT : 23335 104 : leIle{G} >>>> Target Intron 3 >>>> {ly}GlyAlaGlnIleIleAs : 111 !!:!!{|} 3987 bp {||} !|||.!.:!:! ! heLeu{G}++ ++{ly}***AlaAsnValThrAr 23336 : TCTTA{G}gt.........................ag{GT}TAGGCTAATGTGACTAG : 27346 112 : pLeuMetMetLeuValIleAspValThrLysGlyMetGlnThrGlnSerAla{G} > : 129 |||! ..!!!!|||||| !|||... !:!:.!. !:!!!:!|||{|} gLeuAsnGlnPheValIleThrGlnThrSerProLeuAsp***GluAsnAla{G}+- 27347 : GCTGAATCAGTTTGTAATCACACAGACATCCCCTCTTGACTGAGAAAATGCA{G}gc. : 27403 130 : >>> Target Intron 4 >>>> {lu}CysLeuValIleGlyGlnIle{A} >> : 137 14845 bp {||} !|||! !.!!..! :!!{|} ++{lu}LeuLeuAspPheAsnIleLeu{A}++ 27404 : ........................ag{AG}CTCCTTGACTTCAATATTTTA{G}gt.. : 42272 138 : >> Target Intron 5 >>>> {la}CysGlnLysLeuValValValLeuAsnLy : 147 17087 bp {||} ! ! ! !|||:!!..!:!!|||!: ++{la}IleThrAspTyrValIleThrIleAsnAr 42273 : .......................ag{CC}ATAACAGACTATGTTATTACTATCAATAG : 59386 148 : sIleAspLeuLeuProGluGly >>>> Target Intron 6 >>>> LysArg{ : 157 !! ! !||||||.!. ! 45456 bp !:!!:!{ gAsnMetSerLeuProHisLeu++ ++ArgLys{ 59387 : AAACATGAGCTTACCACACCTGgt.........................agAGAAAA{ : 104872 158 : G} >>>> Target Intron 7 >>>> {ln}AlaAlaIleAspLysMetThrLy : 165 .} 24447 bp {!.}!.! !:!:|||!!..!. !|| A}++ ++{sp}ValHisLeuAspAsnPheLeuLy 104873 : G}gt.........................ag{AT}GTGCACCTGGATAATTTTCTCAA : 129343 166 : sLysMetGlnLysThrLeu{G} >>>> Target Intron 8 >>>> {lu}Asn : 173 |:!!:!:! :!! !! !{|} 3393 bp {||}.!. sGluLeuProGlnAspArg{G}++ +-{lu}Gln 129344 : AGAGCTTCCCCAGGACCGA{G}gt.........................ac{AG}CAA : 132760 174 : ThrLysPheArgGlyAlaProIleIleProVal{A} >>>> Target Intron 9 : 185 !..!|||! !! !|||! !:!!!!:|||..!{!} 4901 bp LeuSerPheThrValAlaHisLeuMetProThr{G}-+ 132761 : CTCTCATTCACAGTAGCCCACTTAATGCCCACA{G}tt.................... : 132799 186 : >>>> {la}AlaLysProGlyGlyProGluAlaProGluThrGluAla{P} >>> : 199 {.!}::! !|||!.!..!||| !||| !||| !...|||{ } +-{ly}SerLeuProAlaSerProThrAlaGlyGluArgSerAla{A}++ 132800 : .....at{GG}AGCCTGCCTGCCTCTCCCACAGCCGGGGAGCGATCTGCT{G}gt... : 137742 200 : > Target Intron 10 >>>> {ro}GlnGlyIleProGluLeuIleGlu >>> : 208 2205 bp { !}! !.!:!!||||||! !!!:||| ++{sp}ProAlaLeuProGluGlnMetGlu++ 137743 : .......................ag{AT}CCTGCACTCCCTGAGCAAATGGAAgt... : 139973 209 : > Target Intron 11 >>>> LeuLeuThrSer >>>> Target Intron : 212 2136 bp ! !||| !..! 12583 bp ++ProLeuCysGlu++ 139974 : .......................agCCCCTGTGTGAGgt................... : 142121 213 : 12 >>>> GlnIleSerIleProThrArgAspProSerGlyProPheLeuMetSerV : 228 ... !|||:!!|||||| ! ! |||||| !|||.!.:!!:!: !! +-SerSerSerLeuProThrPheLysProSerGlnProLeuIleLeuProP 142122 : .......acTCCTCTTCACTCCCTACATTCAAACCCTCGCAGCCCCTGATTCTTCCTC : 154750 229 : alAspHisCysPheSerIleLysGlyGlnGlyThrValMetThrGlyThrIleLeuSe : 247 ! !:!!|||.!!!.!! !!.. !!..!.!!||| !! |||||||||! ! !!! roProAsnCysLeuLysThrSerArgMetSerThrLysArgThrGlyThrThrGluAr 154751 : CTCCCAACTGCCTCAAAACCAGCAGGATGAGCACTAAGAGAACTGGAACCACTGAGAG : 154807 248 : rGlySerIle{Se} >>>> Target Intron 13 >>>> {r}LeuGlyAspSe : 255 !! !||| !{..} 2897 bp {!}||| !:!!.. gGluSerGly{Gl}+- +-{u}LeuPheAsnGl 154808 : AGAGAGTGGA{GA}ga..........................at{G}TTGTTTAATGA : 157728 256 : rValGluIleProAlaLeuLysValValLysLysValLysSerMetGlnMet{Ph} : 273 !:!!! !:!:||| !:!!|||..!|||!!.!:!:!!!..!!!|||... { } uIleValValProTyrIleLysThrValAsnArgMetSerCysMetSerTyr{Se}++ 157729 : AATAGTGGTGCCATATATTAAAACAGTTAACAGGATGAGTTGCATGAGTTAT{AG}gt : 157784 274 : >>>> Target Intron 14 >>>> {e}HisMetProIleThrSerAlaMetGln : 282 61776 bp {!}||| ||| !!!!.!!:!!||| ++{r}HisProProProGlyCysThrLeuGln 157785 : ..........................ag{C}CACCCACCACCGGGCTGCACTCTGCAG : 219585 283 : GlyAspArgLeuGlyIleCysValThrGlnPheAspProLysLeuLeuGluArgGlyL : 302 |||! !! ! ! !|||!.! ! ! !!||| ! |||! !...! !|||! GlyGlyTrpSerGlnGlyCysAlaGlnGlyGlyTyrProTyrLeuHisSerProGlyS 219586 : GGTGGGTGGAGCCAGGGCTGTGCTCAGGGTGGTTATCCCTACCTACACAGCCCTGGCT : 219645 303 : euValCysAlaProGluSer{L} >>>> Target Intron 15 >>>> {eu}H : 310 ! !||| !!||||||..!{:} 17866 bp {!!}: erSerCysProProGluGln{M}++ ++{et}A 219646 : CCTCCTGCCCCCCAGAGCAA{A}gt..........................ag{TG}A : 237535 311 : isThrValHisAlaAlaLeuIleSerValGluLysIleProTyrPheArgGlyProLe : 329 !!|||!.! ! ! ! !!!|||! !:! |||! !!!.!:!||| !!:! snThrAlaAlaLysTrpLysGlnThrValAlaArgGlnProSerLeuLysGlyThrVa 237536 : ACACAGCAGCAAAGTGGAAACAGACAGTGGCCAGGCAGCCTTCTTTGAAAGGAACTGT : 237592 330 : uGlnThrLys{A} >>>> Target Intron 16 >>>> {la}LysPheHisIl : 337 ! !||||||{!} 3656 bp { !} ||| !|| lGlyThrLys{G}++ ++{lu}PhePhePheIl 237593 : AGGGACAAAA{G}gt..........................ag{AA}TTTTTTTTTAT : 241272 338 : eThrValGly{Hi} >>>> Target Intron 17 >>>> {s}GluThr{V} : 344 | ! !|||{||} 2297 bp {|}.!.|||{!} eLeuHisGly{Hi}++ ++{s}HisThr{A}++ 241273 : ACTTCATGGT{CA}gt..........................ag{T}CATACA{G}gt : 243591 345 : >>>> Target Intron 18 >>>> {al}MetGlyArgLeuMetPhePheSerPr : 353 504 bp {.!}! !!:! !:!:||||||.!!|| ++{la}SerThrGlnAlaLeuPhePheGlyPr 243592 : ..........................ag{CA}AGCACACAGGCCCTTTTTTTTGGTCC : 244121 354 : oAlaProAspAsnPheAspGlnGluProIleLeuAspSerPheAsnPheSerGlnGlu : 372 | !||| .!! !! !! !|||:!! ! ||| ! !:::!!!::... oLeuProThrValLeuIleLeuValProValLysThrSerProMetTrpAlaArgSer 244122 : ACTCCCCACAGTACTTATTCTGGTACCAGTAAAGACATCCCCAATGTGGGCCCGCTCC : 244178 373 : TyrLeu : 374 !::||| TrpLeu 244179 : TGGCTG : 244186 vulgar: SPP00000144_2.0 65 374 . gi|397462090|gb|JH733411.1|:subseq(110221,245320) 1138 244186 + 101 M 7 21 S 0 2 5 0 2 I 0 10970 3 0 2 S 1 1 M 21 63 5 0 2 I 0 11107 3 0 2 M 10 30 S 0 1 5 0 2 I 0 3983 3 0 2 S 1 2 M 23 69 S 0 1 5 0 2 I 0 14841 3 0 2 S 1 2 M 7 21 S 0 1 5 0 2 I 0 17083 3 0 2 S 1 2 M 17 51 5 0 2 I 0 45452 3 0 2 M 2 6 S 0 1 5 0 2 I 0 24443 3 0 2 S 1 2 M 14 42 S 0 1 5 0 2 I 0 3389 3 0 2 S 1 2 M 12 36 S 0 1 5 0 2 I 0 4897 3 0 2 S 1 2 M 13 39 S 0 1 5 0 2 I 0 2201 3 0 2 S 1 2 M 8 24 5 0 2 I 0 2132 3 0 2 M 4 12 5 0 2 I 0 12579 3 0 2 M 39 117 S 0 2 5 0 2 I 0 2893 3 0 2 S 1 1 M 21 63 S 0 2 5 0 2 I 0 61772 3 0 2 S 1 1 M 35 105 S 0 1 5 0 2 I 0 17862 3 0 2 S 1 2 M 23 69 S 0 1 5 0 2 I 0 3652 3 0 2 S 1 2 M 7 21 S 0 2 5 0 2 I 0 2293 3 0 2 S 1 1 M 2 6 S 0 1 5 0 2 I 0 500 3 0 2 S 1 2 M 30 90 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-25 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local gene 1139 244186 101 + . gene_id 2 ; sequence SPP00000144_2.0 ; gene_orientation + gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 1139 1161 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 1139 1161 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 1162 1163 . + . intron_id 1 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 1162 12135 . + . intron_id 1 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 12134 12135 . + . intron_id 0 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 12136 12199 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 12136 12199 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 12200 12201 . + . intron_id 2 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 12200 23310 . + . intron_id 2 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 23309 23310 . + . intron_id 1 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 23311 23341 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 23311 23341 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 23342 23343 . + . intron_id 3 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 23342 27328 . + . intron_id 3 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 27327 27328 . + . intron_id 2 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 27329 27400 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 27329 27400 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 27401 27402 . + . intron_id 4 ; splice_site "GC" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 27401 42245 . + . intron_id 4 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 42244 42245 . + . intron_id 3 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 42246 42269 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 42246 42269 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 42270 42271 . + . intron_id 5 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 42270 59356 . + . intron_id 5 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 59355 59356 . + . intron_id 4 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 59357 59409 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 59357 59409 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 59410 59411 . + . intron_id 6 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 59410 104865 . + . intron_id 6 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 104864 104865 . + . intron_id 5 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 104866 104872 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 104866 104872 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 104873 104874 . + . intron_id 7 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 104873 129319 . + . intron_id 7 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 129318 129319 . + . intron_id 6 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 129320 129364 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 129320 129364 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 129365 129366 . + . intron_id 8 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 129365 132757 . + . intron_id 8 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 132756 132757 . + . intron_id 7 ; splice_site "AC" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 132758 132796 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 132758 132796 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 132797 132798 . + . intron_id 9 ; splice_site "TT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 132797 137697 . + . intron_id 9 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 137696 137697 . + . intron_id 8 ; splice_site "AT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 137698 137739 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 137698 137739 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 137740 137741 . + . intron_id 10 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 137740 139944 . + . intron_id 10 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 139943 139944 . + . intron_id 9 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 139945 139970 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 139945 139970 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 139971 139972 . + . intron_id 11 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 139971 142106 . + . intron_id 11 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 142105 142106 . + . intron_id 10 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 142107 142118 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 142107 142118 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 142119 142120 . + . intron_id 12 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 142119 154701 . + . intron_id 12 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 154700 154701 . + . intron_id 11 ; splice_site "AC" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 154702 154820 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 154702 154820 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 154821 154822 . + . intron_id 13 ; splice_site "GA" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 154821 157717 . + . intron_id 13 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 157716 157717 . + . intron_id 12 ; splice_site "AT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 157718 157783 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 157718 157783 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 157784 157785 . + . intron_id 14 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 157784 219559 . + . intron_id 14 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 219558 219559 . + . intron_id 13 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 219560 219666 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 219560 219666 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 219667 219668 . + . intron_id 15 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 219667 237532 . + . intron_id 15 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 237531 237532 . + . intron_id 14 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 237533 237604 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 237533 237604 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 237605 237606 . + . intron_id 16 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 237605 241260 . + . intron_id 16 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 241259 241260 . + . intron_id 15 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 241261 241285 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 241261 241285 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 241286 241287 . + . intron_id 17 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 241286 243582 . + . intron_id 17 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 243581 243582 . + . intron_id 16 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 243583 243590 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 243583 243590 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 243591 243592 . + . intron_id 18 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 243591 244094 . + . intron_id 18 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 244093 244094 . + . intron_id 17 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 244095 244186 . + . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 244095 244186 . + . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local similarity 1139 244186 101 + . alignment_id 2 ; Query SPP00000144_2.0 ; Align 1139 66 21 ; Align 12137 74 63 ; Align 23311 95 30 ; Align 27331 106 69 ; Align 42248 130 21 ; Align 59359 138 51 ; Align 104866 155 6 ; Align 129322 158 42 ; Align 132760 173 36 ; Align 137700 186 39 ; Align 139947 200 24 ; Align 142107 208 12 ; Align 154702 212 117 ; Align 157719 252 63 ; Align 219561 274 105 ; Align 237535 310 69 ; Align 241263 334 21 ; Align 243584 342 6 ; Align 244097 345 90 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000144_2.0 # Protein # Eukaryotic elongation factor (eEFSec) # Human (Homo sapiens) Target: gi|397462090|gb|JH733411.1|:subseq(110221,245320) Alligator mississippiensis unplaced genomic scaffold scaffold-3859, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 2096 Query range: 12 -> 595 Target range: 245320 -> 49999 13 : LeuGlyHisIleAspSerGlyLysThrAlaLeuAlaArgAlaLeuSerThrThrAlaS : 32 |||||||||||||||||||||||||||||||||||||||||||||||||||.!!!.!| LeuGlyHisIleAspSerGlyLysThrAlaLeuAlaArgAlaLeuSerThrAlaGlyS 245320 : CTGGGCCACATCGACAGCGGCAAGACGGCGCTGGCCCGAGCGCTCAGCACCGCCGGCT : 245263 33 : erThrAlaAlaPheAspLysGlnProGlnSerArgGluArgGlyIleThrLeuAspLe : 51 |||||||||||||||||::: !||||||||||||! |||||||||||||||||||| erThrAlaAlaPheAspArgAlaProGlnSerArgAlaArgGlyIleThrLeuAspLe 245262 : CTACCGCCGCCTTCGACCGCGCGCCGCAGAGCCGCGCCCGCGGCATCACCCTGGACCT : 245206 52 : uGlyPheSerCysPheSerValProLeuProAlaArgLeuArgSerSerLeuProGlu : 70 |||||||||||||.!!!!!..! !!|||||| !!|||||| uGlyPheSerCysLeuCysThrAlaLeuProProArgLeu------------------ 245205 : GGGCTTCTCGTGCCTCTGCACCGCGCTGCCCCCGCGCCTC------------------ : 245164 71 : PheGlnAlaAlaProGluAlaGluProGluProGlyGluProLeuLeuGlnValThrL : 90 !.!|||! !!! |||! ||||||.!. !! !|||||| !!|||| ---------GlyProGlyProGlyProGlyProGlyHisGlyHisLeuGlnPheThrL 245163 : ---------GGCCCCGGCCCCGGCCCCGGCCCCGGCCACGGGCACCTGCAGTTCACGC : 245116 91 : euValAspCysProGlyHisAlaSerLeuIleArgThrIleIleGly{G} >>>> T : 106 |||||||||||||||||||||||||||||||||||||||||||||||{|} euValAspCysProGlyHisAlaSerLeuIleArgThrIleIleGly{G}++ 245115 : TGGTGGACTGCCCCGGCCACGCCTCCCTCATCCGGACCATCATCGGC{G}gt...... : 245065 107 : arget Intron 1 >>>> {ly}AlaGlnIleIleAspLeuMetMetLeuValIle : 117 53824 bp {||}||||||||||||||||||||||||||||||||| ++{ly}AlaGlnIleIleAspLeuMetMetLeuValIle 245064 : ...................ag{GT}GCCCAGATTATTGATTTGATGATGCTTGTAATA : 191211 118 : AspValThrLysGlyMetGlnThrGlnSerAlaGluCysLeuValIleGlyGlnIleA : 137 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| AspValThrLysGlyMetGlnThrGlnSerAlaGluCysLeuValIleGlyGlnIleA 191210 : GATGTGACAAAAGGGATGCAGACTCAGTCAGCAGAGTGCTTAGTGATTGGACAAATTG : 191151 138 : laCysGlnLysLeuValValValLeuAsnLysIleAspLeuLeuProGluGlyLysAr : 156 |||||||||||:!!|||||||||||||||||||||||||||||||||||||||||||| laCysGlnLysMetValValValLeuAsnLysIleAspLeuLeuProGluGlyLysAr 191150 : CTTGCCAGAAGATGGTAGTAGTTCTGAACAAAATAGATCTCCTTCCAGAGGGGAAGAG : 191094 157 : gGlnAlaAlaIleAspLysMetThrLysLysMetGlnLysThrLeuGluAsnThr{Ly : 175 |||||||||||||!!:|||||||||||||||||||||||||||||||||||||||{|| gGlnAlaAlaIleGluLysMetThrLysLysMetGlnLysThrLeuGluAsnThr{Ly 191093 : ACAGGCTGCCATTGAGAAGATGACCAAGAAAATGCAAAAGACCTTGGAAAATACA{AA : 191037 176 : } >>>> Target Intron 2 >>>> {s}PheArgGlyAlaProIleIleProV : 184 } 7355 bp {|}|||!.!|||..!||||||:!! !!| }++ ++{s}PheHisGlyCysProIleValAlaV 191036 : }gt.........................ag{G}TTCCATGGGTGTCCTATTGTTGCGG : 183655 185 : alAlaAlaLysProGlyGlyProGluAlaProGluThrGluAlaProGlnGlyIlePr : 203 ||||||||||||||||||||||||||||||||||||||! ! !|||! !|||:!! ! alAlaAlaLysProGlyGlyProGluAlaProGluThrValAsnProLeuGlyValSe 183654 : TGGCAGCTAAACCTGGTGGACCAGAAGCCCCAGAGACTGTGAATCCACTAGGTGTTTC : 183598 204 : oGluLeuIleGlu >>>> Target Intron 3 >>>> LeuLeuThrSerGlnI : 213 !|||||||||||| 3362 bp :!!|||! !|||||| rGluLeuIleGlu++ ++ValLeuLysSerGlnA 183597 : TGAATTAATTGAGgt.........................agGTCCTGAAGTCTCAGG : 180206 214 : leSerIleProThrArgAspProSerGlyProPheLeuMetSerValAspHisCysPh : 232 !! !:!:|||:!!|||||||||||||||||||||||||||:!!|||||||||||||| laTyrLeuProSerArgAspProSerGlyProPheLeuMetAlaValAspHisCysPh 180205 : CTTACCTGCCATCAAGAGATCCCTCTGGACCCTTCCTTATGGCAGTTGATCACTGTTT : 180149 233 : eSerIleLysGlyGlnGlyThrValMetThrGlyThrIleLeuSerGlySerIleSer : 251 |||||||||||||||||||||||||||||||||||||||||||!!!||||||:!!||| eSerIleLysGlyGlnGlyThrValMetThrGlyThrIleLeuThrGlySerValSer 180148 : CTCTATCAAGGGTCAAGGCACAGTGATGACAGGGACTATTCTTACTGGCTCTGTTAGC : 180092 252 : LeuGlyAspSerValGluIleProAlaLeuLys >>>> Target Intron 4 >> : 263 |||||||||!:!||||||||||||||||||||| 43037 bp LeuGlyAspAsnValGluIleProAlaLeuLys++ 180091 : CTAGGTGATAATGTGGAGATTCCCGCCCTGAAGgt....................... : 180054 264 : >> ValValLysLysValLysSerMetGlnMetPheHisMetProIleThrSerAla : 280 ||| !||||||||||||||||||||||||||||||! |||:!:|||! !||| ++ValSerLysLysValLysSerMetGlnMetPheHisThrProValThrTyrAla 180053 : ..agGTGTCAAAGAAAGTCAAGTCCATGCAGATGTTCCATACTCCCGTGACTTACGCC : 136968 281 : MetGlnGlyAspArgLeuGlyIleCysValThrGlnPheAspProLysLeuLeuGluA : 300 |||||||||||||||:!!|||||||||||||||||||||||||||||||||||||||| MetGlnGlyAspArgValGlyIleCysValThrGlnPheAspProLysLeuLeuGluA 136967 : ATGCAGGGGGACAGGGTAGGCATCTGTGTGACCCAGTTTGATCCCAAGCTGCTAGAAC : 136908 301 : rgGlyLeuValCysAlaProGluSerLeuHisThrValHisAlaAlaLeuIleSerVa : 319 ||||||||:!!|||.!!||||||||||||!!.|||:!!|||||||||:!!||||||:! rgGlyLeuIleCysThrProGluSerLeuGlnThrIleHisAlaAlaIleIleSerLe 136907 : GGGGTCTCATCTGTACCCCAGAATCTCTCCAGACCATACATGCTGCCATTATCTCCCT : 136851 320 : lGluLysIleProTyrPheArgGlyProLeuGlnThrLysAlaLysPheHisIleThr : 338 !:!!||||||! !|||||||||||| !!|||||||||||||||||||||||||||||| uLysLysIleGlnTyrPheArgGlyAlaLeuGlnThrLysAlaLysPheHisIleThr 136850 : GAAGAAAATTCAGTATTTTCGAGGTGCGCTGCAGACCAAAGCCAAGTTTCATATCACA : 136794 339 : ValGlyHisGluThrValMetGlyArgLeuMetPhePheSerProAlaProAspAsnP : 358 |||||||||||||||||||||||||||:!!|||||||||.!!|||||||||! !:!!| ValGlyHisGluThrValMetGlyArgValMetPhePheGlyProAlaProAlaAspP 136793 : GTTGGTCACGAAACAGTTATGGGACGAGTAATGTTCTTCGGTCCAGCCCCTGCTGACT : 136734 359 : heAspGlnGluProIleLeuAspSerPheAsnPheSerGlnGluTyrLeuPheGlnGl : 377 ||:!!:!!||||||:!: !:!!:!!|||:!!|||..!::!!!:||||||!:!||||| heAsnLysGluProLeuAspAsnAlaPheAspPheGluArgAspTyrLeuTyrGlnGl 136733 : TCAACAAAGAGCCCCTGGACAATGCCTTTGATTTTGAAAGAGATTACCTTTATCAAGA : 136677 378 : uGlnTyrLeuSerLysAspLeuThrProAlaValThrAspAsnAspGluAlaAspLys : 396 |:!!||||||||||||||| :!! !||| ! !!!:||| ! !!: !|||:!! uGluTyrLeuSerLysAsp---SerLysAlaSerAspGluAsnLysAspAsnAspGln 136676 : AGAATATTTGTCCAAGGAT---TCGAAGGCTTCTGATGAAAACAAGGATAATGACCAG : 136623 397 : LysAlaGlyGlnAlaThrGluGlyHisCysProArgGlnGlnTrpAlaLeuValGluP : 416 ! !!.!! !||| !|||!:!|||||||||||||||:!!|||| ThrGlyGluGln---------------LeuProLysGlnGlnTrpAlaLeuLeuGluP 136622 : ACAGGAGAGCAG---------------CTCCCGAAGCAACAGTGGGCCTTACTGGAGT : 136578 417 : heGluLysProValThrCysProArgLeuCysLeuValIleGlySerArgLeuAspAl : 435 |||||||||||||||||||||||||||||||||||||||||||||||!:!||||||.! heGluLysProValThrCysProArgLeuCysLeuValIleGlySerLysLeuAspTh 136577 : TTGAAAAACCAGTCACTTGTCCTCGACTCTGTCTTGTAATTGGGTCAAAACTGGATAC : 136521 436 : aAspIleHisThrAsnThrCysArgLeuAlaPheHisGlyIleLeuLeuHisGlyLeu : 454 !|||||||||.!!|||||||||||||||||||||||||||:!!||||||!!.|||||| rAspIleHisAlaAsnThrCysArgLeuAlaPheHisGlyValLeuLeuGlnGlyLeu 136520 : TGATATTCATGCAAACACCTGCAGGTTGGCATTCCATGGGGTATTGCTTCAAGGCCTA : 136464 455 : GluAspArgAsnTyrAlaAspSerPheLeuProArgLeuLysValTyrLysLeuLysH : 474 ||||||!:!||||||.!!!!:!!!|||||||||!:!|||||||||||||||||||||| GluAspLysAsnTyrThrGluThrPheLeuProLysLeuLysValTyrLysLeuLysH 136463 : GAAGATAAAAACTATACAGAGACTTTCCTTCCAAAGCTGAAAGTGTACAAACTGAAGC : 136404 475 : isLysHisGlyLeuValGluArg >>>> Target Intron 5 >>>> AlaMet : 483 |||||.!.|||! !||||||||| 20117 bp !.!!!: isLysGluGlyGlnValGluArg++ ++ValIle 136403 : ACAAAGAAGGTCAAGTGGAAAGGgt.........................agGTGATA : 116260 484 : AspAspTyrSerValIleGlyArgSerLeuPheLysLysGluThrAsnIleGlnLeuP : 503 ||||||||||||||||||||||||||||||||||||||||||||||||||||||:!!| AspAspTyrSerValIleGlyArgSerLeuPheLysLysGluThrAsnIleGlnIleP 116259 : GACGATTACAGCGTGATCGGTCGTTCGCTGTTTAAAAAGGAAACCAACATCCAGATTT : 116200 504 : heValGlyLeuLysValHisLeuSerThrGlyGluLeuGlyIleIleAspSerAlaPh : 522 ||||||||||||||||| ! ||||||||||||||| !|||||||||!!:.!!!.!|| heValGlyLeuLysValLysLeuSerThrGlyGluAspGlyIleIleGluGlyGlyPh 116199 : TTGTGGGGCTGAAAGTCAAGCTATCTACAGGAGAGGATGGCATAATAGAAGGTGGCTT : 116143 523 : eGlyGlnSerGlyLysPheLysIleHisIlePro{G} >>>> Target Intron : 534 |||||||||||||||||||||||||!..||||||{!} 65925 bp eGlyGlnSerGlyLysPheLysIleArgIlePro{A}++ 116142 : TGGGCAGAGTGGTAAATTTAAAATCCGAATCCCA{G}gt................... : 116104 535 : 6 >>>> {ly}GlyLeuSerProGluSerLysLysIleLeuThrProAlaLeuLysL : 550 { !}||||||!.!|||!!:!!!||| !:!!|||||| !|||! !|||| ++{sp}GlyLeuLysProAspThrLysAlaLeuLeuThrValAlaSerLysL 116103 : ......ag{AT}GGGCTCAAGCCAGACACAAAGGCACTTCTGACTGTTGCCTCTAAGA : 50134 551 : ysArgAlaArgAlaGlyArgGlyGluAlaThrArgGlnGluGluSerAlaGluArgSe : 569 ||!:!:!!|||||||||!:!|||!!:|||.!!!:!:!!!!:!!:|||.!! !:!:! ysLysSerArgAlaGlyLysGlyAspAlaAlaLysGluAspAspSerThr---LysAl 50133 : AGAAATCCAGAGCAGGGAAAGGGGATGCAGCCAAAGAGGATGACAGCACC---AAAGC : 50080 570 : rGluProSerGlnHisValValLeuSerLeuThrPheLysArgTyrValPheAspThr : 588 !||| !!:!!|||! !|||!.!:!!|||||| !|||||||||||||||||||||||| aGluSerAlaGlnProValAlaIleSerLeuLeuPheLysArgTyrValPheAspThr 50079 : TGAGTCAGCCCAGCCTGTTGCCATCAGCCTTCTCTTTAAAAGATACGTCTTTGACACG : 50023 589 : HisLysArgMetValGlnSer : 595 !!.|||!:!|||:!!|||||| GlnLysLysMetIleGlnSer 50022 : CAGAAGAAAATGATTCAGTCT : 50000 vulgar: SPP00000144_2.0 12 595 . gi|397462090|gb|JH733411.1|:subseq(110221,245320) 245320 49999 - 2096 M 52 156 G 9 0 M 32 96 S 0 1 5 0 2 I 0 53820 3 0 2 S 1 2 M 68 204 S 0 2 5 0 2 I 0 7351 3 0 2 S 1 1 M 32 96 5 0 2 I 0 3358 3 0 2 M 55 165 5 0 2 I 0 43033 3 0 2 M 121 363 G 1 0 M 16 48 G 5 0 M 76 228 5 0 2 I 0 20113 3 0 2 M 52 156 S 0 1 5 0 2 I 0 65921 3 0 2 S 1 2 M 32 96 G 1 0 M 28 84 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-25 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local gene 50000 245320 2096 - . gene_id 1 ; sequence SPP00000144_2.0 ; gene_orientation + gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 245068 245320 . - . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 245068 245320 . - . insertions 0 ; deletions 9 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 245066 245067 . - . intron_id 1 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 191244 245067 . - . intron_id 1 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 191244 191245 . - . intron_id 0 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 191036 191243 . - . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 191036 191243 . - . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 191034 191035 . - . intron_id 2 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 183681 191035 . - . intron_id 2 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 183681 183682 . - . intron_id 1 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 183584 183680 . - . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 183584 183680 . - . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 183582 183583 . - . intron_id 3 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 180222 183583 . - . intron_id 3 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 180222 180223 . - . intron_id 2 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 180057 180221 . - . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 180057 180221 . - . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 180055 180056 . - . intron_id 4 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 137020 180056 . - . intron_id 4 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 137020 137021 . - . intron_id 3 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 136381 137019 . - . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 136381 137019 . - . insertions 0 ; deletions 6 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 136379 136380 . - . intron_id 5 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 116264 136380 . - . intron_id 5 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 116264 116265 . - . intron_id 4 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 116107 116263 . - . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 116107 116263 . - . insertions 0 ; deletions 0 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice5 116105 116106 . - . intron_id 6 ; splice_site "GT" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local intron 50182 116106 . - . intron_id 6 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local splice3 50182 50183 . - . intron_id 5 ; splice_site "AG" gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local cds 50000 50181 . - . gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local exon 50000 50181 . - . insertions 0 ; deletions 1 gi|397462090|gb|JH733411.1|:subseq(110221,245320) exonerate:protein2genome:local similarity 50000 245320 2096 - . alignment_id 1 ; Query SPP00000144_2.0 ; Align 245321 13 156 ; Align 245165 74 96 ; Align 191242 107 204 ; Align 183680 176 96 ; Align 180222 208 165 ; Align 137020 263 363 ; Align 136657 385 48 ; Align 136609 406 228 ; Align 116264 482 156 ; Align 50180 535 96 ; Align 50084 568 84 # --- END OF GFF DUMP --- # -- completed exonerate analysis