TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000144_2.0 # Protein # Eukaryotic elongation factor
(eEFSec) # Human (Homo sapiens)
(596 letters)
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g
enome.fa
14,644 sequences; 2,174,243,242 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|397462090|gb|JH733411.1| Alligator mississippiensis unplaced ... 328 4e-88
gi|397461058|gb|JH734443.1| Alligator mississippiensis unplaced ... 44 0.017
gi|397460101|gb|JH735400.1| Alligator mississippiensis unplaced ... 42 0.086
gi|397460728|gb|JH734773.1| Alligator mississippiensis unplaced ... 42 0.086
gi|397461927|gb|JH733574.1| Alligator mississippiensis unplaced ... 39 0.95
gi|397457766|gb|JH737735.1| Alligator mississippiensis unplaced ... 36 6.1
>gi|397462090|gb|JH733411.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-3859, whole genome shotgun sequence
Length = 1675264
Score = 328 bits (842), Expect = 4e-88
Identities = 156/220 (70%), Positives = 188/220 (85%)
Frame = -1
Query: 262 KVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCAPESLHTVHAALISVEK 321
+V KKVKSMQMFH P+T AMQGDR+GICVTQFDPKLLERGL+C PESL T+HAA+IS++K
Sbjct: 247243 QVSKKVKSMQMFHTPVTYAMQGDRVGICVTQFDPKLLERGLICTPESLQTIHAAIISLKK 247064
Query: 322 IPYFRGPLQTKAKFHITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLS 381
I YFRG LQTKAKFHITVGHETVMGR+MFF PAP +F++EP+ ++F+F ++YL+QE+YLS
Sbjct: 247063 IQYFRGALQTKAKFHITVGHETVMGRVMFFGPAPADFNKEPLDNAFDFERDYLYQEEYLS 246884
Query: 382 KDLTPAVTDNDEADKKAGQATEGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADIHTNT 441
KD + A +N + D+ Q P+QQWAL+EFEKPVTCPRLCLVIGS+LD DIH NT
Sbjct: 246883 KD-SKASDENKDNDQTGEQ-----LPKQQWALLEFEKPVTCPRLCLVIGSKLDTDIHANT 246722
Query: 442 CRLAFHGILLHGLEDRNYADSFLPRLKVYKLKHKHGLVER 481
CRLAFHG+LL GLED+NY ++FLP+LKVYKLKHK G VER
Sbjct: 246721 CRLAFHGVLLQGLEDKNYTETFLPKLKVYKLKHKEGQVER 246602
Score = 134 bits (337), Expect = 1e-29
Identities = 68/70 (97%), Positives = 70/70 (100%)
Frame = -1
Query: 106 GAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDKMTK 165
GAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQK+VVVLNKIDLLPEGKRQAAI+KMTK
Sbjct: 301465 GAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKMVVVLNKIDLLPEGKRQAAIEKMTK 301286
Query: 166 KMQKTLENTK 175
KMQKTLENTK
Sbjct: 301285 KMQKTLENTK 301256
Score = 99.8 bits (247), Expect = 3e-19
Identities = 46/59 (77%), Positives = 55/59 (93%)
Frame = -2
Query: 205 LIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKV 263
+ ++L SQ +P+RDPSGPFLM+VDHCFSIKGQGTVMTGTIL+GS+SLGD+VEIPALKV
Sbjct: 290451 VFQVLKSQAYLPSRDPSGPFLMAVDHCFSIKGQGTVMTGTILTGSVSLGDNVEIPALKV 290275
Score = 91.3 bits (225), Expect = 1e-16
Identities = 45/61 (73%), Positives = 50/61 (81%)
Frame = -3
Query: 474 HKHGLVERAMDDYSVIGRSLFKKETNIQLFVGLKVHLSTGELGIIDSAFGQSGKFKIHIP 533
H + + +DDYSVIGRSLFKKETNIQ+FVGLKV LSTGE GII+ FGQSGKFKI IP
Sbjct: 226508 HMCAFLLQVIDDYSVIGRSLFKKETNIQIFVGLKVKLSTGEDGIIEGGFGQSGKFKIRIP 226329
Query: 534 G 534
G
Sbjct: 226328 G 226326
Score = 82.8 bits (203), Expect = 4e-14
Identities = 48/94 (51%), Positives = 49/94 (52%)
Frame = -3
Query: 13 LGHIDSGKXXXXXXXXXXXXXXXFDKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXX 72
LGHIDSGK FD+ PQSR RGITLDLGFSC LP RL
Sbjct: 355541 LGHIDSGKTALARALSTAGSTAAFDRAPQSRARGITLDLGFSCLCTALPPRL-------- 355386
Query: 73 XXXXXXXXXXXXXXQVTLVDCPGHASLIRTIIGG 106
Q TLVDCPGHASLIRTIIGG
Sbjct: 355385 -GPGPGPGPGHGHLQFTLVDCPGHASLIRTIIGG 355287
Score = 69.7 bits (169), Expect = 4e-10
Identities = 32/61 (52%), Positives = 49/61 (80%)
Frame = -3
Query: 535 GLSPESKKILTPALKKRARAGRGEATRQEESAERSEPSQHVVLSLTFKRYVFDTHKRMVQ 594
GL P++K +LT A KK++RAG+G+A ++++S ++E +Q V +SL FKRYVFDT K+M+Q
Sbjct: 160400 GLKPDTKALLTVASKKKSRAGKGDAAKEDDST-KAESAQPVAISLLFKRYVFDTQKKMIQ 160224
Query: 595 S 595
S
Sbjct: 160223 S 160221
>gi|397461058|gb|JH734443.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-5558, whole genome shotgun sequence
Length = 376683
Score = 44.3 bits (103), Expect = 0.017
Identities = 23/69 (33%), Positives = 36/69 (52%)
Frame = -2
Query: 236 GQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDP 295
G GTV G + +G + G V + + +VKS++M H ++ A+ GD +G V
Sbjct: 49412 GIGTVPVGRVETGILRPGMVVTFAPVNITTEVKSVEMHHEALSEALPGDNVGFNVKNVSV 49233
Query: 296 KLLERGLVC 304
K + RG VC
Sbjct: 49232 KDIRRGNVC 49206
>gi|397460101|gb|JH735400.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-7105, whole genome shotgun sequence
Length = 113958
Score = 42.0 bits (97), Expect = 0.086
Identities = 24/72 (33%), Positives = 36/72 (50%)
Frame = +3
Query: 232 FSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRLGICVT 291
F G GTV G + +G + G V + V +VKS++M H ++ A+ GD +G V
Sbjct: 94446 FFFLGIGTVPVGRVETGILKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVK 94625
Query: 292 QFDPKLLERGLV 303
K + RG V
Sbjct: 94626 NVSVKDVRRGNV 94661
>gi|397460728|gb|JH734773.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-6101, whole genome shotgun sequence
Length = 1314287
Score = 42.0 bits (97), Expect = 0.086
Identities = 23/68 (33%), Positives = 34/68 (50%)
Frame = +1
Query: 236 GQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDP 295
G GTV G + +G++ G V V +VKS++M H + A+ GD +G V
Sbjct: 849010 GIGTVPVGRVETGTLKAGMVVTFAPSNVTTEVKSVEMHHEALAEALPGDNVGFNVKNVSV 849189
Query: 296 KLLERGLV 303
K + RG V
Sbjct: 849190 KDIRRGNV 849213
>gi|397461927|gb|JH733574.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-4106, whole genome shotgun sequence
Length = 586228
Score = 38.5 bits (88), Expect = 0.95
Identities = 23/68 (33%), Positives = 33/68 (48%)
Frame = +3
Query: 236 GQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDP 295
G GTV G I +G + G +V + +V+S++M H P+ A G +G V
Sbjct: 160224 GVGTVPVGKIETGILKPGMTVSFAPTNLSAEVRSIEMHHEPLEVAFPGYNIGFNVKNIAV 160403
Query: 296 KLLERGLV 303
K L RG V
Sbjct: 160404 KNLRRGHV 160427
>gi|397457766|gb|JH737735.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-11001, whole genome shotgun sequence
Length = 338457
Score = 35.8 bits (81), Expect = 6.1
Identities = 14/30 (46%), Positives = 22/30 (73%)
Frame = +3
Query: 88 VTLVDCPGHASLIRTIIGGAQIIDLMMLVI 117
V+ VDCPGH L+ T++ GA ++D +L+I
Sbjct: 267180 VSFVDCPGHDILMATMLNGAAVMDAALLLI 267269
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis
/genome.fa
Posted date: Oct 18, 2013 5:53 PM
Number of letters in database: 2,174,243,242
Number of sequences in database: 14,644
Lambda K H
0.321 0.137 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,379,146,557
Number of Sequences: 14644
Number of extensions: 20573001
Number of successful extensions: 77152
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 440
Number of HSP's successfully gapped in prelim test: 327
Number of HSP's that attempted gapping in prelim test: 73591
Number of HSP's gapped (non-prelim): 10352
length of query: 596
length of database: 724,747,747
effective HSP length: 136
effective length of query: 460
effective length of database: 722,756,163
effective search space: 332467834980
effective search space used: 332467834980
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 79 (35.0 bits)