TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000016_2.0 # Protein # Iodothyronine deiodinase 3 (DI3) # Human (Homo sapiens) (278 letters) Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g enome.fa 14,644 sequences; 2,174,243,242 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|397456552|gb|JH738948.1| Alligator mississippiensis unplaced ... 333 6e-90 gi|397463745|gb|JH731756.1| Alligator mississippiensis unplaced ... 142 2e-32 gi|397464218|gb|JH731283.1| Alligator mississippiensis unplaced ... 62 3e-08 gi|397460703|gb|JH734798.1| Alligator mississippiensis unplaced ... 38 0.40 gi|397456056|gb|JH739444.1| Alligator mississippiensis unplaced ... 37 0.90 gi|397459189|gb|JH736312.1| Alligator mississippiensis unplaced ... 35 3.4 gi|397462156|gb|JH733345.1| Alligator mississippiensis unplaced ... 35 4.5 gi|397457120|gb|JH738380.1| Alligator mississippiensis unplaced ... 34 5.8 gi|397458166|gb|JH737335.1| Alligator mississippiensis unplaced ... 34 5.8 gi|397460541|gb|JH734960.1| Alligator mississippiensis unplaced ... 34 5.8 gi|397457484|gb|JH738017.1| Alligator mississippiensis unplaced ... 33 9.9 >gi|397456552|gb|JH738948.1| Alligator mississippiensis unplaced genomic scaffold scaffold-13070, whole genome shotgun sequence Length = 167594 Score = 333 bits (853), Expect = 6e-90 Identities = 159/259 (61%), Positives = 198/259 (76%) Frame = +2 Query: 14 AQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLGRRRRGQPEPEVELNSEGEEVPPDDPP 73 AQ A+CL+LFPRFL +A MLWLLDFLCIRK L G P + GE PPDDPP Sbjct: 61028 AQAAACLLLFPRFLLSAAMLWLLDFLCIRKKVLA----GGPAAAAAAAAAGEP-PPDDPP 61192 Query: 74 ICVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVLPDGFQSQHILDYAQGNR 133 +CVSD NRL T SLKAVWHG KLD FK AH G APN EV+ DG + +LD+A+G R Sbjct: 61193 LCVSDSNRLFTRESLKAVWHGHKLDLFKAAHVGARAPNPEVIRLDGQKRLRLLDFARGKR 61372 Query: 134 PLVLNFGSCTXPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYIIPQH 193 PLVLNFGSCT PPFMAR+ AFQRL + DFL++YIEEAHPSDGWV++D+ Y IP+H Sbjct: 61373 PLVLNFGSCT*PPFMARLRAFQRLAAHFLDIADFLLVYIEEAHPSDGWVSSDAAYKIPKH 61552 Query: 194 RSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDG 253 + L+DR+ AA+++++GAPGC L +DTM N+SS+AYGAYFERLY++Q +MYQGGRGP+G Sbjct: 61553 QCLQDRLRAAQLMREGAPGCPLAVDTMDNASSAAYGAYFERLYIVQEERVMYQGGRGPEG 61732 Query: 254 YQVSELRTWLERYDEQLHG 272 Y++SELR+WL++Y +L G Sbjct: 61733 YKISELRSWLDQYKTRLRG 61789 >gi|397463745|gb|JH731756.1| Alligator mississippiensis unplaced genomic scaffold scaffold-1225, whole genome shotgun sequence Length = 1234326 Score = 142 bits (358), Expect = 2e-32 Identities = 77/189 (40%), Positives = 111/189 (58%), Gaps = 22/189 (11%) Frame = -1 Query: 99 FFKQAHEGGPAPNSEVV-LPDGFQSQ---------------HILDYAQGNRPLVLNFGSC 142 F Q GG APNS V+ + +G H+LD+A RPLV+NFGS Sbjct: 1182699 FCHQVKLGGEAPNSRVIHITNGISGGSTRCKNVGGKLGSKCHLLDFANSERPLVVNFGSA 1182520 Query: 143 TXPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVT---TDSPYIIPQHRSLEDR 199 T PPF +++SAF +LV ++ DFL++YI+EAHPSDGW + S + + +H++ EDR Sbjct: 1182519 T*PPFTSQLSAFSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKHKNQEDR 1182340 Query: 200 VSAARVLQQG---APGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGYQV 256 +AA L Q P C +V D M N+++ AYG FER+ ++Q I Y GG+GP Y + Sbjct: 1182339 CAAAHQLLQRFSLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNL 1182160 Query: 257 SELRTWLER 265 E++ WLE+ Sbjct: 1182159 QEVQLWLEQ 1182133 >gi|397464218|gb|JH731283.1| Alligator mississippiensis unplaced genomic scaffold scaffold-461, whole genome shotgun sequence Length = 1270512 Score = 62.0 bits (149), Expect = 3e-08 Identities = 28/46 (60%), Positives = 33/46 (71%) Frame = -2 Query: 131 GNRPLVLNFGSCTXPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAH 176 GNRPLVLNFGSCT P F+ + F +LV + DFL+IYIEEAH Sbjct: 1131332 GNRPLVLNFGSCT*PSFLLKFDEFNKLVEDFNPVADFLLIYIEEAH 1131195 Score = 60.1 bits (144), Expect = 1e-07 Identities = 31/68 (45%), Positives = 44/68 (64%) Frame = -3 Query: 179 DGWVTTDSPYIIPQHRSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVI 238 DGW ++ +I H++LEDR AAR L + P C +V+DTM N SSS Y A ERLY++ Sbjct: 1128880 DGWAFKNN-IVIKNHQNLEDRKMAARFLLKKNPLCPVVVDTMENLSSSKYAALPERLYLL 1128704 Query: 239 QSGTIMYQ 246 Q ++Y+ Sbjct: 1128703 QGRKVVYK 1128680 >gi|397460703|gb|JH734798.1| Alligator mississippiensis unplaced genomic scaffold scaffold-6149, whole genome shotgun sequence Length = 339794 Score = 38.1 bits (87), Expect = 0.40 Identities = 22/48 (45%), Positives = 26/48 (54%) Frame = +3 Query: 35 LLDFLCIRKHFLGRRRRGQPEPEVELNSEGEEVPPDDPPICVSDDNRL 82 LL L R GRR+R Q E E E E +E P +DPP SDD R+ Sbjct: 199509 LLPLLPCRSRGRGRRQRVQEEGEGESQEEADEDPLEDPPPPPSDDTRV 199652 >gi|397456056|gb|JH739444.1| Alligator mississippiensis unplaced genomic scaffold scaffold-13912, whole genome shotgun sequence Length = 138699 Score = 37.0 bits (84), Expect = 0.90 Identities = 30/100 (30%), Positives = 47/100 (47%), Gaps = 17/100 (17%) Frame = +3 Query: 170 IYIEEAHPSDGWVTTDSPYIIPQHRSLED--------RVSAARVLQQGAPGCALVLDTMA 221 I + E H S+GW T P++ +HR+++ RV A + AP A+V + + Sbjct: 19368 IILSEVH-SEGWRKTKGPFLSSRHRAVQSFHTSACHARVDAESAEVRPAPSTAIVSPSSS 19544 Query: 222 NSSSSAYGAYF-------ERLYVI--QSGTIMYQGGRGPD 252 + S YF ERL+ I + G+ QGGRG + Sbjct: 19545 TTLKSRSSLYFLRFTWRPERLFCIPNKKGS---QGGRGAE 19655 >gi|397459189|gb|JH736312.1| Alligator mississippiensis unplaced genomic scaffold scaffold-8616, whole genome shotgun sequence Length = 658311 Score = 35.0 bits (79), Expect = 3.4 Identities = 19/47 (40%), Positives = 23/47 (48%), Gaps = 3/47 (6%) Frame = +1 Query: 49 RRRGQPEPEVELNSEGEEVPP---DDPPICVSDDNRLCTLASLKAVW 92 R+ G P P E + G PP +PPIC+ DD RL LA W Sbjct: 94567 RQIGGPHPR-EQSGTGPTAPPRGAGEPPICLPDDRRLVLLAGCSLCW 94704 >gi|397462156|gb|JH733345.1| Alligator mississippiensis unplaced genomic scaffold scaffold-3762, whole genome shotgun sequence Length = 1948850 Score = 34.7 bits (78), Expect = 4.5 Identities = 28/98 (28%), Positives = 48/98 (48%), Gaps = 11/98 (11%) Frame = -1 Query: 187 PYIIPQHRSLEDRVSAARVLQQGAP----GCALVLDTMANSSSSAYGAYFERLY------ 236 P+ + HR L V+A ++ + AP G LV D + + A+F ++ Sbjct: 64553 PHAVRPHR-LPWPVAARKIRESMAP*LNGGGDLVTDDVGKAE--VLNAFFTSVFTGKVSY 64383 Query: 237 -VIQSGTIMYQGGRGPDGYQVSELRTWLERYDEQLHGA 273 + G++++ GG P Q ++R + +R DEQLHGA Sbjct: 64382 QITAPGSMIWGGGEQPTVLQ-EQIRNYFKRLDEQLHGA 64272 >gi|397457120|gb|JH738380.1| Alligator mississippiensis unplaced genomic scaffold scaffold-12079, whole genome shotgun sequence Length = 31063 Score = 34.3 bits (77), Expect = 5.8 Identities = 21/75 (28%), Positives = 31/75 (41%) Frame = +1 Query: 30 AFMLWLLDFLCIRKHFLGRRRRGQPEPEVELNSEGEEVPPDDPPICVSDDNRLCTLASLK 89 +F LW DF K+ L +P ++ + VP PP C+SD +R CT + + Sbjct: 28660 SFTLWRCDF----KYSLSLTNWAKP-----VSFQDARVPATQPPECISDTSRSCTFSICR 28812 Query: 90 AVWHGQKLDFFKQAH 104 H L H Sbjct: 28813 CALHPSVLKRLDPGH 28857 >gi|397458166|gb|JH737335.1| Alligator mississippiensis unplaced genomic scaffold scaffold-10334, whole genome shotgun sequence Length = 131203 Score = 34.3 bits (77), Expect = 5.8 Identities = 39/122 (31%), Positives = 53/122 (43%), Gaps = 6/122 (4%) Frame = +3 Query: 31 FMLWLLDFLCIRKHFLGRRRRGQPEPEVELNSEGEEVPPDDPPICVSDDNRLCTLASLKA 90 F L FLC+ + FL +R G+P +V + GE RL L +L Sbjct: 35499 FFFLLHRFLCLCRQFLAKRGVGEP-GKVPGHKAGE---------------RLHLLGALIP 35630 Query: 91 VW--HGQKLDFFK---QAHEGGPAPNSEVVLPDGFQSQ-HILDYAQGNRPLVLNFGSCTX 144 + G++L FFK A GGP P + LP G +Q +L A+ P L GS T Sbjct: 35631 MXLPAGRQLSFFKSPGDASPGGPGPTAPRSLPWGAGAQTSLLGPARRAWPCSLGTGSPTP 35810 Query: 145 PP 146 P Sbjct: 35811 LP 35816 >gi|397460541|gb|JH734960.1| Alligator mississippiensis unplaced genomic scaffold scaffold-6410, whole genome shotgun sequence Length = 109535 Score = 34.3 bits (77), Expect = 5.8 Identities = 17/49 (34%), Positives = 22/49 (44%) Frame = +2 Query: 44 HFLGRRRRGQPEPEVELNSEGEEVPPDDPPICVSDDNRLCTLASLKAVW 92 H LG ++ P E L PP PP+C +CTL +L VW Sbjct: 64769 HILGTKQHPAPLDEFPL-------PPSSPPLCPPHQIAVCTLTALHLVW 64894 >gi|397457484|gb|JH738017.1| Alligator mississippiensis unplaced genomic scaffold scaffold-11477, whole genome shotgun sequence Length = 290835 Score = 33.5 bits (75), Expect = 9.9 Identities = 23/50 (46%), Positives = 27/50 (54%) Frame = +3 Query: 20 LVLFPRFLGTAFMLWLLDFLCIRKHFLGRRRRGQPEPEVELNSEGEEVPP 69 LVL P G L+LL+ LC H+LGR GQP E+EL S V P Sbjct: 137754 LVLCPGAKGAPRHLFLLERLC--SHWLGRIPPGQPCTELELPSMRRNVVP 137897 Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis /genome.fa Posted date: Oct 18, 2013 5:53 PM Number of letters in database: 2,174,243,242 Number of sequences in database: 14,644 Lambda K H 0.322 0.139 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 905,572,806 Number of Sequences: 14644 Number of extensions: 18029525 Number of successful extensions: 62864 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 742 Number of HSP's successfully gapped in prelim test: 514 Number of HSP's that attempted gapping in prelim test: 58249 Number of HSP's gapped (non-prelim): 13081 length of query: 278 length of database: 724,747,747 effective HSP length: 128 effective length of query: 150 effective length of database: 722,873,315 effective search space: 108430997250 effective search space used: 108430997250 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 75 (33.5 bits) qqqqqq