Command line: [exonerate -m p2g --showtargetgff -q ../Sel.human/DI2.fa -t gi|397463745|gb|JH731756.1|subseq.fa -E] Hostname: [ws101062] ** (process:3203): WARNING **: Exhaustively generating suboptimal alignments will be VERY SLOW C4 Alignment: ------------ Query: SPP00000011_2.0 # Protein # Iodothyronine deiodinase 2 (DI2) # Human (Homo sapiens) Target: gi|397463745|gb|JH731756.1|:subseq(1130000,100000) Alligator mississippiensis unplaced genomic scaffold scaffold-1225, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 1056 Query range: 0 -> 301 Target range: 64210 -> 52117 1 : MetGlyIleLeuSerValAspLeuLeuIleThrLeuGlnIleLeuProValPhePheSer : 20 ||||||:!:||||||||||||||||||||||||||||||||||||||||||||||||||| MetGlyLeuLeuSerValAspLeuLeuIleThrLeuGlnIleLeuProValPhePheSer 64210 : ATGGGTCTGTTAAGCGTGGATTTGCTGATCACGCTTCAGATCTTGCCAGTTTTTTTCTCC : 64153 21 : AsnCysLeuPheLeuAlaLeuTyrAspSerValIleLeuLeuLysHisValValLeuLeu : 40 ||||||||||||||||||||||||||||||||||||||||||||||||:!!||||||!!! AsnCysLeuPheLeuAlaLeuTyrAspSerValIleLeuLeuLysHisMetValLeuPhe 64152 : AATTGCCTCTTCCTTGCACTCTATGATTCTGTGATCCTCCTGAAGCACATGGTGCTGTTT : 64093 41 : LeuSerArgSerLysSerThrArgGlyGluTrpArgArgMetLeuThrSerGluGlyLeu : 60 ||||||||||||||||||.!!||||||||||||||||||||||||||||||||||||||| LeuSerArgSerLysSerAlaArgGlyGluTrpArgArgMetLeuThrSerGluGlyLeu 64092 : CTGAGTCGGTCTAAGTCTGCGCGTGGTGAGTGGCGAAGGATGCTGACCTCAGAGGGGCTG : 64033 61 : ArgCysValTrpLysSerPheLeuLeuAspAlaTyrLysGln >>>> Target Intr : 75 ||||||||||||!!.||||||||||||||||||||||||||| 2355 bp ArgCysValTrpAsnSerPheLeuLeuAspAlaTyrLysGln++ 64032 : CGTTGCGTCTGGAATAGCTTCCTCCTAGATGCTTACAAACAGgt................ : 63986 76 : on 1 >>>> LeuAsnCysProProSerGlyPheSerLysAsp >>>> Target In : 86 :!!..!! !||| !|||||| ! ..! !! 3036 ++ValGlyPheProLysSerGlyValGluLeuTyr++ 63985 : .........agGTAGGTTTTCCCAAGTCTGGAGTGGAACTTTATgt.............. : 61598 87 : tron 2 >>>> GlyHisIleLeuUnkLeuValTyrGluAlaTyrLysSerArgLeuLe : 101 bp ! :!!||| |||:!! ! ! !! !||| !!!!|||||||| ++HisSerValLeuProLeuLeuGluAlaGluTyrTrpThrArgLeuLe 61597 : ...........agCATTCAGTCCTGCCACTGTTAGAGGCAGAATACTGGACAAGACTGTT : 58519 102 : uValTyrSerHisLeu >>>> Target Intron 3 >>>> AspLeuTrpMet > : 111 ||||! !! !:!! 4634 bp !!:|||||| uVal***ProSerMet++ +-GluLeuTrpAsp++ 58518 : GGTCTGACCCAGTATGgt.........................atGAACTTTGGGATgt. : 53853 112 : >>> Target Intron 4 >>>> ValLysLeuGlyGluAspAlaProAsnSerSerV : 122 1168 bp ||||||||||||! !!!:||||||||||||!!!| ++ValLysLeuGlyGlyGluAlaProAsnSerArgV 53852 : ........................agGTGAAACTGGGTGGAGAAGCCCCAAACTCCAGAG : 52654 123 : alValHisValSerSerThrGluGlyGlyAspAsnSerGlyAsnGlyThrGlnGluLysI : 142 ||:!!|||:!!!!!!:! ! .!!||| ..!||| !!.. !!! !.!.! alIleHisIleThrAsnGlyIleSerGly---GlySerThrArgCysLysAsnValGlyG 52653 : TGATTCACATAACCAATGGCATCAGTGGT---GGCAGTACCAGATGCAAGAATGTTGGTG : 52597 143 : leAlaGluGlyAlaThrCysHisLeuLeuAspPheAlaSerProGluArgProLeuValV : 162 ! ! !|||::!! !|||||||||||||||||||||!:! !!|||||||||||||||| lyLysLeuGlySerLysCysHisLeuLeuAspPheAlaAsnSerGluArgProLeuValV 52596 : GAAAGTTGGGGAGCAAGTGTCATCTCTTGGATTTTGCCAACTCTGAGCGTCCCCTGGTGG : 52537 163 : alAsnPheGlySerAlaThrUnkProProPheThrSerGlnLeuProAlaPheArgLysL : 182 |||||||||||||||||||| ||||||||||||||||||||| !!||||||!!!|||| alAsnPheGlySerAlaThr***ProProPheThrSerGlnLeuSerAlaPheSerLysL 52536 : TCAACTTTGGTTCAGCTACCTGACCTCCATTCACGAGCCAGCTGTCAGCCTTCAGCAAGC : 52477 183 : euValGluGluPheSerSerValAlaAspPheLeuLeuValTyrIleAspGluAlaHisP : 202 |||||||||||||||||.!!|||||||||||||||||||||||||||||||||||||||| euValGluGluPheSerGlyValAlaAspPheLeuLeuValTyrIleAspGluAlaHisP 52476 : TGGTGGAGGAGTTTTCAGGTGTGGCCGACTTCCTGTTGGTCTACATTGACGAGGCTCACC : 52417 203 : roSerAspGlyTrpAlaIleProGlyAspSerSerLeuSerPheGluValLysLysHisG : 222 ||||||||||||||||| !|||||| !||| !!! !|||||||||||||||||||||: roSerAspGlyTrpAlaAlaProGlyIleSerProSerSerPheGluValLysLysHisL 52416 : CATCGGATGGTTGGGCTGCTCCTGGAATCTCGCCCTCTTCATTTGAGGTGAAGAAGCACA : 52357 223 : lnAsnGlnGluAspArgCysAlaAlaAlaGlnGlnLeuLeuGluArgPheSerLeuProP : 242 !!|||||||||||||||||||||||||||!!.|||||||||:!!|||||||||||||||| ysAsnGlnGluAspArgCysAlaAlaAlaHisGlnLeuLeuGlnArgPheSerLeuProP 52356 : AGAACCAGGAAGACCGATGTGCAGCTGCTCACCAGCTCCTGCAGAGGTTTTCCTTGCCCC : 52297 243 : roGlnCysArgValValAlaAspArgMetAspAsnAsnAlaAsnIleAlaTyrGlyValA : 262 ||||||||!:!|||||||||||| !!||||||||||||||||||:!!||||||||||||: roGlnCysGlnValValAlaAspCysMetAspAsnAsnAlaAsnValAlaTyrGlyValS 52296 : CCCAGTGCCAAGTAGTGGCTGACTGCATGGATAATAATGCCAATGTAGCCTATGGGGTCT : 52237 263 : laPheGluArgValCysIleValGlnArgGlnLysIleAlaTyrLeuGlyGlyLysGlyP : 282 !!|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| erPheGluArgValCysIleValGlnArgGlnLysIleAlaTyrLeuGlyGlyLysGlyP 52236 : CCTTTGAGCGCGTGTGTATCGTGCAGCGACAGAAAATTGCTTACCTGGGGGGCAAGGGCC : 52177 283 : roPheSerTyrAsnLeuGlnGluValArgHisTrpLeuGluLysAsnPheSerLysArg : 301 |||||! !||||||||||||||||||!:!! !|||||||||:!!|||! !||||||||| roPhePheTyrAsnLeuGlnGluValGlnLeuTrpLeuGluGlnAsnCysSerLysArg 52176 : CCTTTTTCTACAACCTCCAGGAAGTTCAGCTTTGGCTTGAACAAAACTGCAGCAAGAGA : 52118 vulgar: SPP00000011_2.0 0 301 . gi|397463745|gb|JH731756.1|:subseq(1130000,100000) 64210 52117 - 1056 M 74 222 5 0 2 I 0 2351 3 0 2 M 11 33 5 0 2 I 0 3032 3 0 2 M 21 63 5 0 2 I 0 4630 3 0 2 M 4 12 5 0 2 I 0 1164 3 0 2 M 21 63 G 1 0 M 169 507 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-15 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local gene 52118 64210 1056 - . gene_id 1 ; sequence SPP00000011_2.0 ; gene_orientation + gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local cds 63989 64210 . - . gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local exon 63989 64210 . - . insertions 0 ; deletions 0 gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local splice5 63987 63988 . - . intron_id 1 ; splice_site "GT" gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local intron 61634 63988 . - . intron_id 1 gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local splice3 61634 61635 . - . intron_id 0 ; splice_site "AG" gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local cds 61601 61633 . - . gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local exon 61601 61633 . - . insertions 0 ; deletions 0 gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local splice5 61599 61600 . - . intron_id 2 ; splice_site "GT" gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local intron 58565 61600 . - . intron_id 2 gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local splice3 58565 58566 . - . intron_id 1 ; splice_site "AG" gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local cds 58502 58564 . - . gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local exon 58502 58564 . - . insertions 0 ; deletions 0 gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local splice5 58500 58501 . - . intron_id 3 ; splice_site "GT" gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local intron 53868 58501 . - . intron_id 3 gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local splice3 53868 53869 . - . intron_id 2 ; splice_site "AT" gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local cds 53856 53867 . - . gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local exon 53856 53867 . - . insertions 0 ; deletions 0 gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local splice5 53854 53855 . - . intron_id 4 ; splice_site "GT" gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local intron 52688 53855 . - . intron_id 4 gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local splice3 52688 52689 . - . intron_id 3 ; splice_site "AG" gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local cds 52118 52687 . - . gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local exon 52118 52687 . - . insertions 0 ; deletions 1 gi|397463745|gb|JH731756.1|:subseq(1130000,100000) exonerate:protein2genome:local similarity 52118 64210 1056 - . alignment_id 1 ; Query SPP00000011_2.0 ; Align 64211 1 222 ; Align 61634 75 33 ; Align 58565 86 63 ; Align 53868 107 12 ; Align 52688 111 63 ; Align 52625 133 507 # --- END OF GFF DUMP --- # -- completed exonerate analysis