TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000011_2.0 # Protein # Iodothyronine deiodinase 2 (DI2) # Human (Homo sapiens) (309 letters) Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g enome.fa 14,644 sequences; 2,174,243,242 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|397463745|gb|JH731756.1| Alligator mississippiensis unplaced ... 289 9e-77 gi|397456552|gb|JH738948.1| Alligator mississippiensis unplaced ... 150 9e-35 gi|397464218|gb|JH731283.1| Alligator mississippiensis unplaced ... 61 7e-08 gi|397458870|gb|JH736631.1| Alligator mississippiensis unplaced ... 36 1.8 gi|397457284|gb|JH738216.1| Alligator mississippiensis unplaced ... 34 7.0 gi|397463755|gb|JH731746.1| Alligator mississippiensis unplaced ... 34 9.1 >gi|397463745|gb|JH731756.1| Alligator mississippiensis unplaced genomic scaffold scaffold-1225, whole genome shotgun sequence Length = 1234326 Score = 289 bits (740), Expect = 9e-77 Identities = 146/192 (76%), Positives = 161/192 (83%), Gaps = 1/192 (0%) Frame = -1 Query: 111 VKLGEDAPNSSVVHVSS-TEGGDNSGNGTQEKIAEGATCHLLDFASPERPLVVNFGSATX 169 VKLG +APNS V+H+++ GG K+ G+ CHLLDFA+ ERPLVVNFGSAT Sbjct: 1182687 VKLGGEAPNSRVIHITNGISGGSTRCKNVGGKL--GSKCHLLDFANSERPLVVNFGSAT* 1182514 Query: 170 PPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPSDGWAIPGDSSLSFEVKKHQNQEDRCA 229 PPFTSQL AF KLVEEFS VADFLLVYIDEAHPSDGWA PG S SFEVKKH+NQEDRCA Sbjct: 1182513 PPFTSQLSAFSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKHKNQEDRCA 1182334 Query: 230 AAQQLLERFSLPPQCRVVADRMDNNANIAYGVAFERVCIVQRQKIAYLGGKGPFSYNLQE 289 AA QLL+RFSLPPQC+VVAD MDNNAN+AYGV+FERVCIVQRQKIAYLGGKGPF YNLQE Sbjct: 1182333 AAHQLLQRFSLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQE 1182154 Query: 290 VRHWLEKNFSKR 301 V+ WLE+N SKR Sbjct: 1182153 VQLWLEQNCSKR 1182118 Score = 141 bits (355), Expect = 4e-32 Identities = 69/75 (92%), Positives = 72/75 (96%) Frame = -1 Query: 1 MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGL 60 MG+LSVDLLITLQILPVFFSNCLFLALYDSVILLKH+VL LSRSKS RGEWRRMLTSEGL Sbjct: 1194210 MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHMVLFLSRSKSARGEWRRMLTSEGL 1194031 Query: 61 RCVWKSFLLDAYKQL 75 RCVW SFLLDAYKQ+ Sbjct: 1194030 RCVWNSFLLDAYKQV 1193986 >gi|397456552|gb|JH738948.1| Alligator mississippiensis unplaced genomic scaffold scaffold-13070, whole genome shotgun sequence Length = 167594 Score = 150 bits (378), Expect = 9e-35 Identities = 89/221 (40%), Positives = 124/221 (56%) Frame = +2 Query: 89 LXLVYEAYKSRLLVYSHLDLWMVKLGEDAPNSSVVHVSSTEGGDNSGNGTQEKIAEGATC 148 L V+ +K L +H+ G APN V+ + Q+++ Sbjct: 61235 LKAVWHGHKLDLFKAAHV-------GARAPNPEVIRLDG-----------QKRL------ 61342 Query: 149 HLLDFASPERPLVVNFGSATXPPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPSDGWAI 208 LLDFA +RPLV+NFGS T PPF ++L AF++L F +ADFLLVYI+EAHPSDGW Sbjct: 61343 RLLDFARGKRPLVLNFGSCT*PPFMARLRAFQRLAAHFLDIADFLLVYIEEAHPSDGWV- 61519 Query: 209 PGDSSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGVAFERVCI 268 S ++++ KHQ +DR AAQ + E P C + D MDN ++ AYG FER+ I Sbjct: 61520 --SSDAAYKIPKHQCLQDRLRAAQLMREG---APGCPLAVDTMDNASSAAYGAYFERLYI 61684 Query: 269 VQRQKIAYLGGKGPFSYNLQEVRHWLEKNFSKRXKKTRLAG 309 VQ +++ Y GG+GP Y + E+R WL++ KTRL G Sbjct: 61685 VQEERVMYQGGRGPEGYKISELRSWLDQ------YKTRLRG 61789 >gi|397464218|gb|JH731283.1| Alligator mississippiensis unplaced genomic scaffold scaffold-461, whole genome shotgun sequence Length = 1270512 Score = 60.8 bits (146), Expect = 7e-08 Identities = 32/59 (54%), Positives = 39/59 (66%), Gaps = 2/59 (3%) Frame = -2 Query: 145 GATCHLLDFASP--ERPLVVNFGSATXPPFTSQLPAFRKLVEEFSSVADFLLVYIDEAH 201 G C + F S RPLV+NFGS T P F + F KLVE+F+ VADFLL+YI+EAH Sbjct: 1131371 GFKCLTIYFMSVLGNRPLVLNFGSCT*PSFLLKFDEFNKLVEDFNPVADFLLIYIEEAH 1131195 Score = 48.9 bits (115), Expect = 3e-04 Identities = 28/73 (38%), Positives = 41/73 (56%) Frame = -3 Query: 204 DGWAIPGDSSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGVAF 263 DGWA + +K HQN EDR AA+ LL++ P C VV D M+N ++ Y Sbjct: 1128880 DGWAFKNN----IVIKNHQNLEDRKMAARFLLKK---NPLCPVVVDTMENLSSSKYAALP 1128722 Query: 264 ERVCIVQRQKIAY 276 ER+ ++Q +K+ Y Sbjct: 1128721 ERLYLLQGRKVVY 1128683 >gi|397458870|gb|JH736631.1| Alligator mississippiensis unplaced genomic scaffold scaffold-9144, whole genome shotgun sequence Length = 140901 Score = 36.2 bits (82), Expect = 1.8 Identities = 15/42 (35%), Positives = 22/42 (52%) Frame = +2 Query: 51 WRRMLTSEGLRCVWKSFLLDAYKQLNCPPSGFSKDGHILXLV 92 W ++ S+GLR W S ++ NC G KDG +L +V Sbjct: 138107 WPKLARSKGLRRTWPSLRTPSHDTFNCLKGGLKKDGAVLSVV 138232 >gi|397457284|gb|JH738216.1| Alligator mississippiensis unplaced genomic scaffold scaffold-11811, whole genome shotgun sequence Length = 253508 Score = 34.3 bits (77), Expect = 7.0 Identities = 25/78 (32%), Positives = 34/78 (43%), Gaps = 4/78 (5%) Frame = +2 Query: 172 FTSQLPAFRKLVEEFSSVADFLLVYIDEAHPSDGWAIPGDSSLSFEVKKHQNQEDRC--- 228 +TSQ ++ E S + + V+ H S W P SSL + +H D C Sbjct: 144782 WTSQPVIVCRMGGEKGSGGNSVWVFFSTPHFSTSWGAPRTSSLGSDNNEHWQSHDHCYGN 144961 Query: 229 -AAAQQLLERFSLPPQCR 245 AA Q + RF LPP R Sbjct: 144962 LAAGSQPMARF-LPPAFR 145012 >gi|397463755|gb|JH731746.1| Alligator mississippiensis unplaced genomic scaffold scaffold-1211, whole genome shotgun sequence Length = 888356 Score = 33.9 bits (76), Expect = 9.1 Identities = 20/59 (33%), Positives = 32/59 (54%), Gaps = 3/59 (5%) Frame = -3 Query: 78 PPSGFSKDGHILXLVYEAYKSRLLVYSH---LDLWMVKLGEDAPNSSVVHVSSTEGGDN 133 PP K H L ++ E Y + +LVY H L++++ +L E P + V HV + GD+ Sbjct: 843963 PPCFLKKHSHQL-IM*EKYGASILVYKHLESLEMFLAELRESVP*TLV*HVHKSHSGDS 843790 Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis /genome.fa Posted date: Oct 18, 2013 5:53 PM Number of letters in database: 2,174,243,242 Number of sequences in database: 14,644 Lambda K H 0.321 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 940,798,199 Number of Sequences: 14644 Number of extensions: 15718097 Number of successful extensions: 60512 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 689 Number of HSP's successfully gapped in prelim test: 502 Number of HSP's that attempted gapping in prelim test: 56399 Number of HSP's gapped (non-prelim): 11467 length of query: 309 length of database: 724,747,747 effective HSP length: 129 effective length of query: 180 effective length of database: 722,858,671 effective search space: 130114560780 effective search space used: 130114560780 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 76 (33.9 bits)