TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000002_2.0 # Protein # Iodothyronine deiodinase 1 (DI1) #
Human (Homo sapiens)
(249 letters)
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g
enome.fa
14,644 sequences; 2,174,243,242 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|397456552|gb|JH738948.1| Alligator mississippiensis unplaced ... 159 1e-37
gi|397463745|gb|JH731756.1| Alligator mississippiensis unplaced ... 140 5e-32
gi|397464218|gb|JH731283.1| Alligator mississippiensis unplaced ... 98 3e-19
gi|397460945|gb|JH734556.1| Alligator mississippiensis unplaced ... 36 1.6
gi|397463738|gb|JH731763.1| Alligator mississippiensis unplaced ... 34 4.8
gi|397459299|gb|JH736202.1| Alligator mississippiensis unplaced ... 33 8.1
>gi|397456552|gb|JH738948.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-13070, whole genome shotgun sequence
Length = 167594
Score = 159 bits (401), Expect = 1e-37
Identities = 84/161 (52%), Positives = 106/161 (65%), Gaps = 2/161 (1%)
Frame = +2
Query: 88 LGGLAPNCPVVRLSGQ-RCNIWEFMQGNRPLVLNFGSCTXPSFMFKFDQFKRLIEDFSSI 146
+G APN V+RL GQ R + +F +G RPLVLNFGSCT P FM + F+RL F I
Sbjct: 61286 VGARAPNPEVIRLDGQKRLRLLDFARGKRPLVLNFGSCT*PPFMARLRAFQRLAAHFLDI 61465
Query: 147 ADFLVIYIEEAHASDGWAFKN-NMDIRNHQNLQDRLQAAHLLLARSPQCPVVVDTMQNQS 205
ADFL++YIEEAH SDGW + I HQ LQDRL+AA L+ +P CP+ VDTM N S
Sbjct: 61466 ADFLLVYIEEAHPSDGWVSSDAAYKIPKHQCLQDRLRAAQLMREGAPGCPLAVDTMDNAS 61645
Query: 206 SQLYAALPERLYIIQEGRILYKGKSGPWNYNPEEVRAVLEK 246
S Y A ERLYI+QE R++Y+G GP Y E+R+ L++
Sbjct: 61646 SAAYGAYFERLYIVQEERVMYQGGRGPEGYKISELRSWLDQ 61768
>gi|397463745|gb|JH731756.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-1225, whole genome shotgun sequence
Length = 1234326
Score = 140 bits (353), Expect = 5e-32
Identities = 78/200 (39%), Positives = 112/200 (56%), Gaps = 32/200 (16%)
Frame = -1
Query: 79 WQRLEDT--------TELGGLAPNCPVVRLS-----------------GQRCNIWEFMQG 113
WQR+ T +LGG APN V+ ++ G +C++ +F
Sbjct: 1182732 WQRVTVTLLSLFCHQVKLGGEAPNSRVIHITNGISGGSTRCKNVGGKLGSKCHLLDFANS 1182553
Query: 114 NRPLVLNFGSCTXPSFMFKFDQFKRLIEDFSSIADFLVIYIEEAHASDGWA----FKNNM 169
RPLV+NFGS T P F + F +L+E+FS +ADFL++YI+EAH SDGWA ++
Sbjct: 1182552 ERPLVVNFGSAT*PPFTSQLSAFSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSF 1182373
Query: 170 DIRNHQNLQDRLQAAHLLLAR---SPQCPVVVDTMQNQSSQLYAALPERLYIIQEGRILY 226
+++ H+N +DR AAH LL R PQC VV D M N ++ Y ER+ I+Q +I Y
Sbjct: 1182372 EVKKHKNQEDRCAAAHQLLQRFSLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAY 1182193
Query: 227 KGKSGPWNYNPEEVRAVLEK 246
G GP+ YN +EV+ LE+
Sbjct: 1182192 LGGKGPFFYNLQEVQLWLEQ 1182133
>gi|397464218|gb|JH731283.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-461, whole genome shotgun sequence
Length = 1270512
Score = 98.2 bits (243), Expect = 3e-19
Identities = 44/67 (65%), Positives = 55/67 (82%)
Frame = -3
Query: 161 DGWAFKNNMDIRNHQNLQDRLQAAHLLLARSPQCPVVVDTMQNQSSQLYAALPERLYIIQ 220
DGWAFKNN+ I+NHQNL+DR AA LL ++P CPVVVDTM+N SS YAALPERLY++Q
Sbjct: 1128880 DGWAFKNNIVIKNHQNLEDRKMAARFLLKKNPLCPVVVDTMENLSSSKYAALPERLYLLQ 1128701
Query: 221 EGRILYK 227
+++YK
Sbjct: 1128700 GRKVVYK 1128680
Score = 84.7 bits (208), Expect = 3e-15
Identities = 41/67 (61%), Positives = 51/67 (76%), Gaps = 2/67 (2%)
Frame = -2
Query: 96 PVVRLSGQRCNIWEFMQ--GNRPLVLNFGSCTXPSFMFKFDQFKRLIEDFSSIADFLVIY 153
P + G +C FM GNRPLVLNFGSCT PSF+ KFD+F +L+EDF+ +ADFL+IY
Sbjct: 1131389 PFIGGLGFKCLTIYFMSVLGNRPLVLNFGSCT*PSFLLKFDEFNKLVEDFNPVADFLLIY 1131210
Query: 154 IEEAHAS 160
IEEAHA+
Sbjct: 1131209 IEEAHAT 1131189
Score = 73.6 bits (179), Expect = 7e-12
Identities = 32/52 (61%), Positives = 41/52 (78%)
Frame = -2
Query: 33 FPDRVKRNILAMGEKTGMTRNPHFSHDNWIPTFFSTQYFWFVLKVRWQRLED 84
FP+ VKR IL +G+K+ M RN FS++NW PTFFS +Y FVLKV+W+RLED
Sbjct: 1133117 FPEAVKRYILKLGQKSSMVRNSKFSYENWGPTFFSFKYLLFVLKVKWKRLED 1132962
Score = 39.7 bits (91), Expect = 0.11
Identities = 14/21 (66%), Positives = 20/21 (95%)
Frame = -3
Query: 227 KGKSGPWNYNPEEVRAVLEKL 247
+G +GPWNY+P+E+RA+LEKL
Sbjct: 1127833 QGGAGPWNYHPQEIRAILEKL 1127771
>gi|397460945|gb|JH734556.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-5742, whole genome shotgun sequence
Length = 1129154
Score = 35.8 bits (81), Expect = 1.6
Identities = 22/81 (27%), Positives = 37/81 (45%), Gaps = 10/81 (12%)
Frame = +1
Query: 63 PTFFSTQYFW------FVLKVRWQRLEDTTE----LGGLAPNCPVVRLSGQRCNIWEFMQ 112
P F +YFW +L+ + +++ + LG + +CPV + S ++ +
Sbjct: 269743 PALFYHEYFW*LCKDKLILRGKQRKMRKKSLCSGILGHFSSSCPVFKTSPRQMVCFADFP 269922
Query: 113 GNRPLVLNFGSCTXPSFMFKF 133
G L LNFG+ P F F F
Sbjct: 269923 GRMVLFLNFGTLLLPLFSFMF 269985
>gi|397463738|gb|JH731763.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-1236, whole genome shotgun sequence
Length = 1982358
Score = 34.3 bits (77), Expect = 4.8
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +2
Query: 72 WFVLKVRWQRLEDTTELGGLAPNCPVVRLSGQRC 105
W +L W + L AP+CPVVR G+RC
Sbjct: 540083 WLLLHWEWDQEVAKAALAAAAPHCPVVRTLGRRC 540184
>gi|397459299|gb|JH736202.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-8447, whole genome shotgun sequence
Length = 380716
Score = 33.5 bits (75), Expect = 8.1
Identities = 23/61 (37%), Positives = 31/61 (50%), Gaps = 3/61 (4%)
Frame = +2
Query: 89 GGLAPNCPVVRLS--GQRCNIWEFMQGNRPLVLNFGSCTXPSFMFKFDQ-FKRLIEDFSS 145
GGL P P +R+ G RC++ G PL+L F + P +F Q F RL FSS
Sbjct: 287222 GGLGPRSPSIRIFP*GFRCHLAVSRLGFLPLILQFRAVPFPMSLFSAIQAFIRLYLAFSS 287401
Query: 146 I 146
+
Sbjct: 287402 L 287404
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis
/genome.fa
Posted date: Oct 18, 2013 5:53 PM
Number of letters in database: 2,174,243,242
Number of sequences in database: 14,644
Lambda K H
0.324 0.138 0.448
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 751,583,552
Number of Sequences: 14644
Number of extensions: 11409933
Number of successful extensions: 63467
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 818
Number of HSP's successfully gapped in prelim test: 482
Number of HSP's that attempted gapping in prelim test: 58669
Number of HSP's gapped (non-prelim): 14083
length of query: 249
length of database: 724,747,747
effective HSP length: 126
effective length of query: 123
effective length of database: 722,902,603
effective search space: 88917020169
effective search space used: 88917020169
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 74 (33.1 bits)