TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000127_2.0 # Protein # Selenoprotein W2 (SelW2) # Human (Homo sapiens) (115 letters) Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno me.fa 16,710 sequences; 3,156,886,159 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|472267550|gb|KB717107.1| Leptonychotes weddellii isolate WS11... 86 9e-18 >gi|472267550|gb|KB717107.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold02401, whole genome shotgun sequence Length = 401892 Score = 85.5 bits (210), Expect = 5e-16 Identities = 52/97 (53%), Positives = 52/97 (53%), Gaps = 34/97 (35%) Frame = +2 Query: 1 MSGEPGQTSVAXXXXXXXXGSGVRIVVEY------------------------------- 29 MSGEPG TS A GSGVRIVVEY Sbjct: 18659 MSGEPGPTSEAPPPGEIEPGSGVRIVVEYW*ASPACGVSGPNGGGSTGGPSPETQAEPKR 18838 Query: 30 ---CEPCGFEATYLELASAVKEQYPGIEIESRLGGTG 63 EPCGFEATYLELASAVKEQYPGIEIESRLGGTG Sbjct: 18839 LVPSEPCGFEATYLELASAVKEQYPGIEIESRLGGTG 18949 Score = 57.8 bits (138), Expect(2) = 9e-18 Identities = 26/27 (96%), Positives = 27/27 (100%) Frame = +1 Query: 63 GAFEIEINGQLVFSKLENGGFPYEKDL 89 GAFEIEINGQLVFSKLENGGFPYEKD+ Sbjct: 19393 GAFEIEINGQLVFSKLENGGFPYEKDV 19473 Score = 53.9 bits (128), Expect(2) = 9e-18 Identities = 26/27 (96%), Positives = 26/27 (96%) Frame = +2 Query: 89 LIEAIRRASNGETLEKITNSRPPCVIL 115 LIEAIRRASNGE LEKITNSRPPCVIL Sbjct: 19550 LIEAIRRASNGEPLEKITNSRPPCVIL 19630 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 3,087,506,849 Number of sequences in database: 5788 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa.01 Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 69,379,310 Number of sequences in database: 10,922 Lambda K H 0.314 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 361,414,324 Number of Sequences: 16710 Number of extensions: 3450906 Number of successful extensions: 7357 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 244 Number of HSP's successfully gapped in prelim test: 71 Number of HSP's that attempted gapping in prelim test: 6981 Number of HSP's gapped (non-prelim): 720 length of query: 115 length of database: 1,052,295,386 effective HSP length: 90 effective length of query: 25 effective length of database: 1,050,791,486 effective search space: 26269787150 effective search space used: 26269787150 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 70 (31.6 bits)