TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000114_2.0 # Protein # Selenoprotein U2 (SelU2) # Human (Homo sapiens) (226 letters) Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno me.fa 16,710 sequences; 3,156,886,159 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|472268960|gb|KB715697.1| Leptonychotes weddellii isolate WS11... 93 1e-17 gi|472268488|gb|KB716169.1| Leptonychotes weddellii isolate WS11... 33 9.5 >gi|472268960|gb|KB715697.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00991, whole genome shotgun sequence Length = 1075223 Score = 93.2 bits (230), Expect = 1e-17 Identities = 42/46 (91%), Positives = 46/46 (100%) Frame = -1 Query: 140 SGQSPHIKSNLLSGSLQSLWRAVTGPLFDFQGDPAQQGGTLILGPG 185 SG+SPHIKSN+LSGS++SLWRAVTGPLFDFQGDPAQQGGTLILGPG Sbjct: 380453 SGKSPHIKSNILSGSIRSLWRAVTGPLFDFQGDPAQQGGTLILGPG 380316 Score = 86.7 bits (213), Expect = 9e-16 Identities = 39/42 (92%), Positives = 42/42 (100%) Frame = -3 Query: 185 GNNIHFIHRDRNRLDHKPINSVLQLVGVQHVNFTNRPSVIHV 226 GNNIHFIHRD+NRLDHKPINSVLQLVGVQHV+FT+RPSVIHV Sbjct: 377817 GNNIHFIHRDKNRLDHKPINSVLQLVGVQHVDFTSRPSVIHV 377692 Score = 83.2 bits (204), Expect = 1e-14 Identities = 37/49 (75%), Positives = 44/49 (89%) Frame = -2 Query: 106 PFCKLTGYSHEIYVDPEREIYKRLGMKRGEEIASSGQSPHIKSNLLSGS 154 PFCKLTGYSHEIYVDPEREIYK+LGMKRGEEIA+SG++ H+ S +G+ Sbjct: 384715 PFCKLTGYSHEIYVDPEREIYKKLGMKRGEEIAASGKT*HVSSENANGA 384569 Score = 47.0 bits (110), Expect = 8e-04 Identities = 25/50 (50%), Positives = 29/50 (58%) Frame = -3 Query: 3 APAPVTRQVSGAAALVPAPSGPDSGQXXXXXXXXXXXXXXRGQRVPFGAL 52 A APVTRQVSG+AA PSGP+ GQ G++VPFGAL Sbjct: 386508 ALAPVTRQVSGSAAPAAVPSGPERGQPLAAAVAELPVLDASGRQVPFGAL 386359 Score = 47.0 bits (110), Expect = 8e-04 Identities = 20/21 (95%), Positives = 21/21 (100%) Frame = -3 Query: 67 LCYICKEYVEDLAKIPRSFLQ 87 LCYICKEYVEDLAKIP+SFLQ Sbjct: 385881 LCYICKEYVEDLAKIPKSFLQ 385819 Score = 40.4 bits (93), Expect = 0.078 Identities = 19/24 (79%), Positives = 21/24 (87%) Frame = -1 Query: 82 PRSFLQEANVTLIVIGQSSYHHIE 105 P L+EA+VTLIVIGQSSYHHIE Sbjct: 384947 PFFLLKEADVTLIVIGQSSYHHIE 384876 >gi|472268488|gb|KB716169.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold01463, whole genome shotgun sequence Length = 625042 Score = 33.5 bits (75), Expect = 9.5 Identities = 24/88 (27%), Positives = 38/88 (43%), Gaps = 3/88 (3%) Frame = +3 Query: 105 EPFCKLTGYSHEIYVDPEREIYKRLGMKRGEEIASSGQSPH---IKSNLLSGSLQSLWRA 161 E FC+L G+ E R G+E+ SG+ + ++ L + ++W+ Sbjct: 417633 EGFCRLQGFG-------EARCLGRGAATVGQEVGGSGRGTYPSCCPNSRLQKAEHAVWQN 417791 Query: 162 VTGPLFDFQGDPAQQGGTLILGPGNNIH 189 +GPL + G PA GTL G H Sbjct: 417792 SSGPLRCWAGAPAGDRGTLPAGASRLAH 417875 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 3,087,506,849 Number of sequences in database: 5788 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa.01 Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 69,379,310 Number of sequences in database: 10,922 Lambda K H 0.320 0.138 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 794,025,086 Number of Sequences: 16710 Number of extensions: 11451994 Number of successful extensions: 44851 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 481 Number of HSP's successfully gapped in prelim test: 341 Number of HSP's that attempted gapping in prelim test: 42292 Number of HSP's gapped (non-prelim): 7918 length of query: 226 length of database: 1,052,295,386 effective HSP length: 127 effective length of query: 99 effective length of database: 1,050,173,216 effective search space: 103967148384 effective search space used: 103967148384 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 75 (33.5 bits)