TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000067_2.0 # Protein # 15 kDa selenoprotein (Sel15) # Human (Homo sapiens) (162 letters) Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno me.fa 16,710 sequences; 3,156,886,159 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|472268923|gb|KB715734.1| Leptonychotes weddellii isolate WS11... 93 5e-18 gi|472269924|gb|KB714765.1| Leptonychotes weddellii isolate WS11... 37 0.47 >gi|472268923|gb|KB715734.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold01028, whole genome shotgun sequence Length = 828836 Score = 92.8 bits (229), Expect = 5e-18 Identities = 45/62 (72%), Positives = 47/62 (75%) Frame = +1 Query: 26 VSAFGAEFSSEACRELGFXXXXXXXXXXXXGQFNLLQLDPDCRGCCQEEAQFETKKLYAG 85 VSAFGAEFSSEACRELGF GQFNLLQLDPDCRGCCQEEAQFETKK+ Sbjct: 423241 VSAFGAEFSSEACRELGFSSNLLCSSCDLLGQFNLLQLDPDCRGCCQEEAQFETKKVLFS 423420 Query: 86 AI 87 +I Sbjct: 423421 SI 423426 Score = 88.2 bits (217), Expect = 1e-16 Identities = 43/43 (100%), Positives = 43/43 (100%) Frame = +2 Query: 120 YVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLERI 162 YVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLERI Sbjct: 468956 YVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLERI 469084 Score = 43.1 bits (100), Expect = 0.005 Identities = 20/21 (95%), Positives = 20/21 (95%) Frame = +3 Query: 82 LYAGAILEVCGUKLGRFPQVQ 102 LYAGAILEVCG KLGRFPQVQ Sbjct: 444282 LYAGAILEVCG*KLGRFPQVQ 444344 Score = 40.0 bits (92), Expect = 0.042 Identities = 21/27 (77%), Positives = 21/27 (77%) Frame = +2 Query: 1 MAAGPSGCLVPAFGLRLLLATVLQAVS 27 MAAGP G L AFGLRLLLA VLQ VS Sbjct: 414887 MAAGPGGLLPLAFGLRLLLAAVLQTVS 414967 Score = 38.5 bits (88), Expect = 0.12 Identities = 20/21 (95%), Positives = 20/21 (95%) Frame = +1 Query: 103 AFVRSDKPKLFRGLQIKYVRG 123 AFVRSDKPKLFRGLQIK VRG Sbjct: 466090 AFVRSDKPKLFRGLQIK-VRG 466149 >gi|472269924|gb|KB714765.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00059, whole genome shotgun sequence Length = 3449337 Score = 36.6 bits (83), Expect = 0.47 Identities = 40/106 (37%), Positives = 49/106 (46%) Frame = +2 Query: 16 RLLLATVLQAVSAFGAEFSSEACRELGFXXXXXXXXXXXXGQFNLLQLDPDCRGCCQEEA 75 RLLLAT+ A+SAFGA FSSEA + F LLQL P+ Q E Sbjct: 77060 RLLLATM**ALSAFGAVFSSEAPALVTF----------------LLQLAPN*GEHDQREV 77191 Query: 76 QFETKKLYAGAILEVCGUKLGRFPQVQAFVRSDKPKLFRGLQIKYV 121 + + +KL VC K + P + D RGLQIKYV Sbjct: 77192 RLDNRKL---GTCNVC-RKPRKLPSI-----CDSS---RGLQIKYV 77293 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 3,087,506,849 Number of sequences in database: 5788 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa.01 Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 69,379,310 Number of sequences in database: 10,922 Lambda K H 0.322 0.140 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 511,326,839 Number of Sequences: 16710 Number of extensions: 5540395 Number of successful extensions: 16561 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 240 Number of HSP's successfully gapped in prelim test: 87 Number of HSP's that attempted gapping in prelim test: 16036 Number of HSP's gapped (non-prelim): 1326 length of query: 162 length of database: 1,052,295,386 effective HSP length: 121 effective length of query: 41 effective length of database: 1,050,273,476 effective search space: 43061212516 effective search space used: 43061212516 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 72 (32.3 bits)