Command line: [exonerate -m p2g --showtargetgff -q ./eEFSec.fa -t eEFSec.carpeta/genomic.fa] Hostname: [ws101135] C4 Alignment: ------------ Query: SPP00000144_2.0 # Protein # Eukaryotic elongation factor (eEFSec) # Walrus (Odobenus rosmarus) Target: gi|472269049|gb|KB715608.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00902, whole genome shotgun sequence Model: protein2genome:local Raw score: 1661 Query range: 261 -> 595 Target range: 513387 -> 576521 262 : LysValValLysLysValLysSerMetGlnMetPheHisMetProValThrSerAlaM : 281 :::||||||||||||||||||||||||||||||||||||||||||||||||||||||| GlnValValLysLysValLysSerMetGlnMetPheHisMetProValThrSerAlaM 513388 : CAGGTGGTGAAGAAGGTGAAGTCCATGCAGATGTTTCACATGCCTGTCACCTCAGCCA : 513445 282 : etGlnGlyAspArgLeuGlyIleCysValThrGlnPheAspProLysLeuLeuGluAr : 300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| etGlnGlyAspArgLeuGlyIleCysValThrGlnPheAspProLysLeuLeuGluAr 513446 : TGCAGGGAGACCGACTGGGCATCTGCGTCACCCAGTTTGACCCCAAGCTACTGGAGCG : 513502 301 : gGlyLeuValCysAlaProGluSerLeuHisThrValHisAlaAlaIleIleSerVal : 319 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| gGlyLeuValCysAlaProGluSerLeuHisThrValHisAlaAlaIleIleSerVal 513503 : TGGCCTGGTGTGTGCCCCTGAGTCCCTGCACACTGTCCACGCAGCCATCATCTCTGTG : 513559 320 : GluLysIleProTyrPheArgGlyProLeuGlnThrLysAlaLysPheHisIleThrV : 339 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| GluLysIleProTyrPheArgGlyProLeuGlnThrLysAlaLysPheHisIleThrV 513560 : GAGAAGATCCCGTATTTCCGGGGGCCCCTGCAGACCAAAGCCAAGTTCCACATCACGG : 513619 340 : alGlyHisGluThrValMetGlyArgLeuMetPhePheSerLeuAlaProAspSerPh : 358 ||||||||||||||||||||||||||||||||||||||||| |||||||||||||| alGlyHisGluThrValMetGlyArgLeuMetPhePheSerProAlaProAspSerPh 513620 : TGGGCCATGAGACAGTCATGGGCCGGTTGATGTTCTTCAGCCCTGCCCCCGATAGCTT : 513676 359 : eAspHisGluProValLeuAspSerPheAspPheSerArgGluTyrLeuPheGlnGlu : 377 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| eAspHisGluProValLeuAspSerPheAspPheSerArgGluTyrLeuPheGlnGlu 513677 : TGACCATGAGCCTGTGCTGGACTCCTTCGACTTCTCTCGAGAATACCTCTTCCAGGAG : 513733 378 : GlnTyrLeuAspLysAspLeuAlaProValAlaThrAspSerAspGluAlaAsnLysL : 397 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| GlnTyrLeuAspLysAspLeuAlaProValAlaThrAspSerAspGluAlaAsnLysL 513734 : CAGTACCTAGATAAGGATTTGGCACCAGTGGCGACAGACAGTGATGAGGCTAACAAGA : 513793 398 : ysThrGlyGlnAlaThrGluGlyCysCysProArgGlnGlnTrpAlaLeuValGluPh : 416 ||||||||||||||||||||||| |||||||||||||||||||||||||||||||| ysThrGlyGlnAlaThrGluGlyArgCysProArgGlnGlnTrpAlaLeuValGluPh 513794 : AGACGGGCCAGGCTACAGAAGGCCGCTGCCCTCGGCAGCAGTGGGCCCTCGTGGAATT : 513850 417 : eGluLysProValThrCysProArgLeuCysLeuValIleGlySerArgLeuAspAla : 435 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| eGluLysProValThrCysProArgLeuCysLeuValIleGlySerArgLeuAspAla 513851 : TGAGAAGCCTGTCACCTGCCCTCGGCTGTGCCTGGTGATTGGCTCCAGGCTAGATGCG : 513907 436 : AspIleHisAlaAsnMetCysArgLeuAlaPheHisGlyValLeuLeuHisGlyLeuG : 455 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| AspIleHisAlaAsnMetCysArgLeuAlaPheHisGlyValLeuLeuHisGlyLeuG 513908 : GACATTCATGCCAACATGTGCCGACTGGCCTTCCATGGTGTCCTGCTCCATGGGCTGG : 513967 456 : luAspLysAspTyrAlaGluSerPheLeuProArgLeuLysValTyrLysLeuLysHi : 474 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| luAspLysAspTyrAlaGluSerPheLeuProArgLeuLysValTyrLysLeuLysHi 513968 : AGGACAAGGACTATGCTGAGAGCTTCTTGCCCAGGCTGAAAGTGTATAAGCTCAAGCA : 514024 475 : sLysHisGlyLeuValGluArg >>>> Target Intron 1 >>>> ValMetA : 484 |||||||||||||||||||||| 16667 bp ||||||| sLysHisGlyLeuValGluArg++ ++ValMetA 514025 : CAAACATGGCCTTGTAGAACGGgt.........................agGTGATGG : 530721 485 : spAspHisSerValIleGlyArgSerLeuPheLysLysGluThrAsnIleGlnLeuPh : 503 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| spAspHisSerValIleGlyArgSerLeuPheLysLysGluThrAsnIleGlnLeuPh 530722 : ATGACCACAGCGTGATCGGCCGCTCCCTGTTCAAAAAGGAGACCAACATCCAGCTCTT : 530778 504 : eValGlyLeuLysValTyrLeuSerThrGlyGluLeuGlyValIleAspSerAlaPhe : 522 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| eValGlyLeuLysValTyrLeuSerThrGlyGluLeuGlyValIleAspSerAlaPhe 530779 : TGTGGGCCTCAAGGTGTACCTATCCACCGGGGAGCTGGGGGTCATCGACAGCGCCTTC : 530835 523 : GlyGlnSerGlyLysPheLysIleHisIlePro{G} >>>> Target Intron 2 : 534 |||||||||||||||||||||||||||||||||{|} 45462 bp GlyGlnSerGlyLysPheLysIleHisIlePro{G}++ 530836 : GGCCAGAGTGGCAAGTTCAAGATCCACATCCCT{G}gt.................... : 530874 535 : >>>> {ly}GlyLeuSerSerGluSerLysLysIleLeuThrProThrLeuLysLy : 550 {||}||||||||| ||||||||||||||||||||||||||||||||||| ++{ly}GlyLeuSerProGluSerLysLysIleLeuThrProThrLeuLysLy 530875 : .....ag{GC}GGCCTCAGCCCCGAGTCCAAGAAGATTCTAACACCCACCCTCAAGAA : 576381 551 : sArgGlyArgAlaGlyArg<->GluAlaAlaLysLeuGluGluLeuAlaGluArgSer : 568 ||||||||||||||||||| |||||||||||| ||||||:::|||||||||||| sArgGlyArgAlaGlyArgGlyGluAlaAlaLysGlnGluGluValAlaGluArgSer 576382 : GCGGGGCCGGGCCGGCCGAGGAGAAGCAGCCAAGCAGGAGGAGGTGGCCGAGCGGTCT : 576438 569 : GluProAlaGlnHisValAlaLeuSerLeuSerPheLysArgTyrValPheAspThrH : 588 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| GluProAlaGlnHisValAlaLeuSerLeuSerPheLysArgTyrValPheAspThrH 576439 : GAGCCTGCGCAGCACGTGGCCCTCAGCCTGTCTTTCAAGCGTTACGTCTTTGACACCC : 576498 589 : isLysArgMetValGlnSerPro : 595 ||||||||||||||||||||||| isLysArgMetValGlnSerPro 576499 : ACAAGCGCATGGTCCAGTCTCCC : 576521 vulgar: SPP00000144_2.0 261 595 . gi|472269049|gb|KB715608.1| 513387 576521 + 1661 M 220 660 5 0 2 I 0 16663 3 0 2 M 52 156 S 0 1 5 0 2 I 0 45458 3 0 2 S 1 2 M 22 66 G 0 3 M 39 117 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-20 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|472269049|gb|KB715608.1| exonerate:protein2genome:local gene 513388 576521 1661 + . gene_id 1 ; sequence SPP00000144_2.0 ; gene_orientation + gi|472269049|gb|KB715608.1| exonerate:protein2genome:local cds 513388 514047 . + . gi|472269049|gb|KB715608.1| exonerate:protein2genome:local exon 513388 514047 . + . insertions 0 ; deletions 0 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice5 514048 514049 . + . intron_id 1 ; splice_site "GT" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local intron 514048 530714 . + . intron_id 1 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice3 530713 530714 . + . intron_id 0 ; splice_site "AG" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local cds 530715 530871 . + . gi|472269049|gb|KB715608.1| exonerate:protein2genome:local exon 530715 530871 . + . insertions 0 ; deletions 0 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice5 530872 530873 . + . intron_id 2 ; splice_site "GT" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local intron 530872 576333 . + . intron_id 2 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice3 576332 576333 . + . intron_id 1 ; splice_site "AG" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local cds 576334 576521 . + . gi|472269049|gb|KB715608.1| exonerate:protein2genome:local exon 576334 576521 . + . insertions 3 ; deletions 0 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local similarity 513388 576521 1661 + . alignment_id 1 ; Query SPP00000144_2.0 ; Align 513388 262 660 ; Align 530715 482 156 ; Align 576336 535 66 ; Align 576405 557 117 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000144_2.0 # Protein # Eukaryotic elongation factor (eEFSec) # Walrus (Odobenus rosmarus) Target: gi|472269049|gb|KB715608.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00902, whole genome shotgun sequence Model: protein2genome:local Raw score: 512 Query range: 71 -> 197 Target range: 328562 -> 587204 72 : ProAlaAlaSerGlyAlaGluProGlyProGlyGluProGlnLeuGlnValThrLeuV : 91 |||||||||||||||||||||||| ||||||||||||||||||||||||||||||| ProAlaAlaSerGlyAlaGluProGluProGlyGluProGlnLeuGlnValThrLeuV 328563 : CCGGCGGCGTCCGGAGCCGAGCCCGAGCCCGGCGAGCCGCAGCTTCAGGTCACGCTGG : 328620 92 : alAspCysProGlyHisAlaSerLeuIleArgThrIleIleGly{G} >>>> Targ : 106 ||||||||||||||||||||||||||||||||||||||||||||{|} alAspCysProGlyHisAlaSerLeuIleArgThrIleIleGly{G}++ 328621 : TCGACTGCCCCGGGCACGCCTCCCTCATCCGGACCATCATTGGC{G}gt......... : 328668 107 : et Intron 1 >>>> {ly}AlaGlnIleIleAspLeuMetMetLeuValIleAsp : 118 84926 bp {||}|||||||||||||||||||||||||||||||||||| ++{ly}AlaGlnIleIleAspLeuMetMetLeuValIleAsp 328669 : ................ag{GG}GCCCAGATCATTGATCTGATGATGCTGGTCATTGAT : 413627 119 : ValThrLysGlyMetGlnThrGlnSerAlaGluCysLeuValIleGlyGlnIleAlaC : 138 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ValThrLysGlyMetGlnThrGlnSerAlaGluCysLeuValIleGlyGlnIleAlaC 413628 : GTGACCAAAGGGATGCAGACCCAGTCAGCGGAGTGCCTTGTGATTGGGCAGATCGCCT : 413687 139 : ysGlnLysLeuValValValLeuAsnLysIleAspLeuLeuAlaGluGlyLysArgGl : 157 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ysGlnLysLeuValValValLeuAsnLysIleAspLeuLeuAlaGluGlyLysArgGl 413688 : GCCAGAAGCTGGTTGTGGTGCTGAACAAAATAGACCTCTTAGCTGAAGGGAAGAGACA : 413744 158 : nAlaAlaIleAspLysMetThrLysLysMetGlnArgThrLeuGluAsnThr{Ly} : 175 ||||||||||||||||||||||||||||||||||||||||||||||||||||{||} nAlaAlaIleAspLysMetThrLysLysMetGlnArgThrLeuGluAsnThr{Ly}++ 413745 : GGCAGCGATTGATAAAATGACCAAGAAAATGCAGAGGACCCTGGAGAACACC{AA}gt : 413800 176 : >>>> Target Intron 2 >>>> {s}PheArgGlyAlaProIleIleProValA : 185 173338 bp {|}::: ... :::: +-{s}TyrLeuAlaTyrLeuProProLeuLeuS 413801 : .........................aa{A}TATCTTGCTTATTTACCTCCCTTGTTGT : 587166 186 : laAlaLysProGlyGlyProGluAlaProGluThrGlu : 197 ::|||...||| ||| ||||||||||||||| erAlaSerProThrGlyThr***AlaProGluThrGlu 587167 : CAGCGTCCCCCACGGGAACATGAGCTCCAGAGACAGAA : 587204 vulgar: SPP00000144_2.0 71 197 . gi|472269049|gb|KB715608.1| 328562 587204 + 512 M 34 102 S 0 1 5 0 2 I 0 84922 3 0 2 S 1 2 M 68 204 S 0 2 5 0 2 I 0 173334 3 0 2 S 1 1 M 22 66 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-20 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|472269049|gb|KB715608.1| exonerate:protein2genome:local gene 328563 587204 512 + . gene_id 2 ; sequence SPP00000144_2.0 ; gene_orientation + gi|472269049|gb|KB715608.1| exonerate:protein2genome:local cds 328563 328665 . + . gi|472269049|gb|KB715608.1| exonerate:protein2genome:local exon 328563 328665 . + . insertions 0 ; deletions 0 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice5 328666 328667 . + . intron_id 1 ; splice_site "GT" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local intron 328666 413591 . + . intron_id 1 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice3 413590 413591 . + . intron_id 0 ; splice_site "AG" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local cds 413592 413799 . + . gi|472269049|gb|KB715608.1| exonerate:protein2genome:local exon 413592 413799 . + . insertions 0 ; deletions 0 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice5 413800 413801 . + . intron_id 2 ; splice_site "GT" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local intron 413800 587137 . + . intron_id 2 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice3 587136 587137 . + . intron_id 1 ; splice_site "AA" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local cds 587138 587204 . + . gi|472269049|gb|KB715608.1| exonerate:protein2genome:local exon 587138 587204 . + . insertions 0 ; deletions 0 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local similarity 328563 587204 512 + . alignment_id 2 ; Query SPP00000144_2.0 ; Align 328563 72 102 ; Align 413594 107 204 ; Align 587139 176 66 # --- END OF GFF DUMP --- # -- completed exonerate analysis