Command line: [exonerate -m p2g --showtargetgff -q ./eEFSec.fa -t eEFSec.carpeta/genomic.fa] Hostname: [ws101135] C4 Alignment: ------------ Query: SPP00000144_2.0 # Protein # Eukaryotic elongation factor (eEFSec) # Human (Homo sapiens) Target: gi|472269049|gb|KB715608.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00902, whole genome shotgun sequence Model: protein2genome:local Raw score: 1589 Query range: 261 -> 596 Target range: 513387 -> 576521 262 : LysValValLysLysValLysSerMetGlnMetPheHisMetProIleThrSerAlaM : 281 :!!||||||||||||||||||||||||||||||||||||||||||:!!|||||||||| GlnValValLysLysValLysSerMetGlnMetPheHisMetProValThrSerAlaM 513388 : CAGGTGGTGAAGAAGGTGAAGTCCATGCAGATGTTTCACATGCCTGTCACCTCAGCCA : 513445 282 : etGlnGlyAspArgLeuGlyIleCysValThrGlnPheAspProLysLeuLeuGluAr : 300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| etGlnGlyAspArgLeuGlyIleCysValThrGlnPheAspProLysLeuLeuGluAr 513446 : TGCAGGGAGACCGACTGGGCATCTGCGTCACCCAGTTTGACCCCAAGCTACTGGAGCG : 513502 301 : gGlyLeuValCysAlaProGluSerLeuHisThrValHisAlaAlaLeuIleSerVal : 319 ||||||||||||||||||||||||||||||||||||||||||||||:!!||||||||| gGlyLeuValCysAlaProGluSerLeuHisThrValHisAlaAlaIleIleSerVal 513503 : TGGCCTGGTGTGTGCCCCTGAGTCCCTGCACACTGTCCACGCAGCCATCATCTCTGTG : 513559 320 : GluLysIleProTyrPheArgGlyProLeuGlnThrLysAlaLysPheHisIleThrV : 339 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| GluLysIleProTyrPheArgGlyProLeuGlnThrLysAlaLysPheHisIleThrV 513560 : GAGAAGATCCCGTATTTCCGGGGGCCCCTGCAGACCAAAGCCAAGTTCCACATCACGG : 513619 340 : alGlyHisGluThrValMetGlyArgLeuMetPhePheSerProAlaProAspAsnPh : 358 |||||||||||||||||||||||||||||||||||||||||||||||||||||!:!|| alGlyHisGluThrValMetGlyArgLeuMetPhePheSerProAlaProAspSerPh 513620 : TGGGCCATGAGACAGTCATGGGCCGGTTGATGTTCTTCAGCCCTGCCCCCGATAGCTT : 513676 359 : eAspGlnGluProIleLeuAspSerPheAsnPheSerGlnGluTyrLeuPheGlnGlu : 377 ||||!!.||||||:!:||||||||||||:!!||||||!:!|||||||||||||||||| eAspHisGluProValLeuAspSerPheAspPheSerArgGluTyrLeuPheGlnGlu 513677 : TGACCATGAGCCTGTGCTGGACTCCTTCGACTTCTCTCGAGAATACCTCTTCCAGGAG : 513733 378 : GlnTyrLeuSerLysAspLeuThrProAlaValThrAspAsnAspGluAlaAspLysL : 397 |||||||||..!|||||||||.!!|||!.!!.!||||||!:!|||||||||:!!|||| GlnTyrLeuAspLysAspLeuAlaProValAlaThrAspSerAspGluAlaAsnLysL 513734 : CAGTACCTAGATAAGGATTTGGCACCAGTGGCGACAGACAGTGATGAGGCTAACAAGA : 513793 398 : ysAlaGlyGlnAlaThrGluGlyHisCysProArgGlnGlnTrpAlaLeuValGluPh : 416 ||.!!||||||||||||||||||!.!|||||||||||||||||||||||||||||||| ysThrGlyGlnAlaThrGluGlyArgCysProArgGlnGlnTrpAlaLeuValGluPh 513794 : AGACGGGCCAGGCTACAGAAGGCCGCTGCCCTCGGCAGCAGTGGGCCCTCGTGGAATT : 513850 417 : eGluLysProValThrCysProArgLeuCysLeuValIleGlySerArgLeuAspAla : 435 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| eGluLysProValThrCysProArgLeuCysLeuValIleGlySerArgLeuAspAla 513851 : TGAGAAGCCTGTCACCTGCCCTCGGCTGTGCCTGGTGATTGGCTCCAGGCTAGATGCG : 513907 436 : AspIleHisThrAsnThrCysArgLeuAlaPheHisGlyIleLeuLeuHisGlyLeuG : 455 |||||||||.!!|||! !|||||||||||||||||||||:!!|||||||||||||||| AspIleHisAlaAsnMetCysArgLeuAlaPheHisGlyValLeuLeuHisGlyLeuG 513908 : GACATTCATGCCAACATGTGCCGACTGGCCTTCCATGGTGTCCTGCTCCATGGGCTGG : 513967 456 : luAspArgAsnTyrAlaAspSerPheLeuProArgLeuLysValTyrLysLeuLysHi : 474 |||||!:!:!!||||||!!:|||||||||||||||||||||||||||||||||||||| luAspLysAspTyrAlaGluSerPheLeuProArgLeuLysValTyrLysLeuLysHi 513968 : AGGACAAGGACTATGCTGAGAGCTTCTTGCCCAGGCTGAAAGTGTATAAGCTCAAGCA : 514024 475 : sLysHisGlyLeuValGluArg >>>> Target Intron 1 >>>> AlaMetA : 484 |||||||||||||||||||||| 16667 bp !.!|||| sLysHisGlyLeuValGluArg++ ++ValMetA 514025 : CAAACATGGCCTTGTAGAACGGgt.........................agGTGATGG : 530721 485 : spAspTyrSerValIleGlyArgSerLeuPheLysLysGluThrAsnIleGlnLeuPh : 503 |||||:!!|||||||||||||||||||||||||||||||||||||||||||||||||| spAspHisSerValIleGlyArgSerLeuPheLysLysGluThrAsnIleGlnLeuPh 530722 : ATGACCACAGCGTGATCGGCCGCTCCCTGTTCAAAAAGGAGACCAACATCCAGCTCTT : 530778 504 : eValGlyLeuLysValHisLeuSerThrGlyGluLeuGlyIleIleAspSerAlaPhe : 522 ||||||||||||||||:!!|||||||||||||||||||||:!!||||||||||||||| eValGlyLeuLysValTyrLeuSerThrGlyGluLeuGlyValIleAspSerAlaPhe 530779 : TGTGGGCCTCAAGGTGTACCTATCCACCGGGGAGCTGGGGGTCATCGACAGCGCCTTC : 530835 523 : GlyGlnSerGlyLysPheLysIleHisIlePro{G} >>>> Target Intron 2 : 534 |||||||||||||||||||||||||||||||||{|} 45462 bp GlyGlnSerGlyLysPheLysIleHisIlePro{G}++ 530836 : GGCCAGAGTGGCAAGTTCAAGATCCACATCCCT{G}gt.................... : 530874 535 : >>>> {ly}GlyLeuSerProGluSerLysLysIleLeuThrProAlaLeuLysLy : 550 {||}||||||||||||||||||||||||||||||||||||.!!|||||||| ++{ly}GlyLeuSerProGluSerLysLysIleLeuThrProThrLeuLysLy 530875 : .....ag{GC}GGCCTCAGCCCCGAGTCCAAGAAGATTCTAACACCCACCCTCAAGAA : 576381 551 : sArgAlaArgAlaGlyArgGlyGluAlaThrArgGlnGluGluSerAlaGluArgSer : 569 ||||!.!|||||||||||||||||||||.!!!:!||||||||| !|||||||||||| sArgGlyArgAlaGlyArgGlyGluAlaAlaLysGlnGluGluValAlaGluArgSer 576382 : GCGGGGCCGGGCCGGCCGAGGAGAAGCAGCCAAGCAGGAGGAGGTGGCCGAGCGGTCT : 576438 570 : GluProSerGlnHisValValLeuSerLeuThrPheLysArgTyrValPheAspThrH : 589 ||||||:!!|||||||||!.!|||||||||:!!||||||||||||||||||||||||| GluProAlaGlnHisValAlaLeuSerLeuSerPheLysArgTyrValPheAspThrH 576439 : GAGCCTGCGCAGCACGTGGCCCTCAGCCTGTCTTTCAAGCGTTACGTCTTTGACACCC : 576498 590 : isLysArgMetValGlnSerPro : 596 ||||||||||||||||||||||| isLysArgMetValGlnSerPro 576499 : ACAAGCGCATGGTCCAGTCTCCC : 576521 vulgar: SPP00000144_2.0 261 596 . gi|472269049|gb|KB715608.1| 513387 576521 + 1589 M 220 660 5 0 2 I 0 16663 3 0 2 M 52 156 S 0 1 5 0 2 I 0 45458 3 0 2 S 1 2 M 62 186 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-20 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|472269049|gb|KB715608.1| exonerate:protein2genome:local gene 513388 576521 1589 + . gene_id 1 ; sequence SPP00000144_2.0 ; gene_orientation + gi|472269049|gb|KB715608.1| exonerate:protein2genome:local cds 513388 514047 . + . gi|472269049|gb|KB715608.1| exonerate:protein2genome:local exon 513388 514047 . + . insertions 0 ; deletions 0 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice5 514048 514049 . + . intron_id 1 ; splice_site "GT" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local intron 514048 530714 . + . intron_id 1 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice3 530713 530714 . + . intron_id 0 ; splice_site "AG" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local cds 530715 530871 . + . gi|472269049|gb|KB715608.1| exonerate:protein2genome:local exon 530715 530871 . + . insertions 0 ; deletions 0 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice5 530872 530873 . + . intron_id 2 ; splice_site "GT" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local intron 530872 576333 . + . intron_id 2 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice3 576332 576333 . + . intron_id 1 ; splice_site "AG" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local cds 576334 576521 . + . gi|472269049|gb|KB715608.1| exonerate:protein2genome:local exon 576334 576521 . + . insertions 0 ; deletions 0 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local similarity 513388 576521 1589 + . alignment_id 1 ; Query SPP00000144_2.0 ; Align 513388 262 660 ; Align 530715 482 156 ; Align 576336 535 186 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000144_2.0 # Protein # Eukaryotic elongation factor (eEFSec) # Human (Homo sapiens) Target: gi|472269049|gb|KB715608.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00902, whole genome shotgun sequence Model: protein2genome:local Raw score: 484 Query range: 72 -> 197 Target range: 328565 -> 587204 73 : AlaAlaProGluAlaGluProGluProGlyGluProLeuLeuGlnValThrLeuValA : 92 |||||| !!! !||||||||||||||||||||||||! !||||||||||||||||||| AlaAlaSerGlyAlaGluProGluProGlyGluProGlnLeuGlnValThrLeuValA 328566 : GCGGCGTCCGGAGCCGAGCCCGAGCCCGGCGAGCCGCAGCTTCAGGTCACGCTGGTCG : 328623 93 : spCysProGlyHisAlaSerLeuIleArgThrIleIleGly{G} >>>> Target : 106 |||||||||||||||||||||||||||||||||||||||||{|} 849 spCysProGlyHisAlaSerLeuIleArgThrIleIleGly{G}++ 328624 : ACTGCCCCGGGCACGCCTCCCTCATCCGGACCATCATTGGC{G}gt............ : 328668 107 : Intron 1 >>>> {ly}AlaGlnIleIleAspLeuMetMetLeuValIleAspVal : 119 26 bp {||}||||||||||||||||||||||||||||||||||||||| ++{ly}AlaGlnIleIleAspLeuMetMetLeuValIleAspVal 328669 : .............ag{GG}GCCCAGATCATTGATCTGATGATGCTGGTCATTGATGTG : 413630 120 : ThrLysGlyMetGlnThrGlnSerAlaGluCysLeuValIleGlyGlnIleAlaCysG : 139 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ThrLysGlyMetGlnThrGlnSerAlaGluCysLeuValIleGlyGlnIleAlaCysG 413631 : ACCAAAGGGATGCAGACCCAGTCAGCGGAGTGCCTTGTGATTGGGCAGATCGCCTGCC : 413690 140 : lnLysLeuValValValLeuAsnLysIleAspLeuLeuProGluGlyLysArgGlnAl : 158 |||||||||||||||||||||||||||||||||||||| !!||||||||||||||||| lnLysLeuValValValLeuAsnLysIleAspLeuLeuAlaGluGlyLysArgGlnAl 413691 : AGAAGCTGGTTGTGGTGCTGAACAAAATAGACCTCTTAGCTGAAGGGAAGAGACAGGC : 413747 159 : aAlaIleAspLysMetThrLysLysMetGlnLysThrLeuGluAsnThr{Ly} >>> : 175 |||||||||||||||||||||||||||||||!:!|||||||||||||||{||} aAlaIleAspLysMetThrLysLysMetGlnArgThrLeuGluAsnThr{Ly}++ 413748 : AGCGATTGATAAAATGACCAAGAAAATGCAGAGGACCCTGGAGAACACC{AA}gt... : 413802 176 : > Target Intron 2 >>>> {s}PheArgGlyAlaProIleIleProValAlaA : 186 173338 bp {|}!:!! !!.! ! ! ! ! !:!!:!!| +-{s}TyrLeuAlaTyrLeuProProLeuLeuSerA 413803 : ......................aa{A}TATCTTGCTTATTTACCTCCCTTGTTGTCAG : 587169 187 : laLysProGlyGlyProGluAlaProGluThrGlu : 197 ||...||| !||| !! !||||||||||||||| laSerProThrGlyThr***AlaProGluThrGlu 587170 : CGTCCCCCACGGGAACATGAGCTCCAGAGACAGAA : 587204 vulgar: SPP00000144_2.0 72 197 . gi|472269049|gb|KB715608.1| 328565 587204 + 484 M 33 99 S 0 1 5 0 2 I 0 84922 3 0 2 S 1 2 M 68 204 S 0 2 5 0 2 I 0 173334 3 0 2 S 1 1 M 22 66 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-20 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|472269049|gb|KB715608.1| exonerate:protein2genome:local gene 328566 587204 484 + . gene_id 2 ; sequence SPP00000144_2.0 ; gene_orientation + gi|472269049|gb|KB715608.1| exonerate:protein2genome:local cds 328566 328665 . + . gi|472269049|gb|KB715608.1| exonerate:protein2genome:local exon 328566 328665 . + . insertions 0 ; deletions 0 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice5 328666 328667 . + . intron_id 1 ; splice_site "GT" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local intron 328666 413591 . + . intron_id 1 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice3 413590 413591 . + . intron_id 0 ; splice_site "AG" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local cds 413592 413799 . + . gi|472269049|gb|KB715608.1| exonerate:protein2genome:local exon 413592 413799 . + . insertions 0 ; deletions 0 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice5 413800 413801 . + . intron_id 2 ; splice_site "GT" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local intron 413800 587137 . + . intron_id 2 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local splice3 587136 587137 . + . intron_id 1 ; splice_site "AA" gi|472269049|gb|KB715608.1| exonerate:protein2genome:local cds 587138 587204 . + . gi|472269049|gb|KB715608.1| exonerate:protein2genome:local exon 587138 587204 . + . insertions 0 ; deletions 0 gi|472269049|gb|KB715608.1| exonerate:protein2genome:local similarity 328566 587204 484 + . alignment_id 2 ; Query SPP00000144_2.0 ; Align 328566 73 99 ; Align 413594 107 204 ; Align 587139 176 66 # --- END OF GFF DUMP --- # -- completed exonerate analysis