TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000081_2.0 # Protein # Selenoprotein O (SelO) # Walrus (Odobenus rosmarus) (681 letters) Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno me.fa 16,710 sequences; 3,156,886,159 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|472269310|gb|KB715347.1| Leptonychotes weddellii isolate WS11... 206 3e-69 gi|472267791|gb|KB716866.1| Leptonychotes weddellii isolate WS11... 74 5e-11 gi|472269944|gb|KB714745.1| Leptonychotes weddellii isolate WS11... 53 6e-05 gi|472263686|gb|KB720971.1| Leptonychotes weddellii isolate WS11... 49 0.001 >gi|472269310|gb|KB715347.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00641, whole genome shotgun sequence Length = 1161464 Score = 206 bits (525), Expect(2) = 3e-69 Identities = 115/172 (66%), Positives = 116/172 (67%), Gaps = 49/172 (28%) Frame = -2 Query: 510 RQLSMMLMLAQSNPQLFALIGTRANVTKELERVEQHSRLEQLSPAELLSKNRGHWADWLQ 569 RQLSMMLMLAQSNPQLFALIGTRANVTKELERVEQHSRLEQLSPAELLSKNRGHWADWLQ Sbjct: 594721 RQLSMMLMLAQSNPQLFALIGTRANVTKELERVEQHSRLEQLSPAELLSKNRGHWADWLQ 594542 Query: 570 AY-------------------------------------------------RTRLEKDRE 580 Y R RLEKDRE Sbjct: 594541 EYG*GSRRPGADCSRHPLPGRWLQTTPPKQGTKCRRGGGVAPAGKMNLDDSRARLEKDRE 594362 Query: 581 GVSDEDAWQAERTRVMHANNPKYVLRNYIAQNAIEAAENGDFSEVRRVLKLL 632 GVSD+DAWQAERTRVMHANNPKYVLRNYIAQNAIEAAENGDFSE L LL Sbjct: 594361 GVSDKDAWQAERTRVMHANNPKYVLRNYIAQNAIEAAENGDFSEASTRLSLL 594206 Score = 189 bits (479), Expect = 7e-46 Identities = 90/101 (89%), Positives = 92/101 (91%) Frame = -2 Query: 360 PFGFLDRYDPDHVCNASDNAGRYAYSKQPEVCKWNLQKLVEALEPELPREQGEAILAEEF 419 P RYDPDHVCNASDN GRYAYSKQP+VCKWNLQKL EALEPELPRE+GE ILAEEF Sbjct: 598855 PLAPCGRYDPDHVCNASDNTGRYAYSKQPDVCKWNLQKLAEALEPELPRERGETILAEEF 598676 Query: 420 DPEFRQHYLQKMRRKLGLVHAELDEDGALVAKLLETMHLTG 460 DPEFRQHYLQKMRRKLGLV AELDEDGALVAKLLETMHLTG Sbjct: 598675 DPEFRQHYLQKMRRKLGLVRAELDEDGALVAKLLETMHLTG 598553 Score = 140 bits (353), Expect = 3e-31 Identities = 68/69 (98%), Positives = 69/69 (100%) Frame = -2 Query: 194 RQADGRKVLRSSIREFLCSEAMFHLGIPTTRAGACVTSQSTVVRDVFYDGNPKYEKCTVV 253 RQADGRKVLRSSIREFLCSEAMFHLGIPTTRAGACVTSQSTV+RDVFYDGNPKYEKCTVV Sbjct: 602020 RQADGRKVLRSSIREFLCSEAMFHLGIPTTRAGACVTSQSTVIRDVFYDGNPKYEKCTVV 601841 Query: 254 LRIASTFLR 262 LRIASTFLR Sbjct: 601840 LRIASTFLR 601814 Score = 128 bits (322), Expect = 1e-27 Identities = 58/58 (100%), Positives = 58/58 (100%) Frame = -1 Query: 137 ALLPGAEPAAHCYCGHQFGQFAGQLGDGAAMYLGEVCTAAGERWELQLKGAGPTPFSR 194 ALLPGAEPAAHCYCGHQFGQFAGQLGDGAAMYLGEVCTAAGERWELQLKGAGPTPFSR Sbjct: 606746 ALLPGAEPAAHCYCGHQFGQFAGQLGDGAAMYLGEVCTAAGERWELQLKGAGPTPFSR 606573 Score = 126 bits (316), Expect = 6e-27 Identities = 61/62 (98%), Positives = 62/62 (100%) Frame = -2 Query: 262 RFGSFEIFKSADEHTGREGPSVGRNDIRVQMLDYVISTFYPEIQAAHANDRVQRNAAFFR 321 RFGSFEIFKSADEHTGREGPSVGRNDIRVQMLDYVISTFYPEIQAAHA+DRVQRNAAFFR Sbjct: 600199 RFGSFEIFKSADEHTGREGPSVGRNDIRVQMLDYVISTFYPEIQAAHADDRVQRNAAFFR 600020 Query: 322 EV 323 EV Sbjct: 600019 EV 600014 Score = 106 bits (265), Expect = 5e-21 Identities = 52/57 (91%), Positives = 53/57 (92%) Frame = -2 Query: 454 ETMHLTGADFTNTFYLLSSFPVGPESLGLPEFLAALTAQCASLEELKLAFRPQMDPR 510 E + GADFTNTFYLLSSFPVGPESLGLPEFLAALTAQCASLEELKLAFRPQMDPR Sbjct: 595696 EQRRVPGADFTNTFYLLSSFPVGPESLGLPEFLAALTAQCASLEELKLAFRPQMDPR 595526 Score = 98.2 bits (243), Expect = 2e-18 Identities = 44/45 (97%), Positives = 45/45 (100%) Frame = -3 Query: 322 EVTRRTARMVAEWQCVGFCHGVLNTDNMSIVGLTIDYGPFGFLDR 366 +VTRRTARMVAEWQCVGFCHGVLNTDNMSIVGLTIDYGPFGFLDR Sbjct: 599391 QVTRRTARMVAEWQCVGFCHGVLNTDNMSIVGLTIDYGPFGFLDR 599257 Score = 82.0 bits (201), Expect(2) = 3e-69 Identities = 41/58 (70%), Positives = 42/58 (72%) Frame = -3 Query: 624 EVRRVLKLLETPYHRDXXXXXXXXXXXXXXADGADSGGHSYSSKPPLWAAELCVTXSS 681 +VRRVLKLLETPYHRD A GADSGGHSYSSKPPLWAAELCVT SS Sbjct: 594129 QVRRVLKLLETPYHRDGEATEDLEATEPEGAGGADSGGHSYSSKPPLWAAELCVT*SS 593956 >gi|472267791|gb|KB716866.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold02160, whole genome shotgun sequence Length = 521749 Score = 73.6 bits (179), Expect = 5e-11 Identities = 51/131 (38%), Positives = 58/131 (44%), Gaps = 59/131 (45%) Frame = +1 Query: 154 FGQFAGQLGDGAAMYLGEV-----------------------------CTAAGERWELQL 184 FG +AGQLGDG A LG C GE+WELQL Sbjct: 386635 FGIWAGQLGDGRAHLLGIYMNRYHMFSGIIAFLLNINTDLLILRFLCCC*RQGEKWELQL 386814 Query: 185 KGAGPTPFSRQA------------------------------DGRKVLRSSIREFLCSEA 214 KG+G TP+SR + DGR VLRSS+REFLCSEA Sbjct: 386815 KGSGKTPYSRYSLIFFWSSK*KLILSSLQLKIFLLLFCPRNGDGRAVLRSSVREFLCSEA 386994 Query: 215 MFHLGIPTTRA 225 M LGIPT+RA Sbjct: 386995 MHSLGIPTSRA 387027 Score = 41.2 bits (95), Expect = 0.25 Identities = 20/35 (57%), Positives = 28/35 (80%) Frame = +3 Query: 598 ANNPKYVLRNYIAQNAIEAAENGDFSEVRRVLKLL 632 A NP+YVL+N++A++A+ AE DFSEV R L+LL Sbjct: 391584 AVNPRYVLKNWMAESAVRKAERNDFSEV-RCLRLL 391685 >gi|472269944|gb|KB714745.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00039, whole genome shotgun sequence Length = 4111496 Score = 53.1 bits (126), Expect = 6e-05 Identities = 29/50 (58%), Positives = 33/50 (66%) Frame = +1 Query: 460 GADFTNTFYLLSSFPVGPESLGLPEFLAALTAQCASLEELKLAFRPQMDP 509 GAD TNTFYLL S PESLGL + Q ASLEEL+L F+ Q+DP Sbjct: 184771 GADITNTFYLLISLSAVPESLGLTLSSQRI*LQGASLEELRLPFQSQVDP 184920 >gi|472263686|gb|KB720971.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold06266, whole genome shotgun sequence Length = 51140 Score = 48.9 bits (115), Expect = 0.001 Identities = 19/36 (52%), Positives = 25/36 (69%) Frame = -1 Query: 334 WQCVGFCHGVLNTDNMSIVGLTIDYGPFGFLDRYDP 369 W GV NTDN S++ +TIDYGPFGF++ Y+P Sbjct: 17510 WNLFVVVSGVCNTDNFSLLSITIDYGPFGFMEAYNP 17403 Score = 41.2 bits (95), Expect = 0.25 Identities = 20/35 (57%), Positives = 28/35 (80%) Frame = -2 Query: 598 ANNPKYVLRNYIAQNAIEAAENGDFSEVRRVLKLL 632 A NP+YVL+N++A++A+ AE DFSEV R L+LL Sbjct: 2782 AVNPRYVLKNWMAESAVRKAERNDFSEV-RCLRLL 2681 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 3,087,506,849 Number of sequences in database: 5788 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa.01 Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 69,379,310 Number of sequences in database: 10,922 Lambda K H 0.321 0.135 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 2,223,263,993 Number of Sequences: 16710 Number of extensions: 30876615 Number of successful extensions: 125862 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 361 Number of HSP's successfully gapped in prelim test: 329 Number of HSP's that attempted gapping in prelim test: 120776 Number of HSP's gapped (non-prelim): 16980 length of query: 681 length of database: 1,052,295,386 effective HSP length: 139 effective length of query: 542 effective length of database: 1,049,972,696 effective search space: 569085201232 effective search space used: 569085201232 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 81 (35.8 bits)